Bruce Pawel
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View article: Pediatric Embryonal Rhabdomyosarcoma: An Integrated Study of Clinicopathological Features, Pan-cancer Targeted Next-generation Sequencing, and Chromosomal Microarray Analysis from a Single Institution
Pediatric Embryonal Rhabdomyosarcoma: An Integrated Study of Clinicopathological Features, Pan-cancer Targeted Next-generation Sequencing, and Chromosomal Microarray Analysis from a Single Institution Open
Rhabdomyosarcoma is the most common soft tissue sarcoma in children and adolescents, and embryonal rhabdomyosarcoma (ERMS) is the most common subtype. Previous reports have identified a wide range of genetic aberrations in ERMS. However, t…
View article: A Retrospective Review of Autopsy Data From 6 North American Institutions Indicates Practice Variations That Could Be Leveraged to Address Declining Autopsy Rates
A Retrospective Review of Autopsy Data From 6 North American Institutions Indicates Practice Variations That Could Be Leveraged to Address Declining Autopsy Rates Open
View article: Preoperative Radiotherapy in Patients With Localized Ewing Sarcoma Enrolled on AEWS1031: A Report From the Children's Oncology Group
Preoperative Radiotherapy in Patients With Localized Ewing Sarcoma Enrolled on AEWS1031: A Report From the Children's Oncology Group Open
Patients with resectable Ewing sarcoma (ES) in select sites, such as the pelvis or chest wall, have a higher risk of positive microscopic margins following surgery. AEWS1031 was the first North American/Australasia ES cooperative group tri…
View article: Nuclear factor-κB activation by transforming growth factor-β1 drives tumour microenvironment-mediated drug resistance in neuroblastoma
Nuclear factor-κB activation by transforming growth factor-β1 drives tumour microenvironment-mediated drug resistance in neuroblastoma Open
View article: Histone H3 trimethylation on lysine 27 immunostaining pattern in DICER1-associated tumors
Histone H3 trimethylation on lysine 27 immunostaining pattern in DICER1-associated tumors Open
H3K27me3 expression is not universally lost in DICER1-associated tumors and thus is not predictive of DICER1 mutation status. The mosaic regional loss of H3K27me3 immunostaining is consistent in PPB type II and III, which can…
View article: A proteogenomic surfaceome study identifies DLK1 as an immunotherapeutic target in neuroblastoma
A proteogenomic surfaceome study identifies DLK1 as an immunotherapeutic target in neuroblastoma Open
Summary Cancer immunotherapies have produced remarkable results in B-cell malignancies; however, optimal cell surface targets for many solid cancers remain elusive. Here, we present an integrative proteomic, transcriptomic, and epigenomic …
View article: Pediatric Sertoli-Leydig Cell Tumors of the Ovary
Pediatric Sertoli-Leydig Cell Tumors of the Ovary Open
Sertoli-Leydig cell tumors (SLCTs) are currently classified into 3 molecular subtypes: DICER1 -mutant (younger patient age), FOXL2 -mutant, and DICER1/FOXL2 -wildtype. However, it is not clear whether all pediatric SLCTs are DICER1 -mutant…
View article: Norepinephrine transporter and vesicular monoamine transporter 2 tumor expression as a predictor of response to <sup>131</sup>I‐MIBG in patients with relapsed/refractory neuroblastoma
Norepinephrine transporter and vesicular monoamine transporter 2 tumor expression as a predictor of response to <sup>131</sup>I‐MIBG in patients with relapsed/refractory neuroblastoma Open
Background Prior studies suggest that norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) mediate meta‐iodobenzylguanidine (MIBG) uptake and retention in neuroblastoma tumors. We evaluated the relationship betwee…
View article: Use of Surgical Adjuvants Does Not Decrease Recurrence of Aneurysmal Bone Cysts in Surgical Intervention With Pediatric Patients
Use of Surgical Adjuvants Does Not Decrease Recurrence of Aneurysmal Bone Cysts in Surgical Intervention With Pediatric Patients Open
Background: Aneurysmal bone cysts (ABCs) are benign active tumors often requiring intralesional curettage with or without adjuvants. The primary aim of this study was to analyze whether recurrence is influenced by the use of surgical adjuv…
View article: Long-Term Outcomes in Patients With Localized Ewing Sarcoma Treated With Interval-Compressed Chemotherapy on Children's Oncology Group Study AEWS0031
Long-Term Outcomes in Patients With Localized Ewing Sarcoma Treated With Interval-Compressed Chemotherapy on Children's Oncology Group Study AEWS0031 Open
Clinical trials frequently include multiple end points that mature at different times. The initial report, typically based on the primary end point, may be published when key planned coprimary or secondary analyses are not yet available. C…
View article: Supplementary Figure 5 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 5 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
Kelly cells stably expressing non-targeting shRNA (shNT) or SHMT2 shRNA (shSHMT2) were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 48 hours incubated with glucose concentrations indicated, % cell viability was determined using Trypan Bl…
View article: Supplementary Figures 1-10; Supplemental Tables 1-6; and Supplemental Materials and Methods from Targeting PARP-1 with Alpha-Particles Is Potently Cytotoxic to Human Neuroblastoma in Preclinical Models
Supplementary Figures 1-10; Supplemental Tables 1-6; and Supplemental Materials and Methods from Targeting PARP-1 with Alpha-Particles Is Potently Cytotoxic to Human Neuroblastoma in Preclinical Models Open
SI Figure 1: Reaction scheme for synthesizing 1-(4-astatophenyl)-8,9-dihydro-2,7,9a-triazabenzo[cd]azulen-6(7H)-one ([211At]MM4) from tin precursor 1-(4-(tributylstannyl)phenyl)-8,9-dihydro-2,7,9a-triazabenzo[cd]azulen-6(7H)-one; SI Figure…
View article: Supplementary Figure 1A from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 1A from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
The correlation of SHMT2 and PHGDH expression in the samples from patients who died from the disease (top panel, pPHGDHvsSHMT1=0.092, pPHGDHvsSHMT2=7.3×10-5) and the correlation found in the samples from patients where the tumor regressed…
View article: Data from Targeting PARP-1 with Alpha-Particles Is Potently Cytotoxic to Human Neuroblastoma in Preclinical Models
Data from Targeting PARP-1 with Alpha-Particles Is Potently Cytotoxic to Human Neuroblastoma in Preclinical Models Open
Alpha-emitters can be pharmacologically delivered for irradiation of single cancer cells, but cellular lethality could be further enhanced by targeting alpha-emitters directly to the nucleus. PARP-1 is a druggable protein in the nucleus th…
View article: Supplementary Figure 5 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 5 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
Kelly cells stably expressing non-targeting shRNA (shNT) or SHMT2 shRNA (shSHMT2) were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 48 hours incubated with glucose concentrations indicated, % cell viability was determined using Trypan Bl…
View article: Supplementary Figure 1B from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 1B from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
Kaplan-Meier survival analysis in human breast cancer patients.
