David Bernardo
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View article: High-Dimensional Immune Profiling of Human Retinal Detachment Samples Using Spectral Flow Cytometry: A Protocol for Intraocular Immunotyping
High-Dimensional Immune Profiling of Human Retinal Detachment Samples Using Spectral Flow Cytometry: A Protocol for Intraocular Immunotyping Open
Retinal detachment (RD) disrupts the eye’s immune-privileged status, causing a local inflammatory response that contributes to adverse clinical outcomes, including proliferative vitreoretinopathy and suboptimal visual recovery. Comprehensi…
View article: Fungal‐Bacterial Dysbiosis in IBD: Microbial Biomarkers of Disease Activity
Fungal‐Bacterial Dysbiosis in IBD: Microbial Biomarkers of Disease Activity Open
Inflammatory bowel disease is associated with complex changes in the intestinal microbiota. While bacterial dysbiosis has been widely studied, the role of fungal communities and their interaction with bacteria remains less explored. This s…
View article: Soluble Oncoimmunome Signatures Predict Muscle Mass Response to Enriched Immunonutrition in Cancer Patients: Subanalysis of a Multicenter Randomized Clinical Trial
Soluble Oncoimmunome Signatures Predict Muscle Mass Response to Enriched Immunonutrition in Cancer Patients: Subanalysis of a Multicenter Randomized Clinical Trial Open
Background/Objectives: Enriched oral nutritional supplementation (ONS) has been shown to increase muscle mass in cancer patients. This study aims to identify the immunomodulatory effects and predictive biomarkers associated with this inter…
View article: High‐Dimensional Immunophenotyping of Post‐COVID‐19 and Post‐Influenza Patients Reveals Persistent and Specific Immune Signatures After Acute Respiratory Infection
High‐Dimensional Immunophenotyping of Post‐COVID‐19 and Post‐Influenza Patients Reveals Persistent and Specific Immune Signatures After Acute Respiratory Infection Open
Long‐term consequences of SARS‐CoV‐2 infection are unknown since recovered individuals can experience symptoms and latent viral reactivation for months. Indeed, acute post‐infection sequelae have also been observed in other respiratory vir…
View article: Study on the diagnostic role of exosome-derived miRNAs in postoperative septic shock and non-septic shock patients
Study on the diagnostic role of exosome-derived miRNAs in postoperative septic shock and non-septic shock patients Open
This study highlights extracellular vesicle-derived miRNAs as promising biomarkers for differentiating septic from non-septic shock. The identified three-miRNA signature has significant potential to enhance septic shock diagnosis, thereby …
View article: Pre-Treatment Peripheral Blood Parameters as Prognostic Biomarkers in Cancer Patients Receiving Immune Checkpoint Inhibitors
Pre-Treatment Peripheral Blood Parameters as Prognostic Biomarkers in Cancer Patients Receiving Immune Checkpoint Inhibitors Open
Background: Immune checkpoint inhibitors have significantly improved outcomes in select cancers; however, not all patients respond to these therapies, and the duration of the response varies among responders. Markers predictive of the resp…
View article: Proteomic Profiling of Extracellular Vesicles in Inflammatory Bowel Diseases
Proteomic Profiling of Extracellular Vesicles in Inflammatory Bowel Diseases Open
The proteomic analysis of serum extracellular vesicles (EVs) could be a useful tool for studying the pathophysiology of Crohn’s disease (CD) and ulcerative colitis (UC), as well as for biomarker discovery. To characterize the proteomic com…
View article: Restoration of the lamina propria duodenal immune infiltrate in gluten-free diet treated celiac patients despite persistent villous atrophy
Restoration of the lamina propria duodenal immune infiltrate in gluten-free diet treated celiac patients despite persistent villous atrophy Open
Introduction Although celiac disease (CD) current and only treatment is a life-long strict gluten-free diet (GFD), some patients suffer from persistent duodenal lesions despite years into the diet. Hence, we aimed to study the effect that …
View article: P0020 Type 1 and CD103+ type 2 conventional dendritic cells are decreased in patients with active ulcerative colitis but not with Crohn’s disease.
