Steve F. Perry
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View article: Evolutionary relationships of Fish Lake Valley Tui Chub Siphateles obesus ssp. (Teleostei, Cypriniformes, Leuciscidae) and a new genus of leuciscid minnows from the Alvord Basin, western United States
Evolutionary relationships of Fish Lake Valley Tui Chub Siphateles obesus ssp. (Teleostei, Cypriniformes, Leuciscidae) and a new genus of leuciscid minnows from the Alvord Basin, western United States Open
The Tui Chubs, Siphateles spp., are found widely across the Great Basin and in some adjacent regions. Nearly all diversity of Siphateles has been consolidated under the name S. bicolor and there are numerous isolated populations of Tui Chu…
View article: P-281 Experience of culturing atypically pronucleated embryos derived from IVF and ICSI in a single large IVF clinic
P-281 Experience of culturing atypically pronucleated embryos derived from IVF and ICSI in a single large IVF clinic Open
Study question How often are 3PN blastocysts diploid? Does this differ when fertilisation uses ICSI or IVF? Is adoption of a culture-all policy potentially beneficial for patients? Summary answer Fewer than half the 3PN blastocysts were tr…
View article: P-273 Blastocysts derived from monopronucleated zygotes are frequently diploid, have potential to produce viable pregnancies, and should not be discarded unless genetic abnormality is confirmed
P-273 Blastocysts derived from monopronucleated zygotes are frequently diploid, have potential to produce viable pregnancies, and should not be discarded unless genetic abnormality is confirmed Open
Study question What proportion of 1PN embryos are diploid and potentially viable? Can morphokinetic measurements help distinguish truly haploid 1PN embryos from those that are diploid? Summary answer The majority of 1PNs that form blastocy…
View article: Evolution and divergence of teleost adrenergic receptors: why sometimes ‘the drugs don't work’ in fish
Evolution and divergence of teleost adrenergic receptors: why sometimes ‘the drugs don't work’ in fish Open
Adrenaline and noradrenaline, released as hormones and/or neurotransmitters, exert diverse physiological functions in vertebrates, and teleost fishes are widely used as model organisms to study adrenergic regulation; however, such investig…
View article: O-181 Utilisation of monopronucleated (1PN) embryos and potential clinical benefits for patients
O-181 Utilisation of monopronucleated (1PN) embryos and potential clinical benefits for patients Open
Study question Is it beneficial to culture 1PN embryos for clinical use? Summary answer Culturing 1PN embryos to blastocyst for potential clinical use is beneficial to all patients, especially those with low prognosis. What is known alread…
View article: The control of breathing in fishes – historical perspectives and the path ahead
The control of breathing in fishes – historical perspectives and the path ahead Open
The study of breathing in fishes has featured prominently in Journal of Experimental Biology (JEB), particularly during the latter half of the past century. Indeed, many of the seminal discoveries in this important sub-field of comparative…
View article: Insights into the control and consequences of breathing adjustments in fishes-from larvae to adults
Insights into the control and consequences of breathing adjustments in fishes-from larvae to adults Open
Adjustments of ventilation in fishes to regulate the volume of water flowing over the gills are critically important responses to match branchial gas transfer with metabolic needs and to defend homeostasis during environmental fluctuations…
View article: Catecholamines modulate the hypoxic ventilatory response of larval zebrafish (<i>Danio rerio</i>)
Catecholamines modulate the hypoxic ventilatory response of larval zebrafish (<i>Danio rerio</i>) Open
The hypoxic ventilatory response (HVR) in fish is an important reflex that aids O2 uptake when low environmental O2 levels constrain diffusion. In developing zebrafish (Danio rerio), the acute HVR is multiphasic, consisting of a rapid incr…
