Benjamin S. Schuster
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View article: POMPOMS: Crosslinked Biomolecular Condensates as a Versatile Platform for Multifunctional Protein Microparticles
POMPOMS: Crosslinked Biomolecular Condensates as a Versatile Platform for Multifunctional Protein Microparticles Open
Protein-based microparticles are promising materials for applications such as biocatalysis and biomolecular capture, yet their fabrication by existing techniques remains challenging due to protein denaturation or lack of spatial control. H…
View article: Amphiphilic Protein Surfactants Reduce the Interfacial Tension of Biomolecular Condensates
Amphiphilic Protein Surfactants Reduce the Interfacial Tension of Biomolecular Condensates Open
Biomolecular condensates are protein-dense regions in cells that often arise from liquid-liquid phase separation. Interfacial tension is a key determinant of biomolecular condensate behavior, influencing condensate size and interactions wi…
View article: Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states Open
View article: Amphiphilic protein surfactants reduce the interfacial tension of biomolecular condensates
Amphiphilic protein surfactants reduce the interfacial tension of biomolecular condensates Open
Biomolecular condensates are protein-dense regions in cells that often arise from liquid-liquid phase separation. Interfacial tension is a key determinant of biomolecular condensate behavior, influencing condensate size and interactions wi…
View article: POMPOMS: Crosslinked biomolecular condensates as a versatile platform for multifunctional protein microparticles
POMPOMS: Crosslinked biomolecular condensates as a versatile platform for multifunctional protein microparticles Open
Protein-based microparticles are promising materials for applications such as biocatalysis and biomolecular capture, yet their fabrication by existing techniques remains challenging due to protein denaturation or lack of spatial control. H…
View article: Controlled and orthogonal partitioning of large particles into biomolecular condensates
Controlled and orthogonal partitioning of large particles into biomolecular condensates Open
Partitioning of client molecules into biomolecular condensates is critical for regulating the composition and function of condensates. Previous studies suggest that client size limits partitioning. Here, we ask whether large clients, such …
View article: Phosphorylation Toggles the SARS-CoV-2 Nucleocapsid Protein Between Two Membrane-Associated Condensate States
Phosphorylation Toggles the SARS-CoV-2 Nucleocapsid Protein Between Two Membrane-Associated Condensate States Open
Summary The Nucleocapsid protein (N) of SARS-CoV-2 plays a critical role in the viral lifecycle by regulating RNA replication and packaging the viral genome. N and RNA phase separate to form condensates that may be important for these func…
View article: Controlled and orthogonal partitioning of large particles into biomolecular condensates
Controlled and orthogonal partitioning of large particles into biomolecular condensates Open
Biomolecular condensates arising from liquid-liquid phase separation contribute to diverse cellular processes, such as gene expression. Partitioning of client molecules into condensates is critical to regulating the composition and functio…
View article: Expanding the molecular language of protein liquid–liquid phase separation
Expanding the molecular language of protein liquid–liquid phase separation Open
View article: Revealing nanoscale structure and interfaces of protein and polymer condensates via cryo-electron microscopy
Revealing nanoscale structure and interfaces of protein and polymer condensates via cryo-electron microscopy Open
Liquid-liquid phase separation (LLPS) is a ubiquitous demixing phenomenon observed in various molecular solutions, including in polymer and protein solutions. Demixing of solutions results in condensed, phase separated droplets which exhib…
View article: Revealing nanoscale structure and interfaces of protein and polymer condensates <i>via</i> cryo-electron microscopy
Revealing nanoscale structure and interfaces of protein and polymer condensates <i>via</i> cryo-electron microscopy Open
This study uses cryo-electron microscopy to reveal the distinct nanoscale structures within protein condensates, highlighting the potential correlation between their internal organization and material properties.
