Benjamin E. Draper
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View article: Toward an all-in-one recombinant adeno-associated virus vector for functionally ablating the prion gene using CRISPR-Cas technology
Toward an all-in-one recombinant adeno-associated virus vector for functionally ablating the prion gene using CRISPR-Cas technology Open
Any strategy that can selectively and persistently lower the brain levels of the cellular prion protein (PrP C ) is expected to extend survival in prion diseases. Recent advances in the virus-mediated delivery of gene therapies prompted us…
View article: End-to-end characterization of AAV manufacturing process using charge detection mass spectrometry
End-to-end characterization of AAV manufacturing process using charge detection mass spectrometry Open
[This corrects the article DOI: 10.1016/j.omtm.2025.101607.].
View article: End-to-end characterization of AAV manufacturing process using charge detection mass spectrometry
End-to-end characterization of AAV manufacturing process using charge detection mass spectrometry Open
Charge detection mass spectrometry (CD-MS) has previously been shown to be effective for analyzing predominantly clean bulk drug substances and drug products of recombinant adeno-associated virus (rAAV). CD-MS holds potential for assessing…
View article: Insights into Adeno-Associated Virus Capsid Charge Heterogeneity
Insights into Adeno-Associated Virus Capsid Charge Heterogeneity Open
A comprehensive workflow is described to examine three contributing factors to the charge heterogeneity of Adeno-associated viruses (AAVs) from a single sample. Intact AAV9 capsids were fractionated using imaged capillary isoelectric focus…
View article: Coupling of Size Exclusion Chromatography to High Throughput Charge Detection Mass Spectrometry for the Analysis of Large Proteins and Virus-like Particles
Coupling of Size Exclusion Chromatography to High Throughput Charge Detection Mass Spectrometry for the Analysis of Large Proteins and Virus-like Particles Open
Charge detection mass spectrometry (CD-MS) is an emerging single-particle technique where both the m/z and charge are measured individually to determine each ion's mass. It is particularly well-suited for analyzing high mass and heterogene…
View article: Complementary Nanoparticle Characterization by Resistive-Pulse Sensing, Electron Microscopy, and Charge Detection Mass Spectrometry
Complementary Nanoparticle Characterization by Resistive-Pulse Sensing, Electron Microscopy, and Charge Detection Mass Spectrometry Open
Nanotechnology has provided novel modalities for the delivery of therapeutic and diagnostic agents. In particular, nanoparticles (NPs) can be engineered at a low cost for drug loading and delivery. For example, silica NPs have proven usefu…
View article: Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages
Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages Open
Protein capsids are a widespread form of compartmentalization in nature. Icosahedral symmetry is ubiquitous in capsids derived from spherical viruses, as this geometry maximizes the internal volume that can be enclosed within. Despite the …
View article: Multiple Ion Charge Extraction (MICE) for High-Throughput Charge Detection Mass Spectrometry
Multiple Ion Charge Extraction (MICE) for High-Throughput Charge Detection Mass Spectrometry Open
Charge detection mass spectrometry (CD-MS) is a single-particle technique, where the masses of individual ions are determined from simultaneous measurements of their mass-to-charge ratio (m/z) and charge. The ions are trapped in an electro…
View article: Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages
Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages Open
Protein capsids are a widespread form of compartmentalisation in nature. Icosahedral symmetry is ubiquitous in capsids derived from spherical viruses, as this geometry maximises the internal volume that can be enclosed within. Despite the …
View article: Recombinant AAV genome size effect on viral vector production, purification, and thermostability
Recombinant AAV genome size effect on viral vector production, purification, and thermostability Open
Adeno-associated virus (AAV) has shown great promise as a viral vector for gene therapy in clinical applications. The present work studied the effect of genome size on AAV production, purification, and thermostability by producing AAV2-GFP…
View article: Quantitative analysis of preferential utilization of AAV ITR as the packaging terminal signal
Quantitative analysis of preferential utilization of AAV ITR as the packaging terminal signal Open
Genetic engineering advances have led to recombinant adeno-associated virus (rAAV) becoming an invaluable tool for the development of effective gene therapies. The production of rAAV is susceptible to off-target heterogeneous packaging, th…
View article: Partial genome content within rAAVs impacts performance in a cell assay-dependent manner
Partial genome content within rAAVs impacts performance in a cell assay-dependent manner Open
Recombinant adeno-associated viruses (rAAVs) deliver DNA to numerous cell types. However, packaging of partial genomes into the rAAV capsid is of concern. Although empty rAAV capsids are studied, there is little information regarding the i…
View article: Antibody Binding to Recombinant Adeno Associated Virus Monitored by Charge Detection Mass Spectrometry
Antibody Binding to Recombinant Adeno Associated Virus Monitored by Charge Detection Mass Spectrometry Open
Recombinant adeno-associated virus (rAAV) is a leading gene therapy vector. However, neutralizing antibodies reduce its efficacy. Traditional methods used to investigate antibody binding provide limited information. Here, charge detection …
View article: Analysis of thermally driven structural changes, genome release, disassembly, and aggregation of recombinant AAV by CDMS
Analysis of thermally driven structural changes, genome release, disassembly, and aggregation of recombinant AAV by CDMS Open
Charge detection mass spectrometry (CDMS) was used to analyze recombinant adeno-associated virus serotype 8 (rAAV8) vectors after incubation at elevated temperatures. rAAV8 vectors with a range of genomes of interest (GOIs) from 2.22 to 4.…
View article: Characterization of Recombinant Chimpanzee Adenovirus C68 Low and High-Density Particles: Impact on Determination of Viral Particle Titer
Characterization of Recombinant Chimpanzee Adenovirus C68 Low and High-Density Particles: Impact on Determination of Viral Particle Titer Open
We observed differential infectivity and product yield between two recombinant chimpanzee adenovirus C68 constructs whose primary difference was genome length. To determine a possible reason for this outcome, we characterized the proportio…
View article: Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry
Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry Open
The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS). CDMS allows simultaneous …