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View article: Increased thermal stability of FGF10 leads to ectopic signaling during development
Increased thermal stability of FGF10 leads to ectopic signaling during development Open
Fibroblast growth factors (FGFs) control organ morphogenesis during development as well as tissue homeostasis and repair in the adult organism. Despite their importance, many mechanisms that regulate FGF function are still poorly understoo…
View article: FGFR2 residence in primary cilia is necessary for epithelial cell signaling
FGFR2 residence in primary cilia is necessary for epithelial cell signaling Open
Primary cilium projects from cells to provide a communication platform with neighboring cells and the surrounding environment. This is ensured by the selective entry of membrane receptors and signaling molecules, producing fine-tuned and e…
View article: Achondroplasia: aligning mouse model with human clinical studies shows crucial importance of immediate postnatal start of the therapy
Achondroplasia: aligning mouse model with human clinical studies shows crucial importance of immediate postnatal start of the therapy Open
Achondroplasia is the most common form of human dwarfism caused by mutations in the FGFR3 receptor tyrosine kinase. Current therapy begins at 2 years of age and improves longitudinal growth but does not address the cranial malformations in…
View article: Discovery of Two Highly Selective Structurally Orthogonal Chemical Probes for Activin Receptor-like Kinases 1 and 2
Discovery of Two Highly Selective Structurally Orthogonal Chemical Probes for Activin Receptor-like Kinases 1 and 2 Open
Activin receptor-like kinases 1-7 (ALK1-7) regulate a complex network of SMAD-independent as well as SMAD-dependent signaling pathways. One of the widely used inhibitors for functional investigations of these processes, in particular for b…
View article: Ligand bias underlies differential signaling of multiple FGFs via FGFR1
Ligand bias underlies differential signaling of multiple FGFs via FGFR1 Open
The differential signaling of multiple FGF ligands through a single fibroblast growth factor (FGF) receptor (FGFR) plays an important role in embryonic development. Here, we use quantitative biophysical tools to uncover the mechanism behin…
View article: Author Response: Ligand bias underlies differential signaling of multiple FGFs via FGFR1
Author Response: Ligand bias underlies differential signaling of multiple FGFs via FGFR1 Open
Full text Figures and data Peer review Side by side Abstract eLife assessment Introduction Results Discussion Materials and methods Data availability References Article and author information Abstract The differential signaling of multiple…
View article: Inducible protein expression in stably transfected cells paves the way toward in-cell NMR studies in defined physiological states and 3D tissue cultures.
Inducible protein expression in stably transfected cells paves the way toward in-cell NMR studies in defined physiological states and 3D tissue cultures. Open
In-cell NMR spectroscopy is the sole technique for characterizing protein structure, dynamics, and interactions in living human cells at atomic resolution. However, its applications have been restricted to asynchronous single-cell suspensi…
View article: Inducible protein expression in stably transfected cells paves the way toward in-cell NMR studies in defined physiological states and 3D tissue cultures.
Inducible protein expression in stably transfected cells paves the way toward in-cell NMR studies in defined physiological states and 3D tissue cultures. Open
In-cell NMR spectroscopy is the sole technique for characterizing protein structure, dynamics, and interactions in living human cells at atomic resolution. However, its applications have been restricted to asynchronous single-cell suspensi…
View article: Author Response: Ligand bias underlies differential signaling of multiple FGFs via FGFR1
Author Response: Ligand bias underlies differential signaling of multiple FGFs via FGFR1 Open
The mechanism of differential signaling of multiple FGF ligands through a single FGF receptor is poorly understood. Here, we use biophysical tools to quantify multiple aspects of FGFR1 signaling in response to FGF4, FGF8 and FGF9: potency,…
View article: Ligand bias underlies differential signaling of multiple FGFs via FGFR1
Ligand bias underlies differential signaling of multiple FGFs via FGFR1 Open
The mechanism of differential signaling of multiple FGF ligands through a single FGF receptor is poorly understood. Here, we use biophysical tools to quantify multiple aspects of FGFR1 signaling in response to FGF4, FGF8 and FGF9: potency,…
View article: Joint Public Review: Ligand bias underlies differential signaling of multiple FGFs via FGFR1
Joint Public Review: Ligand bias underlies differential signaling of multiple FGFs via FGFR1 Open
The mechanism of differential signaling of multiple FGF ligands through a single FGF receptor is poorly understood. Here, we use biophysical tools to quantify multiple aspects of FGFR1 signaling in response to FGF4, FGF8 and FGF9: potency,…
View article: Author Response: Ligand bias underlies differential signaling of multiple FGFs via FGFR1
Author Response: Ligand bias underlies differential signaling of multiple FGFs via FGFR1 Open
