Brian C. Wilson
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Photodynamic therapy dosimetry: current status and the emerging challenge of immune stimulation Open
This article is not a comprehensive review, but rather intended to recognize past advances and current limitations, and to stimulate discussion of future directions in PDT dosimetry. Inadequate dosimetry may be a potential impediment to PD…
Comparison of multispectral singlet oxygen luminescence dosimetry and singlet oxygen explicit dosimetry in artificial phantom Open
We demonstrate that singlet oxygen ( ) detected through MSOLD can reliably quantify ROS concentrations in BPD-mediated PDT with accuracy comparable to the SOED model, which requires separate measurements of light fluence rate, ph…
Is seeing believing?—signal differentiation in a preclinical transbronchial imaging study implementing a composite optical fiber bronchoscope to detect a folate receptor-targeted near-infrared fluorophore Open
In situations of very close proximity of the COF to the bronchial wall (~1 mm), we detected both excitation and emission signals. When the distance was increased slightly, only the fluorescence emission signal was detected. Although these …
Emerging uses of 5-aminolevulinic-acid-induced protoporphyrin IX in medicine: a review of multifaceted, ubiquitous, molecular diagnostic, therapeutic, and theranostic opportunities Open
The 5-ALA-PpIX system has broad potential partly because of the ubiquity of the heme synthesis across many cell/tissue types, combined with natural selectivity, unique pharmacokinetics, bright fluorescence, and sufficiently strong singlet …
Deep learning-enabled fluorescence imaging for oral cancer margin classification in preclinical models Open
A DL-enabled SFDI system trained with in silico images demonstrates promise in providing margin assessment of oral cancer tumors.
View article: Cutaneous photosensitivity of phototheranostic porphyrin–lipid nanoparticles
Cutaneous photosensitivity of phototheranostic porphyrin–lipid nanoparticles Open
Skin photosensitization is a common challenge following intravenous administration of many photodynamic therapy (PDT) drugs, typically lasting days, weeks, or months in laboratory animals and patients. Symptoms of photosensitivity manifest…
Atomically-precise Au22(Lys-Cys-Lys)16 nanoclusters for radiation sensitization Open
Radiotherapy is a leading method for cancer treatment, effectively eliminating cancer cells but often causing collateral damage to surrounding healthy tissue. Radiosensitizers aim to enhance the therapeutic effects of radiotherapy while mi…
Deep-learning enabled combined measurement of tumour cell density and tumour infiltrating lymphocyte density as a prognostic biomarker in colorectal cancer Open
Background Within the colorectal cancer (CRC) tumour microenvironment, tumour infiltrating lymphocytes (TILs) and tumour cell density (TCD) are recognised prognostic markers. Measurement of TILs and TCD using deep-learning (DL) on haematox…
Real‐Time, <span>AI</span>‐Guided Photodynamic Laparoscopy Enhances Detection in a Rabbit Model of Peritoneal Cancer Metastasis Open
Accurate diagnosis is essential for effective cancer treatment, particularly in peritoneal surface malignancies, where failure to detect metastatic lesions can mislead the treatment plan. This study assessed the diagnostic accuracy of stag…
Analysis of Singlet Oxygen Luminescence Generated By Protoporphyrin IX Open
The effectiveness of photodynamic therapy (PDT) for cancer treatment relies on the generation of cytotoxic singlet oxygen (1O2) in type II PDT. Hence, monitoring of 1O2 generation during PDT enables optimal treatment delivery to the tumor …
Correction of Multispectral Singlet Oxygen Luminescent Dosimetry (MSOLD) for Tissue Optical Properties in Photofrin-Mediated Photodynamic Therapy Open
The direct detection of singlet-state oxygen (1O2) constitutes the holy grail dosimetric method for type-II photodynamic therapy (PDT), a goal that can be quantified using multispectral singlet oxygen near-infrared luminescence dosimetry (…
Correction of Multispectral Singlet Oxygen Luminescent Dosimetry (MSOLD) for Tissue Optical Properties in Photofrin-Mediated Photodynamic Therapy Open
Direct detection of singlet-state oxygen (1O2) constitutes the holy grail dosimetric method for type-II photodynamic therapy (PDT), a goal that can be quantified using multispectral singlet oxygen near-infrared luminescence dosimetry (MSOL…
Deep learning–enabled fluorescence imaging for surgical guidance: in silico training for oral cancer depth quantification Open
A DL-enabled SFDI system trained with in silico CSH demonstrates promise in defining the deep margins of oral tumors.
View article: Toward noncontact macroscopic imaging of multiple cancers using multi‐spectral inelastic scattering detection
Toward noncontact macroscopic imaging of multiple cancers using multi‐spectral inelastic scattering detection Open
Here we introduce a Raman spectroscopy approach combining multi‐spectral imaging and a new fluorescence background subtraction technique to image individual Raman peaks in less than 5 seconds over a square field‐of‐view of 1‐centimeter sid…
View article: Crystal structures of DCAF1-PROTAC-WDR5 ternary complexes provide insight into DCAF1 substrate specificity
Crystal structures of DCAF1-PROTAC-WDR5 ternary complexes provide insight into DCAF1 substrate specificity Open
Proteolysis-targeting chimeras (PROTACs) have been explored for the degradation of drug targets, particularly undruggable proteins, for more than two decades. They have been employed by various groups successfully, however only a handful o…
New Approaches to Photodynamic Therapy of Melanomas Open
We present new approaches to treat both primary cutaneous and ocular melanoma, with potential to reduce metastatic spread In mouse models optical clearing combined with dual-photosensitizer PDT is used to treat primary cutaneous xenogafts.…
Multispectral singlet oxygen luminescent dosimetry (MSOLD) for Photofrin-mediated PDT Open
Multispectral singlet oxygen dosimetry (MSOLD) provides a mean for direct detection of singlet oxygen, responsible for tumor kill for type II PDT. An InGaAs spectrometer or an InGaAs detector coupled with to narrow beam filters (1200, 1240…
Correlation of in vitro cell viability and cumulative singlet oxygen luminescence from protoporphyrin IX in mitochondria and plasma membrane Open
Direct 1O2 luminescence dosimetry's potential value for comparing the PDT sensitivity of different subcellular organelles was demonstrated. This could be useful for developing subcellular targeted novel photosensitizers to enhance PDT effi…
Correlation of in vitro cell viability and cumulative singlet oxygen luminescence from protoporphyrin IX in mitochondria and plasma membrane Open
Photodynamic therapy (PDT) can be targeted toward different subcellular localizations and it is proposed that different subcellular targets vary in their sensitivity to photobiological damage. Since singlet oxygen (1O2) has a very short li…
Femtosecond pulsed laser photodynamic therapy activates melanin and eradicates malignant melanoma Open
Photodynamic therapy (PDT) relies on a series of photophysical and photochemical reactions leading to cell death. While effective for various cancers, PDT has been less successful in treating pigmented melanoma due to high light absorption…
Investigating the effects of a temperature dependent photodynamic dose: A numerical study Open
Characterizing synergistic effects of PDT and PTT could enhance treatment planning. Future work is ongoing to implement additional variables, such as photosensitizer photobleaching, and spatial and temporally varying oxygenation.