Explanipedia

ESCRT disruption provides evidence against trans-synaptic signaling via extracellular vesicles Open

Erica C. Dresselhaus, Kathryn P. Harris, Cassandra R. Blanchette, Kate Koles, Steven J. Del Signore , et al. · 2024

Extracellular vesicles (EVs) are released by many cell types, including neurons, carrying cargoes involved in signaling and disease. It is unclear whether EVs promote intercellular signaling or serve primarily to dispose of unwanted materi…

Electron microscopic analysis of the influence of iPSC-derived motor neurons on bioengineered human skeletal muscle tissues Open

Christine T. O. Nguyen, Carolina Chávez-Madero, Erik Jacques, Brennen Musgrave, Ting Yin , et al. · 2024

ESCRT disruption provides evidence against signaling functions for synaptic exosomes Open

Erica C. Dresselhaus, Kathryn P. Harris, Cassandra R. Blanchette, Kate Koles, Steven J. Del Signore , et al. · 2023

Extracellular vesicles (EVs) are released by many cell types including neurons, carrying cargoes involved in signaling and disease. It is unclear whether EVs promote intercellular signaling or serve primarily to dispose of unwanted materia…

Electron microscopic analysis of the influence of iPSC-derived motor neurons on bioengineered human skeletal muscle tissues Open

Christine T. O. Nguyen, Carolina Chávez-Madero, Erik Jacques, Brennen Musgrave, Ting Yin , et al. · 2023

3D bioengineered skeletal muscle macrotissues are increasingly important for studies of cell biology and development of therapeutics. Tissues derived from immortalized cells obtained from patient samples or from stem cells can be co-cultur…

Local regulation of extracellular vesicle traffic by the synaptic endocytic machinery Open

Cassandra R. Blanchette, Amy L. Scalera, Kathryn P. Harris, Zechuan Zhao, Erica C. Dresselhaus , et al. · 2022

Neuronal extracellular vesicles (EVs) are locally released from presynaptic terminals, carrying cargoes critical for intercellular signaling and disease. EVs are derived from endosomes, but it is unknown how these cargoes are directed to t…

Second Harmonic Generation Properties in Chiral Sarcomeres of Drosophila Larval Muscles Open

Abiramy Karunendiran, Kamdin Mirsanaye, Bryan A. Stewart, Virginijus Barzda · 2022

Polarimetric second harmonic generation (SHG) microscopy is a label-free ultrastructural imaging technique that is applied to study molecular organization in striated muscle structure. Wild type OreR and UNC45 knockdown Drosophila melanoga…

Electrophysiological analysis of healthy and dystrophic 3-D bioengineered skeletal muscle tissues Open

Christine T. O. Nguyen, Majid Ebrahimi, Penney M. Gilbert, Bryan A. Stewart · 2021

Recently, methods for creating three-dimensional (3-D) human skeletal muscle tissues from myogenic cell lines have been reported. Bioengineered muscle tissues are contractile and respond to electrical and chemical stimulation. In this stud…

Local regulation of extracellular vesicle traffic by the synaptic endocytic machinery Open

Cassandra R. Blanchette, Amy L. Scalera, Kathryn P. Harris, Zechuan Zhao, Kate Koles , et al. · 2021

Neuronal extracellular vesicles (EVs) can be locally released from presynaptic terminals, carrying cargoes that are important in intercellular signaling and disease. EVs are derived from endosomes, but it remains unclear how synaptic cargo…

Disruption of Drosophila larval muscle structure and function by UNC45 knockdown Open

Abiramy Karunendiran, Christine T. O. Nguyen, Virginijus Barzda, Bryan A. Stewart · 2021

Electrophysiological analysis of healthy and dystrophic 3D bioengineered skeletal muscle tissues Open

Christine T. O. Nguyen, Majid Ebrahmi, Penney M. Gilbert, Bryan A. Stewart · 2020

Recently, methods for creating three-dimensional (3D) human skeletal muscle tissues from myogenic cell lines have been reported. Bioengineered muscle tissues are contractile and respond to electrical and chemical stimulation. In this study…

Dual‐ and single‐shot susceptibility ratio measurements with circular polarizations in second‐harmonic generation microscopy Open

Ahmad Golaraei, Lukas Kontenis, Abiramy Karunendiran, Bryan A. Stewart, Virginijus Barzda · 2020

Polarization‐resolved second‐harmonic generation (P‐SHG) microscopy is a technique capable of characterizing nonlinear optical properties of noncentrosymmetric biomaterials by extracting the nonlinear susceptibility tensor components ratio…

The neuronal ceroid lipofuscinosis protein Cln7 functions in the postsynaptic cell to regulate synapse development Open

Kyle J. Connolly, Megan B O'Hare, Alamin Mohammed, Katelyn M. Aitchison, Niki Anthoney , et al. · 2019

Live imaging of contracting muscles with wide-field second harmonic generation microscopy using a high power laser Open

