Bert Poolman
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View article: Spatiotemporal organization of the bacterial cytoplasm
Spatiotemporal organization of the bacterial cytoplasm Open
The bacterial cytoplasm is a dynamic and crowded environment where the majority of the molecules of the cell can (transiently) interact and react. Here, we focus on the spatiotemporal organization of the bacterial cytoplasm, with emphasis …
View article: Condition-dependent, amorphous protein agglomerates control cytoplasmic rheology
Condition-dependent, amorphous protein agglomerates control cytoplasmic rheology Open
Summary Molecular crowding in the bacterial cytoplasm restricts the diffusion of large molecules, impacting cellular processes. However, how nutrient availability influences cytoplasmic rheology is not well understood. With single-particle…
View article: Structural and functional implications of in vivo phase separation of membrane protein in Escherichia coli
Structural and functional implications of in vivo phase separation of membrane protein in Escherichia coli Open
Liquid-liquid phase-separation (LLPS) controls protein activity and dynamically organizes (macro)molecules in living systems without the need for membrane-bound compartments. Biomolecular condensates of water-soluble proteins have extensiv…
View article: The <i>Saccharomyces cerevisiae</i> amino acid transporter Lyp1 has a broad substrate spectrum
The <i>Saccharomyces cerevisiae</i> amino acid transporter Lyp1 has a broad substrate spectrum Open
The main mediators for the amino acid uptake in Saccharomyces cerevisiae are the permeases belonging to the yeast amino acid transporter family. Recently, we discovered that members of this family support growth on more amino acids than pr…
View article: Balancing Permeability and Stability: A Study of Hybrid Membranes for Synthetic Cells Using Lipids and PBd-<i>b</i>-PEO Block Copolymers
Balancing Permeability and Stability: A Study of Hybrid Membranes for Synthetic Cells Using Lipids and PBd-<i>b</i>-PEO Block Copolymers Open
We have synthesized hybrid membranes composed of amphiphilic block copolymers, polybutadiene-poly(ethylene oxide) [PBd-b-PEO], with different lengths [PBd22-PEO14 and PBd11-PEO8] and mixtures of phospholipids (DOPC:DOPG:DOPE 50:25:25 mol %…
View article: Chemiosmotic nutrient transport in synthetic cells powered by electrogenic antiport coupled to decarboxylation
Chemiosmotic nutrient transport in synthetic cells powered by electrogenic antiport coupled to decarboxylation Open
Cellular homeostasis depends on the supply of metabolic energy in the form of ATP and electrochemical ion gradients. The construction of synthetic cells requires a constant supply of energy to drive membrane transport and metabolism. Here,…
View article: Exploring the Ligand Binding and Conformational Dynamics of the Substrate-Binding Domain 1 of the ABC Transporter GlnPQ
Exploring the Ligand Binding and Conformational Dynamics of the Substrate-Binding Domain 1 of the ABC Transporter GlnPQ Open
The adenosine triphosphate (ATP)-binding cassette (ABC) importer GlnPQ from Lactococcus lactis has two sequential covalently linked substrate-binding domains (SBDs), which capture the substrates and deliver them to the translocon. The two …
View article: Fitness landscape of substrate-adaptive mutations in evolved amino acid-polyamine-organocation transporters
Fitness landscape of substrate-adaptive mutations in evolved amino acid-polyamine-organocation transporters Open
The emergence of new protein functions is crucial for the evolution of organisms. This process has been extensively researched for soluble enzymes, but it is largely unexplored for membrane transporters, even though the ability to acquire …
View article: The Structure and Function of the Bacterial Osmotically Inducible Protein Y
The Structure and Function of the Bacterial Osmotically Inducible Protein Y Open
The ability to adapt to osmotically diverse and fluctuating environments is critical to the survival and resilience of bacteria that colonize the human gut and urinary tract. Environmental stress often provides cross-protection against oth…
View article: Reviewer #1 (Public Review): Fitness landscape of substrate-adaptive mutations in evolved APC transporters
Reviewer #1 (Public Review): Fitness landscape of substrate-adaptive mutations in evolved APC transporters Open
The emergence of new protein functions is crucial for the evolution of organisms. This process has been extensively researched for soluble enzymes, but it is largely unexplored for membrane transporters, even though the ability to acquire …
View article: Bacterial cell volume regulation and the importance of cyclic di-AMP
Bacterial cell volume regulation and the importance of cyclic di-AMP Open
SUMMARY Nucleotide-derived second messengers are present in all domains of life. In prokaryotes, most of their functionality is associated with general lifestyle and metabolic adaptations, often in response to environmental fluctuations of…
View article: Reviewer #2 (Public Review): Fitness landscape of substrate-adaptive mutations in evolved APC transporters
Reviewer #2 (Public Review): Fitness landscape of substrate-adaptive mutations in evolved APC transporters Open
