Boris Greber
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View article: Sequential factor delivery enables efficient workflow for universal gene editing in clinical grade iPS cells
Sequential factor delivery enables efficient workflow for universal gene editing in clinical grade iPS cells Open
Human induced pluripotent stem cells (iPSCs) are gaining momentum as a powerful starting material in cell therapy. To fully harness their potential, CRISPR technology permits endogenous gene modifications as well as the introduction of adv…
View article: CytoBead ANA 2 assay - a novel method for the detection of antinuclear antibodies
CytoBead ANA 2 assay - a novel method for the detection of antinuclear antibodies Open
Detection of anti-nuclear autoantibodies (ANA) is based on a two-step algorithm including indirect immunofluorescence (IIF) on HEp2 cells and subsequent reflex/confirmatory testing for specific autoantibodies. Simultaneous cell- and microb…
View article: CytoBead ANA 2 assay - a novel method for the detection of antinuclear antibodies
CytoBead ANA 2 assay - a novel method for the detection of antinuclear antibodies Open
Background: Detection of anti-nuclear autoantibodies (ANA) is based on a two-step algorithm including indirect immunofluorescence (IIF) on HEp2 cells and subsequent reflex/confirmatory testing for specific autoantibodies. Simultaneous cell…
View article: Abstract 12: Cord Blood Banking, Technical and Clinical grade GMP- Development of Advanced Therapy Products as HLA-Homozygous iPSC-derived Cardiomyocytes
Abstract 12: Cord Blood Banking, Technical and Clinical grade GMP- Development of Advanced Therapy Products as HLA-Homozygous iPSC-derived Cardiomyocytes Open
Introduction The Cord Blood Bank (CBB) in Düsseldorf stores 21477 active cryopreserved cord blood units (CBUs) licensed by the Paul-Ehrlich Institute, with 1517 transplants delivered worldwide. We have previously documented a stable storag…
View article: GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy
GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy Open
Cell therapeutic applications based on induced pluripotent stem cells (iPSCs) appear highly promising and challenging at the same time. Good manufacturing practice (GMP) regulations impose necessary yet demanding requirements for quality a…
View article: Abstract 23 HLA-Homozygous iPSC from Cord Blood for the Generation of Cardiomyocytes for Allogeneic Cell Therapy to the Heart
Abstract 23 HLA-Homozygous iPSC from Cord Blood for the Generation of Cardiomyocytes for Allogeneic Cell Therapy to the Heart Open
Introduction Based on a previous grant on HLA-homozygous (HLAh) iPS-GMP (and ATMP conform) generation from allogeneic licensed cord blood (CB) units, the Düsseldorf CBB could provide the most frequent HLA-types for reprogramming (Liedtke e…
View article: Pathophysiological Mechanisms of Cardiac Dysfunction in Transgenic Mice with Viral Myocarditis
Pathophysiological Mechanisms of Cardiac Dysfunction in Transgenic Mice with Viral Myocarditis Open
Viral myocarditis is pathologically associated with RNA viruses such as coxsackievirus B3 (CVB3), or more recently, with SARS-CoV-2, but despite intensive research, clinically proven treatment is limited. Here, by use of a transgenic mouse…
View article: The first versatile human iPSC-based model of ectopic virus induction allows new insights in RNA-virus disease
The first versatile human iPSC-based model of ectopic virus induction allows new insights in RNA-virus disease Open
A detailed description of pathophysiological effects that viruses exert on their host is still challenging. For the first time, we report a highly controllable viral expression model based on an iPS-cell line from a healthy human donor. Th…
View article: Effects of Ceramide Accumulation in Cardiomyocytes Derived from human-induced Pluripotent Stem Cells on Mitochondrial Function and Mitophagy
Effects of Ceramide Accumulation in Cardiomyocytes Derived from human-induced Pluripotent Stem Cells on Mitochondrial Function and Mitophagy Open
BackgroundOversupply of fatty acids (FAs) to cardiomyocytes (CMs) is associated with increased ceramide content and elevated the risk of lipotoxic cardiomyopathy. Here we investigate the role of ceramide accumulation on mitochondrial funct…
View article: Discovery of retinoic acid receptor agonists as proliferators of cardiac progenitor cells through a phenotypic screening approach
