Bashu Dev Pardhe
YOU?
Author Swipe
View article: CAZyme analysis and functional characterization of a new GH18 chitin hydrolase from Gelidibacter salicanalis PAMC21136
CAZyme analysis and functional characterization of a new GH18 chitin hydrolase from Gelidibacter salicanalis PAMC21136 Open
Gelidibacter salicanalis strains remain poorly understood in terms of their genomic attributes and are rarely reported for the degradation of polysaccharides within the niche. Gelidibacter salicanalis PAMC21136, an Antarctic isolate, was s…
View article: Analysis of critical residues for peroxygenation and improved peroxygenase activity via in situ H2O2 generation in CYP105D18
Analysis of critical residues for peroxygenation and improved peroxygenase activity via in situ H2O2 generation in CYP105D18 Open
Limited numbers of CYPs have been reported to work naturally as peroxygenases. The peroxide shunt pathway can be efficiently used as an alternative for the NAD(P)H and reductase systems, particularly in high hydrogen peroxide (H 2 O 2 ) re…
View article: Identification and evaluation of CAZyme genes, along with functional characterization of a new GH46 chitosanase from Streptomyces sp. KCCM12257
Identification and evaluation of CAZyme genes, along with functional characterization of a new GH46 chitosanase from Streptomyces sp. KCCM12257 Open
The genomic analysis of Streptomyces sp. KCCM12257 presented 233 CAZyme genes with a predominant glycosyl hydrolase family. This contributes degradation of various polysaccharides including chitin and chitosan, and other promising candidat…
View article: Biochemical and structural basis of mercuric reductase, GbsMerA, from Gelidibacter salicanalis PAMC21136
Biochemical and structural basis of mercuric reductase, GbsMerA, from Gelidibacter salicanalis PAMC21136 Open
Heavy metals, including mercury, are non-biodegradable and highly toxic to microorganisms even at low concentrations. Understanding the mechanisms underlying the environmental adaptability of microorganisms with Hg resistance holds promise…
View article: H <sub>2</sub> O <sub>2</sub> -Driven Hydroxylation of Steroids Catalyzed by Cytochrome P450 CYP105D18: Exploration of the Substrate Access Channel
H <sub>2</sub> O <sub>2</sub> -Driven Hydroxylation of Steroids Catalyzed by Cytochrome P450 CYP105D18: Exploration of the Substrate Access Channel Open
The structural modification of steroids is a challenging chemical reaction. Modifying the core ring and the side chain improves the biological activity of steroids.
View article: Implication of Salivary Biochemical Parameters for Diagnosis and Prognosis of Type 2 Diabetes Mellitus
Implication of Salivary Biochemical Parameters for Diagnosis and Prognosis of Type 2 Diabetes Mellitus Open
Background. Clinical laboratory diagnosis and prognosis for diabetes mellitus is performed using blood as a major specimen; however, saliva may represent as an alternative noninvasive specimen of choice. This study aims to evaluate salivar…
View article: Characterization of high-H<sub>2</sub>O<sub>2</sub>-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation
Characterization of high-H<sub>2</sub>O<sub>2</sub>-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation Open
The bacterial CYP105 family is involved in secondary metabolite biosynthetic pathways and plays essential roles in the biotransformation of xenobiotics. This study investigates the newly identified H 2 O 2 -mediated CYP105D18 from Streptom…
View article: Vitamin D deficiency and cardiovascular risk in type 2 diabetes population
Vitamin D deficiency and cardiovascular risk in type 2 diabetes population Open
This study aims to assess vitamin D deficiency-induced dyslipidemia and cardiovascular disease (CVD) risk in poor glycemic control among type 2 diabetes mellitus (T2DM) patients. This study was carried out among 455 T2DM patients involving…
View article: <p>Metabolic Syndrome and Diabetes Risk Among Young Adult Students in the Health Sciences from Kathmandu, Nepal</p>
Metabolic Syndrome and Diabetes Risk Among Young Adult Students in the Health Sciences from Kathmandu, Nepal Open
Metabolic syndrome is present, although in low numbers in young adults putting them at risk to develop diabetes in the near future. Early assessment of metabolic syndrome and diabetes risk in young may provide insights for preventive and c…
View article: <p>Classical Dermatomyositis: A Case Report</p>
Classical Dermatomyositis: A Case Report Open
Sushant Pokhrel,1,2 Bashu Dev Pardhe,1,3 Nisha Giri,2 Rakesh Pokhrel,2,4 Deliya Paudel2,5 1Department of Laboratory Medicine, Manmohan Memorial Institute of Health Sciences, Kathmandu, Nepal; 2Modern Diagnostic Laboratory and Research Cent…
View article: <p>Self-Care Adherence And Barriers To Good Glycaemic Control In Nepalese Type 2 Diabetes Mellitus Patients: A Hospital-Based Cross-Sectional Study</p>
Self-Care Adherence And Barriers To Good Glycaemic Control In Nepalese Type 2 Diabetes Mellitus Patients: A Hospital-Based Cross-Sectional Study Open
The poor glycaemic control was associated with poor adherence to self-care adherence and their barriers in our diabetic population. Integrated knowledge on diabetes management should be targeted to improve glycaemic control in our communit…
View article: Elevated liver transaminases and their association with metabolic syndrome in type 2 diabetic patients attending tertiary care hospital of Nepal
Elevated liver transaminases and their association with metabolic syndrome in type 2 diabetic patients attending tertiary care hospital of Nepal Open
Background: Worldwide, the prevalence of the metabolic syndrome (MetS) is estimated to be 70% among those with type 2 diabetes mellitus (T2DM). T2DM and MetS are associated with abnormal liver enzyme levels, which can be the result of non-…
View article: Effect of age and estrogen on biochemical markers of bone turnover in postmenopausal women: a population-based study from Nepal
Effect of age and estrogen on biochemical markers of bone turnover in postmenopausal women: a population-based study from Nepal Open
Timely diagnosis of osteoporosis in women would be of significant benefit for effective care for required populations and help to minimize mortality rate and financial burden of our country.
View article: Elevated serum uric acid and triglycerides level in the patients with Type II Diabetes Mellitus- a Nepalese case control study
Elevated serum uric acid and triglycerides level in the patients with Type II Diabetes Mellitus- a Nepalese case control study Open
Background: Hyperuricemia is associated with type 2 diabetes, which is a metabolic disorder of multiple etiologies resulting from defects in insulin action. The present study wascarried out to look for any association between uric acid and…
View article: Elevated Cardiovascular Risks among Postmenopausal Women: A Community Based Case Control Study from Nepal
Elevated Cardiovascular Risks among Postmenopausal Women: A Community Based Case Control Study from Nepal Open
Cardiovascular disease (CVD) is one of the leading causes of death worldwide which is more prevalent in women after menopause. Hormonal changes associated with menopause are accountable for dyslipidemic pattern that causes CVD and associat…
View article: Shigellosis Caused by CTX-M Type ESBL Producing<i> Shigella flexneri</i> in Two Siblings of Rural Nepal: First Case Report from the Country
Shigellosis Caused by CTX-M Type ESBL Producing<i> Shigella flexneri</i> in Two Siblings of Rural Nepal: First Case Report from the Country Open
Shigellosis is an acute infectious disease characterized as severe bloody diarrhea (dysentery) and is accountable for a significant burden of morbidity and mortality especially in children under the age of 5 years. Antimicrobial therapy is…