Benjamin P. Bratton
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View article: Sensing the shape of a surface by tightly surface-bound filaments
Sensing the shape of a surface by tightly surface-bound filaments Open
Understanding the mechanisms that dictate the localization of cytoskeletal filaments is crucial for elucidating cell shape regulation in prokaryotes. The actin homolog MreB plays a pivotal role in maintaining the shape of many rod-shaped b…
View article: An Anaerobic Pathogen Rewires Host Metabolism to Fuel Oxidative Growth in the Inflamed Gut
An Anaerobic Pathogen Rewires Host Metabolism to Fuel Oxidative Growth in the Inflamed Gut Open
To colonize their host and cause disease, enteric pathogens must deploy their virulence factors to establish distinct nutrient niches. How obligate anaerobic pathogens construct nutrient niches in the densely populated large intestine rema…
View article: Macromolecular interactions and geometrical confinement determine the 3D diffusion of ribosome-sized particles in live <i>Escherichia coli</i> cells
Macromolecular interactions and geometrical confinement determine the 3D diffusion of ribosome-sized particles in live <i>Escherichia coli</i> cells Open
The crowded bacterial cytoplasm is composed of biomolecules that span several orders of magnitude in size and electrical charge. This complexity has been proposed as the source of the rich spatial organization and apparent anomalous diffus…
View article: Sensing the shape of a surface by intracellular filaments
Sensing the shape of a surface by intracellular filaments Open
Understanding the mechanisms that dictate the localization of cytoskeletal filaments is crucial for elucidating cell shape regulation in prokaryotes. The actin homolog MreB plays a pivotal role in maintaining the shape of many rod-shaped b…
View article: Evolution of pH-sensitive transcription termination in <i>Escherichia coli</i> during adaptation to repeated long-term starvation
Evolution of pH-sensitive transcription termination in <i>Escherichia coli</i> during adaptation to repeated long-term starvation Open
Fluctuating environments that consist of regular cycles of co-occurring stress are a common challenge faced by cellular populations. For a population to thrive in constantly changing conditions, an ability to coordinate a rapid cellular re…
View article: Bacteria can anticipate the seasons: Photoperiodism in cyanobacteria
Bacteria can anticipate the seasons: Photoperiodism in cyanobacteria Open
Photoperiodic time measurement is the ability of plants and animals to measure differences in day versus night length (photoperiod) and use that information to anticipate critical seasonal transformations, such as annual temperature cycles…
View article: Global protein turnover quantification in Escherichia coli reveals cytoplasmic recycling under nitrogen limitation
Global protein turnover quantification in Escherichia coli reveals cytoplasmic recycling under nitrogen limitation Open
Protein turnover is critical for proteostasis, but turnover quantification is challenging, and even in well-studied E. coli , proteome-wide measurements remain scarce. Here, we quantify the turnover rates of ~3200 E. coli proteins under 13…
View article: Macromolecular interactions and geometrical confinement determine the 3D diffusion of ribosome-sized particles in live<i>Escherichia coli</i>cells
Macromolecular interactions and geometrical confinement determine the 3D diffusion of ribosome-sized particles in live<i>Escherichia coli</i>cells Open
The crowded bacterial cytoplasm is comprised of biomolecules that span several orders of magnitude in size and electrical charge. This complexity has been proposed as the source of the rich spatial organization and apparent anomalous diffu…
View article: Evolution of pH-sensitive transcription termination during adaptation to repeated long-term starvation
Evolution of pH-sensitive transcription termination during adaptation to repeated long-term starvation Open
Fluctuating environments that consist of regular cycles of co-occurring stress are a common challenge faced by cellular populations. For a population to thrive in constantly changing conditions, an ability to coordinate a rapid cellular re…
View article: Integrin-like adhesin CglD confers traction and stabilizes bacterial focal adhesions involved in myxobacterial gliding motility
Integrin-like adhesin CglD confers traction and stabilizes bacterial focal adhesions involved in myxobacterial gliding motility Open
A bstract Integrins are crucial for eukaryotic cell attachment and motility within the extracellular matrix (ECM) via focal-adhesion formation, with their evolutionary emergence important for the development of multicellularity. Intriguing…
View article: Bacterial DNA on the skin surface overrepresents the viable skin microbiome
Bacterial DNA on the skin surface overrepresents the viable skin microbiome Open
The skin microbiome provides vital contributions to human health. However, the spatial organization and viability of its bacterial components remain unclear. Here, we apply culturing, imaging, and molecular approaches to human and mouse sk…
View article: Reviewer #1 (Public Review): Bacterial DNA on the skin surface overrepresents the viable skin microbiome
Reviewer #1 (Public Review): Bacterial DNA on the skin surface overrepresents the viable skin microbiome Open
The skin microbiome provides vital contributions to human health. However, the spatial organization and viability of its bacterial components remain unclear. Here we apply culturing, imaging, and molecular approaches to human and mouse ski…
View article: Reviewer #2 (Public Review): Bacterial DNA on the skin surface overrepresents the viable skin microbiome
Reviewer #2 (Public Review): Bacterial DNA on the skin surface overrepresents the viable skin microbiome Open
