Birgit Tiemann
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View article: <i>N</i>‐Glycoproteomics of the Apicomplexan Parasite <i>Toxoplasma gondii</i>
<i>N</i>‐Glycoproteomics of the Apicomplexan Parasite <i>Toxoplasma gondii</i> Open
Protein N ‐glycosylation influences protein folding, stability, and trafficking, and has prominent functions in cell–cell adhesion and recognition. For the parasite Toxoplasma gondii , N ‐glycosylation of proteins is crucial for initial ad…
View article: Conserved cysteines prevent <i>C</i> ‐mannosylation of mucin Cys domains
Conserved cysteines prevent <i>C</i> ‐mannosylation of mucin Cys domains Open
Mucins are major components of the mucus. Besides the highly O ‐glycosylated tandem repeat domains, mucins contain Cys domains (CysDs). CysDs contain conserved disulfide‐forming cysteine residues as well as a WxxW motif. Since this is the …
View article: A Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C- and O-Mannosylated Peptides
A Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C- and O-Mannosylated Peptides Open
Mass spectrometry (MS) easily detects C-mannosylated peptides from purified proteins but not from complex biological samples. Enrichment of specific glycopeptides by lectin affinity prior to MS analysis has been widely applied to support g…
View article: NMR Spectroscopic Characterization of the C‐Mannose Conformation in a Thrombospondin Repeat Using a Selective Labeling Approach
NMR Spectroscopic Characterization of the C‐Mannose Conformation in a Thrombospondin Repeat Using a Selective Labeling Approach Open
Despite the great interest in glycoproteins, structural information reporting on conformation and dynamics of the sugar moieties are limited. We present a new biochemical method to express proteins with glycans that are selectively labeled…
View article: NMR Spectroscopic Characterization of the C‐Mannose Conformation in a Thrombospondin Repeat Using a Selective Labeling Approach
NMR Spectroscopic Characterization of the C‐Mannose Conformation in a Thrombospondin Repeat Using a Selective Labeling Approach Open
Despite the great interest in glycoproteins, structural information reporting on conformation and dynamics of the sugar moieties are limited. We present a new biochemical method to express proteins with glycans that are selectively labeled…
View article: C-mannosylation supports folding and enhances stability of thrombospondin repeats
C-mannosylation supports folding and enhances stability of thrombospondin repeats Open
Previous studies demonstrated importance of C-mannosylation for efficient protein secretion. To study its impact on protein folding and stability, we analyzed both C-mannosylated and non-C-mannosylated thrombospondin type 1 repeats (TSRs) …
View article: Author response: C-mannosylation supports folding and enhances stability of thrombospondin repeats
Author response: C-mannosylation supports folding and enhances stability of thrombospondin repeats Open
View article: Distinct <i>C-</i> mannosylation of netrin receptor thrombospondin type 1 repeats by mammalian DPY19L1 and DPY19L3
Distinct <i>C-</i> mannosylation of netrin receptor thrombospondin type 1 repeats by mammalian DPY19L1 and DPY19L3 Open
Significance Protein C -mannosylation is restricted to animals and some related species. The C -mannosyltransferases probably evolved from enzymes responsible for N -glycosylation in the endoplasmic reticulum and are therefore expected to …