Jean‐Christophe Leroux
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View article: A Degradable Device for Sustainable Capillary Blood Sampling
A Degradable Device for Sustainable Capillary Blood Sampling Open
Capillary blood sampling plays a crucial role in diagnostic decentralization, yet most microsampling devices remain expensive, limiting their use mainly to developed countries. To improve accessibility, a cost‐effective silicone device cap…
View article: Isolation of bacterial extracellular vesicles from raw samples using a portable microstructured electrochemical device
Isolation of bacterial extracellular vesicles from raw samples using a portable microstructured electrochemical device Open
Bacterial extracellular vesicles (EVs) are nanosized vesicles released by both Gram-negative and Gram-positive bacteria, playing critical roles in microbial communication, host-pathogen interactions, and immune modulation. Despite their si…
View article: The intracellular journey of DNA delivered with the protein-based transfection system TFAMoplex and Lipofectamine
The intracellular journey of DNA delivered with the protein-based transfection system TFAMoplex and Lipofectamine Open
In non-viral DNA delivery, assessing the cellular trafficking of DNA is crucial to understand how transfection systems interact with the cellular machinery. Using a combination of innovative and established imaging assays and flow cytometr…
View article: Rapid selection of transgenic mammalian cells via diphtheria toxin resistance
Rapid selection of transgenic mammalian cells via diphtheria toxin resistance Open
The ability to generate stable transgenic mammalian cell lines is crucial to the investigation of gene functions and the production of recombinant proteins. Mammalian cells can be readily transfected in cell culture settings via both viral…
View article: Encapsulation of protein/DNA complexes into unilamellar liposomes via annexin-mediated membrane recruitment and sonication
Encapsulation of protein/DNA complexes into unilamellar liposomes via annexin-mediated membrane recruitment and sonication Open
This paper reports an effective protocol to encapsulate native protein/DNA complexes into unilamellar vesicles composed of natural lipids without the use of organic solvents, in physiological buffers, and at low protein/DNA concentrations.…
View article: VWA7 – A Putative Human Phosphatidylcholine-specific Phospholipase C
VWA7 – A Putative Human Phosphatidylcholine-specific Phospholipase C Open
For over four decades, the existence of mammalian phosphatidylcholine-specific phospholipase C (pc-PLC) has been reported. However, the gene encoding this enzyme has not been unambiguously determined to date. This study presents human von …
View article: Hurdles to healing: Overcoming cellular barriers for viral and nonviral gene therapy
Hurdles to healing: Overcoming cellular barriers for viral and nonviral gene therapy Open
Gene delivery offers great potential for treating various diseases, yet its success requires overcoming several biological barriers. These hurdles span from extracellular degradation, reaching the target cells, and inefficient cellular upt…
View article: Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery
Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery Open
Gene delivery systems must overcome multiple barriers, with endosomal escape representing a prominent obstacle. We have previously shown that a bacterial phospholipase C (PLC) enabled endosomal escape of a non‐viral protein‐based DNA deliv…
View article: Optimized suction patch design for enhanced transbuccal macromolecular drug delivery
Optimized suction patch design for enhanced transbuccal macromolecular drug delivery Open
Peptides represent a rapidly expanding class of drugs with broad therapeutic potential. However, due to their large molecular weight and susceptibility to degradation in the gastrointestinal tract, most peptide drugs are administered via s…
View article: Loading of Extracellular Vesicles with Nucleic Acids via Hybridization with Non‐Lamellar Liquid Crystalline Lipid Nanoparticles
Loading of Extracellular Vesicles with Nucleic Acids via Hybridization with Non‐Lamellar Liquid Crystalline Lipid Nanoparticles Open
The translation of cell‐derived extracellular vesicles (EVs) into biogenic gene delivery systems is limited by relatively inefficient loading strategies. In this work, the loading of various nucleic acids into small EVs via their spontaneo…
View article: 3D printed drug-eluting stents: Toward personalized therapy for airway stenosis
3D printed drug-eluting stents: Toward personalized therapy for airway stenosis Open
Airway stents are life-saving medical devices used to treat malignant or benign central airway stenoses. Unfortunately, these devices, typically manufactured from silicone alone and/or nitinol, can result in serious complications, such as …
View article: Extracellular vesicles versus lipid nanoparticles for the delivery of nucleic acids
Extracellular vesicles versus lipid nanoparticles for the delivery of nucleic acids Open
Extracellular vesicles (EVs) are increasingly investigated for delivering nucleic acid (NA) therapeutics, leveraging their natural role in transporting NA and protein-based cargo in cell-to-cell signaling. Their synthetic counterparts, lip…
View article: Rapid selection of transgenic mammalian cells via diphtheria toxin resistance
Rapid selection of transgenic mammalian cells via diphtheria toxin resistance Open
The ability to generate stable transgenic mammalian cell lines is crucial to the investigation of gene functions and the production of recombinant proteins. Currently, mammalian cells can be readily transfected in cell culture settings via…
View article: Lighting up aggregate emission of perylene diimide by leveraging polymerization-mediated through-space charge transfer and π-π stacking
Lighting up aggregate emission of perylene diimide by leveraging polymerization-mediated through-space charge transfer and π-π stacking Open
The molecular engineering of fluorescent organic/polymeric materials, specifically those emitting in the deep red to near-infrared spectrum, is vital for advancements in optoelectronics and biomedicine. Perylene diimide (PDI), a well-known…
View article: Effects of shape and structure of a new 3D-printed personalized bioresorbable tracheal stent on fit and biocompatibility in a rabbit model
Effects of shape and structure of a new 3D-printed personalized bioresorbable tracheal stent on fit and biocompatibility in a rabbit model Open
To date, several types of airway stents are available to treat central airway obstructions. However, the ideal stent that can overcome anatomical, mechanical and microbiological issues is still awaited. In addition, therapeutic effect and …
View article: Gastrointestinal Permeation Enhancers Beyond Sodium Caprate and SNAC ‐ What is Coming Next?
