Caroline Le Van Kim
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View article: Using molecular rotors to investigate hemoglobin concentration and heterogeneity in red blood cells
Using molecular rotors to investigate hemoglobin concentration and heterogeneity in red blood cells Open
We explore the potential of using the DASPI molecular rotor [trans-4-[4-(Dimethylamino)styryl]-1-methylpyridinium iodide] to probe the rheology of red blood cells. Previous investigations have demonstrated the rotor’s capacity to penetrate…
View article: Association of haemolysis markers, blood viscosity and microcirculation function with organ damage in sickle cell disease in <scp>sub‐Saharan</scp> Africa (the <scp>BIOCADRE</scp> study)
Association of haemolysis markers, blood viscosity and microcirculation function with organ damage in sickle cell disease in <span>sub‐Saharan</span> Africa (the <span>BIOCADRE</span> study) Open
Summary Sickle cell anaemia (SCA) is a monogenic disease with a highly variable clinical course. We aimed to investigate associations between microvascular function, haemolysis markers, blood viscosity and various types of SCA‐related orga…
View article: S266: DIFFERENT MOLECULAR VASO-OCCLUSIVE TRIGGERS STIMULATE DISTINCT VASO-OCCLUSIVE PROFILES: IN VIVO RESULTS FROM MICE WITH SICKLE CELL ANEMIA
S266: DIFFERENT MOLECULAR VASO-OCCLUSIVE TRIGGERS STIMULATE DISTINCT VASO-OCCLUSIVE PROFILES: IN VIVO RESULTS FROM MICE WITH SICKLE CELL ANEMIA Open
Background: Microvascular vaso-occlusion (VO) characterizes sickle cell anemia (SCA) pathophysiology and is the result of inflammatory mechanisms that promote interactions of leukocytes and erythrocytes with the endothelium, with consequen…
View article: Adenosine signaling inhibits erythropoiesis and promotes myeloid differentiation
Adenosine signaling inhibits erythropoiesis and promotes myeloid differentiation Open
Intracellular uptake of adenosine is essential for optimal erythroid commitment and differentiation of hematopoietic progenitor cells. The role of adenosine signaling is well documented in the regulation of blood flow, cell proliferation, …
View article: Relevance of <scp>Howell‐Jolly</scp> body counts for measuring spleen function in sickle cell disease
Relevance of <span>Howell‐Jolly</span> body counts for measuring spleen function in sickle cell disease Open
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View article: Lack of the human choline transporter‐like protein <scp>SLC44A2</scp> causes hearing impairment and a rare red blood phenotype
Lack of the human choline transporter‐like protein <span>SLC44A2</span> causes hearing impairment and a rare red blood phenotype Open
Blood phenotypes are defined by the presence or absence of specific blood group antigens at the red blood cell (RBC) surface, due to genetic polymorphisms among individuals. The recent development of genomic and proteomic approaches enable…
View article: Erythrocyte type 1 equilibrative nucleoside transporter expression in sickle cell disease and sickle cell trait
Erythrocyte type 1 equilibrative nucleoside transporter expression in sickle cell disease and sickle cell trait Open
Summary Hypoxia‐mediated red blood cell (RBC) sickling is central to the pathophysiology of sickle cell disease (SCD). The signalling nucleoside adenosine is thought to play a significant role in this process. This study investigated expre…
View article: Platelet caspase‐1 and Bruton tyrosine kinase activation in patients with COVID‐19 is associated with disease severity and reversed in vitro by ibrutinib
Platelet caspase‐1 and Bruton tyrosine kinase activation in patients with COVID‐19 is associated with disease severity and reversed in vitro by ibrutinib Open
Our results show that caspase-1 and BTK activation are related to disease severity and suggest the therapeutic hope raised by ibrutinib in the treatment of COVID-19 by reducing the procoagulant state of the patients.
