José Geraldo da Cruz Pradella

Cost-effective production process of scFv antibody fragments against Shiga toxin 2 via recombinant E. coli Open

Marcela Rodrigues Moreira Guimarães, Daniela Luz, Elisabeth de Fátima Pires Augusto, Lúcia Vieira, Maricília Silva Costa , et al. · 2025

Shiga toxin (Stx)-producing Escherichia coli (STEC) and its subgroup enterohemorrhagic E. coli are significant pathogens responsible for diarrhea, which can progress to hemorrhagic colitis and hemolytic uremic syndrome (HUS), the leading c…

Inhibition of Development and Metabolism of Dual-Species Biofilms of Candida albicans and Candida krusei (Pichia kudriavzevii) by Organoselenium Compounds Open

Gabriela de Souza Calvi, Giulia Nicolle Jácome Cartaxo, Qiuxin Lin Carretoni, André Luiz Missio da Silva, Denilson Nogueira de Moraes , et al. · 2024

Although Candida albicans is the most frequently identified Candida species in clinical settings, a significant number of infections related to the non-albicans Candida (NAC) species, Candida krusei, has been reported. Both species are abl…

Cellulase and Xylanase Enzymes from Trichoderma reesei RUT-C30 Using Pretreated Sugarcane Bagasse in a Biorefinery Environment Open

Diogo Robl, Beatriz Merchel Piovezan Pereira, Aline Carvalho da Costa, José Geraldo da Cruz Pradella · 2024

Biofuels and other bioproducts derived from lignocellulosic feedstocks represent environmentally sustainable pathways that have the potential to compete favorably with conventional petrochemical counterparts. Realizing their economic viabi…

Leachate landfill treatment using plasma and Ozone Open

Anderson Carlos Faria, Leticia F. Sousa, Ângela Aparecida Vieira, José Geraldo da Cruz Pradella, Lúcia Vieira · 2023

Plasma discharge in liquid was used, and a combination system with plasma and ozone was tested looking for an effective reduction of organic material, cell growth, and discoloring process of leachate landfill. The treatments were divided i…

OTIMIZAÇÃO DA HIDRÓLISE DO BAGAÇO DE CANA PRÉ-TRATADO PARA USO COMO FONTE DE CARBONO Open

Juçara Cristina Pereira Sousa, Rafaela Couto, José Geraldo da Cruz Pradella · 2023

CARATERÍSTICAS DE CRESCIMENTO DE RHODOSPORIDIUM THORULOPSIS CULTIVADAS EM BIORREATOR Open

Laís Sonnewend, Maria Antonia de Mello e Albuquerque Barboza, Thainá Barbosa de Lima Cordeiro, Rafaela Couto, Juçara Cristina Pereira Sousa , et al. · 2023

An insight into cellulolytic capacity of the <i>Trichoderma harzianum</i> P49P11 revealed by omics approaches Open

Carla Aloia Codima, Geizecler Tomazetto, Gabriela Félix Persinoti, Diego Mauricio Riaño‐Pachón, Fábio M. Squina , et al. · 2022

Cellulases are a group of enzymes with several applications in biofuel production, and the paper, food, pharmaceutical, and chemical industries. Trichoderma harzianum P49P11 secrete all cellulases with high efficiency, representing an alte…

Oxidative cleavage of polysaccharides by a termite-derived <i>superoxide dismutase</i> boosts the degradation of biomass by glycoside hydrolases Open

João Paulo L. Franco Cairo, F Mandelli, Robson Tramontina, David Cannella, Alessandro Paradisi , et al. · 2022

A Cu/Zn superoxide dismutase isolated from termite gut can oxidise glucose polymers, boosting the activity of glycoside hydrolases.

Unveiling Xylanolytic Enzymes Production of Talaromyces wortmannii DR49 on Industrial Agro Wastes Open

Diogo Robl, Vânia Aparecida Vicente, Ida Chapaval Pimentel, Gabriel Padilla, José Geraldo da Cruz Pradella · 2021

Xylan degradation is an important step in different industries, such as in biorefinery for biomass hydrolysis. Talaromyces wortmannii is a known fungus due to second metabolite production but only few works showed the xylanolytic potential…

A possible influence of extracellular polysaccharides on the analysis of intracellular metabolites from Trichoderma harzianum grown under carbon-limited conditions Open

