Ciara K. O’Sullivan
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View article: Quantum-enhanced nanodiamond rapid test advances early SARS-CoV-2 antigen detection in clinical diagnostics
Quantum-enhanced nanodiamond rapid test advances early SARS-CoV-2 antigen detection in clinical diagnostics Open
Quantum biosensors, which harness quantum effects to detect biomarkers, could address the urgent need for more sensitive rapid diagnostics. Lateral flow tests using nitrogen-vacancy centres in nanodiamond labels offer high sensitivity and …
View article: Evaluation of Cardiovascular Risk Factor Control Among People with Diabetes in the Community Pharmacy Setting—A Descriptive Observational Study
Evaluation of Cardiovascular Risk Factor Control Among People with Diabetes in the Community Pharmacy Setting—A Descriptive Observational Study Open
Background: In some countries, community pharmacists provide advanced services to people with diabetes that improve glycaemic control and cardiovascular risk. This study aims to describe the cardiovascular risk profile of people with diabe…
View article: Versatile Enzymatic Approach for Truncation, Labeling, and Mutation of Nucleobase‐Modified Aptamers Demonstrated on Indole‐Modified <i>β</i> ‐Conglutin Aptamer
Versatile Enzymatic Approach for Truncation, Labeling, and Mutation of Nucleobase‐Modified Aptamers Demonstrated on Indole‐Modified <i>β</i> ‐Conglutin Aptamer Open
A new enzymatic method for truncation of aptamers from 5’‐end has been developed and demonstrated on a newly selected indole‐modified β ‐conglutin aptamer. This method relies on extension of a primer containing ribonucleotides, which can b…
View article: Development of a Protein-Free Nucleic Acid Lateral Flow Assay for <i>Trichomonas vaginalis</i>
Development of a Protein-Free Nucleic Acid Lateral Flow Assay for <i>Trichomonas vaginalis</i> Open
In line with the global research priorities on sexually transmitted infections (STIs), we developed a molecular point-of-care test (POCT) for the parasite Trichomonas vaginalis that causes the STI trichomoniasis. Trichomoniasis rema…
View article: Quantum-enhanced nanodiamond rapid test advances early SARS-CoV-2 antigen detection in clinical diagnostics
Quantum-enhanced nanodiamond rapid test advances early SARS-CoV-2 antigen detection in clinical diagnostics Open
Worldwide, the urgent need for more sensitive rapid lateral flow tests (LFTs) for early disease diagnosis is driving advances in quantum technologies. Spin-enhanced nanodiamond LFTs offer the potential for a sensitivity step change, yet to…
View article: Aptamer lateral flow assay for the rapid detection of histamine in fish and human blood
Aptamer lateral flow assay for the rapid detection of histamine in fish and human blood Open
The ingestion of histamine-rich foods or deficiencies in histamine-degrading enzymes can lead to histamine accumulation and produce intolerance, mimicking an allergic reaction with symptoms ranging from mild to even severe and potentially …
View article: Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish
Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish Open
View article: Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of <i>Trichomonas vaginalis</i>
Biotin/avidin-free sandwich aptamer-based lateral flow assay (ALFA) for the diagnosis of <i>Trichomonas vaginalis</i> Open
Aptamer lateral flow assay (ALFA) for testing T. vaginalis (Tv) in cervicovaginal lavage (CVL).
