Sean C. Goetsch
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View article: Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells
Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells Open
Perturb-seq is a powerful approach to systematically assess how genes and enhancers impact the molecular and cellular pathways of development and disease. However, technical challenges have limited its application in stem-cell-based system…
View article: HAND2 invades nucleolar condensates to pioneer lineage-specific cardiac pacemaker gene programs
HAND2 invades nucleolar condensates to pioneer lineage-specific cardiac pacemaker gene programs Open
Although best known as the site for ribosome assembly, the nucleolus organizes heterochromatin into transcriptionally repressed Nucleolus-Associated Domains (NADs). NADs harbor many genes involved in cell-type specification, yet the mechan…
View article: Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells
Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells Open
Perturb-seq is a powerful approach to systematically assess how genes and enhancers impact the molecular and cellular pathways of development and disease. However, technical challenges have limited its application in stem cell-based system…
View article: Experimental procedures for TF Perturb-Seq (UT Southwestern) v2
Experimental procedures for TF Perturb-Seq (UT Southwestern) v2 Open
Optimized procedures and best practices for Perturb-Seq data production, developed at the UT Southwestern Characterization Center
View article: CHD-associated enhancers shape human cardiomyocyte lineage commitment
CHD-associated enhancers shape human cardiomyocyte lineage commitment Open
Enhancers orchestrate gene expression programs that drive multicellular development and lineage commitment. Thus, genetic variants at enhancers are thought to contribute to developmental diseases by altering cell fate commitment. However, …
View article: Global chromatin landscapes identify candidate noncoding modifiers of cardiac rhythm
Global chromatin landscapes identify candidate noncoding modifiers of cardiac rhythm Open
Comprehensive cis-regulatory landscapes are essential for accurate enhancer prediction and disease variant mapping. Although cis-regulatory element (CRE) resources exist for most tissues and organs, many rare - yet functionally important -…
View article: CHD-associated enhancers shape human cardiomyocyte lineage commitment
CHD-associated enhancers shape human cardiomyocyte lineage commitment Open
Enhancers orchestrate gene expression programs that drive multicellular development and lineage commitment. Thus, genetic variants at enhancers are thought to contribute to developmental diseases by altering cell fate commitment. However, …