Explanipedia

Diffusion in the aqueous compartment. Open

Andrea M. Mastro, Alec D. Keith · 1984

Measurements of diffusion of molecules in cells can provide information about cytoplasmic viscosity and structure. In a series of studies electron-spin resonance was used to measure the diffusion of a small spin label in the aqueous cytopl…

Diffusion of a small molecule in the cytoplasm of mammalian cells. Open

Andrea M. Mastro, Michael A. Babich, William D. Taylor, Alec D. Keith · 1984

Electron spin resonance was used to measure the diffusion of a small (Mr 170) spin label in the aqueous cytoplasm of mammalian cells. Translational and rotational motion were determined from the same spectra. Based on measurements made in …

Studies with a Hydrophobic, Spin-Labeled Virucidal Agent Open

Neal A. DeLuca, Alec D. Keith, Wallace Snipes · 1980

A spin-labeled virucidal agent was synthesized, purified, and tested for its activity against the enveloped bacterial virus φ6 and herpes simplex virus. This compound, designated BPN, inactivated greater than 99% of φ6 and herpes simplex v…

Factors restricting diffusion of water-soluble spin labels Open

Alec D. Keith, Wallace Snipes, Rolf J. Mehlhorn, Thomas E. Gunter · 1977

Spin Label Studies on Membranes and the Aqueous Zones of Membrane Enclosures Open

Alec D. Keith · 1977

the buried carboxyl residues of BR in the mechanism of the light-driven proton pump. In addition, these studies obtained structural information which supports current models of BR protein structure in the purple membrane.

Inositol-less death in yeast results in a simultaneous increase in intracellular viscosity Open

Alec D. Keith, E. Pollard, Wallace Snipes · 1977

Inactivation of Lipid-Containing Viruses by Long-Chain Alcohols Open

Wallace Snipes, Stanley Person, Gregory S. Keller, William D. Taylor, Alec D. Keith · 1977

This report describes the inactivation of lipid-containing viruses by several long-chain alcohols. A striking peak in antiviral activity was found for saturated alcohols having chain lengths from 10 to 14 carbons. Viruses having different …

Inactivation of the Enveloped Bacteriophage ø6 by Butylated Hydroxytoluene and Butylated Hydroxyanisole Open

Paul E. Wanda, James Cupp, Wallace Snipes, Alec D. Keith, Tom Rucinsky , et al. · 1976

Butylated hydroxytoluene (BHT) is a potent inactivator of the enveloped bacterial virus ø6 at concentrations as low as 3 × 10 −5 M. The viral envelope is not removed by BHT treatment, in contrast to the effects of exposure to the detergent…

Changes in the restriction of molecular rotational diffusion of water-soluble spin labels during fatty acid starvation of yeast Open

Susan A. Henry, Alec D. Keith, Wallace Snipes · 1976

Use of Spin Labels and Electron Spin Resonance Spectroscopy to Characterize Membranes of Bovine Sperm: Effect of Butylated Hydroxytoluene and Cold Shock Open

Roy H. Hammerstedt, R. P. Amann, Tom Rucinsky, Philip D. Morse, James R. Lepock , et al. · 1976

Spin label probes were used in conjunction with measurements of metabolic rate and electron microscopy to characterize bovine sperm membranes. Aqueous compartments, membrane hydrocarbon zones and lipid:water interfaces were studied separat…

Comprehensive report of the overall activities of the contract during the past three years. [Physical properties of biological membranes] Open

Alec D. Keith · 1976

The research accomplishments carried out during the last three years of support by ERDA are outlined. These entail various experiments that were carried out using spin labels to uncover information about the physical properties of biologic…

Inactivation of the Lipid-Containing Bacteriophage PM2 by Butylated Hydroxytoluene Open

James Cupp, Paul E. Wanda, Alec D. Keith, Wallace Snipes · 1975

Several factors have been investigated which are of significance in the inactivation of PM2, a lipid-containing bacterial virus, by butylated hydroxytoluene (BHT). Studies of the time dependence of inactivation during exposure to BHT showe…

Temperature-induced phase transitions in nematode lipids and their influence on respiration. Open

Joseph Lyons, Alec D. Keith, I. J. Thomason · 1975

Temperature-induced phase transitions estimated by electron spin resonance (ESR) technique were ohscrved in the lipids of several nematode species. In both Meloidogyne javanica and Caenorhahditis elegans, there was a phase transition in th…

Effect of Isoniazid on the Protoplasmic Viscosity in Mycobacterium tuberculosis Open

