Barend Christiaan Vorster
YOU?
Author Swipe
View article: Biallelic variants in RYR1 and STAC3 are predominant causes of King-Denborough Syndrome in an African cohort
Biallelic variants in RYR1 and STAC3 are predominant causes of King-Denborough Syndrome in an African cohort Open
King-Denborough Syndrome (KDS) is a congenital myopathy (CM) characterised by myopathy, dysmorphic features and susceptibility to malignant hyperthermia. The objective of this study was to investigate the genotype-phenotype correlation in …
View article: Biosensor based on cobalt tungstate zinc phthalocyanine-glucose oxidase composite
Biosensor based on cobalt tungstate zinc phthalocyanine-glucose oxidase composite Open
View article: Newborn screening in South Africa: the past, present, and plans for the future
Newborn screening in South Africa: the past, present, and plans for the future Open
Worldwide, comprehensive newborn screening (NBS) now includes a clinical examination at birth, hearing screening, pulse oximetry measurement for congenital heart defects, and biochemical screening to identify congenital disorders early in …
View article: Advancing diagnosis and research for rare genetic diseases in Indigenous peoples
Advancing diagnosis and research for rare genetic diseases in Indigenous peoples Open
View article: Clinical, biochemical, and genetic spectrum of MADD in a South African cohort: an ICGNMD study
Clinical, biochemical, and genetic spectrum of MADD in a South African cohort: an ICGNMD study Open
View article: The advantages of rare disease biobanking: A localised source of genetic knowledge to benefit the South African rare disease community and related stakeholders worldwide
The advantages of rare disease biobanking: A localised source of genetic knowledge to benefit the South African rare disease community and related stakeholders worldwide Open
-
View article: Quantitative Galactose Colorimetric Competitive Assay Based on Galactose Dehydrogenase and Plasmonic Gold Nanostars
Quantitative Galactose Colorimetric Competitive Assay Based on Galactose Dehydrogenase and Plasmonic Gold Nanostars Open
We describe a competitive colorimetric assay that enables rapid and sensitive detection of galactose and reduced nicotinamide adenine dinucleotide (NADH) via colorimetric readouts and demonstrate its usefulness for monitoring NAD+-driven e…
View article: Quantitative Galactose Colorimetric Competitive Assay Based on Galactose Dehydrogenase and Plasmonic Gold Nanostars
Quantitative Galactose Colorimetric Competitive Assay Based on Galactose Dehydrogenase and Plasmonic Gold Nanostars Open
We describe a competitive colorimetric assay that enables rapid and sensitive detection of galactose and reduced nicotinamide adenine dinucleotide (NADH) via colorimetric readouts and demon-strates its usefulness for monitoring NAD+-driven…
View article: Feasibility of NAD(P)/NAD(P)H as redox agents in enzymatic plasmonic gold nanostar assays for galactose quantification
Feasibility of NAD(P)/NAD(P)H as redox agents in enzymatic plasmonic gold nanostar assays for galactose quantification Open
Plasmonic colorimetric sensors have emerged as powerful analytical tools in biochemistry due to their localized surface plasmon resonance extinction in the visible range. Here, we describe the feasibility of NAD(P)/NAD(P)H as redox agents …
View article: Non-Enzymatic Formation of N-acetylated Amino Acid Conjugates in Urine
Non-Enzymatic Formation of N-acetylated Amino Acid Conjugates in Urine Open
Unknown N-acylated amino acid (N-AAA) conjugates have been detected in maple syrup urine disease (MSUD) and other inborn errors of metabolism (IEMs). This study aimed to elucidate the mechanism behind the formation of urinary N-AAA conjuga…
View article: Clinical, biochemical, and genetic spectrum of MADD in a South African cohort: an ICGNMD study
Clinical, biochemical, and genetic spectrum of MADD in a South African cohort: an ICGNMD study Open
Background Multiple acyl-CoA dehydrogenase deficiency (MADD) is an autosomal recessive disorder resulting from pathogenic variants in three distinct genes, with most of the variants occurring in ETFDH . Recent evidence of potential founder…
View article: Miniaturization and Automation Protocol of a Urinary Organic Acid Liquid-Liquid Extraction Method on GC-MS
Miniaturization and Automation Protocol of a Urinary Organic Acid Liquid-Liquid Extraction Method on GC-MS Open
The aim of this study was to improve the extraction method for urinary organic acids by miniaturizing and automating the process. Currently, manual extraction methods are commonly used, which can be time-consuming and lead to variations in…
View article: The Effect of Capping Agents on Gold Nanostar Stability, Functionalization, and Colorimetric Biosensing Capability
The Effect of Capping Agents on Gold Nanostar Stability, Functionalization, and Colorimetric Biosensing Capability Open
