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View article: Physical, chemical, and structural properties of human gastric organoid-derived mucus
Physical, chemical, and structural properties of human gastric organoid-derived mucus Open
The gastric mucus layer protects the epithelium from gastric acid and ingested pathogens. However, studies of human gastric mucus have been limited due to poor accessibility of native human mucus and the abundance of contaminants in these …
View article: Strain- and vaccine-specific effects of serum antibodies in the protection of intestinal SARS-CoV-2 infection
Strain- and vaccine-specific effects of serum antibodies in the protection of intestinal SARS-CoV-2 infection Open
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains a public health challenge worldwide. The gastrointestinal tract has emerged as an important site of infection and has been implicated as a reservoir …
View article: Antiviral responses in a Jamaican fruit bat intestinal organoid model of SARS-CoV-2 infection
Antiviral responses in a Jamaican fruit bat intestinal organoid model of SARS-CoV-2 infection Open
Bats are natural reservoirs for several zoonotic viruses, potentially due to an enhanced capacity to control viral infection. However, the mechanisms of antiviral responses in bats are poorly defined. Here we established a Jamaican fruit b…
View article: 485 Modeling gastric mucus layer physiology using human organoids
485 Modeling gastric mucus layer physiology using human organoids Open
OBJECTIVES/GOALS: Our goal is to explore the extent to which organoids can serve as models for the protective mechanisms of the stomach–the mucus barrier and the pH gradient across it. We aim to first optimize and validate an organoid-base…
View article: Antiviral response mechanisms in a Jamaican Fruit Bat intestinal organoid model of SARS-CoV-2 infection
Antiviral response mechanisms in a Jamaican Fruit Bat intestinal organoid model of SARS-CoV-2 infection Open
View article: A Synthetic Hydrogel, VitroGel® ORGANOID-3, Improves Immune Cell-Epithelial Interactions in a Tissue Chip Co-Culture Model of Human Gastric Organoids and Dendritic Cells
A Synthetic Hydrogel, VitroGel® ORGANOID-3, Improves Immune Cell-Epithelial Interactions in a Tissue Chip Co-Culture Model of Human Gastric Organoids and Dendritic Cells Open
Immunosurveillance of the gastrointestinal epithelium by mononuclear phagocytes (MNPs) is essential for maintaining gut health. However, studying the complex interplay between the human gastrointestinal epithelium and MNPs such as dendriti…
View article: Gastrointestinal organoid structure and transport
Gastrointestinal organoid structure and transport Open
Organoids are three-dimensional (3D) self-assembled, mammalian tissue cultures derived from stem cells that differentiate to contain multiple cell types. These cells spatially organize within the 3D structure and are capable of recapitulat…
View article: Fr135 DEVELOPING AN ORGANOID MODEL TO UNDERSTAND THE BAT GASTROINTESTINAL EPITHELIAL RESPONSE TO THE SEVER ACUTE RESPIRATORY CORONA VIRUS-2 (SARS-COV-2)
Fr135 DEVELOPING AN ORGANOID MODEL TO UNDERSTAND THE BAT GASTROINTESTINAL EPITHELIAL RESPONSE TO THE SEVER ACUTE RESPIRATORY CORONA VIRUS-2 (SARS-COV-2) Open
View article: A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium
A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium Open
View article: Long-term flow through human intestinal organoids with the gut organoid flow chip (GOFlowChip)
Long-term flow through human intestinal organoids with the gut organoid flow chip (GOFlowChip) Open
Human intestinal organoids (HIOs) have many research applications but are structurally limited with closed epithelial surfaces that limit material transport. Here, we present a fluidic device designed to establish long-term flow through HI…
View article: CD103 (αE Integrin) Undergoes Endosomal Trafficking in Human Dendritic Cells, but Does Not Mediate Epithelial Adhesion
CD103 (αE Integrin) Undergoes Endosomal Trafficking in Human Dendritic Cells, but Does Not Mediate Epithelial Adhesion Open
Dendritic cell (DC) expression of CD103, the α subunit of αEβ7 integrin, is thought to enable DC interactions with E-cadherin-expressing gastrointestinal epithelia for improved mucosal immunosurveillance. In the stomach, efficient DC surve…
View article: Live imaging analysis of human gastric epithelial spheroids reveals spontaneous rupture, rotation and fusion events
Live imaging analysis of human gastric epithelial spheroids reveals spontaneous rupture, rotation and fusion events Open
View article: Structuring Human Intestinal Organoids
Structuring Human Intestinal Organoids Open