Behrokh Farahmand
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View article: World Flu Day 2024 with One Health Approach in Iran
World Flu Day 2024 with One Health Approach in Iran Open
Introduction: The global impact and significance of influenza are profound, from the 1918 Spanish flu pandemic to recent seasonal and avian influenza outbreaks. The ongoing need for international collaboration to control and mitigate the e…
View article: Developing of SARS-CoV-2 fusion protein expressed in E. coli Shuffle T7 for enhanced ELISA detection sensitivity – an integrated experimental and bioinformatic approach
Developing of SARS-CoV-2 fusion protein expressed in E. coli Shuffle T7 for enhanced ELISA detection sensitivity – an integrated experimental and bioinformatic approach Open
In the recent COVID-19 pandemic, developing effective diagnostic assays is crucial for controlling the spread of the SARS-CoV-2 virus. Multi-domain fusion proteins are a promising approach to detecting SARS-CoV-2 antibodies. In this study,…
View article: Evaluation of PastoCovac plus vaccine as a booster dose on vaccinated individuals with inactivated COVID-19 vaccine
Evaluation of PastoCovac plus vaccine as a booster dose on vaccinated individuals with inactivated COVID-19 vaccine Open
COVID-19 pandemic has been managed through global vaccination programs. However, the antibody waning in various types of vaccines came to notice. Hereby, PastoCovac Plus as a protein subunit vaccine was investigated in immunized health car…
View article: Phylogenetic analysis and docking study of neuraminidase gene of influenza A/H1N1 viruses circulating in Iran from 2010 to 2019
Phylogenetic analysis and docking study of neuraminidase gene of influenza A/H1N1 viruses circulating in Iran from 2010 to 2019 Open
Influenza A viruses (H1N1) have been consistently one of the most evolving viruses that escape from vaccine-induced immunity. Although there has been a rapid rise in human influenza virus knowledge since the 2009 pandemic, the molecular in…
View article: Expression and Purification of SARS CoV-2 RBD Protein in the Prokaryotic Expression System to Evaluate the IgG Antibody in the Serums of COVID-19 Recovered Cases
Expression and Purification of SARS CoV-2 RBD Protein in the Prokaryotic Expression System to Evaluate the IgG Antibody in the Serums of COVID-19 Recovered Cases Open
زمینه و هدف یکی از مهمترین پروتئینهای ویروس 2-COV–SARS پروتئین S است که از دو زیرواحد 1S و 2S تشکیل شدهاست. مهمترین ناحیه در زیرواحد S1 ناحیه اتصال به رسپتور ) RBD ( است که نقش کلیدی در اتصال به رسپتورهای ACE راایفا میکند. RBD یک ناحیه حفا…
View article: Computational peptide engineering approach for selection of the new C05 antibody-driven peptide with potency to blocking influenza a virus attachment; from <i>in silico</i> to <i>in vivo</i>
Computational peptide engineering approach for selection of the new C05 antibody-driven peptide with potency to blocking influenza a virus attachment; from <i>in silico</i> to <i>in vivo</i> Open
Antiviral drugs are currently used to prevent or treat viral infections like influenza A Virus (IAV). Nonetheless, annual genetic mutations of influenza viruses make them resistant to efficient treatment by current medications. Antiviral p…
View article: Fusion Protein Consisting of Hemagglutinin Small Subunit and Truncated Nucleoprotein as a Universal Influenza Vaccine Candidate: Starting In-Silico Evaluation Toward In Vitro Expression
Fusion Protein Consisting of Hemagglutinin Small Subunit and Truncated Nucleoprotein as a Universal Influenza Vaccine Candidate: Starting In-Silico Evaluation Toward In Vitro Expression Open
Background: Influenza virus is a respiratory pathogen, which causes high degree of mortality and morbidity during seasonal epidemics and sporadic pandemics. By selecting conserved antigenic proteins, for example, hemagglutinin small subuni…
View article: In Vivo Evaluation of the Adjuvant-Free Influenza Nucleoprotein Vaccine
In Vivo Evaluation of the Adjuvant-Free Influenza Nucleoprotein Vaccine Open
Background: The influenza virus's Nucleoprotein (NP) is a highly conserved protein used in vaccine design. Alum, besides its neurotoxicity, is a commonly used adjuvant. Objectives: We tested the NP's efficacy as an alum-free flu vaccine. M…
View article: Evaluation of immunogenicity of recombinant influenza nucleoprotein (NP) for universal vaccine
Evaluation of immunogenicity of recombinant influenza nucleoprotein (NP) for universal vaccine Open
