Explanipedia

Flow Cytometry: Advances, Challenges and Trends Open

J. Paul Robinson, Grzegorz B. Gmyrek, Bartek Rajwa · 2025

Flow cytometry is a versatile analytical technology for measuring physical and molecular characteristics of individual cells or particles in suspension. The technology has had its greatest impact in immunology, enabling the identification …

Continuous UV-C Treatment for Safe Disposal of Pathogen Waste in Flow Cytometry Open

Sharath Narayana Iyengar, B. Corman, Bartek Rajwa, Euiwon Bae, J. Paul Robinson · 2025

The application of flow cytometry for sorting and analyzing pathogenic organisms poses significant challenges, not only due to the difficulty of maintaining a sterile environment but, more critically, because of improper handling and dispo…

Comparison of mass spectrometry and fourier transform infrared spectroscopy of plasma samples in identification of patients with fracture-related infections Open

Sarah Malek, Roman M. Natoli, Bartek Rajwa · 2025

Objectives Fracture-related infections (FRIs) have significant impact on patient outcomes. Diagnosing FRIs is challenging due to lack of robust, minimally invasive diagnostic tests in the early stages of the disease. The objective of this …

An optimal transport-based low-dimensional visualization framework for high-parameter flow cytometry Open

Abida Sanjana Shemonti, Grzegorz B. Gmyrek, Katrien Quintelier, Sofie Van Gassen, Yvan Saeys , et al. · 2025

Conventional data visualization techniques in single-cell analysis (such as two-dimensional dot plots, SPADE, PCA, t-SNE, or UMAP) often fall short in providing an intuitive understanding of high-parameter flow cytometry data. These method…

Enhancing Sensitivity of Commercial Gold Nanoparticle-Based Lateral Flow Assays: A Comparative Study of Colorimetric and Photothermal Approaches Open

Jully Blackshare, Hansel A. Mina, Amanda J. Deering, Bartek Rajwa, J. Paul Robinson , et al. · 2025

Lateral flow assays (LFAs) are extensively utilized in point-of-care diagnostics due to their affordability, simplicity, and rapid time-to-results. However, their low sensitivity remains a significant limitation, particularly for detecting…

A versatile information retrieval framework for evaluating profile strength and similarity Open

Alexandr A. Kalinin, John Arévalo, Erik Serrano, Loan Vulliard, Hillary Tsang , et al. · 2025

Gut microbiome-driven regulation of sex hormone homeostasis: a potential neuroendocrine connection Open

Anna Clapp Organski, Bartek Rajwa, Anjali Reddivari, Joan S. Jorgensen, Tzu-Wen Cross · 2025

The gut microbiome is known to have a bidirectional relationship with sex hormone homeostasis; however, its role in mediating interactions between the primary regulatory axes of sex hormones and their productions is yet to be fully underst…

Sorting and Single-Cell Deposition of Pathogenic Organisms Using Bigfoot Cell Sorter Open

Sharath Tippur Narayana Iyengar, Valery Patsekin, Euiwon Bae, Bartek Rajwa, Brianna Dowden , et al. · 2025

Accelerating antiviral drug discovery: early hazard detection with a dual zebrafish and cell culture screen of a 403 compound library Open

Lisa Truong, Andrew A. Bieberich, Raymond O. Fatig, Bartek Rajwa, Michael T. Simonich , et al. · 2024

Identifying antibiotic-resistant strains via cell sorting and elastic-light-scatter phenotyping Open

Sharath Narayana Iyengar, Brianna Dowden, Kathy Ragheb, Valery Patsekin, Bartek Rajwa , et al. · 2024

The proliferation and dissemination of antimicrobial-resistant bacteria is an increasingly global challenge and is attributed mainly to the excessive or improper use of antibiotics. Currently, the gold-standard phenotypic methodology for d…

Measuring autofluorescence spectral signatures for detecting antibiotic-resistant bacteria using Thermofisher’s Bigfoot spectral flow cytometer Open

Sharath Narayana Iyengar, Valery Patsekin, Bartek Rajwa, Euiwon Bae, Brianna Dowden , et al. · 2024

Application of flow cytometry to microbiology has been limited due to inadequate availability of bacterial-specific stains, expensive antibody-based fluorophores, ineffective stain cell permeability and challenges in differentiating bacter…