Kuni Takayama, Alec D. Keith, Wallace Snipes · 1975

The effect of isoniazid on the protoplasmic viscosity in the H37Ra strain of Mycobacterium tuberculosis was determined by using electron spin resonance spectroscopy and a small spin label tempone (2,2,6,6-tetramethylpiperidone- N -oxyl rad…

Active Transport of Choline by a Marine Pseudomonad Open

Wallace Snipes, Alec D. Keith, Paul E. Wanda · 1974

A marine pseudomonad, BAL-31, accumulates the phospholipid nitrogen base, choline, although no detectable amount of choline is incorporated into polar lipids. Metabolic inhibitors such as cyanide and azide block the uptake process as does …

Spin-labeled membranes. Progress report Open

Alec D. Keith · 1974

the buried carboxyl residues of BR in the mechanism of the light-driven proton pump. In addition, these studies obtained structural information which supports current models of BR protein structure in the purple membrane.

Spin Label Translational Diffusion in Solid Tristearin Open

Gerald E. Cohn, Alec D. Keith, Wallace Snipes · 1974

Electron Spin Resonance Analysis of the Nitroxide Spin Label 2,2,6,6-Tetramethylpiperidone-N-Oxyl (Tempone) in Single Crystals of the Reduced Tempone Matrix Open

Wallace Snipes, James Cupp, Gerald E. Cohn, Alec D. Keith · 1974

Spin Label Motion in Fatty Acids Open

Rolf J. Mehlhorn, Wallace Snipes, Alec D. Keith · 1973

Spin-labeled membranes. Progress report, November 1, 1972--September 1973 Open

Alec D. Keith · 1973

The development of unsaturated fatty acid auxotrophs as subjects for spin label studies and as a measure of getting information about biological membranes was investigated using Neurospora crassa. (HLW)

Correlations between fatty acid distribution in phospholipids and the temperature dependence of membrane physical state Open

Carol D. Linden, Alec D. Keith, Colin Fox · 1973

and GLAn-6 effects were similar and more pronounced in the depths of the membrane. The changes in the structure and dynamics of samples were obtained from simulations of spectra, which indicated some changes in the number of spectral compo…

SPIN LABELED MEMBRANES. Progress Report, November 1, 1971--August 8, 1972. Open

Alec D. Keith · 1972

Temperature-induced Phase Changes in Mitochondrial Membranes Detected by Spin Labeling Open

John K. Raison, Joseph Lyons, Rolf J. Mehlhorn, Alec D. Keith · 1971

Molecular motion data from a spin-labeled fatty acid presented on Arrhenius graphs allow melting points or phase transitions to be inferred in local domains such as membranes. With use of this technique, mitochondria from a chilling-sensit…

Saturated Fatty Acid Requirer of Neurospora crassa Open

Susan A. Henry, Alec D. Keith · 1971

Dietary saturated fatty acids containing 12- to 18-carbon atoms satisfy growth requirements of Neurospora crassa mutant cel (previously named ol ; Perkins et al., reference 11); unsaturated fatty acids are synthesized by direct desaturatio…

Membrane Structure: Spin Labeling and Freeze Etching of Mycoplasma laidlawii Open

Mark E. Tourtellotte, Daniel Branton, Alec D. Keith · 1970

A spin-labeled fatty acid was incorporated in vivo into the polar lipids of Mycoplasma laidlawii membranes. The electron paramagnetic resonance signal from either intact cells or their extracted lipids reflected the fatty acid composition …

Spin-Labeled Neurospora Mitochondria Open

Alec D. Keith, Grahame Bulfield, Wallace Snipes · 1970

Double-Bond Requirement in a Fatty Acid Desaturase Mutant of Saccharomyces cerevisiae Open

Bernadine J. Wisnieski, Alec D. Keith, Michael A. Resnick · 1970

A fatty acid Δ 9 -desaturase mutant of yeast was analyzed to establish the specificity of the fatty acid structural requirement. Several double-bond-containing and substituted fatty acids were tested. It was concluded that the presence of …

SPIN LABELS: NEW BIOLOGICAL APPLICATIONS FOR ESR Open

Wallace Snipes, Alec D. Keith · 1969

Fatty Acid Desaturase Mutants of Saccharomyces cerevisiae Open

Alec D. Keith, Michael A. Resnick, A. B. Haley · 1969

Genetic and biochemical analyses were conducted on fatty acid mutants of yeast deficient for Δ 9 -desaturase activity in the production of palmitoleate and oleate. Two genetic loci were observed and two others are inferred; three of these …

Spin-labeled mitochondrial lipids in Neurospora crassa. Open

Alec D. Keith, Alan S. Waggoner, O. Hayes Griffith · 1968

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