Benjamin E. Turk
YOU?
Author Swipe
View article: Abstract 1866 Proteome-wide screening for short linear docking motifs interacting with Saccharomyces cerevisiae MAP kinases
Abstract 1866 Proteome-wide screening for short linear docking motifs interacting with Saccharomyces cerevisiae MAP kinases Open
View article: Cancer hotspot mutations rewire ERK2 specificity by selective exclusion of docking interactions
Cancer hotspot mutations rewire ERK2 specificity by selective exclusion of docking interactions Open
The protein kinase ERK2 is recurrently mutated in human squamous cell carcinomas and other tumors. ERK2 mutations cluster in an essential docking recruitment site that interacts with short linear motifs found within intrinsically disordere…
View article: Figure S5 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Figure S5 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
Figure S5: Evaluation of SETDB1 knockdown in human melanoma cells, related to Figure 4:
View article: Figure S6 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Figure S6 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
Figure S6: Characterization of possible SKO tumor antigens, related to Figure 4.
View article: Table S4 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Table S4 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
Differential expression of SKO vs YRG cells ± IFNAR blockade by RNAseq.
View article: Figure S4 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Figure S4 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
Figure S4: Evaluation of differences in SKO tumor gene expression and microenvironment profiles, related to Figure 3:
View article: Table S2 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Table S2 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
Ranked CRISPR screen dropout hits, related to Figure 1. Analysis was performed using MaGeCK.
View article: Data from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Data from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
Despite recent advances in the treatment of melanoma, many patients with metastatic disease still succumb to their disease. To identify tumor-intrinsic modulators of immunity to melanoma, we performed a whole-genome CRISPR screen in melano…
View article: Figure S1 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Figure S1 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
Figure S1: Experimental validation of SETDB1 knockout and flow gating schemes, related to Figure 4
View article: Table S3 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Table S3 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
Differential expression of SKO vs YRG cells, by RNAseq.
View article: Figure S2 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Figure S2 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
Figure S2: CRISPR screen guide distributions, related to Figure 1
View article: Figure S3 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Figure S3 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
Figure S3: Validation and characterization of Setdb1-/- cell lines, tumors, and microenvironments, related to Figure 2.
View article: Table S5 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Table S5 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
Summary of exonic mutations in YRG cells compared with YMG and WT C57BL6/J cells.
View article: Table S1 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma
Table S1 from <i>Setdb1</i> Loss Induces Type I Interferons and Immune Clearance of Melanoma Open
List of primers used to perform RT-qPCR on YUDOSO cells related to Figure S6.
View article: Aurora B controls anaphase onset and error-free chromosome segregation in trypanosomes
Aurora B controls anaphase onset and error-free chromosome segregation in trypanosomes Open
Kinetochores form the interface between chromosomes and spindle microtubules and are thus under tight control by a complex regulatory circuitry. The Aurora B kinase plays a central role within this circuitry by destabilizing improper kinet…
View article: Protein phosphatase 6 activates NF-κB to confer sensitivity to MAPK pathway inhibitors in <i>KRAS</i> - and <i>BRAF</i> -mutant cancer cells
Protein phosphatase 6 activates NF-κB to confer sensitivity to MAPK pathway inhibitors in <i>KRAS</i> - and <i>BRAF</i> -mutant cancer cells Open
The Ras–mitogen-activated protein kinase (MAPK) pathway is a major target for cancer treatment. To better understand the genetic pathways that modulate cancer cell sensitivity to MAPK pathway inhibitors, we performed a CRISPR knockout scre…
View article: The intrinsic substrate specificity of the human tyrosine kinome
The intrinsic substrate specificity of the human tyrosine kinome Open
Phosphorylation of proteins on tyrosine (Tyr) residues evolved in metazoan organisms as a mechanism of coordinating tissue growth 1 . Multicellular eukaryotes typically have more than 50 distinct protein Tyr kinases that catalyse the phosp…