The emergence of new protein functions is crucial for the evolution of organisms. This process has been extensively researched for soluble enzymes, but it is largely unexplored for membrane transporters, even though the ability to acquire …
View article: Fitness landscape of substrate-adaptive mutations in evolved APC transporters
Fitness landscape of substrate-adaptive mutations in evolved APC transporters Open
The emergence of new protein functions is crucial for the evolution of organisms. This process has been extensively researched for soluble enzymes, but it is largely unexplored for membrane transporters, even though the ability to acquire …
View article: Reviewer #3 (Public Review): Fitness landscape of substrate-adaptive mutations in evolved APC transporters
Reviewer #3 (Public Review): Fitness landscape of substrate-adaptive mutations in evolved APC transporters Open
The emergence of new protein functions is crucial for the evolution of organisms. This process has been extensively researched for soluble enzymes, but it is largely unexplored for membrane transporters, even though the ability to acquire …
View article: Author response: Fitness landscape of substrate-adaptive mutations in evolved APC transporters
Author response: Fitness landscape of substrate-adaptive mutations in evolved APC transporters Open
The emergence of new protein functions is crucial for the evolution of organisms.This process has been extensively researched for soluble enzymes, but it is largely unexplored for membrane transporters, even though the ability to acquire n…
View article: The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact
The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact Open
Bacteria utilize various strategies to prevent internal dehydration during hypertonic stress. A common approach to countering the effects of the stress is to import compatible solutes such as glycine betaine, leading to simultaneous passiv…
View article: Dynamic structure of E. coli cytoplasm: supramolecular complexes and cell aging impact spatial distribution and mobility of proteins
Dynamic structure of E. coli cytoplasm: supramolecular complexes and cell aging impact spatial distribution and mobility of proteins Open
Protein diffusion is a critical factor governing the functioning and organization of a cell’s cytoplasm. In this study, we investigate the influence of (poly)ribosome distribution, cell aging, protein aggregation, and biomolecular condensa…
View article: A sustainable chemiosmotic strategy for driving solute transport in synthetic cells
A sustainable chemiosmotic strategy for driving solute transport in synthetic cells Open
Cellular homeostasis requires sustained provision of metabolic energy in the form of ATP and electrochemical ion gradients. Primary and secondary active transporters are prominent consumers of cellular energy, and couple ATP hydrolysis and…
View article: Synthetic Vesicles for Sustainable Energy Recycling and Delivery of Building Blocks for Lipid Biosynthesis
Synthetic Vesicles for Sustainable Energy Recycling and Delivery of Building Blocks for Lipid Biosynthesis Open
ATP is a universal energy currency that is essential for life. l-Arginine degradation via deamination is an elegant way to generate ATP in synthetic cells, which is currently limited by a slow l-arginine/l-ornithine exchange. We are now im…
View article: Reviewer #1 (Public Review): The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact
Reviewer #1 (Public Review): The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact Open
Bacteria utilize various strategies to prevent internal dehydration during hypertonic stress. A common approach to countering the effects of the stress is to import compatible solutes such as glycine betaine, leading to simultaneous passiv…
View article: Author response: The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact
Author response: The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact Open
Bacteria utilize various strategies to prevent internal dehydration during hypertonic stress. A common approach to countering the effects of the stress is to import compatible solutes such as glycine betaine, leading to simultaneous passiv…
View article: The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact
The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact Open
Bacteria utilize various strategies to prevent internal dehydration during hypertonic stress. A common approach to countering the effects of the stress is to import compatible solutes such as glycine betaine, leading to simultaneous passiv…
View article: Reviewer #2 (Public Review): The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact
Reviewer #2 (Public Review): The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact Open
Bacteria utilize various strategies to prevent internal dehydration during hypertonic stress. A common approach to countering the effects of the stress is to import compatible solutes such as glycine betaine, leading to simultaneous passiv…
View article: Fitness landscape of substrate-adaptive mutations in evolved APC transporters
Fitness landscape of substrate-adaptive mutations in evolved APC transporters Open
Growth rate calculations: Single colonies of S. cerevisiae Δ10AA pADHXC3GH-GOI were inoculated in YB media supplemented with 4 mm NH4+ and 0.1 mg/ml ampicillin, and grown until late logarithmic phase. The cultures were pelleted at 750 × g …