Barbara Ciani
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View article: Mapping EGFR1 sorting domains in endosomes with a calibrated 3D expansion microscopy toolkit
Mapping EGFR1 sorting domains in endosomes with a calibrated 3D expansion microscopy toolkit Open
Endosomes are nanoscale intracellular compartments that sort and recycle cell surface receptors such as epidermal growth factor receptor 1 (EGFR1). Nanometre-scale interactions and co-clustering of signalling proteins, cargo, and the membr…
View article: CD9 co-operation with syndecan-1 is required for a major staphylococcal adhesion pathway
CD9 co-operation with syndecan-1 is required for a major staphylococcal adhesion pathway Open
Epithelial colonization is a critical first step in bacterial pathogenesis. Staphylococcus aureus can utilize several host factors to associate with cells, including α5β1 integrin and heparan sulfate proteoglycans, such as the syndecans. H…
View article: CD9 co-operation with syndecan-1 is required for a major staphylococcal adhesion pathway
CD9 co-operation with syndecan-1 is required for a major staphylococcal adhesion pathway Open
Objectives Epithelial colonisation is a critical first step in bacterial pathogenesis. Staphylococcus aureus can utilise several host factors to associate with cells, including α5βl integrin and heparan sulphate proteoglycans, such as the …
View article: Progesterone receptor expression contributes to gemcitabine resistance at higher ECM stiffness in breast cancer cell lines
Progesterone receptor expression contributes to gemcitabine resistance at higher ECM stiffness in breast cancer cell lines Open
Chemoresistance poses a great barrier to breast cancer treatment and is thought to correlate with increased matrix stiffness. We developed two-dimensional (2D) polyacrylamide (PAA) and three-dimensional (3D) alginate in vitro models of tis…
View article: Direct binding of ESCRT protein Chm7 to phosphatidic acid–rich membranes at nuclear envelope herniations
Direct binding of ESCRT protein Chm7 to phosphatidic acid–rich membranes at nuclear envelope herniations Open
Mechanisms that control nuclear membrane remodeling are essential to maintain the integrity of the nucleus but remain to be fully defined. Here, we identify a phosphatidic acid (PA)–binding capacity in the nuclear envelope (NE)–specific ES…
View article: The influence of phosphatidylserine localisation and lipid phase on membrane remodelling by the ESCRT-II/ESCRT-III complex
The influence of phosphatidylserine localisation and lipid phase on membrane remodelling by the ESCRT-II/ESCRT-III complex Open
ESCRT-II/III machinery generates intraluminal vesicles with liquid ordered membranes but requires liquid disordered membrane in the bud neck for efficiency.
View article: Direct PA-binding by Chm7 is required for nuclear envelope surveillance at herniations
Direct PA-binding by Chm7 is required for nuclear envelope surveillance at herniations Open
Mechanisms that control nuclear membrane remodeling are essential to maintain the integrity of the nucleus but remain to be fully defined. Here, we identify a phosphatidic acid (PA)-binding activity in the nuclear envelope-specific ESCRT, …
View article: The influence of phosphatidylserine localisation and lipid phase on membrane remodelling by the ESCRT-II/ESCRT-III complex
The influence of phosphatidylserine localisation and lipid phase on membrane remodelling by the ESCRT-II/ESCRT-III complex Open
The endosomal sorting complex required for transport (ESCRT) organises in supramolecular structures on the surface of lipid bilayers to drive membrane invagination and scission of intraluminal vesicles (ILVs), a process also controlled by …
View article: Dataset associated with 'In vitro membrane remodelling by ESCRT-II/ESCRT-III is regulated by negative feedback from membrane tension'
Dataset associated with 'In vitro membrane remodelling by ESCRT-II/ESCRT-III is regulated by negative feedback from membrane tension' Open
Data associated with publication of this title in the iScience journal, CellPress, 2019
View article: ESCRT-III accumulates in micronuclei with ruptured nuclear envelopes
ESCRT-III accumulates in micronuclei with ruptured nuclear envelopes Open
1. Abstract Micronuclei represent the cellular attempt to compartmentalize DNA to maintain genomic integrity threatened by mitotic errors and genotoxic events. Micronuclei show aberrant nuclear envelopes that collapse, generating damaged D…
View article: <i>In vitro</i> membrane remodelling by ESCRT-II/ESCRT-III is regulated by negative feedback from membrane tension
<i>In vitro</i> membrane remodelling by ESCRT-II/ESCRT-III is regulated by negative feedback from membrane tension Open
Artificial cells can shed new light on the molecular basis for life and hold potential for new chemical technologies. Inspired by how nature dynamically regulates its membrane compartments, we aim to repurpose the endosomal sorting complex…
View article: Membrane remodelling by a lipidated endosomal sorting complex required for transport-III chimera, <i>in vitro</i>
Membrane remodelling by a lipidated endosomal sorting complex required for transport-III chimera, <i>in vitro</i> Open
The complexity of eukaryotic cells is underscored by the compartmentalization of chemical signals by phospholipid membranes. A grand challenge of synthetic biology is building life from the ‘bottom-up’, for the purpose of generating system…
View article: The artificial cell: biology-inspired compartmentalization of chemical function
The artificial cell: biology-inspired compartmentalization of chemical function Open
The concept of compartmentalization in artificial cell research was the focus of a Royal Society Theo Murphy meeting at the Kavli Royal Society Centre, Chichelely Hall in Buckinghamshire, UK, 26–27 February 2018.
View article: A conserved loop–wedge motif moderates reaction site search and recognition by FEN1
A conserved loop–wedge motif moderates reaction site search and recognition by FEN1 Open
DNA replication and repair frequently involve intermediate two-way junction structures with overhangs, or flaps, that must be promptly removed; a task performed by the essential enzyme flap endonuclease 1 (FEN1). We demonstrate a functiona…
View article: pH dependent binding in <i>de novo</i> hetero bimetallic coiled coils
pH dependent binding in <i>de novo</i> hetero bimetallic coiled coils Open
pH dependent metal binding to a designed hetero bimetallic coiled coil featuring a lanthanide binding site.
View article: Supplementary material from "Membrane remodelling by a lipidated endosomal sorting complex required for transport-III chimera, <i>in vitro</i>"
Supplementary material from "Membrane remodelling by a lipidated endosomal sorting complex required for transport-III chimera, <i>in vitro</i>" Open
The complexity of eukaryotic cells is underscored by the compartmentalization of chemical signals by phospholipid membranes. A grand challenge of synthetic biology is building life from the ‘bottom-up’, for the purpose of generating system…
View article: C5orf30 is a negative regulator of tissue damage in rheumatoid arthritis
C5orf30 is a negative regulator of tissue damage in rheumatoid arthritis Open
Significance Recent studies have reported genetic association of chromosome 5 open reading frame 30 ( C5orf30 ) with both rheumatoid arthritis (RA) susceptibility and the severity of radiological damage to joints. The gene is expressed in …
View article: Nature's lessons in design: nanomachines to scaffold, remodel and shape membrane compartments
Nature's lessons in design: nanomachines to scaffold, remodel and shape membrane compartments Open
Our understanding of the membrane sculpting capabilities of proteins from experimental model systems could be used to construct functional compartmentalised architectures for the engineering of synthetic cells.