View article: Persistence of chronic inflammation after regular blood transfusion therapy in sickle cell anemia
Persistence of chronic inflammation after regular blood transfusion therapy in sickle cell anemia Open
View article: Phagocytosis of Erythrocytes from Gaucher Patients Induces Phenotypic Modifications in Macrophages, Driving Them toward Gaucher Cells
Phagocytosis of Erythrocytes from Gaucher Patients Induces Phenotypic Modifications in Macrophages, Driving Them toward Gaucher Cells Open
Gaucher disease (GD) is caused by glucocerebrosidase deficiency leading to the accumulation of sphingolipids in macrophages named “Gaucher’s Cells”. These cells are characterized by deregulated expression of cell surface markers, abnormal …
View article: Proteomic analysis of neutrophils from patients with <scp>COVID</scp>‐19
Proteomic analysis of neutrophils from patients with <span>COVID</span>‐19 Open
International audience
View article: Lack of the human choline transporter-like protein CTL2 causes hearing impairment and a rare red blood cell phenotype
Lack of the human choline transporter-like protein CTL2 causes hearing impairment and a rare red blood cell phenotype Open
Recent genome-wide association and murine studies identified the human neutrophil antigen -3a/b polymorphism (HNA-3a/b) in SLC44A2 (rs2288904-G/A) as a risk factor in venous thromboembolism (VTE). The choline transporter-like protein CTL2 …
View article: Editorial: Inflammatory Mechanisms of Hemolytic Diseases
Editorial: Inflammatory Mechanisms of Hemolytic Diseases Open
EDITORIAL article Front. Immunol., 12 January 2022Sec. Inflammation Volume 12 - 2021 | https://doi.org/10.3389/fimmu.2021.834527
View article: Plasma microparticles of intubated COVID‐19 patients cause endothelial cell death, neutrophil adhesion and netosis, in a phosphatidylserine‐dependent manner
Plasma microparticles of intubated COVID‐19 patients cause endothelial cell death, neutrophil adhesion and netosis, in a phosphatidylserine‐dependent manner Open
Summary COVID‐19 has compelled scientists to better describe its pathophysiology to find new therapeutic approaches. While risk factors, such as older age, obesity, and diabetes mellitus, suggest a central role of endothelial cells (ECs), …
View article: Low incidence of COVID-19 severe complications in a large cohort of children with sickle cell disease: a protective role for basal interferon-1 activation?
Low incidence of COVID-19 severe complications in a large cohort of children with sickle cell disease: a protective role for basal interferon-1 activation? Open
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View article: Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected Erythrocytes
Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected Erythrocytes Open
The high prevalence of sickle cell disease in some human populations likely results from the protection afforded against severe Plasmodium falciparum malaria and death by heterozygous carriage of HbS. P. falciparum remodels the erythrocyte…
View article: Inherited glycosylphosphatidylinositol defects cause the rare Emm-negative blood phenotype and developmental disorders
Inherited glycosylphosphatidylinositol defects cause the rare Emm-negative blood phenotype and developmental disorders Open
Glycosylphosphatidylinositol (GPI) is a glycolipid that anchors >150 proteins to the cell surface. Pathogenic variants in several genes that participate in GPI biosynthesis cause inherited GPI deficiency disorders. Here, we reported tha…
View article: Deficient mitophagy pathways in sickle cell disease
Deficient mitophagy pathways in sickle cell disease Open
Summary Sickle cell disease (SCD) is characterised by chronic haemolysis and oxidative stress. Herein, we investigated 30 SCD patients and found 40% with elevated mitochondria levels (SS‐mito + ) in their mature red blood cells, while 60% …
View article: The equilibrative nucleoside transporter ENT1 is critical for nucleotide homeostasis and optimal erythropoiesis
The equilibrative nucleoside transporter ENT1 is critical for nucleotide homeostasis and optimal erythropoiesis Open
The tight regulation of intracellular nucleotides is critical for the self-renewal and lineage specification of hematopoietic stem cells (HSCs). Nucleosides are major metabolite precursors for nucleotide biosynthesis and their availability…
View article: Rapid clearance of storage-induced microerythrocytes alters transfusion recovery
