Ben A. Evans
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View article: Stay-on-Task Exercises as a Tool To Maintain Focus during a CRISPR CURE
Stay-on-Task Exercises as a Tool To Maintain Focus during a CRISPR CURE Open
Course-based undergraduate research experiences (CURE) offer the chance for students to experience authentic research investigation in a classroom setting. Such hands-on experiences afford unique opportunities work on a semi-independent re…
View article: SpRY Cas9 Can Utilize a Variety of Protospacer Adjacent Motif Site Sequences To Edit the Candida albicans Genome
SpRY Cas9 Can Utilize a Variety of Protospacer Adjacent Motif Site Sequences To Edit the Candida albicans Genome Open
Genetic modification of the human fungal pathogen Candida albicans allows us to better understand how fungi differ from humans at the molecular level and play essential roles in the therapeutic development. CRISPR/Cas9-mediated genome edit…
View article: How CRISPR-Mediated Genome Editing is Affecting Undergraduate Biology Education
How CRISPR-Mediated Genome Editing is Affecting Undergraduate Biology Education Open
In 2010, the CRISPR/Cas system of Streptococcus thermophilus was found necessary and sufficient to cleave bacteriophage DNA. Since this time, CRISPR went from a niche scientific field to the laboratories of major research institutions, und…
View article: CRISPR-mediated Genome Editing of the Human Fungal Pathogen <em>Candida albicans</em>
CRISPR-mediated Genome Editing of the Human Fungal Pathogen <em>Candida albicans</em> Open
This method describes the efficient CRISPR mediated genome editing of the diploid human fungal pathogen Candida albicans. CRISPR-mediated genome editing in C. albicans requires Cas9, guide RNA, and repair template. A plasmid expressing a y…
View article: Restriction digest screening facilitates efficient detection of site-directed mutations introduced by CRISPR in <i>C. albicans UME6</i>
Restriction digest screening facilitates efficient detection of site-directed mutations introduced by CRISPR in <i>C. albicans UME6</i> Open
Introduction of point mutations to a gene of interest is a powerful tool when determining protein function. CRISPR-mediated genome editing allows for more efficient transfer of a desired mutation into a wide range of model organisms. Tradi…