B. Sekula
YOU?
Author Swipe
View article: Molecular basis of antibiotic sensing by the <scp>TetR</scp> family regulator <scp>CecR</scp> – a structural perspective
Molecular basis of antibiotic sensing by the <span>TetR</span> family regulator <span>CecR</span> – a structural perspective Open
Escherichia coli HTH‐type transcriptional dual regulator CecR belongs to TetR family regulators (TFRs), which regulate the expression of genes enabling bacteria to survive under stress conditions. Previous studies (Yamanaka et al. , Microb…
View article: Arabidopsis thaliana argininosuccinate lyase structure uncovers the role of serine as the catalytic base
Arabidopsis thaliana argininosuccinate lyase structure uncovers the role of serine as the catalytic base Open
Arginine is an important amino acid in plants, as it not only plays a structural role and serves as nitrogen storage but is also a precursor for various molecules, including polyamines and proline. Arginine is produced by argininosuccinate…
View article: Comprehensive structural overview of the C-terminal ligand-binding domains of the TetR family regulators
Comprehensive structural overview of the C-terminal ligand-binding domains of the TetR family regulators Open
TetR family regulators (TFRs) represent a large group of one-component bacterial signal transduction systems which recognize environmental signals, like the presence of antibiotics or other bactericidal compounds, and trigger the cell resp…
View article: Structural analysis and molecular substrate recognition properties of Arabidopsis thaliana ornithine transcarbamylase, the molecular target of phaseolotoxin produced by Pseudomonas syringae
Structural analysis and molecular substrate recognition properties of Arabidopsis thaliana ornithine transcarbamylase, the molecular target of phaseolotoxin produced by Pseudomonas syringae Open
Halo blight is a plant disease that leads to a significant decrease in the yield of common bean crops and kiwi fruits. The infection is caused by Pseudomonas syringae pathovars that produce phaseolotoxin, an antimetabolite which targets ar…
View article: Structural and functional studies of <i>Bacillus subtilis</i> LonBA, member of a newly identified subfamily of Lon proteases
Structural and functional studies of <i>Bacillus subtilis</i> LonBA, member of a newly identified subfamily of Lon proteases Open
ATP-dependent Lon proteases are bifunctional, multidomain, homooligomeric enzymes that play an important role in cellular protein quality control, by being responsible for degradation of abnormal, misfolded, and some regulatory proteins.Th…
View article: Unique Structural Fold of LonBA Protease from Bacillus subtilis, a Member of a Newly Identified Subfamily of Lon Proteases
Unique Structural Fold of LonBA Protease from Bacillus subtilis, a Member of a Newly Identified Subfamily of Lon Proteases Open
ATP-dependent Lon proteases are key participants in the quality control system that supports the homeostasis of the cellular proteome. Based on their unique structural and biochemical properties, Lon proteases have been assigned in the MER…
View article: Arabidopsis thaliana serine hydroxymethyltransferases: functions, structures, and perspectives
Arabidopsis thaliana serine hydroxymethyltransferases: functions, structures, and perspectives Open
Serine hydroxymethyltransferase (SHM) is one of the hallmarks of one-carbon metabolism. In plants, isoforms of SHM participate in photorespiration and/or transfer the one-carbon unit from L-serine to tetrahydrofolate (THF), hence producing…
View article: Serendipitous crystallization of E. coli HPII catalase, a sequel to “the tale usually not told”
Serendipitous crystallization of E. coli HPII catalase, a sequel to “the tale usually not told” Open
Protein crystallographers are well aware of the trap of crystallizing E. coli proteins instead of the macromolecule of interest if heterologous recombinant protein expression in E. coli was part of the experimental pipeline. Among the well…
View article: Base pairing, structural and functional insights into N4-methylcytidine (m4C) and N4,N4-dimethylcytidine (m42C) modified RNA
Base pairing, structural and functional insights into N4-methylcytidine (m4C) and N4,N4-dimethylcytidine (m42C) modified RNA Open
