Benjamin Spaniol
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View article: Complexome profiling of the Chlamydomonas <i>psb28</i> mutant reveals TEF5 as an early PSII assembly factor
Complexome profiling of the Chlamydomonas <i>psb28</i> mutant reveals TEF5 as an early PSII assembly factor Open
PSII assembly requires auxiliary factors, including Psb28. Although the absence of Psb28 in cyanobacteria has little effect on PSII assembly, we show here that the Chlamydomonas (Chlamydomonas reinhardtii) psb28 null mutant is severely imp…
View article: Complexome profiling of the Chlamydomonas<i>psb28</i>mutant reveals THYLAKOID ENRICHED FRACTION 5 as an early photosystem II assembly factor
Complexome profiling of the Chlamydomonas<i>psb28</i>mutant reveals THYLAKOID ENRICHED FRACTION 5 as an early photosystem II assembly factor Open
Several auxiliary factors are required for the assembly of photosystem (PS) II, one of which is Psb28. While the absence of Psb28 in cyanobacteria has little effect on PSII assembly, we show here that the Chlamydomonas psb28 -null mutant i…
View article: Fatty acid export (FAX) proteins contribute to oil production in the green microalga Chlamydomonas reinhardtii
Fatty acid export (FAX) proteins contribute to oil production in the green microalga Chlamydomonas reinhardtii Open
In algae and land plants, transport of fatty acids (FAs) from their site of synthesis in the plastid stroma to the endoplasmic reticulum (ER) for assembly into acyl lipids is crucial for cellular lipid homeostasis, including the biosynthes…
View article: Complexome profiling on the <i>Chlamydomonas lpa2</i> mutant reveals insights into PSII biogenesis and new PSII associated proteins
Complexome profiling on the <i>Chlamydomonas lpa2</i> mutant reveals insights into PSII biogenesis and new PSII associated proteins Open
While the composition and function of the major thylakoid membrane complexes are well understood, comparatively little is known about their biogenesis. The goal of this work was to shed more light on the role of auxiliary factors in the bi…
View article: Complexome profiling on the<i>lpa2</i>mutant reveals insights into PSII biogenesis and new PSII associated proteins
Complexome profiling on the<i>lpa2</i>mutant reveals insights into PSII biogenesis and new PSII associated proteins Open
We have identified the homolog of LOW PSII ACCUMULATION 2 (LPA2) in Chlamydomonas . A Chlamydomonas lpa2 mutant grew slower in low light and was hypersensitive to high light. PSII maximum quantum efficiency was reduced by 38%. Synthesis an…
View article: The <i>Chlamydomonas deg1c</i> Mutant Accumulates Proteins Involved in High Light Acclimation
The <i>Chlamydomonas deg1c</i> Mutant Accumulates Proteins Involved in High Light Acclimation Open
Degradation of periplasmic proteins (Deg)/high temperature requirement A (HtrA) proteases are ATP-independent Ser endopeptidases that perform key aspects of protein quality control in all domains of life. Here, we characterized Chlamydomon…
View article: Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga <i>Chlamydomonas reinhardtii</i>
Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga <i>Chlamydomonas reinhardtii</i> Open
Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factor…