Brian Stramer
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View article: Single-cell RNA sequencing clarifies dermal fibroblast subset representation <i>in vitro</i> and reveals variable persistence of keloid disease-associated features
Single-cell RNA sequencing clarifies dermal fibroblast subset representation <i>in vitro</i> and reveals variable persistence of keloid disease-associated features Open
In vitro models of scarring and fibrosis are essential to improve our understanding of disease mechanisms and ultimately develop much-needed effective therapeutic strategies. This is particularly true for keloids, the example of pathologic…
View article: Drosophila Col4a1 Glycine mutations highlight allelic heterogeneity and mechanistic pleiotropy
Drosophila Col4a1 Glycine mutations highlight allelic heterogeneity and mechanistic pleiotropy Open
Collagen IV (Col4) is a heterotrimer containing a triple helical domain broken up by short interruptions. Mutation of Glycine residues within the Glycine-X-Y triple helical repeat leads to genetically dominant disease in humans that affect…
View article: Derivation and simulation of a computational model of active cell populations: How overlap avoidance, deformability, cell-cell junctions and cytoskeletal forces affect alignment
Derivation and simulation of a computational model of active cell populations: How overlap avoidance, deformability, cell-cell junctions and cytoskeletal forces affect alignment Open
Collective alignment of cell populations is a commonly observed phenomena in biology. An important example are aligning fibroblasts in healthy or scar tissue. In this work we derive and simulate a mechanistic agent-based model of the colle…
View article: Basement membrane dynamics in living animals: Insights and pitfalls.
Basement membrane dynamics in living animals: Insights and pitfalls. Open
Recent studies with fluorophore-tagged basement membrane (BM) components have led to remarkable discoveries about BMs but also inconsistent interpretations. Here, we review types of BM dynamics, discuss how we conduct and interpret fluorop…
View article: Characterisation and correction of polarisation effects in fluorescently labelled fibres
Characterisation and correction of polarisation effects in fluorescently labelled fibres Open
Summary Many biological structures take the form of fibres and filaments, and quantitative analysis of fibre organisation is important for understanding their functions in both normal physiological conditions and disease. In order to visua…
View article: Derivation and simulation of a computational model of active cell populations: How overlap avoidance, deformability, cell-cell junctions and cytoskeletal forces affect alignment
Derivation and simulation of a computational model of active cell populations: How overlap avoidance, deformability, cell-cell junctions and cytoskeletal forces affect alignment Open
Collective alignment of cell populations is a commonly observed phenomena in biology. An important example are aligning fibroblasts in healthy or scar tissue. In this work we derive and simulate a mechanistic agent-based model of the colle…
View article: Convergent insulin and <scp>TGF</scp> ‐β signalling drives cancer cachexia by promoting aberrant fat body <scp>ECM</scp> accumulation in a <i>Drosophila</i> tumour model
Convergent insulin and <span>TGF</span> ‐β signalling drives cancer cachexia by promoting aberrant fat body <span>ECM</span> accumulation in a <i>Drosophila</i> tumour model Open
In this study, we found that in the adipose tissue of wildtype animals, insulin and TGF‐β signalling converge via a BMP antagonist short gastrulation (sog) to regulate ECM remodelling. In tumour bearing animals, Sog also modulates TGF‐β si…
View article: Characterisation and correction of polarisation effects in fluorescently labelled fibres
Characterisation and correction of polarisation effects in fluorescently labelled fibres Open
Many biological structures take the form of fibres and filaments, and quantitative analysis of fibre organisation is important for understanding their functions in both normal physiological conditions and disease. In order to visualise the…
View article: The extracellular matrix in tissue morphogenesis: No longer a backseat driver
The extracellular matrix in tissue morphogenesis: No longer a backseat driver Open
The forces driving tissue morphogenesis are thought to originate from cellular activities. While it is appreciated that extracellular matrix (ECM) may also be involved, ECM function is assumed to be simply instructive in modulating the cel…
View article: Autocrine IL-6 drives cell and extracellular matrix anisotropy in scar fibroblasts
Autocrine IL-6 drives cell and extracellular matrix anisotropy in scar fibroblasts Open
Fibrosis is associated with dramatic changes in extracellular matrix (ECM) architecture of unknown etiology. Here we exploit keloid scars as a paradigm to understand fibrotic ECM organization. We reveal that keloid patient fibroblasts uniq…
View article: Convergent Insulin and TGF-β signalling drives cancer cachexia by promoting aberrant fatbody ECM accumulation in a<i>Drosophila</i>tumour model
Convergent Insulin and TGF-β signalling drives cancer cachexia by promoting aberrant fatbody ECM accumulation in a<i>Drosophila</i>tumour model Open
Cancer cachexia is a wasting disease suffered by advanced stage cancer patients and ultimately causes ∼30% of cancer mortalities. Clinical observations have shown that extracellular matrix (ECM) remodelling which leads to fibrosis in the a…
View article: GliaMorph: a modular image analysis toolkit to quantify Müller glial cell morphology
GliaMorph: a modular image analysis toolkit to quantify Müller glial cell morphology Open
