Catherine Fenselau
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View article: Mamoru Ohashi: Some Personal Memories
Mamoru Ohashi: Some Personal Memories Open
was a highly creative scientist and an inuential leader in several scienti c elds, however I always re-
View article: Extraction of Membrane Components from Neisseria gonorrhoeae Using Catanionic Surfactant Vesicles: A New Approach for the Study of Bacterial Surface Molecules
Extraction of Membrane Components from Neisseria gonorrhoeae Using Catanionic Surfactant Vesicles: A New Approach for the Study of Bacterial Surface Molecules Open
Identification of antigens is important for vaccine production. We tested extraction protocols using cetyltrimethylammonium tosylate (CTAT) and sodium dodecylbenzenesulfonate (SDBS) to formulate surfactant vesicles (SVs) containing compone…
View article: Top-Down Proteomic Characterization of Truncated Proteoforms
Top-Down Proteomic Characterization of Truncated Proteoforms Open
A top-down proteomic strategy with semi-automated analysis of data sets has proven successful for global identification of truncated proteins without the use of chemical derivatization, enzymatic manipulation, immunoprecipitation or other …
View article: Top‐down analysis of novel synthetic branched proteins
Top‐down analysis of novel synthetic branched proteins Open
A strategy for top‐down analysis of branched proteins has been reported earlier, which relies on electron transfer dissociation assisted by collisional activation, and software designed for graphic interpretation of tandem mass spectra and…
View article: Rapid analysis of ricin using hot acid digestion and MALDI‐TOF mass spectrometry
Rapid analysis of ricin using hot acid digestion and MALDI‐TOF mass spectrometry Open
Ricin is a protein toxin of considerable interest in forensics. A novel strategy is reported here for rapid detection of ricin based on microwave‐assisted hot acid digestion and matrix‐assisted laser desorption/ionization‐time‐of‐flight ma…
View article: Top-Down Analysis of Branched Proteins Using Mass Spectrometry
Top-Down Analysis of Branched Proteins Using Mass Spectrometry Open
Post-translational modifications by the covalent attachment of Rub1 (NEDD8), ubiquitin, SUMO, and other small signaling proteins have profound impacts on the functions and fates of cellular proteins. Investigations of the relationship of t…
View article: How many human proteoforms are there
How many human proteoforms are there Open
Despite decades of accumulated knowledge about proteins and their post-translational modifications (PTMs), numerous questions remain regarding their molecular composition and biological function. One of the most fundamental queries is the …
View article: Myeloid-Derived Suppressor Cells: Immune-Suppressive Cells That Impair Antitumor Immunity and Are Sculpted by Their Environment
Myeloid-Derived Suppressor Cells: Immune-Suppressive Cells That Impair Antitumor Immunity and Are Sculpted by Their Environment Open
Myeloid-derived suppressor cells (MDSC) are a diverse population of immature myeloid cells that have potent immune-suppressive activity. Studies in both mice and humans have demonstrated that MDSC accumulate in most individuals with cancer…
View article: Differential Content of Proteins, mRNAs, and miRNAs Suggests that MDSC and Their Exosomes May Mediate Distinct Immune Suppressive Functions
Differential Content of Proteins, mRNAs, and miRNAs Suggests that MDSC and Their Exosomes May Mediate Distinct Immune Suppressive Functions Open
Myeloid-derived suppressor cells (MDSC) are immature myeloid cells that accumulate in the circulation and the tumor microenvironment of most cancer patients. There, MDSC suppress both adaptive and innate immunity, hindering immunotherapies…
View article: Ubiquitin Conjugation Probed by Inflammation in Myeloid-Derived Suppressor Cell Extracellular Vesicles
Ubiquitin Conjugation Probed by Inflammation in Myeloid-Derived Suppressor Cell Extracellular Vesicles Open
Ubiquitinated proteins carried by the extracellular vesicles (EV) released by myeloid-derived suppressor cells (MDSC) have been investigated using proteomic strategies to examine the effect of tumor-associated inflammation. EV were collect…
View article: Evaluation of Spectral Counting for Relative Quantitation of Proteoforms in Top-Down Proteomics
Evaluation of Spectral Counting for Relative Quantitation of Proteoforms in Top-Down Proteomics Open
Spectral counting is a straightforward label-free quantitation strategy used in bottom-up proteomics workflows. The application of spectral counting in label-free top-down proteomics workflows can be similarly straightforward but has not b…
View article: Surface Glycoproteins of Exosomes Shed by Myeloid-Derived Suppressor Cells Contribute to Function
Surface Glycoproteins of Exosomes Shed by Myeloid-Derived Suppressor Cells Contribute to Function Open
In this report, we use a proteomic strategy to identify glycoproteins on the surface of exosomes derived from myeloid-derived suppressor cells (MDSCs), and then test if selected glycoproteins contribute to exosome-mediated chemotaxis and m…
View article: Preparing to read the ubiquitin code: top‐down analysis of unanchored ubiquitin tetramers
Preparing to read the ubiquitin code: top‐down analysis of unanchored ubiquitin tetramers Open
The characterization of polyubiquitin chains has been an analytical challenge for several decades. It has been shown that anchored and unanchored polyubiquitin chains with different isopeptide linkages and lengths exhibit a wide range of p…
View article: Peptide‐based systems analysis of inflammation induced myeloid‐derived suppressor cells reveals diverse signaling pathways
Peptide‐based systems analysis of inflammation induced myeloid‐derived suppressor cells reveals diverse signaling pathways Open
A better understanding of molecular signaling between myeloid‐derived suppressor cells (MDSC), tumor cells, T‐cells, and inflammatory mediators is expected to contribute to more effective cancer immunotherapies. We focus on plasma membrane…
View article: Preparing to read the ubiquitin code: characterization of ubiquitin trimers by top‐down mass spectrometry
Preparing to read the ubiquitin code: characterization of ubiquitin trimers by top‐down mass spectrometry Open
The profound effects of ubiquitination on the movement and processing of cellular proteins depend exquisitely on the structures of monoubiquitin and polyubiquitin modifications. Unconjugated polyubiquitins also have a variety of intracellu…
View article: MOESM2 of Extracellular vesicle proteomes reflect developmental phases of Bacillus subtilis
MOESM2 of Extracellular vesicle proteomes reflect developmental phases of Bacillus subtilis Open
Additional file 2: Table S2. Differentially abundant proteins in sporulating (Spo) and vegetative (Veg) extra-cellular vesicles.
View article: MOESM1 of Extracellular vesicle proteomes reflect developmental phases of Bacillus subtilis
MOESM1 of Extracellular vesicle proteomes reflect developmental phases of Bacillus subtilis Open
Additional file 1: Table S1. Proteins identified in sporulating (Spo) and vegetative (Veg) extra-cellular vesicles.