View article: Supplementary Table 1 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Table 1 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
Knockdown of SHMT2 reduces xenograft growth in vivo.
View article: Supplementary Table 1 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Table 1 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
Knockdown of SHMT2 reduces xenograft growth in vivo.
View article: Supplementary Figure 4 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 4 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
(A) Kelly cells stably expressing non-targeting shRNA (shNT) or SHMT2 shRNA (shSHMT2) were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 24 hours. Total cellular DNA were extracted and relative mitochondria/nuclear DNA ratio were measured…
View article: Supplementary Figure 4 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 4 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
(A) Kelly cells stably expressing non-targeting shRNA (shNT) or SHMT2 shRNA (shSHMT2) were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 24 hours. Total cellular DNA were extracted and relative mitochondria/nuclear DNA ratio were measured…
View article: Data from Targeting PARP-1 with Alpha-Particles Is Potently Cytotoxic to Human Neuroblastoma in Preclinical Models
Data from Targeting PARP-1 with Alpha-Particles Is Potently Cytotoxic to Human Neuroblastoma in Preclinical Models Open
Alpha-emitters can be pharmacologically delivered for irradiation of single cancer cells, but cellular lethality could be further enhanced by targeting alpha-emitters directly to the nucleus. PARP-1 is a druggable protein in the nucleus th…
View article: Supplementary Figure 2 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 2 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
The hypoxic induction of SHMT2 depends on HIF-1alpha. SK-N-BE(2) cells stably expressing non-targeting shRNA or a shRNA against HIF-1alpha were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 8 hours. mRNA levels were measured using Q-PCR a…
View article: Data from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Data from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
The de novo synthesis of the nonessential amino acid serine is often upregulated in cancer. In this study, we demonstrate that the serine catabolic enzyme, mitochondrial serine hydroxymethyltransferase (SHMT2), is induced when MYC-t…
View article: Supplementary Figure 3 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 3 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
(A) Immunoblots of N-Myc and c-Myc in the six cell lines. (B, C) H293T and HT1080 cells were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 6 or 16 hours, (D) H1299 cells were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 16 hours. mRNA l…
View article: Supplementary Figure 2 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 2 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
The hypoxic induction of SHMT2 depends on HIF-1alpha. SK-N-BE(2) cells stably expressing non-targeting shRNA or a shRNA against HIF-1alpha were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 8 hours. mRNA levels were measured using Q-PCR a…
View article: Supplementary Figure 1A from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 1A from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
The correlation of SHMT2 and PHGDH expression in the samples from patients who died from the disease (top panel, pPHGDHvsSHMT1=0.092, pPHGDHvsSHMT2=7.3×10-5) and the correlation found in the samples from patients where the tumor regressed…
View article: Data from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Data from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
The de novo synthesis of the nonessential amino acid serine is often upregulated in cancer. In this study, we demonstrate that the serine catabolic enzyme, mitochondrial serine hydroxymethyltransferase (SHMT2), is induced when MYC-t…
View article: Supplementary Figure 3 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 3 from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
(A) Immunoblots of N-Myc and c-Myc in the six cell lines. (B, C) H293T and HT1080 cells were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 6 or 16 hours, (D) H1299 cells were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 16 hours. mRNA l…
View article: Supplementary Figure 1B from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Supplementary Figure 1B from Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia Open
Kaplan-Meier survival analysis in human breast cancer patients.
View article: Lorlatinib with or without chemotherapy in ALK-driven refractory/relapsed neuroblastoma: phase 1 trial results
Lorlatinib with or without chemotherapy in ALK-driven refractory/relapsed neuroblastoma: phase 1 trial results Open
Neuroblastomas harbor ALK aberrations clinically resistant to crizotinib yet sensitive pre-clinically to the third-generation ALK inhibitor lorlatinib. We conducted a first-in-child study evaluating lorlatinib with and without chemotherapy…