Cell morphology is crucial for all cell functions. This is particularly true for glial cells as they rely on complex shape to contact and support neurons. However, methods to quantify complex glial cell shape accurately and reproducibly ar…
View article: GliaMorph: A modular image analysis toolkit to quantify Müller glial cell morphology
GliaMorph: A modular image analysis toolkit to quantify Müller glial cell morphology Open
Cell morphology is critical for all cell functions. This is particularly true for glial cells as they rely on their complex shape to contact and support neurons. However, methods to quantify complex glial cell shape accurately and reproduc…
View article: Extracellular matrix assembly stress drives <i>Drosophila</i> central nervous system morphogenesis
Extracellular matrix assembly stress drives <i>Drosophila</i> central nervous system morphogenesis Open
Summary The forces controlling tissue morphogenesis are attributed to cellular-driven activities and any role for extracellular matrix (ECM) is assumed to be passive. However, all polymer networks, including ECM, can theoretically develop …
View article: A Workflow for Rapid Unbiased Quantification of Fibrillar Feature Alignment in Biological Images
A Workflow for Rapid Unbiased Quantification of Fibrillar Feature Alignment in Biological Images Open
Measuring the organization of the cellular cytoskeleton and the surrounding extracellular matrix (ECM) is currently of wide interest as changes in both local and global alignment can highlight alterations in cellular functions and material…
View article: Nance-Horan Syndrome-like 1 protein negatively regulates Scar/WAVE-Arp2/3 activity and inhibits lamellipodia stability and cell migration
Nance-Horan Syndrome-like 1 protein negatively regulates Scar/WAVE-Arp2/3 activity and inhibits lamellipodia stability and cell migration Open
Cell migration is important for development and its aberrant regulation contributes to many diseases. The Scar/WAVE complex is essential for Arp2/3 mediated lamellipodia formation during mesenchymal cell migration and several coinciding si…
View article: A workflow for rapid unbiased quantification of fibrillar feature alignment in biological images
A workflow for rapid unbiased quantification of fibrillar feature alignment in biological images Open
Measuring the organisation of the cellular cytoskeleton and the surrounding extracellular matrix (ECM) is currently of wide interest as changes in both local and global alignment can highlight alterations in cellular functions and material…
View article: The glycocalyx affects the mechanotransductive perception of the topographical microenvironment
The glycocalyx affects the mechanotransductive perception of the topographical microenvironment Open
The cell/microenvironment interface is the starting point of integrin-mediated mechanotransduction, but many details of mechanotransductive signal integration remain elusive due to the complexity of the involved (extra)cellular structures,…
View article: Protocol for intervention-free quantification of protein turnover rate by steady-state modeling
Protocol for intervention-free quantification of protein turnover rate by steady-state modeling Open
Protein turnover rate is difficult to obtain experimentally. This protocol shows how to mathematically model turnover rates in an intervention-free manner given the ability to quantify mRNA and protein expression from initiation to homeost…
View article: Rapid Homeostatic Turnover of Embryonic ECM during Tissue Morphogenesis
Rapid Homeostatic Turnover of Embryonic ECM during Tissue Morphogenesis Open
The extracellular matrix (ECM) is a polymer network hypothesized to form a stable cellular scaffold. While the ECM can undergo acute remodeling during embryogenesis, it is experimentally difficult to determine whether basal turnover is als…
View article: Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection
Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection Open
In this paper, a novel method for interaction detection is presented to compare the contact dynamics of macrophages in the Drosophila embryo. The study is carried out by a framework called macrosight, which analyses the movement and intera…
View article: Nance-Horan Syndrome-like 1 protein negatively regulates Scar/WAVE-Arp2/3 activity and inhibits lamellipodia stability and cell migration
Nance-Horan Syndrome-like 1 protein negatively regulates Scar/WAVE-Arp2/3 activity and inhibits lamellipodia stability and cell migration Open
Cell migration is important for development and its aberrant regulation contributes to many diseases. The Scar/WAVE complex is essential for Arp2/3 mediated lamellipodia formation during mesenchymal cell migration and several coinciding si…
View article: Fluorescent Macrophages in Drosophila Embryo
Fluorescent Macrophages in Drosophila Embryo Open
This is a data set that contains a time sequence of fluorescently labelled macrophages that migrated in a drosophila embryo. The macrophages were visualised in the embryo by using the UAS/GAL4 system.We used the srpHemo-Gal4driver,which me…
View article: Comparative study of contact repulsion in control and mutant macrophages using a novel interaction detection
Comparative study of contact repulsion in control and mutant macrophages using a novel interaction detection Open
This paper compares the contact-repulsion movement of mutant and wild-type macrophages using a novel interaction detection mechanism. The migrating macrophages are observed in Drosophila embryos. The study is carried out by a framework cal…
View article: Heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations
Heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations Open
Interactions between different cell types can induce distinct contact inhibition of locomotion (CIL) responses that are hypothesised to control population-wide behaviours during embryogenesis. However, our understanding of the signals that…