Background: Influenza vaccines based on conserved proteins are being developed persistently. The conserved protein vaccines based on Nucleoprotein (NP) are highly protected vaccines against influenza viruses that can be used as a Universal…
View article: Computational drug repurposing study of antiviral drugs against main protease, RNA polymerase, and spike proteins of SARS-CoV-2 using molecular docking method
Computational drug repurposing study of antiviral drugs against main protease, RNA polymerase, and spike proteins of SARS-CoV-2 using molecular docking method Open
Objectives The new Coronavirus (SARS-CoV-2) created a pandemic in the world in late 2019 and early 2020. Unfortunately, despite the increasing prevalence of the disease, there is no effective drug for the treatment. A computational drug re…
View article: Prokaryotic Expression of the Influenza A (H5N1) Neuraminidase
Prokaryotic Expression of the Influenza A (H5N1) Neuraminidase Open
Background and purpose: Highly Pathogenic Influenza A(H5N1) viruses cause vast economic losses throughout the world. They may circulate in animals and be able to spread from human to human. Therefore, launching diagnostic tests are highly …
View article: Association of the host genetic factors, hypercholesterolemia and diabetes with mild influenza in an Iranian population
Association of the host genetic factors, hypercholesterolemia and diabetes with mild influenza in an Iranian population Open
Background Variation in host genetic factors may result in variation in the host immune response to the infection. Some chronic diseases may also affect individuals’ susceptibility to infectious diseases. The aim of this study was to evalu…
View article: Clinical Characteristics of SARS-CoV-2&nbsp;by Re-infection Vs. Reactivation: A Case Series From Iran
Clinical Characteristics of SARS-CoV-2 by Re-infection Vs. Reactivation: A Case Series From Iran Open
Purpose : COVID-19 immunity in infected individuals may not be persistent. The specific response wanes in patients who have recovered from this infection. Nevertheless, it has not been fully understood whether true re-infection occurs or t…
View article: Characterization of the Non-Structural Gene of Influenza A Virus H1N1 in Iran
Characterization of the Non-Structural Gene of Influenza A Virus H1N1 in Iran Open
A Virus H1N1 in IranBackground: The Non-Structural (NS) genomic segment of influenza A virus expresses two proteins (NS1 and NS2) responsible for virulence and pathogenicity.Here, we characterize the NS gene of H1N1 influenza viruses isola…
View article: Design of a Universal Influenza A Vaccine Candidate Based on M2e.FliC; Immunoinformatics Analysis, Protein Modeling, and Its Expression in Escherichia coli
Design of a Universal Influenza A Vaccine Candidate Based on M2e.FliC; Immunoinformatics Analysis, Protein Modeling, and Its Expression in Escherichia coli Open
Background: Due to the rapid accumulation of mutations in influenza virus and the unpredictability of new influenza, the current influenza vaccines require an almost yearly reformulation. The extracellular domain of matrix protein 2 (M2e) …
View article: In Silico Analysis and Expression of Influenza Virus 3M2e-HA2 Chimer Protein Fused to C-Terminal Domain of Leishmania major HSP70
In Silico Analysis and Expression of Influenza Virus 3M2e-HA2 Chimer Protein Fused to C-Terminal Domain of Leishmania major HSP70 Open
Background: Design and construction of a universal vaccine based on conserved influenza antigens is the best way to protect populations from unforeseen influenza outbreaks. The ectodomain of matrix protein 2 (M2e) and hemagglutinin stalk d…
View article: Association of IFITM3 rs12252 polymorphisms, BMI, diabetes, and hypercholesterolemia with mild flu in an Iranian population
Association of IFITM3 rs12252 polymorphisms, BMI, diabetes, and hypercholesterolemia with mild flu in an Iranian population Open
IR.PII.REC.1395.3.
View article: Purification of Influenza virus A (H1N1) recombinant Hemagglutinin (HA1) and polyclonal antibody production
Purification of Influenza virus A (H1N1) recombinant Hemagglutinin (HA1) and polyclonal antibody production Open
Background: Each year, Human influenza A (H1N1) virus causes moderate to severe infections with a high prevalence throughout the world. Accordingly, the rapid, sensitive and cost-effective laboratory diagnosis based on viral antigen detect…