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View article: Automated Imaging and Analysis of Platelet, Coagulation and Fibrinolysis Activities Using a Novel Flow Chip-Based System at Physiological Temperature
Automated Imaging and Analysis of Platelet, Coagulation and Fibrinolysis Activities Using a Novel Flow Chip-Based System at Physiological Temperature Open
Conventional whole-blood flow assays for quantifying thrombus formation are typically performed at room temperature and are technically demanding, which limits their translational applicability. We engineered a novel, disposable, mountable…
View article: Plasminogen activation and plasmin activity are not necessary to prevent venous thrombosis/thromboembolism
Plasminogen activation and plasmin activity are not necessary to prevent venous thrombosis/thromboembolism Open
Suppression of plasminogen activation and/or plasmin activity (PA) reduces blood loss and decreases hemorrhage-related death. However, whether the endogenous PA system is a biological mechanism to prevent intravascular thrombus formation i…
View article: Role of inflammation and haemostasis on aetiology and prognosis in young patients with ischaemic stroke: study protocol of the Observational Dutch Young Symptomatic StrokE study—EXTended (ODYSSEY-nEXT) – a multicentre prospective cohort study
Role of inflammation and haemostasis on aetiology and prognosis in young patients with ischaemic stroke: study protocol of the Observational Dutch Young Symptomatic StrokE study—EXTended (ODYSSEY-nEXT) – a multicentre prospective cohort study Open
Introduction The cause of ischaemic stroke at a young age remains unknown in 30% of cases, highlighting the need to identify hidden causes and risk factors in young patients. Transient and chronic risk factors may interact with the inflamm…
View article: Age- and sex-dependency of thrombin generation parameters in the general Italian population: the Moli-sani study
Age- and sex-dependency of thrombin generation parameters in the general Italian population: the Moli-sani study Open
Background Recent developments have made the thrombin generation (TG) test accessible to the clinical laboratory. Therefore, the clinical interpretation of TG parameters has become of increasing interest, and reference values are required.…
View article: High Prevalence of Acquired Platelet Secretion Defects in Multiple Myeloma
High Prevalence of Acquired Platelet Secretion Defects in Multiple Myeloma Open
Thrombocytopenia at admission predicts mortality in multiple myeloma (MM) and might link to disease progression. Although thrombocytopenia is known to be associated with MM, a possible thrombopathy is clinically less known. We conducted a …
View article: Interpreting high levels of unfolded Von Willebrand Factor in patients with the antiphospholipid syndrome
Interpreting high levels of unfolded Von Willebrand Factor in patients with the antiphospholipid syndrome Open
Introduction Unfolded Von Willebrand Factor (VWF) is increased in thrombotic pathologies such as myocardial infarction. Unfolded VWF mediates the binding of platelets without the need for collagen. β 2 -glycoprotein I (β 2 -GPI) is a natur…
View article: Thrombomodulin is a stronger indicator of combined oral contraceptives-induced activated protein C pathway resistance in the thrombin generation test than activated protein C
Thrombomodulin is a stronger indicator of combined oral contraceptives-induced activated protein C pathway resistance in the thrombin generation test than activated protein C Open
Background The mechanism by which combined oral contraceptives (COCs) lead to hypercoagulation is not fully understood, although activated protein C (APC) pathway resistance has been implicated. APC and thrombomodulin (TM) tend to be consi…
View article: Plasmin generation analysis in patients with bleeding disorder of unknown cause
Plasmin generation analysis in patients with bleeding disorder of unknown cause Open
Bleeding disorder of unknown cause (BDUC) is a diagnosis of exclusion after evaluation of plasma coagulation and platelet function. Patients with BDUC (n = 375) recorded in the Vienna Bleeding Biobank were analyzed in comparison with healt…
View article: COVID-19 and Sepsis Are Associated With Different Abnormalities in Plasma Procoagulant and Fibrinolytic Activity
COVID-19 and Sepsis Are Associated With Different Abnormalities in Plasma Procoagulant and Fibrinolytic Activity Open