View article: Distinct functional constraints driving conservation of the cofilin N-terminal regulatory tail
Distinct functional constraints driving conservation of the cofilin N-terminal regulatory tail Open
Cofilin family proteins have essential roles in remodeling the cytoskeleton through filamentous actin depolymerization and severing. The short, unstructured N-terminal region of cofilin is critical for actin binding and harbors the major s…
View article: An unconventional regulatory circuitry involving Aurora B controls anaphase onset and error-free chromosome segregation in trypanosomes
An unconventional regulatory circuitry involving Aurora B controls anaphase onset and error-free chromosome segregation in trypanosomes Open
Accurate chromosome segregation during mitosis requires that all chromosomes establish stable bi-oriented attachments with the spindle apparatus. Kinetochores form the interface between chromosomes and spindle microtubules and as such are …
View article: The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells
The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells Open
CD8 + T cells play an important role in anti-tumor immunity. Better understanding of their regulation could advance cancer immunotherapies. Here we identify, via stepwise CRISPR-based screening, that CUL5 is a negative regulator of the cor…
View article: Autoregulation of the LIM kinases by their PDZ domain
Autoregulation of the LIM kinases by their PDZ domain Open
View article: Linear motif specificity in signaling through p38α and ERK2 mitogen–activated protein kinases
Linear motif specificity in signaling through p38α and ERK2 mitogen–activated protein kinases Open
Mitogen-activated protein kinase (MAPK) cascades are essential for eukaryotic cells to integrate and respond to diverse stimuli. Maintaining specificity in signaling through MAPK networks is key to coupling distinct inputs to appropriate c…
View article: Distinct functional constraints driving conservation of the cofilin N-terminal regulatory tail
Distinct functional constraints driving conservation of the cofilin N-terminal regulatory tail Open
Summary Cofilin family proteins have essential roles in remodeling the cytoskeleton through filamentous actin depolymerization and severing. The short unstructured N-terminal region of cofilin is critical for actin binding and harbors the …
View article: An optogenetic-phosphoproteomic study reveals dynamic Akt1 signaling profiles in endothelial cells
An optogenetic-phosphoproteomic study reveals dynamic Akt1 signaling profiles in endothelial cells Open
The serine/threonine kinase AKT is a central node in cell signaling. While aberrant AKT activation underlies the development of a variety of human diseases, how different patterns of AKT-dependent phosphorylation dictate downstream signali…
View article: <i>Setdb1</i>-loss induces type-I interferons and immune clearance of melanoma
<i>Setdb1</i>-loss induces type-I interferons and immune clearance of melanoma Open
Summary Despite recent advances in the treatment of melanoma, many patients with metastatic disease still succumb to their disease. To identify tumor-intrinsic modulators of immunity to melanoma, we performed a whole-genome CRISPR screen i…
View article: Supplementary Tables from TNIK Is a Therapeutic Target in Lung Squamous Cell Carcinoma and Regulates FAK Activation through Merlin
Supplementary Tables from TNIK Is a Therapeutic Target in Lung Squamous Cell Carcinoma and Regulates FAK Activation through Merlin Open
Supplementary Tables
View article: Supplementary Methods from TNIK Is a Therapeutic Target in Lung Squamous Cell Carcinoma and Regulates FAK Activation through Merlin
Supplementary Methods from TNIK Is a Therapeutic Target in Lung Squamous Cell Carcinoma and Regulates FAK Activation through Merlin Open
Supplementary Methods
View article: Data from TNIK Is a Therapeutic Target in Lung Squamous Cell Carcinoma and Regulates FAK Activation through Merlin
Data from TNIK Is a Therapeutic Target in Lung Squamous Cell Carcinoma and Regulates FAK Activation through Merlin Open
Lung squamous cell carcinoma (LSCC) is the second most prevalent type of lung cancer. Despite extensive genomic characterization, no targeted therapies are approved for the treatment of LSCC. Distal amplification of the 3q chromosome is th…
View article: Supplementary Figure S8.1 from TNIK Is a Therapeutic Target in Lung Squamous Cell Carcinoma and Regulates FAK Activation through Merlin
Supplementary Figure S8.1 from TNIK Is a Therapeutic Target in Lung Squamous Cell Carcinoma and Regulates FAK Activation through Merlin Open
Uncropped immunoblots reported in this study.
View article: Supplementary Movie 1 from TNIK Is a Therapeutic Target in Lung Squamous Cell Carcinoma and Regulates FAK Activation through Merlin
Supplementary Movie 1 from TNIK Is a Therapeutic Target in Lung Squamous Cell Carcinoma and Regulates FAK Activation through Merlin Open
Supplementary Movie depicting the proposed mechanism through which amplified TNIK contributes to LSCC cell viability