Haitao Zhao, Richard Cisek, Abiramy Karunendiran, Danielle Tokarz, Bryan A. Stewart , et al. · 2019

Wide-field second harmonic generation (SHG) microscopy was developed using a high-power (> 4 W) and high-repetition-rate (MHz range) laser oscillator to achieve fast SHG imaging over a large area (400 µm × 400 µm). The microscope was used …

Issue Information Open

Eduardo R. Macagno, Darcy B. Kelley, J. W. Fawcett, Moses V. Chao, Carlos D. Aizenman , et al. · 2019

Wiley's Corporate Citizenship initiative seeks to address the environmental, social, economic, and ethical challenges faced in our business and which are important to our diverse stakeholder groups.Since launching the initiative, we have f…

A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction Open

Mohsen Afshar Bakooshli, Ethan S. Lippmann, Ben Mulcahy, Nisha Iyer, Christine T. O. Nguyen , et al. · 2019

Two-dimensional (2D) human skeletal muscle fiber cultures are ill-equipped to support the contractile properties of maturing muscle fibers. This limits their application to the study of adult human neuromuscular junction (NMJ) development,…

Author response: A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction Open

Mohsen Afshar Bakooshli, Ethan S. Lippmann, Ben Mulcahy, Nisha Iyer, Christine T. O. Nguyen , et al. · 2019

Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Two-dimensional (2D) human skeletal muscle…

Regulation of SH3PX1 by dNedd4-long at the Drosophila neuromuscular junction Open

Samantha S. Wasserman, Alina Shteiman-Kotler, Kathryn P. Harris, Konstantin G. Iliadi, Avinash K. Persaud , et al. · 2018

Drosophila Nedd4 (dNedd4) is a HECT E3 ubiquitin ligase present in two major isoforms: short (dNedd4S) and long (dNedd4Lo), with the latter containing two unique regions (N terminus and Middle). Although dNedd4S promotes neuromuscul…

Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release Open

Kathryn P. Harris, J. Troy Littleton, Bryan A. Stewart · 2018

Signaling from the postsynaptic compartment regulates multiple aspects of synaptic development and function. Syntaxin 4 (Syx4) is a plasma membrane t-SNARE that promotes the growth and plasticity of Drosophila neuromuscular junction…

The neuronal ceroid lipofuscinosis protein Cln7 regulates neural development from the post-synaptic cell Open

Kyle J. Connolly, Megan B O'Hare, Alamin Mohammed, Katelyn M. Aitchison, Niki Anthoney , et al. · 2018

The neuronal ceroid lipofuscinoses (NCLs) are a group of fatal, monogenic neurodegenerative disorders with an early onset in infancy or childhood. Despite identification of the genes disrupted in each form of the disease, their normal cell…

Jack bean urease modulates neurotransmitter release at insect neuromuscular junctions Open

Thiago Carrazoni, Christine T. O. Nguyen, Lucas F. Maciel, Andrés Delgado Cañedo, Bryan A. Stewart , et al. · 2018

JBU (0.28 mg/animal/day) in Locusta migratoria 2nd instar through feeding and injection did not induce lethality, although it did result in a reduction of 20% in the weight gain at the end of 168 h (n = 9, p ≤ 0.05). JBU (0.014 and 0.14 mg…

A three-dimensional culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction and disease modeling Open

Mohsen Afshar Bakooshli, Ethan S. Lippmann, Ben Mulcahy, Nisha Iyer, Christine T. O. Nguyen , et al. · 2018

Summary Two-dimensional (2D) human skeletal muscle fiber cultures are ill equipped to support the contractile properties of maturing muscle fibers. This limits their application to the study of adult human neuromuscular junction (NMJ) deve…

Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release Open

Kathryn P. Harris, J. Troy Littleton, Bryan A. Stewart · 2018

Signaling from the postsynaptic compartment regulates multiple aspects of synaptic development and function. Syntaxin 4 (Syx4) is a plasma membrane t-SNARE that promotes the growth and plasticity of Drosophila neuromuscular junction…

Examination of Drosophila eye development with third harmonic generation microscopy Open

Abiramy Karunendiran, Richard Cisek, Danielle Tokarz, Virginijus Barzda, Bryan A. Stewart · 2017

Third harmonic generation (THG) microscopy can exploit endogenous harmonophores such as pigment macromolecules for enhanced image contrast, and therefore can be used without exogenous contrast agents. Previous studies have established that…

The influence of postsynaptic structure on missing quanta at the Drosophila neuromuscular junction Open

Christine T. O. Nguyen, Bryan A. Stewart · 2016

Second harmonic generation double stokes Mueller polarimetric microscopy of myofilaments Open

Lukas Kontenis, Masood Samim, Abiramy Karunendiran, Serguei Krouglov, Bryan A. Stewart , et al. · 2016

The experimental implementation of double Stokes Mueller polarimetric microscopy is presented. This technique enables a model-independent and complete polarimetric characterization of second harmonic generating samples using 36 Stokes para…

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