Gastrointestinal Permeation Enhancers Beyond Sodium Caprate and SNAC ‐ What is Coming Next? Open
Oral peptide delivery is trending again. Among the possible reasons are the recent approvals of two oral peptide formulations, which represent a huge stride in the field. For the first time, gastrointestinal (GI) permeation enhancers (PEs)…
View article: Revival of Bioengineered Proteins as Carriers for Nucleic Acids
Revival of Bioengineered Proteins as Carriers for Nucleic Acids Open
ADVERTISEMENT RETURN TO ARTICLES ASAPViewpointNEXTRevival of Bioengineered Proteins as Carriers for Nucleic AcidsDavid SchererDavid SchererInstitute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Z…
View article: Loading of extracellular vesicles with nucleic acids via hybridization with sponge-like lipid nanoparticles
Loading of extracellular vesicles with nucleic acids via hybridization with sponge-like lipid nanoparticles Open
The translation of cell-derived extracellular vesicles (EVs) into biogenic gene delivery systems is limited by relatively inefficient loading strategies. In this work, we describe the loading of various nucleic acids into small EVs via the…
View article: Transport of CLCA2 to the nucleus by extracellular vesicles controls keratinocyte survival and migration
Transport of CLCA2 to the nucleus by extracellular vesicles controls keratinocyte survival and migration Open
Chloride channel accessory 2 (CLCA2) is a transmembrane protein, which promotes adhesion of keratinocytes and their survival in response to hyperosmotic stress. Here we show that CLCA2 is transported to the nucleus of keratinocytes via ext…
View article: Loss of <i>Dnmt3a</i> increases self-renewal and resistance to pegIFN-α in <i>JAK2</i>-V617F–positive myeloproliferative neoplasms
Loss of <i>Dnmt3a</i> increases self-renewal and resistance to pegIFN-α in <i>JAK2</i>-V617F–positive myeloproliferative neoplasms Open
Pegylated interferon alfa (pegIFN-α) can induce molecular remissions in patients with JAK2-V617F–positive myeloproliferative neoplasms (MPNs) by targeting long-term hematopoietic stem cells (LT-HSCs). Additional somatic mutations in genes …
View article: A Bioinspired and Cost‐Effective Device for Minimally Invasive Blood Sampling
A Bioinspired and Cost‐Effective Device for Minimally Invasive Blood Sampling Open
Conventional venipuncture is invasive and challenging in low and middle‐income countries. Conversely, point‐of‐care devices paired with fingersticks, although less invasive, suffer from high variability and low blood volume collection. Rec…
View article: Colonic Delivery of Aqueous Suspensions Using 3D Printed Capsules
Colonic Delivery of Aqueous Suspensions Using 3D Printed Capsules Open
The efficient oral delivery of aqueous suspensions to the distal intestine or the colon is a significant pharmaceutical challenge because few capsules are sufficiently stable to protect aqueous suspensions while enduring the conditions dur…
View article: Strong Aggregate Emission of Perylene Diimide in Solid State Enabled by Polymerization-Mediated Charge Transfer
Strong Aggregate Emission of Perylene Diimide in Solid State Enabled by Polymerization-Mediated Charge Transfer Open
The molecular engineering of fluorescent organic/polymeric materials, specifically those emitting in the deep red to near-infrared spectrum, is vital for advancements in optoelectronics and biomedicine. Perylene-3,4,9,10-tetracarboxylic ac…