OBJECTIVE: Coronavirus disease 2019 (COVID-19) is associated with derangement in biomarkers of coagulation and endothelial function and has been likened to the coagulopathy of sepsis. However, clinical laboratory metrics suggest key differ…
View article: Platelet Activation Pathways Controlling Reversible Integrin αIIbβ3 Activation
Platelet Activation Pathways Controlling Reversible Integrin αIIbβ3 Activation Open
Background Agonist-induced platelet activation, with the integrin αIIbβ3 conformational change, is required for fibrinogen binding. This is considered reversible under specific conditions, allowing a second phase of platelet aggregation. T…
View article: Lipid nanoparticles and siRNA targeting plasminogen provide lasting inhibition of fibrinolysis in mouse and dog models of hemophilia A
Lipid nanoparticles and siRNA targeting plasminogen provide lasting inhibition of fibrinolysis in mouse and dog models of hemophilia A Open
Antifibrinolytic drugs are used extensively for on-demand treatment of severe acute bleeding. Controlling fibrinolysis may also be an effective strategy to prevent or lessen chronic recurring bleeding in bleeding disorders such as hemophil…
View article: A thrombin-driven neural net diagnoses the antiphospholipid syndrome without the need for interruption of anticoagulation
A thrombin-driven neural net diagnoses the antiphospholipid syndrome without the need for interruption of anticoagulation Open
Thrombosis is an important manifestation of the antiphospholipid syndrome (APS). The thrombin generation (TG) test is a global hemostasis assay, and increased TG is associated with thrombosis. APS is currently diagnosed based on clinical a…
View article: Plasminogen deficiency suppresses pancreatic ductal adenocarcinoma disease progression
Plasminogen deficiency suppresses pancreatic ductal adenocarcinoma disease progression Open
Pancreatic ductal adenocarcinoma (PDAC) is a highly fatal metastatic disease associated with robust activation of the coagulation and fibrinolytic systems. However, the potential contribution of the primary fibrinolytic protease plasminoge…
View article: Cellular Components in Whole Blood Contribute to the Development of Thromboembolic Events in Patients with Pancreatic Cancer
Cellular Components in Whole Blood Contribute to the Development of Thromboembolic Events in Patients with Pancreatic Cancer Open
Introduction Thromboembolic disease is a major complication in patients with pancreatic ductal adenocarcinoma (PDAC), causing morbidity and mortality. One in five patients with newly diagnosed PDAC develops a venous thromboembolism (VTE), …
View article: Periprocedural continuation versus interruption of oral anticoagulant drugs during transcatheter aortic valve implantation: rationale and design of the POPular PAUSE TAVI trial
Periprocedural continuation versus interruption of oral anticoagulant drugs during transcatheter aortic valve implantation: rationale and design of the POPular PAUSE TAVI trial Open
About one-third of patients undergoing transcatheter aortic valve implantation (TAVI) use oral anticoagulants (OAC), mainly due to atrial fibrillation. General guidelines advise interrupting OAC in patients with a high risk of bleeding und…
View article: OC 09.1 Epitope Specificity of Anti-Prothrombin Antibodies that Express Lupus Anticoagulant Activity
OC 09.1 Epitope Specificity of Anti-Prothrombin Antibodies that Express Lupus Anticoagulant Activity Open
Background: Sickle cell disease (SCD) is characterized by endothelial dysfunction that drives thrombin generation, inflammation, and vasoocclusion.We have shown that endothelial protease activated receptor 1 (PAR1) has both detrimental and…
View article: OC 45.4 Crucial Roles of Red Blood Cells and Platelets in Coagulation
OC 45.4 Crucial Roles of Red Blood Cells and Platelets in Coagulation Open
citH3; Cell Death Detection ELISAPlus Roche, modified), DNA-histone complexes (Cell Death Detection ELISAPlus) and calprotectin (Human Calprotectin ELISA Kit, Hycult Biotech).We performed the statistical analysis with SPSS v21.Results: PCd…
View article: PB0733 Patients with Pancreatic Cancer have a Disturbed Whole Blood Thrombin Generation Profile
PB0733 Patients with Pancreatic Cancer have a Disturbed Whole Blood Thrombin Generation Profile Open
Table:The inhibitory effect of Nilotinib and its analogues on P-selectin and PAC-1 expression in PRP activated by AA, studied by Flow cytometry. Conclusion(s):All compounds exhibited more potent antiplatelet activity, compared to Nilotinib…