Birgit Dreier
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View article: Selective targeting of TBXT with DARPins identifies regulatory networks and therapeutic vulnerabilities in chordoma
Selective targeting of TBXT with DARPins identifies regulatory networks and therapeutic vulnerabilities in chordoma Open
The embryonic transcription factor TBXT (brachyury) drives chordoma, a spinal neoplasm without effective drug therapies. TBXT’s regulatory network is poorly understood, and strategies to disrupt its activity for therapeutic purposes are la…
View article: Pre-assembly of biomolecular condensate seeds drives RSV replication
Pre-assembly of biomolecular condensate seeds drives RSV replication Open
During infection many RNA viruses, including respiratory syncytial virus (RSV), form specialized biomolecular condensates, inclusion bodies (IBs), where viral transcription and replication occur 1–4 . Paradoxically, high protein concentrat…
View article: Heterodimerization of staphylococcal phage φ2638A endolysin isoforms and their functional role in bacterial lysis
Heterodimerization of staphylococcal phage φ2638A endolysin isoforms and their functional role in bacterial lysis Open
Bacteriophage endolysins targeting Gram-positive bacteria typically feature a modular architecture of one or more enzymatically active domains (EADs) and cell wall binding domains (CBDs). Several endolysins also feature internal translatio…
View article: A designed ankyrin-repeat protein that targets Parkinson’s disease-associated LRRK2
A designed ankyrin-repeat protein that targets Parkinson’s disease-associated LRRK2 Open
Leucine rich repeat kinase 2 (LRRK2) is a large multidomain protein containing two catalytic domains, a kinase and a GTPase, as well as protein interactions domains, including a WD40 domain. The association of increased LRRK2 kinase activi…
View article: mRNA Display Pipeline for Protein Biosensor Construction
mRNA Display Pipeline for Protein Biosensor Construction Open
Despite the significant potential of protein biosensors, their construction remains a trial-and-error process. The most obvious approach for addressing this is to utilize modular biosensor architectures where specificity-conferring modalit…
View article: Designed Ankyrin Repeat Proteins as Actin Labels of Distinct Cytoskeletal Structures in Living Cells
Designed Ankyrin Repeat Proteins as Actin Labels of Distinct Cytoskeletal Structures in Living Cells Open
The orchestrated assembly of actin and actin-binding proteins into cytoskeletal structures coordinates cell morphology changes during migration, cytokinesis, and adaptation to external stimuli. The accurate and unbiased visualization of th…
View article: Heterodimerization of Endolysin Isoforms During Bacterial Infection by Staphylococcal Phage φ2638A
Heterodimerization of Endolysin Isoforms During Bacterial Infection by Staphylococcal Phage φ2638A Open
A bstract Bacteriophage endolysins targeting Gram-positive bacteria typically feature a modular architecture of one or more enzymatically active domains (EADs) and cell wall binding domains (CBDs). Several endolysins also feature internal …
View article: Development of LRRK2 designed ankyrin-repeat proteins
Development of LRRK2 designed ankyrin-repeat proteins Open
Leucine rich repeat kinase 2 (LRRK2) is a large multidomain protein containing two catalytic domains, a kinase and a GTPase, as well protein interactions domains, including a WD40 domain. The association of increased LRRK2 kinase activity …
View article: Assessing immunogenicity barriers of the HIV-1 envelope trimer
Assessing immunogenicity barriers of the HIV-1 envelope trimer Open
Understanding the balance between epitope shielding and accessibility on HIV-1 envelope (Env) trimers is essential to guide immunogen selection for broadly neutralizing antibody (bnAb) based vaccines. To investigate the antigenic space of …
View article: Nuclear myosin VI maintains replication fork stability
Nuclear myosin VI maintains replication fork stability Open
The actin cytoskeleton is of fundamental importance for cellular structure and plasticity. However, abundance and function of filamentous actin in the nucleus are still controversial. Here we show that the actin-based molecular motor myosi…
View article: Designed Ankyrin Repeat Proteins provide insights into the structure and function of CagI and are potent inhibitors of CagA translocation by the Helicobacter pylori type IV secretion system
Designed Ankyrin Repeat Proteins provide insights into the structure and function of CagI and are potent inhibitors of CagA translocation by the Helicobacter pylori type IV secretion system Open
The bacterial human pathogen Helicobacter pylori produces a type IV secretion system ( cag T4SS) to inject the oncoprotein CagA into gastric cells. The cag T4SS external pilus mediates attachment of the apparatus to the target cell and the…
View article: Supplementary Materials, Table 1-6, Figures 1-10 from Efficient Tumor Targeting with High-Affinity Designed Ankyrin Repeat Proteins: Effects of Affinity and Molecular Size
Supplementary Materials, Table 1-6, Figures 1-10 from Efficient Tumor Targeting with High-Affinity Designed Ankyrin Repeat Proteins: Effects of Affinity and Molecular Size Open
Supplementary Materials, Table 1-6, Figures 1-10 from Efficient Tumor Targeting with High-Affinity Designed Ankyrin Repeat Proteins: Effects of Affinity and Molecular Size