P0020 Type 1 and CD103+ type 2 conventional dendritic cells are decreased in patients with active ulcerative colitis but not with Crohn’s disease. Open
Background Human intestinal conventional dendritic cells (cDC) maintain the mechanisms of immune tolerance in health. However, their specific contribution to the development of inflammatory bowel disease (IBD), including ulcerative colitis…
View article: Unbiased immunome characterisation correlates with COVID-19 mRNA vaccine failure in immunocompromised adults
Unbiased immunome characterisation correlates with COVID-19 mRNA vaccine failure in immunocompromised adults Open
Introduction Coronavirus disease 2019 (COVID-19) affects the population unequally, with a greater impact on older and immunosuppressed people. Methods Hence, we performed a prospective experimental cohort study to characterise the effect o…
View article: Biological variability of human intraepithelial lymphocytes throughout the human gastrointestinal tract in health and coeliac disease
Biological variability of human intraepithelial lymphocytes throughout the human gastrointestinal tract in health and coeliac disease Open
Background Intraepithelial lymphocytes are the first line of defence of the human intestinal immune system. Besides, their composition is altered on patients with coeliac disease (CD), so they are considered as biomarkers with utility on t…
View article: miRNome Profiling of Extracellular Vesicles in Patients With Severe COVID-19 and Identification of Predictors of Mortality
miRNome Profiling of Extracellular Vesicles in Patients With Severe COVID-19 and Identification of Predictors of Mortality Open
Background Extracellular vesicles (EVs), containing microRNAs (miRNAs) and other molecules, play a central role in intercellular communication, especially in viral infections caused by SARS-CoV-2. This study explores the miRNA profiles in …
View article: State-of-the-art cytometry in the search of novel biomarkers in digestive cancers
State-of-the-art cytometry in the search of novel biomarkers in digestive cancers Open
Despite that colorectal and liver cancer are among the most prevalent tumours in the world, the identification of non-invasive biomarkers to aid on their diagnose and subsequent prognosis is a current unmet need that would diminish both th…
View article: Circulating immunome fingerprint in eosinophilic esophagitis is associated with clinical response to proton pump inhibitor treatment
Circulating immunome fingerprint in eosinophilic esophagitis is associated with clinical response to proton pump inhibitor treatment Open
Objectives The aim of the study was to characterize the circulating immunome of patients with EoE before and after proton pump inhibitor (PPI) treatment in order to identify potential non-invasive biomarkers of treatment response. Methods …
View article: Circulating immunome fingerprint in eosinophilic esophagitis is associated with clinical response to proton pump inhibitor treatment
Circulating immunome fingerprint in eosinophilic esophagitis is associated with clinical response to proton pump inhibitor treatment Open
Objectives The aim of the study was to characterize the circulating immunome of patients with EoE before and after proton pump inhibitor (PPI) treatment in order to identify potential non-invasive biomarkers of treatment response. Methods …
View article: A genome-wide association study for survival from a multi-centre European study identified variants associated with COVID-19 risk of death
A genome-wide association study for survival from a multi-centre European study identified variants associated with COVID-19 risk of death Open
The clinical manifestations of SARS-CoV-2 infection vary widely among patients, from asymptomatic to life-threatening. Host genetics is one of the factors that contributes to this variability as a previous case-control genome-wide associat…
View article: Genetic Architecture of Ischaemic Strokes after COVID-19 Shows Similarities with Large Vessel Strokes
Genetic Architecture of Ischaemic Strokes after COVID-19 Shows Similarities with Large Vessel Strokes Open
We aimed to analyse whether patients with ischaemic stroke (IS) occurring within eight days after the onset of COVID-19 (IS-COV) are associated with a specific aetiology of IS. We used SUPERGNOVA to identify genome regions that correlate b…
View article: Serum and Urine Metabolomic Profiling of Newly Diagnosed Treatment-Naïve Inflammatory Bowel Disease Patients
Serum and Urine Metabolomic Profiling of Newly Diagnosed Treatment-Naïve Inflammatory Bowel Disease Patients Open
Background and Aims Inflammatory bowel disease (IBD) is a prevalent chronic noncurable disease associated with profound metabolic changes. The discovery of novel molecular indicators for unraveling IBD etiopathogenesis and the diagnosis an…
View article: Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress
Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress Open
Introduction The Unfolded Protein Response, a mechanism triggered by the cell in response to Endoplasmic reticulum stress, is linked to inflammatory responses. Our aim was to identify novel Unfolded Protein Response-mechanisms that might b…
View article: Unbiased spectral cytometry immunome characterization predicts COVID-19 mRNA vaccine failure in older adults and patients with lymphoid malignancies
Unbiased spectral cytometry immunome characterization predicts COVID-19 mRNA vaccine failure in older adults and patients with lymphoid malignancies Open
COVID-19 affects the population unequally with a higher impact on aged and immunosuppressed people. Hence, we assessed the effect of SARS-CoV-2 vaccination in immune compromised patients (older adults and oncohematologic patients), compare…
View article: Top-of-the-art cytometry as a novel tool to aid in lung cancer immunotherapy
Top-of-the-art cytometry as a novel tool to aid in lung cancer immunotherapy Open
Non-small cell lung cancer (NSCLC) is perhaps the cancer type that better exemplifies the transition to precision medicine for cancer. Indeed, around 15% of patients with advanced NSCLC display genomic alterations in EGFR, ALK, ROS1, BRAF …
View article: Telepsychiatry: the use of technology to improve access to mental health care
Telepsychiatry: the use of technology to improve access to mental health care Open
Introduction Telepsychiatry (TP) uses communication technology to provide psychiatric consultation to patients unable to reach consultation services. Due to COVID-19 outbreak, many mental health services implemented TP. The University of M…
View article: P044 Ex vivo effects of infliximab on the long non-coding RNAs expression levels in Crohn′s disease
P044 Ex vivo effects of infliximab on the long non-coding RNAs expression levels in Crohn′s disease Open
Background In recent years, evidence shows that long non-coding RNAs (lncRNAs) are key regulators of gene transcription and play important roles in the pathogenesis of inflammatory bowel diseases. LncRNA are involved in regulation of intes…
View article: P058 Immunological effects of hepatitis B virus vaccination in inflammatory bowel disease patients
P058 Immunological effects of hepatitis B virus vaccination in inflammatory bowel disease patients Open
Background The response rate to hepatitis B virus (HBV) vaccination in the general population is about 90%. However, the response rate to the vaccine in patients with inflammatory bowel disease (IBD) is significantly lower. IBD is a multif…
View article: I-FABP, abdominal distension and Hydrogen concentration after lactitol SIBO test
I-FABP, abdominal distension and Hydrogen concentration after lactitol SIBO test Open
Breath tests with glucose, lactulose or lactitol are useful for diagnosis of small intestinal bacterial overgrowth (SIBO). Nevertheless, they have suboptimal sensitivity and specificity and, indeed, are positive in a considerable number of…
View article: MIF is a common genetic determinant of COVID-19 symptomatic infection and severity
MIF is a common genetic determinant of COVID-19 symptomatic infection and severity Open
Background Genetic predisposition to coronavirus disease 2019 (COVID-19) may contribute to its morbidity and mortality. Because cytokines play an important role in multiple phases of infection, we examined whether commonly occurring, funct…