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View article: HIV-phyloTSI: subtype-independent estimation of time since HIV-1 infection for cross-sectional measures of population incidence using deep sequence data
HIV-phyloTSI: subtype-independent estimation of time since HIV-1 infection for cross-sectional measures of population incidence using deep sequence data Open
Background Estimating the time since HIV infection (TSI) at population level is essential for tracking changes in the global HIV epidemic. Most methods for determining TSI give a binary classification of infections as recent or non-recent …
View article: HIV-2 evades restriction by ZAP through adaptations in the U3 LTR region despite increased CpG levels
HIV-2 evades restriction by ZAP through adaptations in the U3 LTR region despite increased CpG levels Open
Simian immunodeficiency viruses infecting sooty mangabeys (SIVsmm) gave rise to nine groups of human immunodeficiency virus type 2 (HIV-2). Two of these (A and B) spread substantially with an estimated 1–2 million individuals affected. The…
View article: Long-term effect of temporary ART initiated during primary HIV-1 infection on viral persistence
Long-term effect of temporary ART initiated during primary HIV-1 infection on viral persistence Open
Initiation of antiretroviral therapy (ART) during primary HIV-1 infection (PHI) has been proposed to limit the formation of HIV-1 reservoirs. However, it remains unknown whether temporary ART initiated during PHI has a long-term effect on …
View article: Pure CD32+ CD4+ Cells Are Cytotoxic Memory CD4+ T Lymphocytes Highly Enriched for HIV-1 DNA
Pure CD32+ CD4+ Cells Are Cytotoxic Memory CD4+ T Lymphocytes Highly Enriched for HIV-1 DNA Open
The elusive viral reservoir is the major obstacle to an HIV-1 cure. Cell surface protein CD32 has been proposed to pinpoint cells with a very high proviral enrichment, potentially providing a tool for selective therapeutic targeting of the…
View article: Phenotyping Viral Reservoirs to Reveal HIV-1 Hiding Places
Phenotyping Viral Reservoirs to Reveal HIV-1 Hiding Places Open
Purpose of Review Despite suppressive antiretroviral therapy (ART), HIV-1 reservoirs persist in various cell types and tissues and reignite active replication if therapy is stopped. Persistence of the viral reservoirs in people with HIV-1 …
View article: Virus Research: 40 years and still going strong
Virus Research: 40 years and still going strong Open
View article: Evaluation of the effect of RNA secondary structure on Cas13d-mediated target RNA cleavage
Evaluation of the effect of RNA secondary structure on Cas13d-mediated target RNA cleavage Open
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas13d system was adapted as a powerful tool for targeting viral RNA sequences, making it a promising approach for antiviral strategies. Understanding the influence of …
View article: Reverse transcription of HIV-1 2-LTR circle transcripts does frequently cause 3'-polypurine tract mutations implicated in dolutegravir resistance
Reverse transcription of HIV-1 2-LTR circle transcripts does frequently cause 3'-polypurine tract mutations implicated in dolutegravir resistance Open
View article: Persistent HIV-1 transcription during ART: time to reassess its significance?
Persistent HIV-1 transcription during ART: time to reassess its significance? Open
Purpose of review Despite suppressive antiretroviral therapy (ART), HIV-1 reservoirs persist and reignite viral replication if therapy is interrupted. Persistence of the viral reservoir in people with HIV-1 (PWH) is the main obstacle to an…
View article: PA-305 FC gamma receptor gene polymorphisms and reservoir size in HIV patients in Ghana
PA-305 FC gamma receptor gene polymorphisms and reservoir size in HIV patients in Ghana Open
Background Fc gamma receptors (FcγR) are cell surface glycoproteins that bind to the Fc portions of immunoglobulin IgG antibodies to elicit diverse effector functions. Polymorphisms in different FcγR genes have been associated with HIV inf…
View article: CRISPR-Cas attack of HIV-1 proviral DNA can cause unintended deletion of surrounding cellular DNA
CRISPR-Cas attack of HIV-1 proviral DNA can cause unintended deletion of surrounding cellular DNA Open
Several studies indicate that CRISPR-Cas gene-editing systems can be used to inactivate the HIV-1 proviral DNA in infected cells. Such gene editing introduces mutations, mostly small insertions and deletions (indels), at the targeted sites…
View article: In-house ELISA protocols for capsid p24 detection of diverse HIV isolates
In-house ELISA protocols for capsid p24 detection of diverse HIV isolates Open
View article: AAV vectors displaying bispecific DARPins enable dual-control targeted gene delivery
AAV vectors displaying bispecific DARPins enable dual-control targeted gene delivery Open
View article: Recent Advances Using Genetic Therapies Against Infectious Diseases and for Vaccination
Recent Advances Using Genetic Therapies Against Infectious Diseases and for Vaccination Open
The development of prophylatic or therapeutic medicines for infectious diseases is one of the priorities for health organizations worldwide. Innovative solutions are required to achieve effective, safe, and accessible treatments for most i…
View article: CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures
CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures Open
View article: HIV-1 3′-Polypurine Tract Mutations Confer Dolutegravir Resistance by Switching to an Integration-Independent Replication Mechanism via 1-LTR Circles
HIV-1 3′-Polypurine Tract Mutations Confer Dolutegravir Resistance by Switching to an Integration-Independent Replication Mechanism via 1-LTR Circles Open