The skin microbiome provides vital contributions to human health. However, the spatial organization and viability of its bacterial components remain unclear. Here we apply culturing, imaging, and molecular approaches to human and mouse ski…
View article: Bacterial DNA on the skin surface overrepresents the viable skin microbiome
Bacterial DNA on the skin surface overrepresents the viable skin microbiome Open
The skin microbiome provides vital contributions to human health. However, the spatial organization and viability of its bacterial components remain unclear. Here we apply culturing, imaging, and molecular approaches to human and mouse ski…
View article: Bacterial DNA on the skin surface overrepresents the viable skin microbiome
Bacterial DNA on the skin surface overrepresents the viable skin microbiome Open
The skin microbiome provides vital contributions to human health. However, the spatial organization and viability of its bacterial components remain unclear. Here, we apply culturing, imaging, and molecular approaches to human and mouse sk…
View article: Unmasking of the von Willebrand A-domain surface adhesin CglB at bacterial focal adhesions mediates myxobacterial gliding motility
Unmasking of the von Willebrand A-domain surface adhesin CglB at bacterial focal adhesions mediates myxobacterial gliding motility Open
The predatory deltaproteobacterium Myxococcus xanthus uses a helically-trafficked motor at bacterial focal-adhesion (bFA) sites to power gliding motility. Using total internal reflection fluorescence and force microscopies, we identify the…
View article: CauloKO: an ordered transposon mutant library in<i>Caulobacter crescentus</i>
CauloKO: an ordered transposon mutant library in<i>Caulobacter crescentus</i> Open
Genetic screens are powerful approaches to unveiling new biological insight and ordered redundant transposon libraries have emerged as a primary tool for performing screens of known genetic saturation. Newer sequencing methods based on com…
View article: Distinct regions of H. pylori’s bactofilin CcmA regulate protein–protein interactions to control helical cell shape
Distinct regions of H. pylori’s bactofilin CcmA regulate protein–protein interactions to control helical cell shape Open
The helical shape of Helicobacter pylori cells promotes robust stomach colonization; however, how the helical shape of H. pylori cells is determined is unresolved. Previous work identified helical-cell-shape-promoting protein complexes con…
View article: Author response: Distinct regions of H. pylori’s bactofilin CcmA regulate protein–protein interactions to control helical cell shape
Author response: Distinct regions of H. pylori’s bactofilin CcmA regulate protein–protein interactions to control helical cell shape Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract The helical shape of H…
View article: Global Protein-Turnover Quantification in<i>Escherichia coli</i>Reveals Cytoplasmic Recycling under Nitrogen Limitation
Global Protein-Turnover Quantification in<i>Escherichia coli</i>Reveals Cytoplasmic Recycling under Nitrogen Limitation Open
Summary Protein turnover is critical for proteostasis, but turnover quantification is challenging, and even in well-studied E. coli , proteome-wide measurements remain scarce. Here, we quantify the degradation rates of ∼3.2k E. coli protei…
View article: Pseudomonas aeruginosa clinical blood isolates display significant phenotypic variability
Pseudomonas aeruginosa clinical blood isolates display significant phenotypic variability Open
Pseudomonas aeruginosa is a significant threat in healthcare settings where it deploys a wide host of virulence factors to cause disease. Many virulence-related phenotypes such as pyocyanin production, biofilm formation, and twitching moti…
View article: Distinct regions of <i>H. pylori’s</i> bactofilin CcmA regulate protein-protein interactions to control helical cell shape
Distinct regions of <i>H. pylori’s</i> bactofilin CcmA regulate protein-protein interactions to control helical cell shape Open
The helical shape of H. pylori cells promotes robust stomach colonization, however, how the helical shape of H. pylori cells is determined is unresolved. Previous work identified helical-cell-shape-promoting protein complexes containing a …
View article: Decision letter: Distinguishing different modes of growth using single-cell data
Decision letter: Distinguishing different modes of growth using single-cell data Open
Article Figures and data Abstract Editor's evaluation eLife digest Introduction Results Discussion Materials and methods Appendix 1 Appendix 2 Data availability References Decision letter Author response Article and author information Metr…
View article: Bacterial DNA on the skin surface overrepresents the viable skin microbiome
Bacterial DNA on the skin surface overrepresents the viable skin microbiome Open
The skin microbiome provides vital contributions to human health. However, the spatial organization and viability of its bacterial components remain unclear. Here we apply culturing, imaging, and molecular approaches to human and mouse ski…
View article: CglB adhesins secreted at bacterial focal adhesions mediate gliding motility
CglB adhesins secreted at bacterial focal adhesions mediate gliding motility Open
A bstract The predatory deltaproteobacterium Myxococcus xanthus uses a helically-trafficked motor at bacterial focal adhesion (bFA) sites to power gliding motility. Using TIRF and force microscopy, we herein identify the integrin αI-domain…
View article: <i>Pseudomonas aeruginosa</i>detachment from surfaces via a self-made small molecule
<i>Pseudomonas aeruginosa</i>detachment from surfaces via a self-made small molecule Open
Pseudomonas aeruginosa is a significant threat in both healthcare and industrial biofouling. Surface attachment of P. aeruginosa is particularly problematic as surface association induces virulence and biofilm formation, which hamper later…