William Chrisler
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View article: Improved surfactant-assisted one-pot sample preparation for robust convenient single cell proteomics
Improved surfactant-assisted one-pot sample preparation for robust convenient single cell proteomics Open
Recent advances in mass spectrometry-based single-cell proteomics (SCP) technologies have revolutionized the SCP field for comprehensive characterization of cellular heterogeneity. However, most of the current SCP approaches employ sub-µL …
View article: Sorted-cell proteomics reveals an AT1-associated epithelial cornification phenotype and suggests endothelial redox imbalance in human bronchopulmonary dysplasia
Sorted-cell proteomics reveals an AT1-associated epithelial cornification phenotype and suggests endothelial redox imbalance in human bronchopulmonary dysplasia Open
The study is the first to perform proteomics on sorted pulmonary epithelial and endothelial populations from bronchopulmonary dysplasia (BPD) and age-matched control human donors. We identified an increase in cornification-associated prote…
View article: Activity-targeted metaproteomics uncovers rare syntrophic bacteria central to anaerobic community metabolism
Activity-targeted metaproteomics uncovers rare syntrophic bacteria central to anaerobic community metabolism Open
Syntrophic microbial consortia can contribute significantly to the activity and function of anoxic ecosystems, yet are often too rare to study their in situ physiologies using traditional molecular methods. Here, we combined bioorthogonal …
A Molecular Velcro Self-Healing Cement Open
Cement, a fundamental component in concrete infrastructure, is inherently susceptible to cracking and degradation. Conventional self-healing strategies—whether intrinsic or extrinsic—often suffer from limited healing agent availability, re…
View article: Sorted-Cell Proteomics Reveals an AT1-Associated Epithelial Cornification Phenotype and Suggests Endothelial Redox Imbalance in Human Bronchopulmonary Dysplasia
Sorted-Cell Proteomics Reveals an AT1-Associated Epithelial Cornification Phenotype and Suggests Endothelial Redox Imbalance in Human Bronchopulmonary Dysplasia Open
Bronchopulmonary dysplasia (BPD) is a neonatal lung disease characterized by inflammation and scarring leading to long-term tissue damage. Previous whole tissue proteomics identified BPD-specific proteome changes and cell type shifts. Litt…
View article: Parallel measurement of transcriptomes and proteomes from same single cells using nanodroplet splitting
Parallel measurement of transcriptomes and proteomes from same single cells using nanodroplet splitting Open
Single-cell multiomics provides comprehensive insights into gene regulatory networks, cellular diversity, and temporal dynamics. Here, we introduce nanoSPLITS (nanodroplet SPlitting for Linked-multimodal Investigations of Trace Samples), a…
View article: Carrier-assisted One-pot Sample Preparation for Targeted Proteomics Analysis of Small Numbers of Human Cells v2
Carrier-assisted One-pot Sample Preparation for Targeted Proteomics Analysis of Small Numbers of Human Cells v2 Open
Protein analysis of small numbers of human cells is primarily achieved by targeted proteomics with antibody-based immunoassays, whereas they have inherent limitations (e.g., low multiplex and unavailability of antibodies for new proteins).…
View article: Cell‐type‐specific transcriptomics uncovers spatial regulatory networks in bioenergy sorghum stems
Cell‐type‐specific transcriptomics uncovers spatial regulatory networks in bioenergy sorghum stems Open
SUMMARY Bioenergy sorghum is a low‐input, drought‐resilient, deep‐rooting annual crop that has high biomass yield potential enabling the sustainable production of biofuels, biopower, and bioproducts. Bioenergy sorghum's 4–5 m stems account…