Benoît Gachet
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View article: High-throughput conjugation reveals strain specific recombination patterns enabling precise trait mapping in Escherichia coli
High-throughput conjugation reveals strain specific recombination patterns enabling precise trait mapping in Escherichia coli Open
Genetic exchange is a cornerstone of evolutionary biology and genomics, driving adaptation and enabling the identification of genetic determinants underlying phenotypic traits. In Escherichia coli , horizontal gene transfer via conjugation…
View article: High-Throughput Conjugation Reveals Strain Specific Recombination Patterns Enabling Precise Trait Mapping in Escherichia coli
High-Throughput Conjugation Reveals Strain Specific Recombination Patterns Enabling Precise Trait Mapping in Escherichia coli Open
Genetic exchange is a cornerstone of evolutionary biology and genomics, driving adaptation and enabling the identification of genetic determinants underlying phenotypic traits. In Escherichia coli , horizontal gene transfer via conjugation…
View article: Not All in Vein: Oral Antibiotics for Diabetic Foot Osteomyelitis: A Narrative Review
Not All in Vein: Oral Antibiotics for Diabetic Foot Osteomyelitis: A Narrative Review Open
Introduction: Osteomyelitis is a severe complication of diabetes-related foot ulcers (DFUs) often managed with antibiotic therapy and surgical resection of the infected bone. Areas of research: While intravenous (IV) antibiotics have been …
View article: Immune checkpoint inhibitors-associated cranial nerves involvement: a systematic literature review on 136 patients
Immune checkpoint inhibitors-associated cranial nerves involvement: a systematic literature review on 136 patients Open
Objective Describe the demographic data and clinical phenotype of cranial palsy induced by immune checkpoint inhibitors (CNP-ICI). Methods A systematic literature review of the literature was performed in Pubmed, Web of Science, and Embase…
View article: IncC plasmid genome rearrangements influence the vertical and horizontal transmission tradeoff in <i>Escherichia coli</i>
IncC plasmid genome rearrangements influence the vertical and horizontal transmission tradeoff in <i>Escherichia coli</i> Open
It has been shown that an evolutionary tradeoff between vertical (host growth rate) and horizontal (plasmid conjugation) transmissions contributes to global plasmid fitness. As conjugative IncC plasmids are important for the spread of mult…
View article: Outcomes in orthopedic device infections due to Streptococcus agalactiae: a retrospective cohort study
Outcomes in orthopedic device infections due to Streptococcus agalactiae: a retrospective cohort study Open
Background Group B streptococci (S treptococcus agalactiae ) (GBS) is a rare cause of prosthetic joint infection (PJI) occurring in patients with comorbidities and seems to be associated with a poor outcome. Depiction of GBS PJI is scarce …
View article: IncC plasmid genome rearrangements influence the vertical and horizontal transmission tradeoff in<i>Escherichia coli</i>
IncC plasmid genome rearrangements influence the vertical and horizontal transmission tradeoff in<i>Escherichia coli</i> Open
It has been shown that an evolutionary tradeoff between vertical (host growth rate) and horizontal (plasmid conjugation) transmissions contribute to global plasmid fitness. As conjugative IncC plasmids are important for the spread of multi…
View article: Prevalence, causes, and clinical associations of anemia in patients with systemic sclerosis: A cohort study
Prevalence, causes, and clinical associations of anemia in patients with systemic sclerosis: A cohort study Open
Background: Anemia is considered a risk factor of severity in systemic sclerosis. Yet, limited data are available on the frequency and causes of anemia in systemic sclerosis. The objectives of our study were to determine the frequency and …
View article: Deep mutational scanning reveals the molecular determinants of RNA polymerase-mediated adaptation and tradeoffs
Deep mutational scanning reveals the molecular determinants of RNA polymerase-mediated adaptation and tradeoffs Open
RNA polymerase (RNAP) is emblematic of complex biological systems that control multiple traits involving trade-offs such as growth versus maintenance. Laboratory evolution has revealed that mutations in RNAP subunits, including RpoB, are f…
View article: Dissemination of IncI plasmid encoding <i>bla</i> <sub>CTX-M-1</sub> is not hampered by its fitness cost in the pig’s gut
Dissemination of IncI plasmid encoding <i>bla</i> <sub>CTX-M-1</sub> is not hampered by its fitness cost in the pig’s gut Open
Multiresistance plasmids belonging to the IncI incompatibility group have become one of the most pervasive plasmid types in extended-spectrum beta-lactamase-producing Escherichia coli of animal origin. The extent of the burden imposed on t…
View article: The bacterial genetic determinants of Escherichia coli capacity to cause bloodstream infections in humans
The bacterial genetic determinants of Escherichia coli capacity to cause bloodstream infections in humans Open
Escherichia coli is both a highly prevalent commensal and a major opportunistic pathogen causing bloodstream infections (BSI). A systematic analysis characterizing the genomic determinants of extra-intestinal pathogenic vs. commensal isola…
View article: Dissemination of IncI plasmid encoding<i>bla</i><sub>CTX-M-1</sub>is not hampered by its fitness cost in the pig’s gut
Dissemination of IncI plasmid encoding<i>bla</i><sub>CTX-M-1</sub>is not hampered by its fitness cost in the pig’s gut Open
Multiresistance plasmids belonging to the IncI incompatibility group have become one of the most pervasive plasmid types in extended-spectrum beta-lactamase producing Escherichia coli of animal origin. The extent of the burden imposed on t…
View article: Do Anti-Biofilm Antibiotics Have a Place in the Treatment of Diabetic Foot Osteomyelitis?