Lucas Gelain, José Geraldo da Cruz Pradella, Aline Carvalho da Costa, Luuk A. M. van der Wielen, Walter M. van Gulik · 2020

Continuous production of enzymes under carbon-limited conditions by Trichoderma harzianum P49P11 Open

Lucas Gelain, Esther Kingma, José Geraldo da Cruz Pradella, Aline Carvalho da Costa, Luuk A. M. van der Wielen , et al. · 2020

Carbon-limited chemostat cultures were performed using different carbon sources (glucose, 10 and 20 g/L; sucrose, 10 g/L; fructose/glucose, 5.26/5.26 g/L; carboxymethyl cellulose, 10 g/L; and carboxymethyl cellulose/glucose, 5/5 g/L) to ve…

Mathematical modelling for the optimization of cellulase production using glycerol for cell growth and cellulose as the inducer substrate Open

Lucas Gelain, Luuk A. M. van der Wielen, Walter M. van Gulik, José Geraldo da Cruz Pradella, Aline Carvalho da Costa · 2020

Cellulase production can be divided into two steps: growth stage; followed by an induction stage. To develop a mathematical model for the optimization of this strategy, two sets of experiments were performed in batch mode for parameter est…

Induction of fungal cellulolytic enzymes using sugarcane bagasse and xylose-rich liquor as substrates Open

Sidnei Emílio Bordignon, Priscila da Silva Delabona, Deise Juliana da Silva Lima, Olavo Micali Perrone, Márcia Gleice da Silva Souza , et al. · 2020

Analysis of the proteins secreted by Trichoderma harzianum P49P11 under carbon-limited conditions Open

Lucas Gelain, Martin Pabst, José Geraldo da Cruz Pradella, Aline Carvalho da Costa, Luuk A. M. van der Wielen , et al. · 2020

Plasma in-Liquid Using Non-contact Electrodes: A Method of Pretreatment to Enhance the Enzymatic Hydrolysis of Biomass Open

F. Miranda, Sarita Cândida Rabelo, José Geraldo da Cruz Pradella, C. Di Carli, Gilberto Petraconi Filho , et al. · 2019

Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production Open

Bruna Soares Fernandes, Óscar Dias, Gisela Lara da Costa, Antonio A. Kaupert Neto, Tiago Resende , et al. · 2019

A NOVEL Scytalidium SPECIES: UNDERSTAND THE CELLULOLYTIC SYSTEM FOR BIOMASS SACCHARIFICATION Open

Priscila da Silva Delabona, Mateus R. Silva, Maria Paula Paixão, Deise Juliana da Silva Lima, Gisele N. Rodrigues , et al. · 2019

In order to overcome the bottlenecks related to lignocellulosic-derived sugars, the search for more efficient enzymatic cocktails, containing a broad-spectrum of specific activities, relies on an important feature. This paper describes new…

Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation Open

Diogo Robl, Carla Montanari Mergel, Patrícia dos Santos Costa, José Geraldo da Cruz Pradella, Gabriel Padilla · 2019

Tailor made enzymatic preparation must be design to hydrolyze efficiently plant biomass, once that each plant biomass possesses a distinct cell wall composition. Most of actinomycetes used for plant cell wall degradation are focused on the…

Data of added-value lipid production, Arachidonic acid, among other lipids by Mortierella elongata, using low cost simulated wastewater Open

Bruna Soares Fernandes, Márcia Cristina Fernandes Messias, Patrícia de Oliveira Carvalho, Marcelo Zaiat, José Geraldo da Cruz Pradella · 2017

Eicosapentaenoic acid (EPA) production in silico by Pythium irregulare, as value-added product, using sugarcane vinasse as carbon source Open

Bruna Soares Fernandes, Óscar Dias, Marcelo Zaiat, José Geraldo da Cruz Pradella, Igor Tenório Marinho da Rocha · 2017

High value added lipids produced by microorganisms: a potential use of sugarcane vinasse Open

Bruna Soares Fernandes, João Paulo Fernandes Vieira, Fabiano Jares Contesini, Paulo E. Mantelatto, Marcelo Zaiat , et al. · 2017

This review aims to present an innovative concept of high value added lipids produced by heterotrophic microorganisms, bacteria and fungi, using carbon sources, such as sugars, acids and alcohols that could come from sugarcane vinasse, whi…

Potassium biphthalate buffer for pH control to optimize glycosyl hydrolase production in shake flasks using filamentous fungi Open

