Explanipedia

Lipidomic Profiling of Arabidopsis Chloroplast Protein Phosphatase Slp1 Mutants Reveals Altered Diurnal Lipid Remodeling Open

Greg B. G. Moorhead, Chris White-Gloria, Adriana Zardini Buzatto · 2025

Plant acetyl-CoA carboxylase: The homomeric form and the heteromeric form Open

Dilawar Niazi, Greg B. G. Moorhead · 2025

Phospho-proteomics identifies D-group MAP kinases as substrates of the Arabidopsis tyrosine phosphatase RLPH2 Open

Anne‐Marie Labandera, Ryan Toth, Sierra Mitchell, Jayde J. Johnson, Juliette Puyaubert , et al. · 2024

Despite being one of the few bona fide plant tyrosine phosphatases, RLPH2 has no known substrates. Utilizing phospho-proteomics, we identified the activation loop phospho-tyrosine of several D-group mitogen activated protein kinases (MPKs)…

Abstract 1639 Chloroplast protein phosphatase SLP1 counters protein kinase, casein kinase 2 Open

Greg B. G. Moorhead · 2024

The serine/threonine protein phosphatase SLP1 is targeted to the higher plant chloroplast and has no known substrates. We have performed quantitative phosphoproteomics on wildtype, SLP1 knockout and over-expressor lines to uncover substrat…

Abstract 2268 Protein phosphatase SLP1 is a novel regulator of chloroplastic metabolism Open

Chris White-Gloria, Greg B. G. Moorhead · 2024

Photosynthesis is one of the most important biological phenomena on the planet. One regulatory mechanism for this process is the post-translational modification of proteins involved in catalyzing the light (in)dependent reactions occurring…

Eukaryotic-like phosphoprotein phosphatase (PPP) enzyme evolution: interactions with environmental toxins and regulatory proteins Open

David Kerk, Chris White-Gloria, Jayde J. Johnson, Greg B. G. Moorhead · 2023

Phosphoprotein phosphatases (PPPs) are a ubiquitous class of enzymes which dephosphorylate serine and threonine residues on substrate proteins involved in a wide variety of cellular processes. The active site of PPP enzymes are highly cons…

Subcellular distribution of PP1 isoforms in holoenzyme complexes Open

Virja Mehta, Delphine Chamousset, Jennifer Law, Sarah Ooi, Denise Campuzano , et al. · 2022

Unlike its counterpart Ser/Thr kinases, the predominant Ser/Thr protein phosphatase 1 (PP1) is a promiscuous enzyme that gains its subcellular localization and substrate specificity from a large panel of regulatory proteins with which it a…

Identification of Arabidopsis Protein Kinases That Harbor Functional Type 1 Peroxisomal Targeting Signals Open

Amr R. A. Kataya, Nitija Gautam, Muhammad Jamshed, Douglas G. Muench, Marcus A. Samuel , et al. · 2022

Peroxisomes are eukaryotic specific organelles that perform diverse metabolic functions including fatty acid β-oxidation, reactive species metabolism, photorespiration, and responses to stress. However, the potential regulation of these fu…

Affinity-based profiling of endogenous phosphoprotein phosphatases by mass spectrometry Open

Brooke L. Brauer, Kwame Wiredu, Sierra Mitchell, Greg B. G. Moorhead, Scott A. Gerber , et al. · 2021

The origin and radiation of the phosphoprotein phosphatase (PPP) enzymes of Eukaryotes Open

David Kerk, Jordan F. Mattice, Mario E. Valdés‐Tresanco, Sergei Y. Noskov, Kenneth Ng , et al. · 2021

Origin of the Phosphoprotein Phosphatase (PPP) sequence family in Bacteria: Critical ancestral sequence changes, radiation patterns and substrate binding features Open

David Kerk, Mario E. Valdés‐Tresanco, Ryan Toth, Sergei Y. Noskov, Kenneth Ng , et al. · 2021

LIKE SEX4 1 Acts as a β-Amylase-Binding Scaffold on Starch Granules during Starch Degradation Open

Tina B. Schreier, Martin Umhang, Sang‐Kyu Lee, Wei‐Ling Lue, Zhouxin Shen , et al. · 2019

In Arabidopsis (Arabidopsis thaliana) leaves, starch is synthesized during the day and degraded at night to fuel growth and metabolism. Starch is degraded primarily by β-amylases, liberating maltose, but this activity is preceded by…

A Quantitative Chemical Proteomic Strategy for Profiling Phosphoprotein Phosphatases from Yeast to Humans Open

Scott P. Lyons, Nicole P. Jenkins, Isha Nasa, M.S. Choy, Mark E. Adamo , et al. · 2018

A "tug-of-war" between kinases and phosphatases establishes the phosphorylation states of proteins. While serine and threonine phosphorylation can be catalyzed by more than 400 protein kinases, the majority of serine and threonine dephosph…

Crystal structures of the RLPH2 protein phosphatase from <i>Arabidopsis thaliana</i> reveal a novel mechanism for recognizing dually phosphorylated substrates Open

