Bruno Weber
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View article: Sex-specific cardiac ageing and sympathetic compensation: a translational study
Sex-specific cardiac ageing and sympathetic compensation: a translational study Open
Background and Aims Recent evidence suggests that individuals with smaller left ventricular (LV) volumes and high LV ejection fraction face an increased risk of cardiovascular morbidity and mortality. Age-related cardiac changes may partic…
View article: APOE genotype-dependent differences in human astrocytic energy metabolism
APOE genotype-dependent differences in human astrocytic energy metabolism Open
Introduction The main genetic risk factor for Alzheimer’s disease (AD) is the presence of the apolipoprotein E4 ( APOE4 ) allele. While APOE4 increases the risk of developing AD, the APOE2 allele is protective and APOE3 is risk-neutral. In…
View article: Abundance-biased codon diversification prevents recombination in AAV production and ensures robust in vivo expression of functional FRET sensors
Abundance-biased codon diversification prevents recombination in AAV production and ensures robust in vivo expression of functional FRET sensors Open
The delivery of genetically encoded fluorescent sensors via adeno-associated viral vectors (AAVs) enables the quantification of biological analytes with high spatiotemporal resolution in living animals. In this study, we expose an unreport…
View article: In Vivo Network-Level Cerebrovascular Mapping Reveals the Impact of Flow Topology on Capillary Stalls After Stroke
In Vivo Network-Level Cerebrovascular Mapping Reveals the Impact of Flow Topology on Capillary Stalls After Stroke Open
Cerebral microvasculature is essential for brain function, but how flow and large-scale connectivity contribute to its resilience or failure remains poorly understood. To address this, we developed OMNIMap, a framework for mesoscale in viv…
View article: Embracing the Modern Biochemistry of Brain Metabolism
Embracing the Modern Biochemistry of Brain Metabolism Open
This editorial challenges the long‐held neuron‐centered view of brain metabolism, relying on ample evidence that it is a cooperative, multicellular process. Astrocytes, oligodendrocytes, and other glia play active roles providing lactate, …
View article: Coordinated Two‐Photon Fluorescence and Optoacoustic Microscopy of Neural, Vascular, and Cellular Dynamics in the Mouse Brain
Coordinated Two‐Photon Fluorescence and Optoacoustic Microscopy of Neural, Vascular, and Cellular Dynamics in the Mouse Brain Open
Imaging techniques capable of visualizing the nervous and vascular systems are essential for uncovering the fundamental mechanisms underlying brain functions. To enable visualization of both systems at the microscale with a streamlined ima…
View article: A low-cost FPGA-based approach for pile-up corrected high-speed in vivo FLIM imaging
A low-cost FPGA-based approach for pile-up corrected high-speed in vivo FLIM imaging Open
We enable high-precision, cost-effective FLIM imaging at acquisition speeds comparable with state-of-the-art commercial systems, facilitating the adoption of FLIM in neuroscience and other fields of research needing robust quantitative liv…
View article: Interleukin-34-dependent perivascular macrophages promote vascular function in the brain
Interleukin-34-dependent perivascular macrophages promote vascular function in the brain Open
The development of most macrophages depends on the colony-stimulating factor 1 (CSF-1) receptor, which has two ligands: CSF-1 and interleukin-34 (IL-34). While IL-34 is required for the homeostasis of microglia, the parenchymal macrophages…
View article: Deletion of microsomal epoxide hydrolase gene leads to increased density in cerebral vasculature and enhances cerebral blood flow in mice
Deletion of microsomal epoxide hydrolase gene leads to increased density in cerebral vasculature and enhances cerebral blood flow in mice Open
Microsomal epoxide hydrolase (mEH), first identified as detoxifying enzyme, can hydrolyze epoxyeicosatrienoic acids (EETs) to less active diols (DHETs). EETs are potent vasodilatory and pro-angiogenic lipids, also implicated in neurovascul…
View article: A low-cost FPGA-based approach for pile-up corrected high-speed in vivo FLIM imaging
A low-cost FPGA-based approach for pile-up corrected high-speed in vivo FLIM imaging Open
Intensity-based two-photon microscopy (2PM) is a cornerstone of biomedical research but lacks the ability to measure concentrations, a pivotal task for longitudinal studies and quantitative comparisons. Fluorescence Lifetime Imaging (FLIM)…
View article: Overactivation of prefrontal astrocytes impairs cognition through the metabolic pathway of central kynurenines
Overactivation of prefrontal astrocytes impairs cognition through the metabolic pathway of central kynurenines Open
Astrocyte dysfunctions have long been implicated in psychiatric and cognitive disorders, yet the precise mechanisms underlying this association remain elusive. Here, we show that chemogenetic activation of prefrontal astrocytes in mice imp…