View article: Expanding the molecular language of protein liquid-liquid phase separation
Expanding the molecular language of protein liquid-liquid phase separation Open
Understanding the relationship between an amino acid sequence and its phase separation has important implications for analyzing cellular function, treating disease, and designing novel biomaterials. Several sequence features have been iden…
View article: Incorporation and assembly of a light-emitting enzymatic reaction into model protein condensates
Incorporation and assembly of a light-emitting enzymatic reaction into model protein condensates Open
View article: Amphiphilic proteins coassemble into multiphasic condensates and act as biomolecular surfactants
Amphiphilic proteins coassemble into multiphasic condensates and act as biomolecular surfactants Open
Significance Membraneless organelles are assemblies of highly concentrated biomolecules that condense through liquid–liquid phase separation. One major question in the field is how proteins assemble into multilayered condensates. Understan…
View article: Incorporation and Assembly of a Light-Emitting Enzymatic Reaction into Model Protein Condensates
Incorporation and Assembly of a Light-Emitting Enzymatic Reaction into Model Protein Condensates Open
Eukaryotic cells partition enzymes and other cellular components into distinct subcellular compartments to generate specialized biochemical niches. A subclass of these compartments form in the absence of lipid membranes, via liquid-liquid …
View article: Designer membraneless organelles sequester native factors for control of cell behavior
Designer membraneless organelles sequester native factors for control of cell behavior Open
View article: Surface tension and viscosity of protein condensates quantified by micropipette aspiration
Surface tension and viscosity of protein condensates quantified by micropipette aspiration Open
The material properties of biomolecular condensates have been suggested to play important biological and pathological roles. Despite the rapid increase in the number of biomolecules identified that undergo liquid-liquid phase separation, q…
View article: More than just oil droplets in water: surface tension and viscosity of protein condensates quantified by micropipette aspiration
More than just oil droplets in water: surface tension and viscosity of protein condensates quantified by micropipette aspiration Open
The material properties of biomolecular condensates play pivotal roles in many biological and pathological processes. Despite the rapid increase in the number of biomolecules identified that undergo liquid-liquid phase separation (LLPS), q…
View article: Amphiphilic proteins coassemble into multiphasic condensates and act as biomolecular surfactants
Amphiphilic proteins coassemble into multiphasic condensates and act as biomolecular surfactants Open
Cells contain membraneless compartments that assemble due to liquid-liquid phase separation, including biomolecular condensates with complex morphologies. For instance, certain condensates are surrounded by a film of distinct composition, …
View article: Biomolecular Condensates: Sequence Determinants of Phase Separation, Microstructural Organization, Enzymatic Activity, and Material Properties
Biomolecular Condensates: Sequence Determinants of Phase Separation, Microstructural Organization, Enzymatic Activity, and Material Properties Open
This perspective article highlights recent progress and emerging challenges in understanding the formation and function of membraneless organelles (MLOs). A long-term goal in the MLO field is to identify the sequence-encoded rules that dic…
View article: Amphiphilic Proteins Coat Membraneless Organelles and Act as Biological Surfactants
Amphiphilic Proteins Coat Membraneless Organelles and Act as Biological Surfactants Open
View article: Identifying sequence perturbations to an intrinsically disordered protein that determine its phase-separation behavior
Identifying sequence perturbations to an intrinsically disordered protein that determine its phase-separation behavior Open
Significance Membraneless organelles are assemblies of highly concentrated biomolecules that form through liquid–liquid phase separation. These assemblies are often enriched in intrinsically disordered proteins (IDPs), which play an import…
View article: SPLIT: Stable Protein Coacervation Using a Light Induced Transition
SPLIT: Stable Protein Coacervation Using a Light Induced Transition Open
Protein coacervates serve as hubs to concentrate and sequester proteins and nucleotides and thus function as membraneless organelles to manipulate cell physiology. We have engineered a coacervating protein to create tunable, synthetic memb…
View article: Identifying Sequence Perturbations to an Intrinsically Disordered Protein that Determine Its Phase Separation Behavior
Identifying Sequence Perturbations to an Intrinsically Disordered Protein that Determine Its Phase Separation Behavior Open
Phase separation of intrinsically disordered proteins (IDPs) commonly underlies the formation of membraneless organelles, which compartmentalize molecules intracellularly in the absence of a lipid membrane. Identifying the protein sequence…
View article: Sequence Determinants of Protein Phase Separation of the Intrinsically Disordered RGG Domain from LAF-1
Sequence Determinants of Protein Phase Separation of the Intrinsically Disordered RGG Domain from LAF-1 Open
View article: Outcomes after placement of a SX‐ELLA oesophageal stent for refractory variceal bleeding—A national multicentre study
Outcomes after placement of a SX‐ELLA oesophageal stent for refractory variceal bleeding—A national multicentre study Open
Background Current guidelines favour the use of bleeding stents over balloon tamponade (BT) for refractory variceal bleeding (VB) from oesophageal varices. However, data on the efficacy and safety of self‐expandable metal SX‐ELLA Danis ste…
View article: Directly light-regulated binding of RGS-LOV photoreceptors to anionic membrane phospholipids
Directly light-regulated binding of RGS-LOV photoreceptors to anionic membrane phospholipids Open
Significance Light–oxygen–voltage (LOV) domain photoreceptors are found ubiquitously in nature and possess highly diverse signaling roles and mechanisms. Here, we show that a class of fungal LOV proteins dynamically associates with anionic…
View article: Controllable protein phase separation and modular recruitment to form responsive membraneless organelles
Controllable protein phase separation and modular recruitment to form responsive membraneless organelles Open
View article: Optochemical Control of Protein Localization and Activity within Cell-like Compartments
Optochemical Control of Protein Localization and Activity within Cell-like Compartments Open
We report inducible dimerization strategies for controlling protein positioning, enzymatic activity, and organelle assembly inside synthetic cell-like compartments upon photostimulation. Using a photocaged TMP-Haloligand compound, we demon…
View article: Controllable Protein Phase Separation and Modular Recruitment to Investigate Biochemical Compartmentalization in Membraneless Organelles
Controllable Protein Phase Separation and Modular Recruitment to Investigate Biochemical Compartmentalization in Membraneless Organelles Open