The mechanism of differential signaling of multiple FGF ligands through a single FGF receptor is poorly understood. Here, we use biophysical tools to quantify multiple aspects of FGFR1 signaling in response to FGF4, FGF8 and FGF9: potency,…
View article: Ligand bias underlies differential signaling of multiple FGFs via FGFR1
Ligand bias underlies differential signaling of multiple FGFs via FGFR1 Open
FGFR1 signals differently in response to the fgf ligands FGF4, FGF8 and FGF9, but the mechanism behind the differential ligand recognition is poorly understood. Here, we use biophysical tools to quantify multiple aspects of FGFR1 signaling…
View article: Bias in FGFR1 signaling in response to FGF4, FGF8, and FGF9
Bias in FGFR1 signaling in response to FGF4, FGF8, and FGF9 Open
FGFR1 signals differently in response to the fgf ligands FGF4, FGF8 and FGF9, but the mechanism behind the differential ligand recognition is poorly understood. Here, we use biophysical tools to quantify multiple aspects of FGFR1 signaling…
View article: Ligand bias underlies differential signaling of multiple FGFs via FGFR1
Ligand bias underlies differential signaling of multiple FGFs via FGFR1 Open
The differential signaling of multiple FGF ligands through a single fibroblast growth factor (FGF) receptor (FGFR) plays an important role in embryonic development. Here, we use quantitative biophysical tools to uncover the mechanism behin…
View article: Supplementary Figures S1-3, Tables S1-3 from HIC1 Tumor Suppressor Loss Potentiates TLR2/NF-κB Signaling and Promotes Tissue Damage–Associated Tumorigenesis
Supplementary Figures S1-3, Tables S1-3 from HIC1 Tumor Suppressor Loss Potentiates TLR2/NF-κB Signaling and Promotes Tissue Damage–Associated Tumorigenesis Open
Supplementary Figures S1-3, Tables S1-3. Supplementary Figure S1. Heatmaps depicting gene expression in Hic1flox/flox MEFs treated with 4- OHT when compared to MEFs treated with vehicle (ethanol) only. Genes (299 in total) displaying signi…
View article: Supplementary Figures S1-3, Tables S1-3 from HIC1 Tumor Suppressor Loss Potentiates TLR2/NF-κB Signaling and Promotes Tissue Damage–Associated Tumorigenesis
Supplementary Figures S1-3, Tables S1-3 from HIC1 Tumor Suppressor Loss Potentiates TLR2/NF-κB Signaling and Promotes Tissue Damage–Associated Tumorigenesis Open
Supplementary Figures S1-3, Tables S1-3. Supplementary Figure S1. Heatmaps depicting gene expression in Hic1flox/flox MEFs treated with 4- OHT when compared to MEFs treated with vehicle (ethanol) only. Genes (299 in total) displaying signi…
View article: Data from HIC1 Tumor Suppressor Loss Potentiates TLR2/NF-κB Signaling and Promotes Tissue Damage–Associated Tumorigenesis
Data from HIC1 Tumor Suppressor Loss Potentiates TLR2/NF-κB Signaling and Promotes Tissue Damage–Associated Tumorigenesis Open
Hypermethylated in cancer 1 (HIC1) represents a prototypic tumor suppressor gene frequently inactivated by DNA methylation in many types of solid tumors. The gene encodes a sequence-specific transcriptional repressor controlling expression…
View article: Data from HIC1 Tumor Suppressor Loss Potentiates TLR2/NF-κB Signaling and Promotes Tissue Damage–Associated Tumorigenesis
Data from HIC1 Tumor Suppressor Loss Potentiates TLR2/NF-κB Signaling and Promotes Tissue Damage–Associated Tumorigenesis Open
Hypermethylated in cancer 1 (HIC1) represents a prototypic tumor suppressor gene frequently inactivated by DNA methylation in many types of solid tumors. The gene encodes a sequence-specific transcriptional repressor controlling expression…
View article: Analysis of domain-specific function reveals significant plasticity in BCR-ABL signaling
Analysis of domain-specific function reveals significant plasticity in BCR-ABL signaling Open
Discontinuation of the tyrosine kinase inhibitor (TKI) therapy leads to relapse in chronic myeloid leukemia (CML), suggesting that TKIs do not completely eliminate cancer cells. Recently, we showed that TKIs inhibit catalytic activity of B…
View article: LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters
LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters Open
Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional lu…
View article: LuminoCell: a versatile and affordable luminometer platform for monitoring in-cell luciferase-based reporters
LuminoCell: a versatile and affordable luminometer platform for monitoring in-cell luciferase-based reporters Open
Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional lu…
View article: Ligand bias underlies differential signaling of multiple FGFs via FGFR1
Ligand bias underlies differential signaling of multiple FGFs via FGFR1 Open
The mechanism of differential signaling of multiple FGF ligands through a single FGF receptor is poorly understood. Here, we use biophysical tools to quantify multiple aspects of FGFR1 signaling in response to FGF4, FGF8 and FGF9: potency,…
View article: 4-PBA Treatment Improves Bone Phenotypes in the Aga2 Mouse Model of Osteogenesis Imperfecta
4-PBA Treatment Improves Bone Phenotypes in the Aga2 Mouse Model of Osteogenesis Imperfecta Open
Osteogenesis imperfecta (OI) is a genetically heterogenous disorder most often due to heterozygosity for mutations in the type I procollagen genes, COL1A1 or COL1A2. The disorder is characterized by bone fragility leading to increased frac…