Brian G. Petrich
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View article: Directing the migration of serum-free, ex vivo-expanded Vγ9Vδ2 T cells
Directing the migration of serum-free, ex vivo-expanded Vγ9Vδ2 T cells Open
Vγ9Vδ2 T cells represent a promising cancer therapy platform because the implementation of allogenic, off-the-shelf product candidates is possible. However, intravenous administration of human Vγ9Vδ2 T cells manufactured under good manufac…
View article: Ligand-based targeting of c-kit using engineered γδ T cells as a strategy for treating acute myeloid leukemia
Ligand-based targeting of c-kit using engineered γδ T cells as a strategy for treating acute myeloid leukemia Open
The application of immunotherapies such as chimeric antigen receptor (CAR) T therapy or bi-specific T cell engager (BiTE) therapy to manage myeloid malignancies has proven more challenging than for B-cell malignancies. This is attributed t…
View article: Molecular mechanocytometry using tension-activated cell tagging
Molecular mechanocytometry using tension-activated cell tagging Open
View article: Enhancing the effectiveness of γδ T cells by mRNA transfection of chimeric antigen receptors or bispecific T cell engagers
Enhancing the effectiveness of γδ T cells by mRNA transfection of chimeric antigen receptors or bispecific T cell engagers Open
Adoptive cell therapy (ACT) utilizing γδ T cells is becoming a promising option for the treatment of cancer, because it offers an off-the-shelf allogeneic product that is safe, potent, and clinically effective. Approaches to engineer or en…
View article: Molecular Mechanocytometry Using Tension-activated Cell Tagging (TaCT)
Molecular Mechanocytometry Using Tension-activated Cell Tagging (TaCT) Open
Flow cytometry is routinely used to measure single-cell gene expression by staining cells with fluorescent antibodies and nucleic acids. Here we present Tension-activated Cell Tagging (TaCT) to fluorescently label cells based on the magnit…
View article: Mechanically Triggered Hybridization Chain Reaction
Mechanically Triggered Hybridization Chain Reaction Open
Cells transmit piconewton forces to receptors to mediate processes such as migration and immune recognition. A major challenge in quantifying such forces is the sparsity of cell mechanical events. Accordingly, molecular tension is typicall…
View article: DNA‐Based Microparticle Tension Sensors (μTS) for Measuring Cell Mechanics in Non‐planar Geometries and for High‐Throughput Quantification
DNA‐Based Microparticle Tension Sensors (μTS) for Measuring Cell Mechanics in Non‐planar Geometries and for High‐Throughput Quantification Open
Mechanotransduction, the interplay between physical and chemical signaling, plays vital roles in many biological processes. The state‐of‐the‐art techniques to quantify cell forces employ deformable polymer films or molecular probes tethere…
View article: Dual role of endothelial <i>Myct1</i> in tumor angiogenesis and tumor immunity
Dual role of endothelial <i>Myct1</i> in tumor angiogenesis and tumor immunity Open
Myct1 inhibition controls tumor angiogenesis, remodels tumor immunity, and improves immunotherapy outcomes in mouse tumor models.
View article: Significant differences in single-platelet biophysics exist across species but attenuate during clot formation
Significant differences in single-platelet biophysics exist across species but attenuate during clot formation Open
Key Points Human, canine, ovine, and porcine platelets exhibit disparate biophysical signatures, whereas human and murine platelets are similar. Multiple biophysical parameters integrate during clot formation, measured by bulk clot contrac…
View article: Integrin affinity modulation critically regulates atherogenic endothelial activation in vitro and in vivo
Integrin affinity modulation critically regulates atherogenic endothelial activation in vitro and in vivo Open
View article: Talin-dependent integrin activation is required for endothelial proliferation and postnatal angiogenesis
Talin-dependent integrin activation is required for endothelial proliferation and postnatal angiogenesis Open
View article: Live-cell super-resolved PAINT imaging of piconewton cellular traction forces
Live-cell super-resolved PAINT imaging of piconewton cellular traction forces Open
View article: Topological Adaptation of Transmembrane Domains to the Force-Modulated Lipid Bilayer Is a Basis of Sensing Mechanical Force
Topological Adaptation of Transmembrane Domains to the Force-Modulated Lipid Bilayer Is a Basis of Sensing Mechanical Force Open
View article: Topological Adaptation of Transmembrane Domains to the Force-Modulated Lipid Bilayer Is a Basis of Sensing Mechanical Force
Topological Adaptation of Transmembrane Domains to the Force-Modulated Lipid Bilayer Is a Basis of Sensing Mechanical Force Open
View article: Integrin Affinity Modulation Critically Regulates Atherogenic Endothelial Activation<i>in vitro</i>and<i>in vivo</i>
Integrin Affinity Modulation Critically Regulates Atherogenic Endothelial Activation<i>in vitro</i>and<i>in vivo</i> Open
While vital to platelet and leukocyte adhesion, the role of integrin affinity modulation in adherent cells remains controversial. In endothelial cells, atheroprone hemodynamics and oxidized lipoproteins drive an increase in the high affini…
View article: Integrin-dependent regulation of the endothelial barrier
Integrin-dependent regulation of the endothelial barrier Open
The endothelium physically separates blood from surrounding tissue and yet allows for the regulated passage of nutrients, waste, and leukocytes into and out of the circulation. Trans-endothelium flux occurs across endothelial cells (transc…
View article: Integrin Activation Controls Regulatory T Cell–Mediated Peripheral Tolerance
Integrin Activation Controls Regulatory T Cell–Mediated Peripheral Tolerance Open
Maintenance of the regulatory T (Treg) cell pool is essential for peripheral tolerance and prevention of autoimmunity. Integrins, heterodimeric transmembrane proteins consisting of α and β subunits that mediate cell-to-cell and cell-to-ext…
View article: Integrin αvβ3 enhances the suppressive effect of interferon‐γ on hematopoietic stem cells
Integrin αvβ3 enhances the suppressive effect of interferon‐γ on hematopoietic stem cells Open
View article: Talin Plays a Critical Role in the Maintenance of the Regulatory T Cell Pool
Talin Plays a Critical Role in the Maintenance of the Regulatory T Cell Pool Open
Talin, a cytoskeletal protein essential in mediating integrin activation, has been previously shown to be involved in the regulation of T cell proliferation and function. In this study, we describe a role for talin in maintaining the homeo…
View article: Blocking neutrophil integrin activation prevents ischemia–reperfusion injury
Blocking neutrophil integrin activation prevents ischemia–reperfusion injury Open
Neutrophil recruitment, mediated by β2 integrins, combats pyogenic infections but also plays a key role in ischemia–reperfusion injury and other inflammatory disorders. Talin induces allosteric rearrangements in integrins that increase aff…
View article: Caspase-1–mediated pathway promotes generation of thromboinflammatory microparticles
Caspase-1–mediated pathway promotes generation of thromboinflammatory microparticles Open
Extracellular ATP is a signal of tissue damage and induces macrophage responses that amplify inflammation and coagulation. Here we demonstrate that ATP signaling through macrophage P2X7 receptors uncouples the thioredoxin (TRX)/TRX reducta…