Baron Chanda
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View article: Towards a unified gating scheme for the CNBD ion channel family
Towards a unified gating scheme for the CNBD ion channel family Open
Cyclic nucleotide-binding domain (CNBD) channels are critical components of numerous bioelectrical processes, including cardiac pacemaking, neuronal signaling, phototransduction in the eye, and stomatal regulation in plants. While members …
View article: A propofol binding site in the voltage sensor domain mediates inhibition of HCN1 channel activity
A propofol binding site in the voltage sensor domain mediates inhibition of HCN1 channel activity Open
Hyperpolarization-activated and cyclic nucleotide–gated (HCN) ion channels are members of the cyclic nucleotide–binding family and are crucial for regulating cellular automaticity in many excitable cells. HCN channel activation contributes…
View article: Lipid bilayers determine allostery but not intrinsic affinity of cAMP binding to pacemaker channels
Lipid bilayers determine allostery but not intrinsic affinity of cAMP binding to pacemaker channels Open
Cyclic adenosine monophosphate (cAMP), a second messenger, binds to hyperpolarization and cyclic nucleotide-gated (HCN) ion channels and regulates the automaticity of pacemaking activity. While cellular studies suggest that cAMP binding to…
View article: DeFrND: detergent-free reconstitution into native nanodiscs with designer membrane scaffold peptides
DeFrND: detergent-free reconstitution into native nanodiscs with designer membrane scaffold peptides Open
Membrane scaffold proteins-based nanodiscs (NDs) have facilitated unprecedented structural and biophysical analysis of membrane proteins in a near-native lipid environment. However, successful reconstitution of membrane proteins in NDs req…
View article: Caveolar Compartmentalization of Pacemaker Signaling is Required for Stable Rhythmicity of Sinus Nodal Cells and is Disrupted in Heart Failure
Caveolar Compartmentalization of Pacemaker Signaling is Required for Stable Rhythmicity of Sinus Nodal Cells and is Disrupted in Heart Failure Open
Background Heart rhythm relies on complex interactions between electrogenic membrane proteins and intracellular Ca 2+ signaling in sinoatrial node (SAN) myocytes; however, mechanisms underlying the functional organization of proteins invol…
View article: Structural Basis for Hyperpolarization-dependent Opening of the Human HCN1 Channel
Structural Basis for Hyperpolarization-dependent Opening of the Human HCN1 Channel Open
Hyperpolarization and cyclic-nucleotide (HCN) activated ion channels play a critical role in generating self-propagating action potentials in pacemaking and rhythmic electrical circuits in the human body. Unlike most voltage-gated ion chan…
View article: Interplay between VSD, pore, and membrane lipids in electromechanical coupling in HCN channels
Interplay between VSD, pore, and membrane lipids in electromechanical coupling in HCN channels Open
Hyperpolarized-activated and cyclic nucleotide-gated (HCN) channels are the only members of the voltage-gated ion channel superfamily in mammals that open upon hyperpolarization, conferring them pacemaker properties that are instrumental f…
View article: Author response: Interplay between VSD, pore, and membrane lipids in electromechanical coupling in HCN channels
Author response: Interplay between VSD, pore, and membrane lipids in electromechanical coupling in HCN channels Open
Full text Figures and data Side by side Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Hyperpo…
View article: Interplay between VSD, pore and membrane lipids in electromechanical coupling in HCN channels
Interplay between VSD, pore and membrane lipids in electromechanical coupling in HCN channels Open
MD simulation of human HCN1 channel: Equilibrium MD were performed for the resting, intermediate and activated states. The channel remained closed in these MD simulations. Activating conditions (usage of a negative electric field) were use…
View article: Strategies for Overcoming the Single-Molecule Concentration Barrier
Strategies for Overcoming the Single-Molecule Concentration Barrier Open
Fluorescence-based single-molecule approaches have helped revolutionize our understanding of chemical and biological mechanisms. Unfortunately, these methods are only suitable at low concentrations of fluorescent molecules so that single f…