Capping agents (organic ligands, polymers, and surfactants) are pivotal for stabilizing nanoparticles; however, they may influence the surface chemistry, as well as the physico-chemical and biological characteristics, of gold nanostar (AuN…
View article: Serum Metabolomic and Lipoprotein Profiling of Pancreatic Ductal Adenocarcinoma Patients of African Ancestry
Serum Metabolomic and Lipoprotein Profiling of Pancreatic Ductal Adenocarcinoma Patients of African Ancestry Open
Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer with a characteristic dysregulated metabolism. Abnormal clinicopathological features linked to defective metabolic and inflammatory response pathways can induce PDAC development an…
View article: Metabolic and Lipoprotein Profiling of Pancreatic Ductal Adenocarcinoma Patients of African Ancestry
Metabolic and Lipoprotein Profiling of Pancreatic Ductal Adenocarcinoma Patients of African Ancestry Open
Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer with a characteristic dysregulated metabolism. Abnormal clinicopathological features linked to defective metabolic and inflammatory response pathways can induce PDAC development an…
View article: Randomized control trial of oral arginine therapy for children with sickle cell anemia hospitalized for pain in <scp>Nigeria</scp>
Randomized control trial of oral arginine therapy for children with sickle cell anemia hospitalized for pain in <span>Nigeria</span> Open
Low arginine bioavailability is associated with vaso‐occlusive painful crisis (VOC) severity in sickle cell anemia (SCA) and predicts need for pediatric hospitalization. Intravenous arginine therapy has opioid‐sparing effects and was found…
View article: A call for global action for rare diseases in Africa
A call for global action for rare diseases in Africa Open
View article: Gold Nanostar Colorimetric Detection of Fructosyl Valine as a Potential Future Point of Care Biosensor Candidate for Glycated Haemoglobin Detection
Gold Nanostar Colorimetric Detection of Fructosyl Valine as a Potential Future Point of Care Biosensor Candidate for Glycated Haemoglobin Detection Open
Diabetes Mellitus is a growing global concern. The current methods used to detect glycated haemoglobin are precise, however, utilise expensive equipment, reagents and consumables. These are luxuries which rural communities cannot access. T…
View article: Tailor-made gold nanostar colorimetric detection determined by morphology change and used as an indirect approach by using hydrogen peroxide to determine glucose concentration
Tailor-made gold nanostar colorimetric detection determined by morphology change and used as an indirect approach by using hydrogen peroxide to determine glucose concentration Open
Gold nanostars are being utilized more regularly in the field of nanodiagnostics. The modified seedless synthetic
\nmethod comprised of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) synthesized with the addition
\nof silver ni…
View article: Plasmonic Detection of Glucose in Serum Based on Biocatalytic Shape-Altering of Gold Nanostars
Plasmonic Detection of Glucose in Serum Based on Biocatalytic Shape-Altering of Gold Nanostars Open
Nanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-effective with easy readout formats for clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-a…
View article: Modified HEPES one-pot synthetic strategy for gold nanostars
Modified HEPES one-pot synthetic strategy for gold nanostars Open
Gold nanostars are being used more regularly in the biosensing field. Despite their useful attributes, there is still a need to optimize aspects of the synthesis and stability. The seedless, synthetic method comprising 4-(2-hydroxyethyl)-1…
View article: Facile immobilization of glucose oxidase onto gold nanostars with enhanced binding affinity and optimal function
Facile immobilization of glucose oxidase onto gold nanostars with enhanced binding affinity and optimal function Open
Gold nanoparticles provide a user-friendly and efficient surface for immobilization of enzymes and proteins. In this paper, we present a novel approach for enzyme bioconjugation to gold nanostars (AuNSs). AuNSs were modified with l -cystei…
View article: Seedless gold nanostars with seed-like advantages for biosensing applications
Seedless gold nanostars with seed-like advantages for biosensing applications Open
Gold nanostars (AuNSs) are seen as promising building blocks for biosensors with potential for easy readouts based on naked-eye and ultraviolet–visible spectroscopy detection. We present a seedless synthesis strategy for AuNSs that has the…
View article: Challenges and Opportunities for Biological Mass Spectrometry Core Facilities in the Developing World
Challenges and Opportunities for Biological Mass Spectrometry Core Facilities in the Developing World Open
The developing world is seeing rapid growth in the availability of biological mass spectrometry (MS), particularly through core facilities. As proteomics and metabolomics becomes locally feasible for investigators in these nations, applica…