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View article: Enrichment and characterization of steroid-degrading microbes for targeted removal of steroid hormone micropollutants in small-scale wastewater treatment solutions
Enrichment and characterization of steroid-degrading microbes for targeted removal of steroid hormone micropollutants in small-scale wastewater treatment solutions Open
In remote areas, wastewater treatment typically occurs directly at the source in decentralized systems, which often work well in removing biochemical oxygen demand, nitrogen, and phosphorus, but not micropollutants like pharmaceuticals and…
View article: Chicken Manure as a Sustainable Bile Acid Source for Biotechnological Applications
Chicken Manure as a Sustainable Bile Acid Source for Biotechnological Applications Open
Ursodeoxycholic acid (UDCA) is widely administered to dissolve gallstones, treat liver disorders and reduce blood cholesterol levels. This study investigated fresh and dried chicken manure as a sustainable bioresource for chenodeoxycholic …
View article: 7β-Hydroxysteroid dehydratase Hsh3 eliminates the 7-hydroxy group of the bile salt ursodeoxycholate during degradation by <i>Sphingobium</i> sp. strain Chol11 and other <i>Sphingomonadaceae</i>
7β-Hydroxysteroid dehydratase Hsh3 eliminates the 7-hydroxy group of the bile salt ursodeoxycholate during degradation by <i>Sphingobium</i> sp. strain Chol11 and other <i>Sphingomonadaceae</i> Open
Bile salts are steroids with distinctive hydroxylation patterns that are produced and excreted by vertebrates. In contrast to common human bile salts, ursodeoxycholate (UDCA) has a 7-hydroxy group in β-configuration and is used in large am…
View article: 7β-hydroxysteroid dehydratase Hsh3 eliminates the 7-hydroxy group of the bile salt ursodeoxycholate during degradation by<i>Sphingobium</i>sp. strain Chol11 and other<i>Sphingomonadaceae</i>
7β-hydroxysteroid dehydratase Hsh3 eliminates the 7-hydroxy group of the bile salt ursodeoxycholate during degradation by<i>Sphingobium</i>sp. strain Chol11 and other<i>Sphingomonadaceae</i> Open
Bile salts are steroids with distinctive hydroxylation patterns that are produced and excreted by vertebrates. In contrast to common human bile salts, ursodeoxycholate (UDCA) has a 7-hydroxy group in β-configuration and is used in large am…
View article: Field Investigations and Service Life Assessment of Geosynthetic Filters in Tidally Influenced Revetments in Cases of Ochre Clogging
Field Investigations and Service Life Assessment of Geosynthetic Filters in Tidally Influenced Revetments in Cases of Ochre Clogging Open
In recent decades, there have been individual cases of damage to the revetments of the German North Sea estuaries due to clogging by precipitated ochre products. This process is defined as ochre clogging and has been extensively explained …
View article: Bacteria use a catabolic patchwork pathway of apparently recent origin for degradation of the synthetic buffer compound TRIS
Bacteria use a catabolic patchwork pathway of apparently recent origin for degradation of the synthetic buffer compound TRIS Open
The synthetic buffer compound TRIS (2-amino-2-(hydroxymethyl)propane-1,3-diol) is used in countless applications, and no detailed information on its degradation has been published so far. Herein, we describe the discovery of a complete bac…
View article: A domesticated photoautotrophic microbial community as a biofilm model system for analyzing the influence of plastic surfaces on invertebrate grazers in limnic environments
A domesticated photoautotrophic microbial community as a biofilm model system for analyzing the influence of plastic surfaces on invertebrate grazers in limnic environments Open
The environmental fate of plastic particles in water bodies is influenced by microbial biofilm formation. Invertebrate grazers may be affected when foraging biofilms on plastics compared to biofilms on natural substrata but the mechanistic…
View article: A patchwork pathway of apparently recent origin enables degradation of the synthetic buffer compound TRIS in bacteria
A patchwork pathway of apparently recent origin enables degradation of the synthetic buffer compound TRIS in bacteria Open
The widely used synthetic buffer compound TRIS was long considered to be biologically inert. Herein, we describe the discovery of a complete bacterial degradation pathway for TRIS. By serendipity, a Pseudomonas strain was isolated from sew…
View article: Biogas digestate as a sustainable phytosterol source for biotechnological cascade valorization
Biogas digestate as a sustainable phytosterol source for biotechnological cascade valorization Open
Every year, several million tonnes of anaerobic digestate are produced worldwide as a by‐product of the biogas industry, most of which is applied as agricultural fertilizer. However, in the context of a circular bioeconomy, more sustainabl…
View article: Investigations on the Degradation of the Bile Salt Cholate via the 9,10-Seco-Pathway Reveals the Formation of a Novel Recalcitrant Steroid Compound by a Side Reaction in Sphingobium sp. Strain Chol11