View article: Sandwich enzyme-linked aptamer-based assay for the detection of Trichomonas vaginalis
Sandwich enzyme-linked aptamer-based assay for the detection of Trichomonas vaginalis Open
Trichomoniasis is the most prevalent curable, non-viral sexually transmitted infection (STI), with an estimated 156 million new infections in 2020. It can potentially result in adverse birth outcomes as well as infertility in men, whilst i…
View article: Immunoglobulins in COVID-19 pneumonia: from the acute phase to the recovery phase
Immunoglobulins in COVID-19 pneumonia: from the acute phase to the recovery phase Open
View article: Semiautomated Electrochemical Melting Curve Analysis Device for the Detection of an Osteoporosis Associated Single Nucleotide Polymorphism in Blood
Semiautomated Electrochemical Melting Curve Analysis Device for the Detection of an Osteoporosis Associated Single Nucleotide Polymorphism in Blood Open
The detection of single nucleotide polymorphisms (SNPs) is of increasing importance in many areas including clinical diagnostics, patient stratification for pharmacogenomics, and advanced forensic analysis. In the work reported, we apply a…
View article: Normalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition
Normalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition Open
The previously reported approach of orthogonal multipotential redox coding of all four DNA bases allowed only analysis of the relative nucleotide composition of short DNA stretches. Here, we present two methods for normalization of the ele…
View article: Generic Platform for the Multiplexed Targeted Electrochemical Detection of Osteoporosis-Associated Single Nucleotide Polymorphisms Using Recombinase Polymerase Solid-Phase Primer Elongation and Ferrocene-Modified Nucleoside Triphosphates
Generic Platform for the Multiplexed Targeted Electrochemical Detection of Osteoporosis-Associated Single Nucleotide Polymorphisms Using Recombinase Polymerase Solid-Phase Primer Elongation and Ferrocene-Modified Nucleoside Triphosphates Open
Osteoporosis is a multifactorial disease influenced by genetic and environmental factors, which contributes to an increased risk of bone fracture, but early diagnosis of this disease cannot be achieved using current techniques. We describe…
View article: 470 Evaluation of cardiovascular risk factor control among people with diabetes in the community pharmacy setting – a cross sectional study
470 Evaluation of cardiovascular risk factor control among people with diabetes in the community pharmacy setting – a cross sectional study Open
Introduction Diabetes affects almost 140,000 Irish people and the prevalence is increasing.(1) In Ireland there is no structured programme in place for community pharmacists to provide care to people with diabetes, outside of dispensing me…
View article: Selection of G-rich ssDNA aptamers for the detection of enterotoxins of the cholera toxin family
Selection of G-rich ssDNA aptamers for the detection of enterotoxins of the cholera toxin family Open
Cholera and milder diarrheal disease are caused by Vibrio cholerae and enterotoxigenic Escherichia coli and are still a prominent public health concern. Evaluation of suspicious isolates is essential for the rapid containment of acute diar…
View article: Ultrasensitive determination of β-conglutin food allergen by means an aptamer assay based on inductively coupled plasma mass spectrometry detection
Ultrasensitive determination of β-conglutin food allergen by means an aptamer assay based on inductively coupled plasma mass spectrometry detection Open
View article: A Label-Free Gold Nanoparticles-Based Optical Aptasensor for the Detection of Retinol Binding Protein 4
A Label-Free Gold Nanoparticles-Based Optical Aptasensor for the Detection of Retinol Binding Protein 4 Open
Retinol-binding protein 4 (RBP4) has been implicated in insulin resistance in rodents and humans with obesity and T2DM, making it a potential biomarker for the early diagnosis of T2DM. However, diagnostic tools for low-level detection of R…
View article: Exploiting the Nucleic Acid Nature of Aptamers for Signal Amplification
Exploiting the Nucleic Acid Nature of Aptamers for Signal Amplification Open
Aptamer-based assays and sensors are garnering increasing interest as alternatives to antibodies, particularly due to their increased flexibility for implementation in alternative assay formats, as they can be employed in assays designed f…
View article: Novel nandrolone aptamer for rapid colorimetric detection of anabolic steroids
Novel nandrolone aptamer for rapid colorimetric detection of anabolic steroids Open
The illicit use of anabolic androgenic steroids (AAS) as performance-enhancing drugs remains a global issue threatening not only the credibility of competitive sports but also public health due to the well-documented adverse effects they e…
View article: Patterns and mechanisms of major trauma injuries during and after the UK Covid-19 Nationwide lockdown: analysis from a UK Major Trauma Centre
