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View article: Dual CRISPRi-seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle
Dual CRISPRi-seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle Open
Uncovering genotype-phenotype relationships is hampered by genetic redundancy. For example, most genes in Streptococcus pneumoniae are non-essential under laboratory conditions. A powerful approach to unravel genetic redundancy is by ident…
View article: Dual CRISPRi-Seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle
Dual CRISPRi-Seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle Open
A major goal in biology is to uncover the relationship between genotype and phenotype. However, identifying gene function is often hampered by genetic redundancy. For example, under standard laboratory conditions, three-quarters of the gen…
View article: CRISPRi–TnSeq maps genome-wide interactions between essential and non-essential genes in bacteria
CRISPRi–TnSeq maps genome-wide interactions between essential and non-essential genes in bacteria Open
View article: <i>In vivo</i>tests of the<i>E. coli</i>TonB system working model—interaction of ExbB with unknown proteins, identification of TonB-ExbD transmembrane heterodimers and PMF-dependent ExbD structures
<i>In vivo</i>tests of the<i>E. coli</i>TonB system working model—interaction of ExbB with unknown proteins, identification of TonB-ExbD transmembrane heterodimers and PMF-dependent ExbD structures Open
The TonB system of Escherichia coli resolves the dilemma posed by its outer membrane that protects it from a variety of external threats, but also constitutes a diffusion barrier to nutrient uptake. Our working model involves interactions …
View article: The intrinsic macrolide resistome of <i>Escherichia coli</i>
The intrinsic macrolide resistome of <i>Escherichia coli</i> Open
Intrinsic resistance to macrolides in Gram-negative bacteria is primarily attributed to the low permeability of the outer membrane, though the underlying genetic and molecular mechanisms remain to be fully elucidated. Here, we used transpo…
View article: CRISPRi-TnSeq: A genome-wide high-throughput tool for bacterial essential-nonessential genetic interaction mapping
CRISPRi-TnSeq: A genome-wide high-throughput tool for bacterial essential-nonessential genetic interaction mapping Open
Genetic interaction networks can help identify functional connections between genes and pathways, which can be leveraged to establish (new) gene function, drug targets, and fill pathway gaps. Since there is no optimal tool that can map gen…
View article: LptD depletion disrupts morphological homeostasis and upregulates carbohydrate metabolism in <i>Escherichia coli</i>
LptD depletion disrupts morphological homeostasis and upregulates carbohydrate metabolism in <i>Escherichia coli</i> Open
In a previous in silico study, we identified an essential outer membrane protein (LptD) as an attractive target for development of novel antibiotics. Here, we characterized the effects of LptD depletion on Escherichia coli physiology and m…
View article: P159: Variants in cohesin release factors WAPL, PDS5A, and PDS5B define a new class of cohesinopathies*
P159: Variants in cohesin release factors WAPL, PDS5A, and PDS5B define a new class of cohesinopathies* Open
View article: Studies on the<i>Escherichia coli</i>ExbD Transmembrane Domain, Residue L132, and an Inhibitory Cyclic Peptide
Studies on the<i>Escherichia coli</i>ExbD Transmembrane Domain, Residue L132, and an Inhibitory Cyclic Peptide Open
The TonB system of Escherichia coli uses the cytoplasmic membrane protonmotive force (PMF) to energize active transport of nutrients across the otherwise unenergized outer membrane. Because it overcomes limitations for nutrient diffusion t…
View article: A genome-wide atlas of antibiotic susceptibility targets and pathways to tolerance
A genome-wide atlas of antibiotic susceptibility targets and pathways to tolerance Open
Detailed knowledge on how bacteria evade antibiotics and eventually develop resistance could open avenues for novel therapeutics and diagnostics. It is thereby key to develop a comprehensive genome-wide understanding of how bacteria proces…