Investigations on the Degradation of the Bile Salt Cholate via the 9,10-Seco-Pathway Reveals the Formation of a Novel Recalcitrant Steroid Compound by a Side Reaction in Sphingobium sp. Strain Chol11 Open
Bile salts such as cholate are steroid compounds from the digestive tracts of vertebrates, which enter the environment upon excretion, e.g., in manure. Environmental bacteria degrade bile salts aerobically via two pathway variants involvin…
View article: Comparative Analysis of Bile-Salt Degradation in <i>Sphingobium</i> sp. Strain Chol11 and Pseudomonas stutzeri Strain Chol1 Reveals Functional Diversity of Proteobacterial Steroid Degradation Enzymes and Suggests a Novel Pathway for Side Chain Degradation
Comparative Analysis of Bile-Salt Degradation in <i>Sphingobium</i> sp. Strain Chol11 and Pseudomonas stutzeri Strain Chol1 Reveals Functional Diversity of Proteobacterial Steroid Degradation Enzymes and Suggests a Novel Pathway for Side Chain Degradation Open
This study highlights the biochemical diversity of bacterial degradation of steroid compounds in different aspects. First, it further elucidates an unexplored variant in the degradation of bile-salt side chains by sphingomonads, a group of…
View article: Degradation of Bile Acids by Soil and Water Bacteria
Degradation of Bile Acids by Soil and Water Bacteria Open
Bile acids are surface-active steroid compounds with a C5 carboxylic side chain at the steroid nucleus. They are produced by vertebrates, mainly functioning as emulsifiers for lipophilic nutrients, as signaling compounds, and as an antimic…
View article: Proteome, Bioinformatic, and Functional Analyses Reveal a Distinct and Conserved Metabolic Pathway for Bile Salt Degradation in the <i>Sphingomonadaceae</i>
Proteome, Bioinformatic, and Functional Analyses Reveal a Distinct and Conserved Metabolic Pathway for Bile Salt Degradation in the <i>Sphingomonadaceae</i> Open
This study highlights the biochemical diversity of bacterial degradation of steroid compounds in particular bile salts. Furthermore, it substantiates and advances knowledge of a variant pathway for degradation of steroids by sphingomonads,…
View article: Comparative analysis of bile-salt degradation in <i>Sphingobium</i> sp. strain Chol11 and <i>P. stutzeri</i> Chol1 reveals functional diversity of β-proteobacterial steroid degradation enzymes and suggests a novel reaction sequence for side-chain degradation involving a hydroxylation step
Comparative analysis of bile-salt degradation in <i>Sphingobium</i> sp. strain Chol11 and <i>P. stutzeri</i> Chol1 reveals functional diversity of β-proteobacterial steroid degradation enzymes and suggests a novel reaction sequence for side-chain degradation involving a hydroxylation step Open
The reaction sequence for aerobic degradation of bile salts by environmental bacteria resembles degradation of other steroid compounds. Recent findings show that bacteria belonging to the Sphingomonadaceae use a pathway variant for bile-sa…
View article: A Complex of LaoA and LaoB Acts as a Tat-Dependent Dehydrogenase for Long-Chain Alcohols in Pseudomonas aeruginosa
A Complex of LaoA and LaoB Acts as a Tat-Dependent Dehydrogenase for Long-Chain Alcohols in Pseudomonas aeruginosa Open
Pseudomonas aeruginosa is a major threat to public health. Its ability to thrive in clinical settings, water distribution systems, or even jet fuel tanks is linked to detoxification and degradation of diverse hydrophobic substrates that ar…
View article: Proteome, bioinformatic and functional analyses reveal a distinct and conserved metabolic pathway for bile salt degradation in the <i>Sphingomonadaceae</i>
Proteome, bioinformatic and functional analyses reveal a distinct and conserved metabolic pathway for bile salt degradation in the <i>Sphingomonadaceae</i> Open
Bile salts are amphiphilic steroids with a C 5 carboxylic side chain with digestive functions in vertebrates. Upon excretion, they are degraded by environmental bacteria. Degradation of the bile-salt steroid skeleton resembles the well-stu…
View article: Substrate Inhibition of 5β-Δ4-3-Ketosteroid Dehydrogenase in Sphingobium sp. Strain Chol11 Acts as Circuit Breaker During Growth With Toxic Bile Salts
Substrate Inhibition of 5β-Δ4-3-Ketosteroid Dehydrogenase in Sphingobium sp. Strain Chol11 Acts as Circuit Breaker During Growth With Toxic Bile Salts Open
In contrast to many steroid hormones and cholesterol, mammalian bile salts are 5β-steroids, which leads to a bent structure of the steroid core. Bile salts are surface-active steroids excreted into the environment in large amounts, where t…
View article: A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to l-lysine
A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to l-lysine Open
Chitin is an abundant waste product from shrimp and mushroom industries and as such, an appropriate secondary feedstock for biotechnological processes. However, chitin is a crystalline substrate embedded in complex biological matrices, and…
View article: Evidence of Interdomain Ammonium Cross-Feeding From Methylamine- and Glycine Betaine-Degrading Rhodobacteraceae to Diatoms as a Widespread Interaction in the Marine Phycosphere
Evidence of Interdomain Ammonium Cross-Feeding From Methylamine- and Glycine Betaine-Degrading Rhodobacteraceae to Diatoms as a Widespread Interaction in the Marine Phycosphere Open