Patterns and mechanisms of major trauma injuries during and after the UK Covid-19 Nationwide lockdown: analysis from a UK Major Trauma Centre Open
Purpose To compare patterns and mechanisms of injuries during and after the UK Nationwide lockdown during the COVID-19 pandemic. Methods This prospective cohort study included all major trauma admissions during the 10-week period of the na…
View article: Recall response to COVID-19 antigen is preserved in people with multiple sclerosis on anti-CD20 medications – A pilot study
Recall response to COVID-19 antigen is preserved in people with multiple sclerosis on anti-CD20 medications – A pilot study Open
View article: Long-term immunological consequences of anti-CD20 therapies on humoral responses to COVID-19 vaccines in multiple sclerosis: an observational study
Long-term immunological consequences of anti-CD20 therapies on humoral responses to COVID-19 vaccines in multiple sclerosis: an observational study Open
Background: Anti-CD20 therapies induce pronounced B-cell depletion and blunt humoral responses to vaccines. Recovery kinetics of anti-CD20 therapy-mediated cellular and humoral effects in people with multiple sclerosis (pwMS) are poorly de…
View article: Aptamer Sandwich Assay for the Detection of SARS-CoV-2 Spike Protein Antigen
Aptamer Sandwich Assay for the Detection of SARS-CoV-2 Spike Protein Antigen Open
The novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) emerged at the end of 2019, resulting in the ongoing COVID-19 pandemic. The high transmissibility of the virus and the substantial number of asymptomatic individuals have…
View article: Low-Cost Platform for Multiplexed Electrochemical Melting Curve Analysis
Low-Cost Platform for Multiplexed Electrochemical Melting Curve Analysis Open
Detection and identification of single nucleotide polymorphisms (SNPs) have garnered increasing interest in the past decade, finding potential application in detection of antibiotic resistance, advanced forensic science, as well as clinica…
View article: Solid-phase recombinase polymerase amplification using ferrocene-labelled dNTPs for electrochemical detection of single nucleotide polymorphisms
Solid-phase recombinase polymerase amplification using ferrocene-labelled dNTPs for electrochemical detection of single nucleotide polymorphisms Open
Hypertrophic cardiomyopathies (HCM) are the principal cause of sudden cardiac death in young athletes and it is estimated that 1 in 500 people have HCM. The aim of this work was to develop an electrochemical platform for the detection of H…
View article: Electrochemical Detection of Single-Nucleotide Polymorphism Associated with Rifampicin Resistance in <i>Mycobacterium tuberculosis</i> Using Solid-Phase Primer Elongation with Ferrocene-Linked Redox-Labeled Nucleotides
Electrochemical Detection of Single-Nucleotide Polymorphism Associated with Rifampicin Resistance in <i>Mycobacterium tuberculosis</i> Using Solid-Phase Primer Elongation with Ferrocene-Linked Redox-Labeled Nucleotides Open
Here, we report the electrochemical detection of single-point mutations using solid-phase isothermal primer elongation with redox-labeled oligonucleotides. A single-base mutation associated with resistance to rifampicin, an antibiotic comm…
View article: Pre-Existing Humoral Immunological Memory Is Retained in Patients with Multiple Sclerosis Receiving Cladribine Therapy
Pre-Existing Humoral Immunological Memory Is Retained in Patients with Multiple Sclerosis Receiving Cladribine Therapy Open
Cladribine (CLAD) is a lymphodepleting agent approved for active relapsing multiple sclerosis (MS). The impact of CLAD on the adaptive humoral immune system has not sufficiently been studied. This study aimed to assess the influence of CLA…
View article: Elevated Anti-SARS-CoV-2 Antibodies and IL-6, IL-8, MIP-1β, Early Predictors of Severe COVID-19
Elevated Anti-SARS-CoV-2 Antibodies and IL-6, IL-8, MIP-1β, Early Predictors of Severe COVID-19 Open
Viral and host immune kinetics during acute COVID-19 and after remission of acute symptoms need better characterization. SARS-CoV-2 RNA, anti-SARS-CoV-2 IgA, IgM, and IgG antibodies, and proinflammatory cytokines were measured in sequentia…
View article: Solid-Phase Primer Elongation Using Biotinylated dNTPs for the Detection of a Single Nucleotide Polymorphism from a Fingerprick Blood Sample
Solid-Phase Primer Elongation Using Biotinylated dNTPs for the Detection of a Single Nucleotide Polymorphism from a Fingerprick Blood Sample Open
Isothermal recombinase polymerase amplification-based solid-phase primer extension is used for the optical detection of a hypertrophic cardiomyopathy associated single nucleotide polymorphism (SNP) in a fingerprick blood sample. The assay …
View article: Hybrid Antibody–Aptamer Assay for Detection of Tetrodotoxin in Pufferfish
Hybrid Antibody–Aptamer Assay for Detection of Tetrodotoxin in Pufferfish Open
The marine toxin tetrodotoxin (TTX) poses a great risk to public health safety due to its severe paralytic effects after ingestion. Seafood poisoning caused by the consumption of contaminated marine species like pufferfish due to its expan…