View article: Topical niclosamide (ATx201) reduces <i>Staphylococcus aureus</i> colonization and increases Shannon diversity of the skin microbiome in atopic dermatitis patients in a randomized, double‐blind, placebo‐controlled Phase 2 trial
Topical niclosamide (ATx201) reduces <i>Staphylococcus aureus</i> colonization and increases Shannon diversity of the skin microbiome in atopic dermatitis patients in a randomized, double‐blind, placebo‐controlled Phase 2 trial Open
Background In patients with atopic dermatitis (AD), Staphylococcus aureus frequently colonizes lesions and is hypothesized to be linked to disease severity and progression. Treatments that reduce S. aureus colonization without significantl…
View article: A genome-wide atlas of antibiotic susceptibility targets and pathways to tolerance
A genome-wide atlas of antibiotic susceptibility targets and pathways to tolerance Open
Detailed knowledge on how bacteria evade antibiotics and eventually develop resistance could open avenues for novel therapeutics and diagnostics. It is thereby key to develop a comprehensive genome-wide understanding of how bacteria proces…
View article: Insight Into the Anti-staphylococcal Activity of JBC 1847 at Sub-Inhibitory Concentration
Insight Into the Anti-staphylococcal Activity of JBC 1847 at Sub-Inhibitory Concentration Open
Multidrug-resistant pathogens constitute a serious global issue and, therefore, novel antimicrobials with new modes of action are urgently needed. Here, we investigated the effect of a phenothiazine derivative (JBC 1847) with high antimicr…
View article: In Silico Prediction and Prioritization of Novel Selective Antimicrobial Drug Targets in Escherichia coli
In Silico Prediction and Prioritization of Novel Selective Antimicrobial Drug Targets in Escherichia coli Open
Novel antimicrobials interfering with pathogen-specific targets can minimize the risk of perturbations of the gut microbiota (dysbiosis) during therapy. We employed an in silico approach to identify essential proteins in Escherichia coli t…
View article: In Silico Prediction and Prioritisation of Novel Selective Antimicrobial Drug Targets in Escherichia Coli
In Silico Prediction and Prioritisation of Novel Selective Antimicrobial Drug Targets in Escherichia Coli Open
Treatment of infections caused by Escherichia coli and other Enterobacteriaceae often requires broad-spectrum antimicrobials, which cause perturbations of the gut microbiota (dysbiosis). Novel antimicrobial drugs interfering with pathogen-…
View article: Carprofen-induced depletion of proton motive force reverses TetK-mediated doxycycline resistance in methicillin-resistant Staphylococcus pseudintermedius
Carprofen-induced depletion of proton motive force reverses TetK-mediated doxycycline resistance in methicillin-resistant Staphylococcus pseudintermedius Open
View article: Repurposing Azithromycin and Rifampicin Against Gram-Negative Pathogens by Combination With Peptidomimetics
Repurposing Azithromycin and Rifampicin Against Gram-Negative Pathogens by Combination With Peptidomimetics Open
Synthetic peptidomimetics may be designed to mimic functions of antimicrobial peptides, including potentiation of antibiotics, yet possessing improved pharmacological properties. Pairwise screening of 42 synthetic peptidomimetics combined …
View article: Publisher Correction: Characterization, mechanism of action and optimization of activity of a novel peptide-peptoid hybrid against bacterial pathogens involved in canine skin infections
Publisher Correction: Characterization, mechanism of action and optimization of activity of a novel peptide-peptoid hybrid against bacterial pathogens involved in canine skin infections Open
View article: Structure–Activity Study, Characterization, and Mechanism of Action of an Antimicrobial Peptoid D2 and Its d- and l-Peptide Analogues
Structure–Activity Study, Characterization, and Mechanism of Action of an Antimicrobial Peptoid D2 and Its d- and l-Peptide Analogues Open
Methicillin-resistant Staphylococcus pseudintermedius (MRSP) constitutes an emerging health problem for companion animals in veterinary medicine. Therefore, discovery of novel antimicrobial agents for treatment of Staphylococcus-associated…
View article: Fate of CMY-2-Encoding Plasmids Introduced into the Human Fecal Microbiota by Exogenous <i>Escherichia coli</i>