Dissolved organic nitrogen (DON) compounds such as methylamines (MAs) and glycine betaine (GBT) occur at detectable concentrations in marine habitats and are also produced and released by microalgae. For many marine bacteria, these DON com…
View article: Screening of Bacterial Quorum Sensing Inhibitors in a Vibrio fischeri LuxR-Based Synthetic Fluorescent E. coli Biosensor
Screening of Bacterial Quorum Sensing Inhibitors in a Vibrio fischeri LuxR-Based Synthetic Fluorescent E. coli Biosensor Open
A library of 23 pure compounds of varying structural and chemical characteristics was screened for their quorum sensing (QS) inhibition activity using a synthetic fluorescent Escherichia coli biosensor that incorporates a modified version …
View article: Genetic and physiological analysis of biofilm formation on different plastic surfaces by Sphingomonas sp. strain S2M10 reveals an essential function of sphingan biosynthesis
Genetic and physiological analysis of biofilm formation on different plastic surfaces by Sphingomonas sp. strain S2M10 reveals an essential function of sphingan biosynthesis Open
Alphaproteobacteria belonging to the group of the sphingomonads are frequently found in biofilms colonizing pure-water systems, where they cause technical and hygienic problems. In this study, physiological properties of sphingomonads for …
View article: Sulfate Ester Detergent Degradation in<i>Pseudomonas aeruginosa</i>Is Subject to both Positive and Negative Regulation
Sulfate Ester Detergent Degradation in<i>Pseudomonas aeruginosa</i>Is Subject to both Positive and Negative Regulation Open
Bacterial degradation of toxic compounds is important not only for bioremediation but also for the colonization of hostile anthropogenic environments in which biocides are being used. This study with Pseudomonas aeruginosa expands our know…
View article: Identification of a novel chitinase from<i>Aeromonas hydrophila AH-1N</i>for the degradation of chitin within fungal mycelium
Identification of a novel chitinase from<i>Aeromonas hydrophila AH-1N</i>for the degradation of chitin within fungal mycelium Open
Defined organic waste products are ideal and sustainable secondary feedstocks for production organisms in microbial biotechnology. Chitin from mycelia of fungal fermentation processes represents a homogeneous and constantly available waste…
View article: SpoT-Mediated Regulation and Amino Acid Prototrophy Are Essential for Pyocyanin Production During Parasitic Growth of Pseudomonas aeruginosa in a Co-culture Model System With Aeromonas hydrophila
SpoT-Mediated Regulation and Amino Acid Prototrophy Are Essential for Pyocyanin Production During Parasitic Growth of Pseudomonas aeruginosa in a Co-culture Model System With Aeromonas hydrophila Open
The opportunistic pathogen Pseudomonas aeruginosa employs its complex quorum sensing (QS) network to regulate the expression of virulence factors such as pyocyanin. Besides cell density, QS in this bacterium is co-regulated by environmenta…
View article: LaoABCR, a Novel System for Oxidation of Long-Chain Alcohols Derived from SDS and Alkane Degradation in Pseudomonas aeruginosa
LaoABCR, a Novel System for Oxidation of Long-Chain Alcohols Derived from SDS and Alkane Degradation in Pseudomonas aeruginosa Open
The opportunistic pathogen Pseudomonas aeruginosa strain PAO1 is able to use a variety of organic pollutants as growth substrates, including the anionic detergent sodium dodecyl sulfate (SDS) and long-chain alkanes. While the enzymes initi…
View article: Functional Characterization of Three Specific Acyl-Coenzyme A Synthetases Involved in Anaerobic Cholesterol Degradation in Sterolibacterium denitrificans Chol1S
Functional Characterization of Three Specific Acyl-Coenzyme A Synthetases Involved in Anaerobic Cholesterol Degradation in Sterolibacterium denitrificans Chol1S Open
The denitrifying betaproteobacterium Sterolibacterium denitrificans Chol1S catabolizes steroids such as cholesterol via an oxygen-independent pathway. It involves enzyme reaction sequences described for aerobic cholesterol and bile acid de…
View article: Genome Sequence of the Bile Salt-Degrading Bacterium <i>Novosphingobium</i> sp. Strain Chol11, a Model Organism for Bacterial Steroid Catabolism
Genome Sequence of the Bile Salt-Degrading Bacterium <i>Novosphingobium</i> sp. Strain Chol11, a Model Organism for Bacterial Steroid Catabolism Open
Many bacteria from different phylogenetic groups are able to degrade eukaryotic steroid compounds, but the underlying metabolic pathways are still not well understood. Novosphingobium sp. strain Chol11 is a steroid-degrading alphaproteobac…
View article: A Novel Steroid-Coenzyme A Ligase from Novosphingobium sp. Strain Chol11 Is Essential for an Alternative Degradation Pathway for Bile Salts
A Novel Steroid-Coenzyme A Ligase from Novosphingobium sp. Strain Chol11 Is Essential for an Alternative Degradation Pathway for Bile Salts Open
Bile salts such as cholate are steroid compounds with a C 5 carboxylic side chain and occur ubiquitously in vertebrates. Upon their excretion into soils and waters, bile salts can serve as growth substrates for diverse bacteria. Novosphing…