View article: Author Correction: Oligodendroglial fatty acid metabolism as a central nervous system energy reserve
Author Correction: Oligodendroglial fatty acid metabolism as a central nervous system energy reserve Open
status: Published
View article: Oligodendroglial fatty acid metabolism as a central nervous system energy reserve
Oligodendroglial fatty acid metabolism as a central nervous system energy reserve Open
Brain function requires a constant supply of glucose. However, the brain has no known energy stores, except for glycogen granules in astrocytes. In the present study, we report that continuous oligodendroglial lipid metabolism provides an …
View article: Lactate-carried Mitochondrial Energy Overflow
Lactate-carried Mitochondrial Energy Overflow Open
We addressed the question of mitochondrial lactate metabolism using genetically-encoded sensors. The organelle was found to contain a dynamic lactate pool that leads to dose- and time-dependent protein lactylation. In neurons, mitochondria…
View article: Pia-FLOW: Deciphering hemodynamic maps of the pial vascular connectome and its response to arterial occlusion
Pia-FLOW: Deciphering hemodynamic maps of the pial vascular connectome and its response to arterial occlusion Open
The pial vasculature is the sole source of blood supply to the neocortex. The brain is contained within the skull, a vascularized bone marrow with a unique anatomical connection to the brain meninges. Recent developments in tissue clearing…
View article: Astrocytes adopt a progenitor-like migratory strategy for regeneration in adult brain
Astrocytes adopt a progenitor-like migratory strategy for regeneration in adult brain Open
Mature astrocytes become activated upon non-specific tissue damage and contribute to glial scar formation. Proliferation and migration of adult reactive astrocytes after injury is considered very limited. However, the regenerative behavior…
View article: Cortex-wide transcranial localization microscopy with fluorescently labeled red blood cells
Cortex-wide transcranial localization microscopy with fluorescently labeled red blood cells Open
Large-scale imaging of brain activity with high spatio-temporal resolution is crucial for advancing our understanding of brain function. The existing neuroimaging techniques are largely limited by restricted field of view, slow imaging spe…
View article: Bessel Beam Optical Coherence Microscopy Enables Multiscale Assessment of Cerebrovascular Network Morphology and Function
Bessel Beam Optical Coherence Microscopy Enables Multiscale Assessment of Cerebrovascular Network Morphology and Function Open
Understanding the morphology and function of large-scale cerebrovascular networks is crucial for studying brain health and disease. However, reconciling the demands for imaging on a broad scale with the precision of high-resolution volumet…
View article: L-Type Ca<sup>2+</sup>channels and TRPC3 channels shape brain pericyte Ca<sup>2+</sup>signaling and hemodynamics throughout the arteriole to capillary network<i>in vivo</i>
L-Type Ca<sup>2+</sup>channels and TRPC3 channels shape brain pericyte Ca<sup>2+</sup>signaling and hemodynamics throughout the arteriole to capillary network<i>in vivo</i> Open
Pericytes play a crucial role in regulating cerebral blood flow (CBF) through processes like vasomotion and neurovascular coupling (NVC). Recent work has identified different pericyte types at distinct points in the cerebrovascular network…
View article: Cortical astrocyte N-methyl-D-aspartate receptors influence whisker barrel activity and sensory discrimination in mice
Cortical astrocyte N-methyl-D-aspartate receptors influence whisker barrel activity and sensory discrimination in mice Open
Astrocytes express ionotropic receptors, including N-methyl-D-aspartate receptors (NMDARs). However, the contribution of NMDARs to astrocyte-neuron interactions, particularly in vivo, has not been elucidated. Here we show that a knockdown …
View article: The gephyrin scaffold modulates cortical layer 2/3 pyramidal neuron responsiveness to single whisker stimulation
The gephyrin scaffold modulates cortical layer 2/3 pyramidal neuron responsiveness to single whisker stimulation Open
Gephyrin is the main scaffolding protein at inhibitory postsynaptic sites, and its clusters are the signaling hubs where several molecular pathways converge. Post-translational modifications (PTMs) of gephyrin alter GABA A receptor cluster…
View article: Abundance-Biased Codon Diversification prevents recombination in AAV production and ensures robust in vivo expression of functional FRET sensors
Abundance-Biased Codon Diversification prevents recombination in AAV production and ensures robust in vivo expression of functional FRET sensors Open
The delivery of genetically encoded fluorescent sensors via adeno-associated viral vectors (AAVs) enables the quantification of biological analytes with high spatiotemporal resolution in living animals. In this study, we expose an unreport…