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View article: Recommendations for Studying <i>In Situ</i> Extracellular Vesicles From Solid Tissue
Recommendations for Studying <i>In Situ</i> Extracellular Vesicles From Solid Tissue Open
Solid tissue‐derived extracellular vesicles (ST‐EVs) are extracellular vesicles (EVs) separated directly from solid tissues of both vertebrates and invertebrates. ST‐EVs provide a physiologically relevant snapshot of tissue‐specific molecu…
View article: High-Purity Enrichment of Extracellular Vesicles from Diverse Sources by Conventional and Image-Based Fluorescence Activated Cell Sorters for Robust Downstream Applications
High-Purity Enrichment of Extracellular Vesicles from Diverse Sources by Conventional and Image-Based Fluorescence Activated Cell Sorters for Robust Downstream Applications Open
Separating and enriching specific extracellular vesicle (EV) subpopulations from the broader EV pool present in tissues and blood is crucial for understanding their role in physiological and pathological conditions. However, high-purity en…
View article: Binding of extracellular vesicles to stretched von Willebrand factor promotes platelet activation
Binding of extracellular vesicles to stretched von Willebrand factor promotes platelet activation Open
Von Willebrand factor (vWF), promoting platelet aggregation in various diseases such as COVID-19, malaria and cancer, is a huge multimeric glycoprotein. This extraordinary size makes vWF a unique shear stress sensing molecule. Below a crit…
View article: Extracellular Vesicles Carrying Tenascin-C are Clinical Biomarkers and Improve Tumor-Derived DNA Analysis in Glioblastoma Patients
Extracellular Vesicles Carrying Tenascin-C are Clinical Biomarkers and Improve Tumor-Derived DNA Analysis in Glioblastoma Patients Open
Extracellular vesicles (EVs) act as carriers of biological information from tumors to the bloodstream, enabling the detection of circulating tumor material and tracking of disease progression. This is particularly crucial in glioblastoma, …
View article: Efficient enzyme‐free isolation of brain‐derived extracellular vesicles
Efficient enzyme‐free isolation of brain‐derived extracellular vesicles Open
Extracellular vesicles (EVs) have gained significant attention as pathology mediators and potential diagnostic tools for neurodegenerative diseases. However, isolation of brain‐derived EVs (BDEVs) from tissue remains challenging, often inv…
View article: Cleavage site-directed antibodies reveal the prion protein in humans is shed by ADAM10 at Y226 and associates with misfolded protein deposits in neurodegenerative diseases
Cleavage site-directed antibodies reveal the prion protein in humans is shed by ADAM10 at Y226 and associates with misfolded protein deposits in neurodegenerative diseases Open
Proteolytic cell surface release (‘shedding’) of the prion protein (PrP), a broadly expressed GPI-anchored glycoprotein, by the metalloprotease ADAM10 impacts on neurodegenerative and other diseases in animal and in vitro models. Recent st…
View article: Efficient enzyme-free isolation of brain-derived extracellular vesicles
Efficient enzyme-free isolation of brain-derived extracellular vesicles Open
Extracellular vesicles (EVs) have gained significant attention as pathology mediators and potential diagnostic tools for neurodegenerative diseases. However, isolation of brain-derived EVs (BDEVs) from tissue remains challenging, often inv…
View article: Neuroserpin and Extracellular Vesicles in Ischemic Stroke: Partners in Neuroprotection?
Neuroserpin and Extracellular Vesicles in Ischemic Stroke: Partners in Neuroprotection? Open
Ischemic stroke represents a significant global health challenge, often resulting in death or long-term disability, particularly among the elderly, where advancing age stands as the most unmodifiable risk factor. Arising from the blockage …
View article: Cleavage site-directed antibodies reveal the prion protein in humans is shed by ADAM10 at Y226 and associates with misfolded protein deposits in neurodegenerative diseases
Cleavage site-directed antibodies reveal the prion protein in humans is shed by ADAM10 at Y226 and associates with misfolded protein deposits in neurodegenerative diseases Open
Proteolytic cell surface release (‘shedding’) of the prion protein (PrP), a broadly expressed GPI-anchored glycoprotein, by the metalloprotease ADAM10 impacts on neurodegenerative and other diseases in animal and in vitro models. Recent st…
View article: Inducing prion protein shedding as a neuroprotective and regenerative approach in pathological conditions of the brain: from theory to facts.