Rapid clearance of storage-induced microerythrocytes alters transfusion recovery Open
Permanent availability of red blood cells (RBCs) for transfusion depends on refrigerated storage, during which morphologically altered RBCs accumulate. Among these, a subpopulation of small RBCs, comprising type III echinocytes, spheroechi…
View article: Human erythroid differentiation requires VDAC1-mediated mitochondrial clearance
Human erythroid differentiation requires VDAC1-mediated mitochondrial clearance Open
Erythroblast maturation in mammals is dependent on organelle clearance throughout terminal erythropoiesis. We studied the role of the outer mitochondrial membrane protein voltage-dependent anion channel-1 (VDAC1) in human terminal erythrop…
View article: Metabolic rejuvenation upgrades circulatory functions of red blood cells stored under blood bank conditions
Metabolic rejuvenation upgrades circulatory functions of red blood cells stored under blood bank conditions Open
Background Red blood cells (RBC) change upon hypothermic conservation, and storage for 6 weeks is associated with the short‐term clearance of 15% to 20% of transfused RBCs. Metabolic rejuvenation applied to RBCs before transfusion replenis…
View article: Cell‐derived microparticles and sickle cell disease chronic vasculopathy in sub‐Saharan Africa: A multinational study
Cell‐derived microparticles and sickle cell disease chronic vasculopathy in sub‐Saharan Africa: A multinational study Open
Summary Although most individuals with sickle cell disease (SCD) live in sub‐Saharan Africa, the natural history of the disease on this continent remains largely unknown. Intravascular haemolysis results in activation of circulating blood …
View article: Downregulation of Mitochondrial TSPO Inhibits Mitophagy and Reduces Enucleation During Human Terminal Erythropoiesis
Downregulation of Mitochondrial TSPO Inhibits Mitophagy and Reduces Enucleation During Human Terminal Erythropoiesis Open
Translocator protein (TSPO) and voltage dependent anion channels (VDAC) are two proteins forming a macromolecular complex in the outer mitochondrial membrane that is involved in pleiotropic functions. Specifically, these proteins were desc…
View article: Oxidative stress activates red cell adhesion to laminin in sickle cell disease
Oxidative stress activates red cell adhesion to laminin in sickle cell disease Open
Vaso-occlusive crises are the hallmark of sickle cell disease (SCD). They are believed to occur in two steps, starting with adhesion of deformable low-dense red blood cells (RBCs), or other blood cells such as neutrophils, to the wall of p…
View article: Effects of sphingolipids overload on red blood cell properties in Gaucher disease
Effects of sphingolipids overload on red blood cell properties in Gaucher disease Open
Gaucher disease (GD) is a genetic disease with mutations in the GBA gene that encodes glucocerebrosidase causing complications such as anaemia and bone disease. GD is characterized by accumulation of the sphingolipids (SL) glucosylceramide…
View article: The proteome of neutrophils in sickle cell disease reveals an unexpected activation of interferon alpha signaling pathway
The proteome of neutrophils in sickle cell disease reveals an unexpected activation of interferon alpha signaling pathway Open
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View article: Semaphorin 7A: A novel marker of disease activity in Gaucher disease
Semaphorin 7A: A novel marker of disease activity in Gaucher disease Open
Gaucher disease (GD) is a recessively inherited lysosomal storage disorder in which sphingolipids accumulates in the macrophages that transform into Gaucher cells. A growing body of evidence indicates that red blood cells (RBCs) represent …
View article: Lack of the multidrug transporter MRP4/ABCC4 defines the PEL-negative blood group and impairs platelet aggregation
Lack of the multidrug transporter MRP4/ABCC4 defines the PEL-negative blood group and impairs platelet aggregation Open
The rare PEL-negative phenotype is one of the last blood groups with an unknown genetic basis. By combining whole-exome sequencing and comparative global proteomic investigations, we found a large deletion in the ABCC4/MRP4 gene encoding a…
View article: Dimerization and phosphorylation of Lutheran/basal cell adhesion molecule are critical for its function in cell migration on laminin
Dimerization and phosphorylation of Lutheran/basal cell adhesion molecule are critical for its function in cell migration on laminin Open