Discovery of retinoic acid receptor agonists as proliferators of cardiac progenitor cells through a phenotypic screening approach Open
Identification of small molecules with the potential to selectively proliferate cardiac progenitor cells (CPCs) will aid our understanding of the signaling pathways and mechanisms involved and could ultimately provide tools for regenerativ…
View article: The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation
The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation Open
BAF complexes are composed of different subunits with varying functional and developmental roles, although many subunits have not been examined in depth. Here we show that the Baf45 subunit Dpf2 maintains pluripotency and ESC differentiati…
View article: Electroconductive Biohybrid Hydrogel for Enhanced Maturation and Beating Properties of Engineered Cardiac Tissues
Electroconductive Biohybrid Hydrogel for Enhanced Maturation and Beating Properties of Engineered Cardiac Tissues Open
Cardiac tissue engineering is a promising strategy to treat heart failure. Yet, several issues remain to be resolved including the prevention of arrhythmia caused by inefficient electrical coupling within the graft and between graft and ho…
View article: Revised roles of ISL1 in a hES cell-based model of human heart chamber specification
Revised roles of ISL1 in a hES cell-based model of human heart chamber specification Open
The transcription factor ISL1 is thought to be key for conveying the multipotent and proliferative properties of cardiac precursor cells. Here, we investigate its function upon cardiac induction of human embryonic stem cells. We find that …
View article: Author response: Revised roles of ISL1 in a hES cell-based model of human heart chamber specification
Author response: Revised roles of ISL1 in a hES cell-based model of human heart chamber specification Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract The transcription factor ISL1 is thought t…
View article: Adrenergic Stress Protection of Human iPS Cell-Derived Cardiomyocytes by Fast Kv7.1 Recycling
Adrenergic Stress Protection of Human iPS Cell-Derived Cardiomyocytes by Fast Kv7.1 Recycling Open
The fight-or-flight response (FFR), a physiological acute stress reaction, involves positive chronotropic and inotropic effects on heart muscle cells mediated through β-adrenoceptor activation. Increased systolic calcium is required to ena…
View article: The Influence Of Different Pathogens On The Lysozyme Activity Into Tissues Of Rat Oral Cavity
The Influence Of Different Pathogens On The Lysozyme Activity Into Tissues Of Rat Oral Cavity Open
Levitsky A. P., Ostafiichuk M. A., Uspenskii O. E., Boris G. Z., Furdychko A. I., Ginzhul I. V., Vasiuk V. L., Stepan V. T., Iarynich M. F., Stupak E. P. The influence of different pathogens on the lysozyme activity into tissues of rat ora…
View article: Cardiac Subtype-Specific Modeling of Kv1.5 Ion Channel Deficiency Using Human Pluripotent Stem Cells
Cardiac Subtype-Specific Modeling of Kv1.5 Ion Channel Deficiency Using Human Pluripotent Stem Cells Open
The ultrarapid delayed rectifier K+ current (IKur), mediated by Kv1.5 channels, constitutes a key component of the atrial action potential. Functional mutations in the underlying KCNA5 gene have been shown to cause hereditary forms of atri…
View article: Switch From Fetal to Adult <i>SCN5A</i> Isoform in Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes Unmasks the Cellular Phenotype of a Conduction Disease–Causing Mutation
Switch From Fetal to Adult <i>SCN5A</i> Isoform in Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes Unmasks the Cellular Phenotype of a Conduction Disease–Causing Mutation Open
Background Human induced pluripotent stem cell–derived cardiomyocytes (hi PSC ‐ CM s) can recapitulate features of ion channel mutations causing inherited rhythm disease. However, the lack of maturity of these cells is considered a signifi…
View article: The therapeutic effect of antidisbiotic means on the mucous sheath of rats digestive tract, obtaining peroxidized sunflower oil
The therapeutic effect of antidisbiotic means on the mucous sheath of rats digestive tract, obtaining peroxidized sunflower oil Open
Levitsky A. P., Gozhenko A. I., Bocharov A. V., Boris G. Z., Pustovoit P. I., Varava G. N. The therapeutic effect of antidisbiotic means on the mucous sheath of rats digestive tract, obtaining peroxidized sunflower oil. Journal of Educatio…