Inducing prion protein shedding as a neuroprotective and regenerative approach in pathological conditions of the brain: from theory to facts. Open
In the last decades, the role of the prion protein (PrP) in neurodegenerative diseases has been intensively investigated, initially in prion diseases of humans (e.g., Creutzfeldt-Jakob disease) and animals (e.g., scrapie in sheep, chronic …
View article: Cystatin C loaded in brain-derived extracellular vesicles rescues synapses after ischemic insult<i>in vitro</i>and<i>in vivo</i>
Cystatin C loaded in brain-derived extracellular vesicles rescues synapses after ischemic insult<i>in vitro</i>and<i>in vivo</i> Open
Synaptic loss is an early event in the undersupplied but not yet irreversibly injured penumbra area after an ischemic stroke. Promoting synaptic preservation in this area would likely improve functional neurological recovery. In the presen…
View article: Retinal regions shape human and murine Müller cell proteome profile and functionality
Retinal regions shape human and murine Müller cell proteome profile and functionality Open
The human macula is a highly specialized retinal region with pit‐like morphology and rich in cones. How Müller cells, the principal glial cell type in the retina, are adapted to this environment is still poorly understood. We compared prot…
View article: Release of VAMP5‐positive extracellular vesicles by retinal Müller glia in vivo
Release of VAMP5‐positive extracellular vesicles by retinal Müller glia in vivo Open
Cell‐cell interactions in the central nervous system are based on the release of molecules mediating signal exchange and providing structural and trophic support through vesicular exocytosis and the formation of extracellular vesicles. The…
View article: Release of VAMP5-positive extracellular vesicles by retinal Müller glia<i>in vivo</i>
Release of VAMP5-positive extracellular vesicles by retinal Müller glia<i>in vivo</i> Open
Cell-cell interactions in the central nervous system are based on the release of molecules mediating signal exchange and providing structural and trophic support through vesicular exocytosis and the formation of extracellular vesicles. The…
View article: Anchorless risk or released benefit? An updated view on the ADAM10-mediated shedding of the prion protein
Anchorless risk or released benefit? An updated view on the ADAM10-mediated shedding of the prion protein Open
The prion protein (PrP) is a broadly expressed glycoprotein linked with a multitude of (suggested) biological and pathological implications. Some of these roles seem to be due to constitutively generated proteolytic fragments of the protei…
View article: Multiplexed mRNA analysis of brain-derived extracellular vesicles upon experimental stroke in mice reveals increased mRNA content related to inflammation and recovery processes
Multiplexed mRNA analysis of brain-derived extracellular vesicles upon experimental stroke in mice reveals increased mRNA content related to inflammation and recovery processes Open
Extracellular vesicles (EVs) are lipid bilayer enclosed structures that not only represent a newly discovered means for cell-to-cell communication but may also serve as promising disease biomarkers and therapeutic tools. Apart from protein…
View article: Ligands binding to the prion protein induce its proteolytic release with therapeutic potential in neurodegenerative proteinopathies
Ligands binding to the prion protein induce its proteolytic release with therapeutic potential in neurodegenerative proteinopathies Open
Ligand-stimulated enzymatic release or cellular degradation of PrP may interfere with neurodegeneration-associated toxicity.
View article: CD73-mediated adenosine production by CD8 T cell-derived extracellular vesicles constitutes an intrinsic mechanism of immune suppression
CD73-mediated adenosine production by CD8 T cell-derived extracellular vesicles constitutes an intrinsic mechanism of immune suppression Open
Immune cells at sites of inflammation are continuously activated by local antigens and cytokines, and regulatory mechanisms must be enacted to control inflammation. The stepwise hydrolysis of extracellular ATP by ectonucleotidases CD39 and…
View article: Ligands binding to the cellular prion protein induce its protective proteolytic release with therapeutic potential in neurodegenerative proteinopathies
Ligands binding to the cellular prion protein induce its protective proteolytic release with therapeutic potential in neurodegenerative proteinopathies Open
The cellular prion protein (PrP C ) is a central player in neurodegenerative diseases caused by protein misfolding, such as prion diseases or Alzheimer’s disease (AD). Expression levels of this GPI-anchored glycoprotein, especially at the …
View article: Brain-Derived Extracellular Vesicles in Health and Disease: A Methodological Perspective
Brain-Derived Extracellular Vesicles in Health and Disease: A Methodological Perspective Open
Extracellular vesicles (EVs) are double membrane structures released by presumably all cell types that transport and deliver lipids, proteins, and genetic material to near or distant recipient cells, thereby affecting their phenotype. The …
View article: Additional file 7 of Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease
Additional file 7 of Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease Open
Additional file 7. The additional file contains spreadsheets with averaged normalized mass spectrometric data after removal of candidates found in beads only controls (unique peptide counts), for the interactors of high-density prion prote…
View article: Additional file 3 of Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease
Additional file 3 of Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease Open
Additional file 3. The spreadsheet contains the differential expression of cytoskeletal and cytoskeletal-associated proteins from the frontal cortex tissue lysates of Con, spAD, rpAD, SVD, DFTL, rDLB and DLB patients assessed using the SWA…
View article: Additional file 6 of Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease
Additional file 6 of Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease Open
Additional file 6. The spreadsheet contains the raw proteomics data (unique peptide counts data) of HDFs, and subsequent normalized data along with the results of pairwise statistical testing (Welch’s t-test).
View article: Additional file 4 of Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease
Additional file 4 of Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease Open
Additional file 4. The spreadsheet gives the average peptide counts and SEM of the cytoskeletal and cytoskeletal-associated proteins from the HDF pools from frontal cortex lysates of control brains and those other neurodegenerative samples…
View article: Additional file 5 of Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease
Additional file 5 of Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease Open
Additional file 5. The spreadsheet contains the raw global proteomics data (SWATH-MS values) of Con, spAD, rpAD, DLB, rDLB, DFTL, and SVD. Subsequent normalized data along with the results of pairwise statistical testing (Welch’s t-test).