Anne‐Marie Labandera, R. Glen Uhrig, Keaton Colville, Greg B. G. Moorhead, Kenneth Ng · 2018

Despite belonging to the phosphoserine-and phosphothreonine-specific phosphoprotein phosphatase (PPP) family, Arabidopsis thaliana Rhizobiales-like phosphatase 2 (RLPH2) strongly prefers substrates bearing phosphorylated tyrosine residues.…

Protein Kinases and Phosphatases of the Plastid and Their Potential Role in Starch Metabolism Open

Chris White-Gloria, Jayde J. Johnson, Kayla Marritt, Amr R. A. Kataya, Ahmad Reza Vahab , et al. · 2018

Phospho-proteomic studies have confirmed that phosphorylation is a common mechanism to regulate protein function in the chloroplast, including the enzymes of starch metabolism. In addition to the photosynthetic machinery protein kinases (S…

Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins Open

Isha Nasa, Scott Rusin, Arminja N. Kettenbach, Greg B. G. Moorhead · 2018

Phosphorylation within conserved motifs in regulatory subunits controls protein phosphatase 1 (PP1) holoenzyme assembly during mitosis.

Lysine acetylome profiling uncovers novel histone deacetylase substrate proteins in Arabidopsis Open

Markus Hartl, Magdalena Füßl, Paul J. Boersema, Jan Oliver Jost, Katharina Kramer , et al. · 2017

Histone deacetylases have central functions in regulating stress defenses and development in plants. However, the knowledge about the deacetylase functions is largely limited to histones, although these enzymes were found in diverse subcel…

Lysine acetylome profiling uncovers novel histone deacetylase substrate proteins in <i>Arabidopsis</i> Open

Markus Hartl, Magdalena Füßl, Paul J. Boersema, Jan Oliver Jost, Katharina Kramer , et al. · 2017

AtSLP2 is an intronless protein phosphatase that co-expresses with intronless mitochondrial pentatricopeptide repeat (PPR) and tetratricopeptide (TPR) protein encoding genes Open

R. Glen Uhrig, Greg B. G. Moorhead · 2017

Shewanella-like PPP family phosphatases (SLPs) are a unique lineage of eukaryote PPP-family phosphatases of bacterial origin which are not found in metazoans.^1,2 Their absence in metazoans is marked by their ancient bacterial or…

Recruitment of PP1 to the centrosomal scaffold protein CEP192 Open

Isha Nasa, Laura Trinkle‐Mulcahy, Pauline Douglas, Sibapriya Chaudhuri, Susan P. Lees‐Miller , et al. · 2017

AtSLP2 is an intronless protein phosphatase that co-expresses with intronless mitochondrial pentatricopeptide repeat (PPR) and tetratricopeptide (TPR) protein encoding genes Open

R. Glen Uhrig, Greg B. G. Moorhead · 2017

Shewanella-like PPP family phosphatases (SLPs) are a unique lineage of eukaryote PPP-family phosphatases of bacterial origin which are not found in metazoans.^1,2 Their absence in metazoans is marked by their ancient bacterial or…

Activation of Mitochondrial Protein Phosphatase SLP2 by MIA40 Regulates Seed Germination Open

R. Glen Uhrig, Anne‐Marie Labandera, Lay-Yin Tang, Nicolas Sieben, Marilyn Goudreault , et al. · 2016

Reversible protein phosphorylation catalyzed by protein kinases and phosphatases represents the most prolific and well-characterized posttranslational modification known. Here, we demonstrate that Arabidopsis (Arabidopsis thaliana) Shewane…

Rhizobiales-like Phosphatase 2 from Arabidopsis thaliana Is a Novel Phospho-tyrosine-specific Phospho-protein Phosphatase (PPP) Family Protein Phosphatase Open

R. Glen Uhrig, Anne‐Marie Labandera, Muhammad Jamshed, Marcus A. Samuel, Greg B. G. Moorhead · 2016

"PP2C7s", Genes Most Highly Elaborated in Photosynthetic Organisms, Reveal the Bacterial Origin and Stepwise Evolution of PPM/PP2C Protein Phosphatases Open

David Kerk, Dylan M. Silver, R. Glen Uhrig, Greg B. G. Moorhead · 2015

Mg+2/Mn+2-dependent type 2C protein phosphatases (PP2Cs) are ubiquitous in eukaryotes, mediating diverse cellular signaling processes through metal ion catalyzed dephosphorylation of target proteins. We have identified a distinct PP2C sequ…

Actin dynamics tune the integrated stress response by regulating eukaryotic initiation factor 2α dephosphorylation Open

Joseph E. Chambers, Lucy E. Dalton, Hanna J. Clarke, Elke Malzer, Caia Dominicus , et al. · 2015

Four stress-sensing kinases phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 (eIF2α) to activate the integrated stress response (ISR). In animals, the ISR is antagonised by selective eIF2α phosphatases comprisi…

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