Identification of predictive patient characteristics for assessing the probability of COVID-19 in-hospital mortality Open

Bartek Rajwa, Md Mobasshir Arshed Naved, Mohammad Adibuzzaman, Ananth Grama, Babar Khan , et al. · 2024

As the world emerges from the COVID-19 pandemic, there is an urgent need to understand patient factors that may be used to predict the occurrence of severe cases and patient mortality. Approximately 20% of SARS-CoV-2 infections lead to acu…

A versatile information retrieval framework for evaluating profile strength and similarity Open

Alexandr A. Kalinin, John Arévalo, Loan Vulliard, Erik Serrano, Hillary Tsang , et al. · 2024

In profiling assays, thousands of biological properties are measured across many samples, yielding biological discoveries by capturing the state of a cell population, often at the single-cell level. However, for profiling datasets, it has …

Comparative analysis between zebrafish and an automated live-cell assay to classify developmental neurotoxicant chemicals Open

Lindsey St. Mary, Lisa Truong, Andrew A. Bieberich, Raymond O. Fatig, Bartek Rajwa , et al. · 2023

Modern toxicology's throughput has dramatically increased due to alternative models, laboratory automation, and machine learning. This has enabled comparative studies across species and assays to prioritize chemical hazard potential and to…

Hybrid Raman and Laser-Induced Breakdown Spectroscopy for Food Authentication Applications Open

Sung-Ho Shin, Iyll‐Joon Doh, Kennedy Omondi Okeyo, Euiwon Bae, J. Paul Robinson , et al. · 2023

The issue of food fraud has become a significant global concern as it affects both the quality and safety of food products, ultimately resulting in the loss of customer trust and brand loyalty. To address this problem, we have developed an…

Identification of predictive patient characteristics for assessing the probability of COVID-19 in-hospital mortality Open

Bartek Rajwa, Md Mobasshir Arshed Naved, Mohammad Adibuzzaman, Ananth Grama, Babar Khan , et al. · 2023

As the world emerges from the COVID-19 pandemic, there is an urgent need to understand patient factors that may be used to predict the occurrence of severe cases and patient mortality. Approximately 20% of SARS-CoV-2 infections lead to acu…

Flow Cytometry: The Next Revolution Open

J. Paul Robinson, Raluca Ostafe, Sharath Narayana Iyengar, Bartek Rajwa, Rainer Fischer · 2023

Unmasking the subtleties of the immune system requires both a comprehensive knowledge base and the ability to interrogate that system with intimate sensitivity. That task, to a considerable extent, has been handled by an iterative expansio…

Bacterial Colony Phenotyping with Hyperspectral Elastic Light Scattering Patterns Open

Iyll‐Joon Doh, Diana Vanessa Sarria Zuniga, Sungho Shin, Robert E. Pruitt, Bartek Rajwa , et al. · 2023

The elastic light-scatter (ELS) technique, which detects and discriminates microbial organisms based on the light-scatter pattern of their colonies, has demonstrated excellent classification accuracy in pathogen screening tasks. The implem…

A novel statistical methodology for quantifying the spatial arrangements of axons in peripheral nerves Open

Abida Sanjana Shemonti, Emanuele Plebani, Natália P. Biscola, Deborah Jaffey, Leif A. Havton , et al. · 2023

A thorough understanding of the neuroanatomy of peripheral nerves is required for a better insight into their function and the development of neuromodulation tools and strategies. In biophysical modeling, it is commonly assumed that the co…

Surface Environment and Energy Density Effects on the Detection and Disinfection of Microorganisms Using a Portable Instrument Open

Sungho Shin, Brianna Dowden, Iyll‐Joon Doh, Bartek Rajwa, Euiwon Bae , et al. · 2023

Real-time detection and disinfection of foodborne pathogens are important for preventing foodborne outbreaks and for maintaining a safe environment for consumers. There are numerous methods for the disinfection of hazardous organisms, incl…

Rapid Food Authentication Using a Portable Laser-Induced Breakdown Spectroscopy System Open

Xi Wu, Sung-Ho Shin, Carmen Gondhalekar, Valery Patsekin, Euiwon Bae , et al. · 2023

Laser-induced breakdown spectroscopy (LIBS) is an atomic-emission spectroscopy technique that employs a focused laser beam to produce microplasma. Although LIBS was designed for applications in the field of materials science, it has lately…