Do Anti-Biofilm Antibiotics Have a Place in the Treatment of Diabetic Foot Osteomyelitis? Open
The choice of antibiotic regimens for use in patients presenting with diabetic foot osteomyelitis and their duration differs according to the situation. Antibiotics play a more important role in the medical option where no infected bone ha…
View article: Do Anti-Biofilm Antibiotics Have A Place in the Treatment of Diabetic Foot Osteomyelitis?
Do Anti-Biofilm Antibiotics Have A Place in the Treatment of Diabetic Foot Osteomyelitis? Open
The choice of antibiotic regimens to use in patients presenting with diabetic foot osteomyelitis and their duration differs according to the situation. Antibiotics play a more important role in the medical option where no infected bone has…
View article: The bacterial genetic determinants of<i>Escherichia coli</i>capacity to cause bloodstream infections in humans
The bacterial genetic determinants of<i>Escherichia coli</i>capacity to cause bloodstream infections in humans Open
Escherichia coli is both a highly prevalent commensal and a major opportunistic pathogen causing bloodstream infections (BSI). A systematic analysis characterizing the genomic determinants of extra-intestinal pathogenic vs. commensal isola…
View article: Systemic sclerosis associated interstitial lung disease: a survey of current practices in France
Systemic sclerosis associated interstitial lung disease: a survey of current practices in France Open
Background: Interstitial lung disease (ILD) is the leading cause of mortality in systemic sclerosis (SSc). Objective: We performed an overview of the diagnostic approaches, follow-up and treatment strategies used in France for the manageme…
View article: MiniBioReactor Array (MBRA) <i>in vitro</i> gut model: a reliable system to study microbiota-dependent response to antibiotic treatment
MiniBioReactor Array (MBRA) <i>in vitro</i> gut model: a reliable system to study microbiota-dependent response to antibiotic treatment Open
Background Antimicrobial drugs are mostly studied for their impact on emergence of bacterial antibiotic resistance, but their impact on the gut microbiota is also of tremendous interest. In vitro gut models are important tools to study suc…
View article: A Microbiota-Dependent Response to Anticancer Treatment in an In Vitro Human Microbiota Model: A Pilot Study With Hydroxycarbamide and Daunorubicin
A Microbiota-Dependent Response to Anticancer Treatment in an In Vitro Human Microbiota Model: A Pilot Study With Hydroxycarbamide and Daunorubicin Open
Background Anticancer drug efficacy is linked to the gut microbiota’s composition, and there is a dire need to better understand these interactions for personalized medicine. In vitro microbiota models are promising tools for studies requi…
View article: Severe SARS‐CoV‐2 patients develop a higher specific T‐cell response
Severe SARS‐CoV‐2 patients develop a higher specific T‐cell response Open
Objectives Assessment of the adaptive immune response against severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is crucial for studying long‐term immunity and vaccine strategies. We quantified IFNγ‐secreting T cells reactive aga…
View article: Airway Mucus Restricts Neisseria meningitidis Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation
Airway Mucus Restricts Neisseria meningitidis Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation Open
N. meningitidis is transmitted from person to person by aerosol droplets produced by breathing, talking, or coughing or by direct contact with a contaminated fluid. The natural reservoir of N. meningitidis is the human nasopharynx mucosa, …
View article: Advantage of the F2:A1:B- IncF Pandemic Plasmid over IncC Plasmids in <i>In Vitro</i> Acquisition and Evolution of <i>bla</i> <sub>CTX-M</sub> Gene-Bearing Plasmids in Escherichia coli
Advantage of the F2:A1:B- IncF Pandemic Plasmid over IncC Plasmids in <i>In Vitro</i> Acquisition and Evolution of <i>bla</i> <sub>CTX-M</sub> Gene-Bearing Plasmids in Escherichia coli Open
Despite a fitness cost imposed on bacterial hosts, large conjugative plasmids play a key role in the diffusion of resistance determinants, such as CTX-M extended-spectrum β-lactamases. Among the large conjugative plasmids, IncF plasmids ar…
View article: Air-interfaced colonization model suggests a commensal-like interaction of<i>Neisseria meningitidis</i>with the epithelium, which benefit from colonization by<i>Streptococcus mitis</i>
Air-interfaced colonization model suggests a commensal-like interaction of<i>Neisseria meningitidis</i>with the epithelium, which benefit from colonization by<i>Streptococcus mitis</i> Open
Neisseria meningitidis is an inhabitant of the nasopharynx, from which it is transmitted from person to person or disseminates in the blood and becomes a harmful pathogen. In this work, we addressed the colonization of the nasopharyngeal n…
View article: Acute toxoplasmosis in an immunocompetent traveller to Senegal
Acute toxoplasmosis in an immunocompetent traveller to Senegal Open
International audience
View article: Genetic and neurodevelopmental spectrum of <i>SYNGAP1</i>-associated intellectual disability and epilepsy
Genetic and neurodevelopmental spectrum of <i>SYNGAP1</i>-associated intellectual disability and epilepsy Open
Objective We aimed to delineate the neurodevelopmental spectrum associated with SYNGAP1 mutations and to investigate genotype–phenotype correlations. Methods We sequenced the exome or screened the exons of SYNGAP1 in a total of 251 patient…