Christine Clayton
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eLife Assessment: The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei Open
The actin cytoskeleton is a ubiquitous feature of eukaryotic cells, yet its complexity varies across different taxa. In the parasitic protist Trypanosoma brucei, a rudimentary actomyosin system consisting of one actin gene and two myosin g…
eLife Assessment: Co-expression of the AsCas12a ultra variant, a T7 RNA Polymerase and a cytosine base editor greatly increases transfection and editing rates in Leishmania species Open
The ability to analyse the function of all genes in a genome has obvious appeal. However, this has been challenging in Leishmania due to a repetitive genome architecture, limited DNA repair mechanisms and the absence of RNA interference ma…
eLife Assessment: The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei Open
The actin cytoskeleton is a ubiquitous feature of eukaryotic cells, yet its complexity varies across different taxa. In the parasitic protist Trypanosoma brucei, a rudimentary actomyosin system consisting of one actin gene and two myosin g…
A38 EXPLORING HOW BOWEL PREPARATION CAN AFFECT INFLAMMATORY BOWEL DISEASE VIA THE GUT MICROBIOTA Open
Background Inflammatory bowel disease (IBD) is a debilitating disorder that targets the gastrointestinal (GI) tract. Although its causes remain unknown, recent studies have identified changes to the gut microbiota associated with IBD. Whil…
eLife assessment: Stumpy forms are the predominant transmissible forms of Trypanosoma brucei Open
Recently, Schuster et al. demonstrated that bloodstream slender forms of African trypanosomes are readily transmissible to young tsetse flies where they can complete their complex life cycle (). In their experimental conditions, a single s…
eLife assessment: PI(3,4,5)P3 allosteric regulation of repressor activator protein 1 controls antigenic variation in trypanosomes Open
African trypanosomes evade host immune clearance by antigenic variation, causing persistent infections in humans and animals. These parasites express a homogeneous surface coat of variant surface glycoproteins (VSGs). They transcribe one o…
Reviewer #2 (Public Review): PI(3,4,5)P3 allosteric regulation of repressor activator protein 1 controls antigenic variation in trypanosomes Open
African trypanosomes evade host immune clearance by antigenic variation, causing persistent infections in humans and animals. These parasites express a homogeneous surface coat of variant surface glycoproteins (VSGs). They transcribe one o…
eLife Assessment: Stumpy forms are the predominant transmissible forms of Trypanosoma brucei Open
Recently, Schuster et al. demonstrated that bloodstream slender forms of African trypanosomes are readily transmissible to young tsetse flies where they can complete their complex life cycle (). In their experimental conditions, a single s…
eLife Assessment: PI(3,4,5)P3 allosteric regulation of repressor activator protein 1 controls antigenic variation in trypanosomes Open
African trypanosomes evade host immune clearance by antigenic variation, causing persistent infections in humans and animals. These parasites express a homogeneous surface coat of variant surface glycoproteins (VSGs). They transcribe one o…
Reviewer #3 (Public Review): A PI(3,4,5)P3-dependent allosteric switch controls antigenic variation in trypanosomes Open
African trypanosomes evade host immune clearance by antigenic variation, causing persistent infections in humans and animals. These parasites express a homogeneous surface coat of variant surface glycoproteins (VSGs). They transcribe one o…
eLife Assessment: A PI(3,4,5)P3-dependent allosteric switch controls antigenic variation in trypanosomes Open
African trypanosomes evade host immune clearance by antigenic variation, causing persistent infections in humans and animals. These parasites express a homogeneous surface coat of variant surface glycoproteins (VSGs). They transcribe one o…
Decision letter: Gene editing and scalable functional genomic screening in Leishmania species using the CRISPR/Cas9 cytosine base editor toolbox LeishBASEedit Open
Full text Figures and data Side by side Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract CRISPR/…
Editor's evaluation: Gene editing and scalable functional genomic screening in Leishmania species using the CRISPR/Cas9 cytosine base editor toolbox LeishBASEedit Open
Full text Figures and data Side by side Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract CRISPR/…
Decision letter: Diverse evolutionary pathways challenge the use of collateral sensitivity as a strategy to suppress resistance Open
Full text Figures and data Side by side Abstract Editor's evaluation eLife digest Introduction Results Discussion Methods Appendix 1 Data availability References Decision letter Author response Article and author information Metrics Abstra…
Editor's evaluation: Diverse evolutionary pathways challenge the use of collateral sensitivity as a strategy to suppress resistance Open
Full text Figures and data Side by side Abstract Editor's evaluation eLife digest Introduction Results Discussion Methods Appendix 1 Data availability References Decision letter Author response Article and author information Metrics Abstra…
View article: Sequences and proteins that influence mRNA processing in Trypanosoma brucei: Evolutionary conservation of SR-domain and PTB protein functions
Sequences and proteins that influence mRNA processing in Trypanosoma brucei: Evolutionary conservation of SR-domain and PTB protein functions Open
Background Spliced leader trans splicing is the addition of a short, capped sequence to the 5’ end of mRNAs. It is widespread in eukaryotic evolution, but factors that influence trans splicing acceptor site choice have been little investig…
View article: Distinct mRNA and protein interactomes highlight functional differentiation of major eIF4F-like complexes from Trypanosoma brucei
Distinct mRNA and protein interactomes highlight functional differentiation of major eIF4F-like complexes from Trypanosoma brucei Open
Gene expression in pathogenic protozoans of the family Trypanosomatidae has several novel features, including multiple eIF4F-like complexes involved in protein synthesis. The eukaryotic eIF4F complex, formed mainly by eIF4E and eIF4G subun…
Roles and interactions of the specialized initiation factors <span>EIF4E2</span> , <span>EIF4E5,</span> and <span>EIF4E6</span> in <i>Trypanosoma brucei</i> : <span>EIF4E2</span> maintains the abundances of S‐phase <span>mRNAs</span> Open
Trypanosoma brucei has six versions of the cap‐binding translation initiation factor EIF4E. We investigated the functions of EIF4E2, EIF4E3, EIF4E5, and EIF4E6 in bloodstream forms. We confirmed the protein associations previously found in…