View article: Data from Efficient Tumor Targeting with High-Affinity Designed Ankyrin Repeat Proteins: Effects of Affinity and Molecular Size
Data from Efficient Tumor Targeting with High-Affinity Designed Ankyrin Repeat Proteins: Effects of Affinity and Molecular Size Open
Slow-clearing, tumor-targeting proteins such as monoclonal antibodies typically exhibit high tumor accumulation but low tissue contrast, whereas intermediate-sized proteins such as scFvs show faster clearance but only moderate tumor accumu…
View article: Supplementary Materials, Table 1-6, Figures 1-10 from Efficient Tumor Targeting with High-Affinity Designed Ankyrin Repeat Proteins: Effects of Affinity and Molecular Size
Supplementary Materials, Table 1-6, Figures 1-10 from Efficient Tumor Targeting with High-Affinity Designed Ankyrin Repeat Proteins: Effects of Affinity and Molecular Size Open
Supplementary Materials, Table 1-6, Figures 1-10 from Efficient Tumor Targeting with High-Affinity Designed Ankyrin Repeat Proteins: Effects of Affinity and Molecular Size
View article: Data from Efficient Tumor Targeting with High-Affinity Designed Ankyrin Repeat Proteins: Effects of Affinity and Molecular Size
Data from Efficient Tumor Targeting with High-Affinity Designed Ankyrin Repeat Proteins: Effects of Affinity and Molecular Size Open
Slow-clearing, tumor-targeting proteins such as monoclonal antibodies typically exhibit high tumor accumulation but low tissue contrast, whereas intermediate-sized proteins such as scFvs show faster clearance but only moderate tumor accumu…
View article: Designed Ankyrin Repeat Proteins for detecting prostate-specific antigen expression<i>in vivo</i>
Designed Ankyrin Repeat Proteins for detecting prostate-specific antigen expression<i>in vivo</i> Open
Late-stage prostate cancer often acquires resistance to conventional chemotherapies and transforms into a hormone-refractory, drug-resistant, and non-curative disease. Developing non-invasive tools to detect the biochemical changes that co…
View article: Designed ankyrin repeat proteins for detecting prostate-specific antigen expression <i>in vivo</i>
Designed ankyrin repeat proteins for detecting prostate-specific antigen expression <i>in vivo</i> Open
Prostate specific antigen binding Designed Ankyrin Repeat Proteins (DARPins) were selected and C-terminally conjugated for Gallium-68 radiolabelling. Two of the developed tracers showed specific uptake in mouse prostate tumour models.
View article: Designed Ankyrin Repeat Proteins provide insights into the structure and function of CagI and are potent inhibitors of CagA translocation by the<i>Helicobacter pylori</i>type IV secretion system
Designed Ankyrin Repeat Proteins provide insights into the structure and function of CagI and are potent inhibitors of CagA translocation by the<i>Helicobacter pylori</i>type IV secretion system Open
The bacterial human pathogen Helicobacter pylori produces a type IV secretion system ( cag T4SS) to inject the oncoprotein CagA into gastric cells. The cag T4SS external pilus mediates attachment of the apparatus to the target cell and the…
View article: A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology
A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology Open
Neuroscience currently requires the use of antibodies to study synaptic proteins, where antibody binding is used as a correlate to define the presence, plasticity, and regulation of synapses. Gephyrin is an inhibitory synaptic scaffolding …
View article: Author response: A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology
Author response: A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Neuroscience currently…
View article: Nuclear myosin VI maintains replication fork stability
Nuclear myosin VI maintains replication fork stability Open
The actin cytoskeleton is of fundamental importance for cellular structure and plasticity. However, abundance and function of filamentous (F-) actin in the nucleus are still controversial. Here we show that the actin-based molecular motor …
View article: A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology
A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology Open
Neuroscience currently requires the use of antibodies to study synaptic proteins, where antibody binding is used as a correlate to define the presence, plasticity, and regulation of synapses. Gephyrin is an inhibitory synaptic scaffolding …
View article: Disrupting the HDAC6-ubiquitin interaction impairs infection by influenza and Zika virus and cellular stress pathways
Disrupting the HDAC6-ubiquitin interaction impairs infection by influenza and Zika virus and cellular stress pathways Open
The deacetylase HDAC6 has tandem catalytic domains and a zinc finger domain (ZnF) binding ubiquitin (Ub). While the catalytic domain has an antiviral effect, the ZnF facilitates influenza A virus (IAV) infection and cellular stress respons…
View article: The RGD-binding integrins αvβ6 and αvβ8 are receptors for mouse adenovirus-1 and -3 infection
The RGD-binding integrins αvβ6 and αvβ8 are receptors for mouse adenovirus-1 and -3 infection Open
Mammalian adenoviruses (AdVs) comprise more than ~350 types including over 100 human (HAdVs) and just three mouse AdVs (MAdVs). While most HAdVs initiate infection by high affinity/avidity binding of their fiber knob (FK) protein to either…