Benjamin Wiseman
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View article: CryoEM of ATP-driven dynamics and itraconazole binding in a fungal ABC pump
CryoEM of ATP-driven dynamics and itraconazole binding in a fungal ABC pump Open
Azole resistance in Candida species often results from overexpression of the ABC transporter Cdr1, which expels drugs via ATP-driven conformational changes. Despite its clinical relevance, the structural basis of Cdr1 function has remained…
View article: ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat
ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat Open
View article: Purification and characterization of Cdr1, the drug-efflux pump conferring azole resistance in Candida species
Purification and characterization of Cdr1, the drug-efflux pump conferring azole resistance in Candida species Open
View article: Alternating L4 loop architecture of the bacterial polysaccharide co-polymerase WzzE
Alternating L4 loop architecture of the bacterial polysaccharide co-polymerase WzzE Open
View article: Architecture of the vertebrate egg coat and structural basis of the ZP2 block to polyspermy
Architecture of the vertebrate egg coat and structural basis of the ZP2 block to polyspermy Open
SUMMARY Post-fertilization cleavage of glycoprotein ZP2, a major subunit of egg zona pellucida (ZP) filaments, is crucial for mammalian reproduction by irreversibly blocking polyspermy. ZP2 processing is thought to inactivate a sperm-bindi…
View article: 1-methylcyclopropene and Harvest Maturity Impact ‘Ma’afala’ Breadfruit Postharvest Storage
1-methylcyclopropene and Harvest Maturity Impact ‘Ma’afala’ Breadfruit Postharvest Storage Open
Breadfruit marketing is limited by its rapid ripening and deterioration after harvest; therefore, improved postharvest practices may facilitate breadfruit marketing. This study examined the effect of harvest maturity and 1-methylcycloprope…
View article: Architecture of the Vertebrate Egg Coat and Structural Basis of the ZP2 Block to Polyspermy
Architecture of the Vertebrate Egg Coat and Structural Basis of the ZP2 Block to Polyspermy Open
View article: Pineapple Field Establishment Using Slips
Pineapple Field Establishment Using Slips Open
Slips are side-shoots or fruit with large crowns that grow from buds on the pineapple ( Ananas comosus L.) peduncle. The slips are widely used for pineapple vegetative propagation when crowns are left attached to the fruit that is marketed…
View article: Substrate-bound and substrate-free outward-facing structures of a multidrug ABC exporter
Substrate-bound and substrate-free outward-facing structures of a multidrug ABC exporter Open
Multidrug ABC transporters translocate drugs across membranes by a mechanism for which the molecular features of drug release are so far unknown. Here, we resolved three ATP-Mg 2+ –bound outward-facing conformations of the Bacillus subtili…
View article: Drug-bound and -free outward-facing structures of a multidrug ABC exporter point to a swing mechanism
Drug-bound and -free outward-facing structures of a multidrug ABC exporter point to a swing mechanism Open
Multidrug ABC transporters translocate drugs across membranes by a mechanism for which the molecular features of drug release are so far unknown. Here, we resolved two ATP-Mg 2+ -bound outward-facing (OF) conformations of the Bacillus subt…
View article: Structure of a full-length bacterial polysaccharide co-polymerase
Structure of a full-length bacterial polysaccharide co-polymerase Open
View article: Conformational changes in Apolipoprotein N-acyltransferase (Lnt)
Conformational changes in Apolipoprotein N-acyltransferase (Lnt) Open
View article: Conformational changes in Apolipoprotein N-acyltransferase (Lnt)
Conformational changes in Apolipoprotein N-acyltransferase (Lnt) Open
SUMMARY In bacteria, lipoproteins are important components of the cell envelope and are responsible for many essential cellular functions. They are produced by the post-translational covalent attachment of lipids that occurs via a sequenti…
View article: A rapid expression and purification condition screening protocol for membrane protein structural biology
A rapid expression and purification condition screening protocol for membrane protein structural biology Open
Membrane proteins control a large number of vital biological processes and are often medically important—not least as drug targets. However, membrane proteins are generally more difficult to work with than their globular counterparts, and …
View article: Diffraction anisotropy falloff in the direction of the detergent belt for two centered monoclinic crystals of OmpF
Diffraction anisotropy falloff in the direction of the detergent belt for two centered monoclinic crystals of OmpF Open
This data article describes the anisotropy of diffraction observed for the centered monoclinic crystals of OmpF reported in "Two different centered monoclinic crystals of the E. coli outer-membrane protein OmpF originate from the same buil…