Benjamin L. Oakes
YOU?
Author Swipe
View article: Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules Open
Proteins evolve through the modular rearrangement of elements known as domains. Extant, multidomain proteins are hypothesized to be the result of domain accretion, but there has been limited experimental validation of this idea. Here, we i…
View article: Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules Open
Proteins evolve through the modular rearrangement of elements known as domains. It is hypothesized that extant, multidomain proteins are the result of domain accretion, but there has been limited experimental validation of this idea. Here,…
View article: A yeast optogenetic toolkit (yOTK) for gene expression control in <i>Saccharomyces cerevisiae</i>
A yeast optogenetic toolkit (yOTK) for gene expression control in <i>Saccharomyces cerevisiae</i> Open
Optogenetic tools for controlling gene expression are ideal for tuning synthetic biological networks due to the exquisite spatiotemporal control available with light. Here we develop an optogenetic system for gene expression control integr…
View article: A yeast optogenetic toolkit (yOTK) for gene expression control in <i>Saccharomyces cerevisiae</i>
A yeast optogenetic toolkit (yOTK) for gene expression control in <i>Saccharomyces cerevisiae</i> Open
Optogenetic tools for controlling gene expression are ideal for tuning synthetic biological networks due to the exquisite spatiotemporal control available with light. Here we develop an optogenetic system for gene expression control and in…
View article: Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs Open
The CRISPR-Cas9 system provides the ability to edit, repress, activate, or mark any gene (or DNA element) by pairing of a programmable single guide RNA (sgRNA) with a complementary sequence on the DNA target. Here we present a new method f…
View article: Engineering CRISPR-Cas9 Systems to Expand Functionality
Engineering CRISPR-Cas9 Systems to Expand Functionality Open
CRISPR-Cas9 is a RNA-protein complex from adaptive bacterial immune systems that has been repurposed to enable convenient and specific alteration of any genome via the introduction of a double-strand DNA break. By its complementarity to a …
View article: A CubeSat-Based Optical Communication Network for Low Earth Orbit
A CubeSat-Based Optical Communication Network for Low Earth Orbit Open
A common challenge in developing small-satellite-based Earth-observation (EO) missions is getting the data to the ground. Typically, most satellites download data via space-to-ground radio-frequency (RF) links, communicating directly with …
View article: Multi-reporter selection for the design of active and more specific zinc-finger nucleases for genome editing
Multi-reporter selection for the design of active and more specific zinc-finger nucleases for genome editing Open
Engineered nucleases have transformed biological research and offer great therapeutic potential by enabling the straightforward modification of desired genomic sequences. While many nuclease platforms have proven functional, all can produc…