The N4-methylation of cytidine (m4C and m42C) in RNA plays important roles in both bacterial and eukaryotic cells. In this work, we synthesized a series of m4C and m42C modified RNA oligonucleotides, conducted their base pairing and bioact…
View article: The Neighboring Subunit Is Engaged to Stabilize the Substrate in the Active Site of Plant Arginases
The Neighboring Subunit Is Engaged to Stabilize the Substrate in the Active Site of Plant Arginases Open
Arginine acts as a precursor of polyamines in plants in two known pathways, agmatine and ornithine routes. It is decarboxylated to agmatine by arginine decarboxylase, and then transformed to putrescine by the consecutive action of agmatine…
View article: Spermidine Synthase (SPDS) Undergoes Concerted Structural Rearrangements Upon Ligand Binding – A Case Study of the Two SPDS Isoforms From Arabidopsis thaliana
Spermidine Synthase (SPDS) Undergoes Concerted Structural Rearrangements Upon Ligand Binding – A Case Study of the Two SPDS Isoforms From Arabidopsis thaliana Open
Spermidine synthases (SPDSs) catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopr…
View article: Structural Study of Agmatine Iminohydrolase From Medicago truncatula, the Second Enzyme of the Agmatine Route of Putrescine Biosynthesis in Plants
Structural Study of Agmatine Iminohydrolase From Medicago truncatula, the Second Enzyme of the Agmatine Route of Putrescine Biosynthesis in Plants Open
Plants are unique eukaryotes that can produce putrescine (PUT), a basic diamine, from arginine via a three-step pathway. This process starts with arginine decarboxylase that converts arginine to agmatine. Then, the consecutive action of tw…
View article: Structural Analysis of Phosphoserine Aminotransferase (Isoform 1) From Arabidopsis thaliana– the Enzyme Involved in the Phosphorylated Pathway of Serine Biosynthesis
Structural Analysis of Phosphoserine Aminotransferase (Isoform 1) From Arabidopsis thaliana– the Enzyme Involved in the Phosphorylated Pathway of Serine Biosynthesis Open
Phosphoserine aminotransferase (PSAT) is a pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes the conversion of 3-phosphohydroxypyruvate (3-PHP) to 3-phosphoserine (PSer) in an L-glutamate (Glu)-linked reversible transamination r…
View article: Chloroplastic Serine Hydroxymethyltransferase From Medicago truncatula: A Structural Characterization
Chloroplastic Serine Hydroxymethyltransferase From Medicago truncatula: A Structural Characterization Open
Serine hydroxymethyltransferase (SHMT, EC 2.1.2.1) is a pyridoxal 5'-phosphate (PLP)-dependent enzyme which catalyzes the reversible serine-to-glycine conversion in either a tetrahydrofolate-dependent or -independent manner. The enzyme is …
View article: Crystal structure of thermospermine synthase from <i>Medicago truncatula</i> and substrate discriminatory features of plant aminopropyltransferases
Crystal structure of thermospermine synthase from <i>Medicago truncatula</i> and substrate discriminatory features of plant aminopropyltransferases Open
Polyamines are linear polycationic compounds that play a crucial role in the growth and development of higher plants. One triamine (spermidine, SPD) and two tetraamine isomers (spermine, SPM, and thermospermine, TSPM) are obtained by the t…
View article: Structural evidence of the species-dependent albumin binding of the modified cyclic phosphatidic acid with cytotoxic properties
Structural evidence of the species-dependent albumin binding of the modified cyclic phosphatidic acid with cytotoxic properties Open
Cyclic phosphatidic acids (cPAs) are naturally occurring, very active signalling molecules, which are involved in several pathological states, such as cancer, diabetes or obesity. As molecules of highly lipidic character found in the circu…
View article: Structural Investigations of N-carbamoylputrescine Amidohydrolase from Medicago truncatula: Insights into the Ultimate Step of Putrescine Biosynthesis in Plants
Structural Investigations of N-carbamoylputrescine Amidohydrolase from Medicago truncatula: Insights into the Ultimate Step of Putrescine Biosynthesis in Plants Open
Putrescine, 1,4-diaminobutane, is an intermediate in the biosynthesis of more complexed polyamines, spermidine and spermine. Unlike other eukaryotes, plants have evolved a multistep pathway for putrescine biosynthesis that utilizes arginin…