Fate of CMY-2-Encoding Plasmids Introduced into the Human Fecal Microbiota by Exogenous <i>Escherichia coli</i> Open
The gut is a hot spot for transfer of antibiotic resistance genes from ingested exogenous bacteria to the indigenous microbiota. The objective of this study was to determine the fate of two nearly identical bla CMY-2 -harboring plasmids in…
View article: Characterization, mechanism of action and optimization of activity of a novel peptide-peptoid hybrid against bacterial pathogens involved in canine skin infections
Characterization, mechanism of action and optimization of activity of a novel peptide-peptoid hybrid against bacterial pathogens involved in canine skin infections Open
Integumentary infections like pyoderma represent the main reason for antimicrobial prescription in dogs. Staphylococcus pseudintermedius and Pseudomonas aeruginosa are frequently identified in these infections, and both bacteria are challe…
View article: Repurposing azithromycin and rifampicin against Gram-negative pathogens by combination with peptide potentiators
Repurposing azithromycin and rifampicin against Gram-negative pathogens by combination with peptide potentiators Open
View article: Transcriptome analysis of extended-spectrum β-lactamase-producing Escherichia coli and methicillin-resistant Staphylococcus aureus exposed to cefotaxime
Transcriptome analysis of extended-spectrum β-lactamase-producing Escherichia coli and methicillin-resistant Staphylococcus aureus exposed to cefotaxime Open
View article: A culture-independent method for studying transfer of IncI1 plasmids from wild-type Escherichia coli in complex microbial communities
A culture-independent method for studying transfer of IncI1 plasmids from wild-type Escherichia coli in complex microbial communities Open
View article: DNA Damage Repair and Drug Efflux as Potential Targets for Reversing Low or Intermediate Ciprofloxacin Resistance in E. coli K-12
DNA Damage Repair and Drug Efflux as Potential Targets for Reversing Low or Intermediate Ciprofloxacin Resistance in E. coli K-12 Open
Ciprofloxacin is a potent antibacterial drug that is widely used in human clinical applications. As a consequence of its extensive use, resistance has emerged in almost all clinically relevant bacterial species. A mean to combat the observ…
View article: Dogs as reservoirs of<i>Escherichia coli</i>strains causing urinary tract infection in their owners
Dogs as reservoirs of<i>Escherichia coli</i>strains causing urinary tract infection in their owners Open
It is known that humans and pets living together can share the same Escherichia coli strain. In this study we assessed the role played by household pets as reservoirs of E. coli strains causing urinary tract infection (UTI) in their owners…
View article: The secondary resistome of multidrug-resistant Klebsiella pneumoniae
The secondary resistome of multidrug-resistant Klebsiella pneumoniae Open
View article: Cephem Potentiation by Inactivation of Nonessential Genes Involved in Cell Wall Biogenesis of β-Lactamase-Producing Escherichia coli
Cephem Potentiation by Inactivation of Nonessential Genes Involved in Cell Wall Biogenesis of β-Lactamase-Producing Escherichia coli Open
Reversal of antimicrobial resistance is an appealing and largely unexplored strategy in drug discovery. The objective of this study was to identify potential targets for “helper” drugs reversing cephem resistance in Escherichia coli strain…
View article: A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli
A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli Open
The envelope of Gram-negative bacteria constitutes an impenetrable barrier to numerous classes of antimicrobials. This intrinsic resistance, coupled with acquired multidrug resistance, has drastically limited the treatment options against …
View article: Antimicrobial synergy between carprofen and doxycycline against methicillin-resistant Staphylococcus pseudintermedius ST71
Antimicrobial synergy between carprofen and doxycycline against methicillin-resistant Staphylococcus pseudintermedius ST71 Open
Background: New therapeutic strategies are needed to face the rapid spread of multidrug-resistant staphylococci in veterinary medicine. The objective of this study was to identify synergies between antimicrobial and non-antimicrobial drugs…