View article: About hysteresis in Shaker: Response to note by Villalba-Galea
About hysteresis in Shaker: Response to note by Villalba-Galea Open
In this issue, Villalba-Galea (2023. J. Gen. Physiol.https://doi.org/10.1085/jgp.202313371) expresses interest in our recently published work (Cowgill and Chanda. 2023. J. Gen. Physiol.https://doi.org/10.1085/jgp.202112883). Our response p…
View article: Charge-voltage curves of Shaker potassium channel are not hysteretic at steady state
Charge-voltage curves of Shaker potassium channel are not hysteretic at steady state Open
Charge-voltage curves of many voltage-gated ion channels exhibit hysteresis but such curves are also a direct measure of free energy of channel gating and, hence, should be path-independent. Here, we identify conditions to measure steady-s…
View article: Charge-voltage curves of Shaker potassium channel are not hysteretic at steady state
Charge-voltage curves of Shaker potassium channel are not hysteretic at steady state Open
Charged residues in transmembrane segments of voltage-gated ion channels (VGICs) sense and respond to changes in the electric field. The movement of these gating charges underpins voltage-dependent activation and is also a direct metric of…
View article: Interplay between VSD, pore and membrane lipids in electromechanical coupling in HCN channels
Interplay between VSD, pore and membrane lipids in electromechanical coupling in HCN channels Open
Hyperpolarized-activated and Cyclic Nucleotide-gated (HCN) channels are the only members of the voltage-gated ion channel superfamily in mammals that open upon hyperpolarization, conferring them pacemaker properties that are instrumental f…
View article: Editor's evaluation: Structural dynamics determine voltage and pH gating in human voltage-gated proton channel
Editor's evaluation: Structural dynamics determine voltage and pH gating in human voltage-gated proton channel Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Voltage-gated proton (…
View article: Editor's evaluation: NBI-921352, a first-in-class, NaV1.6 selective, sodium channel inhibitor that prevents seizures in Scn8a gain-of-function mice, and wild-type mice and rats
Editor's evaluation: NBI-921352, a first-in-class, NaV1.6 selective, sodium channel inhibitor that prevents seizures in Scn8a gain-of-function mice, and wild-type mice and rats Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract NBI-921352 (formerly X…
View article: Mapping Electromechanical Coupling Pathways in Voltage-Gated Ion Channels: Challenges and the Way Forward
Mapping Electromechanical Coupling Pathways in Voltage-Gated Ion Channels: Challenges and the Way Forward Open
Inter- and intra-molecular allosteric interactions underpin regulation of activity in a variety of biological macromolecules. In the voltage-gated ion channel superfamily, the conformational state of the voltage-sensing domain regulates th…
View article: Mapping temperature-dependent conformational change in the voltage-sensing domain of an engineered heat-activated K <sup>+</sup> channel
Mapping temperature-dependent conformational change in the voltage-sensing domain of an engineered heat-activated K <sup>+</sup> channel Open
Significance Our understanding of fundamental biophysical mechanisms that underlie temperature-dependent gating remains limited despite the elucidation of structures of thermosensitive ion channels. According to current thinking, thermosen…
View article: Activation of the archaeal ion channel MthK is exquisitely regulated by temperature
Activation of the archaeal ion channel MthK is exquisitely regulated by temperature Open
Physiological response to thermal stimuli in mammals is mediated by a structurally diverse class of ion channels, many of which exhibit polymodal behavior. To probe the diversity of biophysical mechanisms of temperature-sensitivity, we cha…
View article: Top-down machine learning approach for high-throughput single-molecule analysis
Top-down machine learning approach for high-throughput single-molecule analysis Open
Single-molecule approaches provide enormous insight into the dynamics of biomolecules, but adequately sampling distributions of states and events often requires extensive sampling. Although emerging experimental techniques can generate suc…