The integrase inhibitor DTG is a potent inhibitor of HIV replication and is currently recommended in drug regimens for people living with HIV. Whereas HIV normally escapes from antiviral drugs by the acquisition of specific mutations in th…
View article: FcγRIIA-specific DARPins as novel tools in blood cell analysis and platelet aggregation
FcγRIIA-specific DARPins as novel tools in blood cell analysis and platelet aggregation Open
View article: Supplementary Figure 1 from Construction of a Minimal HIV-1 Variant that Selectively Replicates in Leukemic Derived T-Cell Lines: Towards a New Virotherapy Approach
Supplementary Figure 1 from Construction of a Minimal HIV-1 Variant that Selectively Replicates in Leukemic Derived T-Cell Lines: Towards a New Virotherapy Approach Open
Supplementary Figure 1 from Construction of a Minimal HIV-1 Variant that Selectively Replicates in Leukemic Derived T-Cell Lines: Towards a New Virotherapy Approach
View article: Data from Construction of a Minimal HIV-1 Variant that Selectively Replicates in Leukemic Derived T-Cell Lines: Towards a New Virotherapy Approach
Data from Construction of a Minimal HIV-1 Variant that Selectively Replicates in Leukemic Derived T-Cell Lines: Towards a New Virotherapy Approach Open
T-cell acute lymphoblastic leukemia is a high-risk type of blood-cell cancer. We analyzed the possibility of developing virotherapy for T-cell acute lymphoblastic leukemia. Virotherapy is based on the exclusive replication of a virus in le…
View article: Supplementary Figure 1 from Construction of a Minimal HIV-1 Variant that Selectively Replicates in Leukemic Derived T-Cell Lines: Towards a New Virotherapy Approach
Supplementary Figure 1 from Construction of a Minimal HIV-1 Variant that Selectively Replicates in Leukemic Derived T-Cell Lines: Towards a New Virotherapy Approach Open
Supplementary Figure 1 from Construction of a Minimal HIV-1 Variant that Selectively Replicates in Leukemic Derived T-Cell Lines: Towards a New Virotherapy Approach
View article: Data from Construction of a Minimal HIV-1 Variant that Selectively Replicates in Leukemic Derived T-Cell Lines: Towards a New Virotherapy Approach
Data from Construction of a Minimal HIV-1 Variant that Selectively Replicates in Leukemic Derived T-Cell Lines: Towards a New Virotherapy Approach Open
T-cell acute lymphoblastic leukemia is a high-risk type of blood-cell cancer. We analyzed the possibility of developing virotherapy for T-cell acute lymphoblastic leukemia. Virotherapy is based on the exclusive replication of a virus in le…
View article: Efficient CRISPR-Cas13d-Based Antiviral Strategy to Combat SARS-CoV-2
Efficient CRISPR-Cas13d-Based Antiviral Strategy to Combat SARS-CoV-2 Open
The current SARS-CoV-2 pandemic forms a major global health burden. Although protective vaccines are available, concerns remain as new virus variants continue to appear. CRISPR-based gene-editing approaches offer an attractive therapeutic …
View article: HIV persistence: silence or resistance?
HIV persistence: silence or resistance? Open
Despite decades of suppressive antiretroviral therapy, human immunodeficiency virus (HIV) reservoirs in infected individuals persist and fuel viral rebound once therapy is interrupted. The persistence of viral reservoirs is the main obstac…
View article: HIV co-opts a cellular antiviral mechanism, activation of stress kinase PKR by its RNA, to enable splicing of rev/tat mRNA
HIV co-opts a cellular antiviral mechanism, activation of stress kinase PKR by its RNA, to enable splicing of rev/tat mRNA Open
View article: A CRISPR-Cas Cure for HIV/AIDS
A CRISPR-Cas Cure for HIV/AIDS Open
Human immunodeficiency virus (HIV) infections and HIV-induced acquired immunodeficiency syndrome (AIDS) continue to represent a global health burden. There is currently no effective vaccine, nor any cure, for HIV infections; existing antir…
View article: 137 FcγRIIa-specific DARPins as a novel tool toward specific gene delivery into HIV reservoir cells
137 FcγRIIa-specific DARPins as a novel tool toward specific gene delivery into HIV reservoir cells Open
Background FcγRIIa (CD32a) has recently been identified as a cellular marker for the latent HIV-1 reservoir.1 Its relevance as a marker has been challenged, due to technical problems complicated by T-B cell conjugates, and trogo…
View article: Silent codon positions in the A-rich HIV RNA genome that do not easily become A: Restrictions imposed by the RNA sequence and structure
Silent codon positions in the A-rich HIV RNA genome that do not easily become A: Restrictions imposed by the RNA sequence and structure Open
There is a strong evolutionary tendency of the human immunodeficiency virus (HIV) to accumulate A nucleotides in its RNA genome, resulting in a mere 40 per cent A count. This A bias is especially dominant for the so-called silent codon pos…
View article: HIV-phyloTSI: Subtype-independent estimation of time since HIV-1 infection for cross-sectional measures of population incidence using deep sequence data
HIV-phyloTSI: Subtype-independent estimation of time since HIV-1 infection for cross-sectional measures of population incidence using deep sequence data Open
Estimating the time since HIV infection (TSI) at population level is essential for tracking changes in the global HIV epidemic. Most methods for determining duration of infection classify samples into recent and non-recent and are unable t…
View article: Novel role of UHRF1 in the epigenetic repression of the latent HIV-1
Novel role of UHRF1 in the epigenetic repression of the latent HIV-1 Open
View article: Novel role of UHRF1 in the epigenetic repression of the latent HIV-1
Novel role of UHRF1 in the epigenetic repression of the latent HIV-1 Open
Funding was provided by the Belgian National Fund for Scientific Research (F.R.S.-FNRS, Belgium), the « Fondation Roi Baudouin », the NEAT (European AIDS Treatment Network) program, the Internationale Brachet Stiftung, ViiV Healthcare, the…