Patrícia dos Santos Costa, Diogo Robl, Isabelle Cristine da Costa, Deise Juliana da Silva Lima, Aline Carvalho da Costa , et al. · 2017

The optimization of culture medium with statistical methods is widely used in filamentous fungi glycosyl hydrolase production. The implementation of such methodology in bioreactors is very expensive as it requires several pH-controlled sys…

Myceliophthora thermophila M77 utilizes hydrolytic and oxidative mechanisms to deconstruct biomass Open

H. Brognaro, Thaís Milena de Souza Bezerra, José Geraldo da Cruz Pradella, Priscila da Silva Delabona, Deise Juliana da Silva Lima , et al. · 2016

Use of Ascomycete Extracts in Enzymatic Cocktail Formulations Increases Sugar Cane Bagasse Hydrolysis Open

Diogo Robl, Patrícia dos Santos Costa, Sarita Cândida Rabelo, Priscila da Silva Delabona, Deise Juliana da Silva Lima , et al. · 2016

INFLUÊNCIA DO CONTROLE DO PH NA MODULAÇÃO E OTIMIZAÇÃO DO MEIO DE CULTURA PARA A PRODUÇÃO DE GLICOHIDROLASES POR FUNGOS FILAMENTOSOS Open

P. S. Costa, Diogo Robl, David Kevim dos Santos Lima, José Geraldo da Cruz Pradella · 2015

O melhoramento de meio de cultura por metodologias estatísticas é de grande interesse para a confecção de meios ótimos de produção das glicohidrolases, por fungos filamentosos. A execução de tais metodologias em reatores de bancadas com pH…

MODELAGEM MATEMÁTICA DA PRODUÇÃO DE ENZIMAS UTILIZANDO Trichoderma harzianum EM MEIO SUBMERSO E BAGAÇO DE CANA-DE-AÇÚCAR COMO FONTE DE CARBONO Open

Lucas Gelain, Aline Carvalho da Costa, José Geraldo da Cruz Pradella · 2015

A hidrólise enzimática de materiais lignocelulósicos é uma etapa importante na produção de etanol de segunda geração. As enzimas responsáveis pela hidrólise podem ser obtidas a partir de cultivos submersos utilizando fungos filamentosos co…

PRODUÇÃO DE ENZIMAS ACESSÓRIAS RECOMBINANTES PARA SUPLEMENTAÇÃO DE COQUETEL ENZIMÁTICO DE L.THEOBROMAE PARA APLICAÇÃO NA HIDRÓLISE DO BAGAÇO Open

Kinya Katayama, A. G. TAVARES, Cassiano Batesttin Costa, Viviane Maimoni Gonçalves, José Geraldo da Cruz Pradella , et al. · 2015

A produção de coquetéis enzimáticos eficientes é imprescindível para a hidrólise de material lignocelulósico visando à obtenção de açúcares fermentescíveis. Uma das abordagens mais promissoras é o enriquecimento de coquetéis fúngicos em te…

EVOLUÇÃO DA LINHAGEM TRICHODERMA HARZIANUM P49P11 SELVAGEM QUANTO À PRODUÇÃO DE GLICOHIDROLÁSES POR TRATAMENTO MUTAGÊNICO PELO ETIL METANO SULFONATO (EMS) SEGUIDA DE SELEÇÃO POR VELOCIDADE DE FORMAÇÃO DE HALO EM AGAR-CELULOSE Open

P. S. COSTA, Isabelle Cristine da Costa, Aline Carvalho da Costa, P. S. Delabona, José Geraldo da Cruz Pradella · 2015

As utilizações de técnicas de mutagênese clássica para fungos filamentosos, desde muitos anos, vêm sendo uma estratégia bastante atrativa para incrementar a produção de glicohidrolases. Esse trabalho teve como objetivo a realização da evol…

ESTUDO DA ADSORÇÃO DE CELULASE EM BAGAÇO DE CANA-DE-AÇÚCAR PRÉ-TRATADO DURANTE HIDRÓLISE ENZIMÁTICA Open

João Moreira Neto, Daniele Longo Machado, Antonio Bonomi, Sarita Cândida Rabelo, José Geraldo da Cruz Pradella , et al. · 2015

A adsorção enzimática pode depender do tipo de substrato utilizado e desde que as características do substrato podem mudar com a conversão, é importante que o modelo leve em consideração a variação na quantidade de celulase adsorvida duran…

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