View article: Characterization of two novel ammonia transporters, Hiat1a and Hiat1b, in the teleost model system <i>Danio rerio</i>
Characterization of two novel ammonia transporters, Hiat1a and Hiat1b, in the teleost model system <i>Danio rerio</i> Open
Ammonia excretion in fish excretory epithelia is a complex interplay of multiple membrane transport proteins and mechanisms. Using the model system of zebrafish (Danio rerio) larvae, here we identified three paralogues of a novel ammonia t…
View article: Regulation of heart rate following genetic deletion of the ß1 adrenergic receptor in larval zebrafish
Regulation of heart rate following genetic deletion of the ß1 adrenergic receptor in larval zebrafish Open
Aim Although zebrafish are gaining popularity as biomedical models of cardiovascular disease, our understanding of their cardiac control mechanisms is fragmentary. Our goal was to clarify the controversial role of the ß1‐adrenergic recepto…
View article: Aquatic surface respiration improves survival during hypoxia in zebrafish (<i>Danio rerio</i>) lacking hypoxia-inducible factor 1-α
Aquatic surface respiration improves survival during hypoxia in zebrafish (<i>Danio rerio</i>) lacking hypoxia-inducible factor 1-α Open
Hypoxia-inducible factor 1-α (Hif-1α), an important transcription factor regulating cellular responses to reductions in O 2 , previously was shown to improve hypoxia tolerance in zebrafish ( Danio rerio ). Here, we examined the contributio…
View article: The evolutionary and physiological significance of the Hif pathway in teleost fishes
The evolutionary and physiological significance of the Hif pathway in teleost fishes Open
The hypoxia-inducible factor (HIF) pathway is a key regulator of cellular O2 homeostasis and an important orchestrator of the physiological responses to hypoxia (low O2) in vertebrates. Fish can be exposed to significant and frequent chang…
View article: Correction: Reassessing the contribution of the Na+/H+ exchanger Nhe3b to Na+ uptake in zebrafish (<i>Danio rerio</i>) using CRISPR/Cas9 gene editing
Correction: Reassessing the contribution of the Na+/H+ exchanger Nhe3b to Na+ uptake in zebrafish (<i>Danio rerio</i>) using CRISPR/Cas9 gene editing Open
There was an error published in Journal of Experimental Biology (2020) 223, jeb215111 (doi:10.1242/jeb.215111).In Materials and Methods, 'Morpholino KD', the morpholino sequence targeting slc9a3.2 (Nhe3b) was incorrectly written as 5′-CAGT…
View article: Expression of ion transport genes in ionocytes isolated from larval zebrafish (<i>Danio rerio</i>) exposed to acidic or Na<sup>+</sup>-deficient water
Expression of ion transport genes in ionocytes isolated from larval zebrafish (<i>Danio rerio</i>) exposed to acidic or Na<sup>+</sup>-deficient water Open
In zebrafish ( Danio rerio), a specific ionocyte subtype, the H + -ATPase-rich (HR) cell, is presumed to be a significant site of transepithelial Na + uptake/acid secretion. During acclimation to environments differing in ionic composition…
View article: The role of TASK-2 channels in CO<sub>2</sub>sensing in zebrafish (<i>Danio rerio</i>)
The role of TASK-2 channels in CO<sub>2</sub>sensing in zebrafish (<i>Danio rerio</i>) Open
Peripheral chemosensitivity in fishes is thought to be mediated by serotonin-enriched neuroepithelial cells (NECs) that are localized to the gills of adults and the integument of larvae. In adult zebrafish ( Danio rerio), branchial NECs ar…
View article: Hypoxia inducible factor-1 <i>α</i> knockout does not impair acute thermal tolerance or heat hardening in zebrafish
Hypoxia inducible factor-1 <i>α</i> knockout does not impair acute thermal tolerance or heat hardening in zebrafish Open
The rapid increase in critical thermal maximum (CT max ) in fish (or other animals) previously exposed to critically high temperature is termed ‘heat hardening’, which likely represents a key strategy to cope with increasingly extreme envi…
View article: Loss of hypoxia-inducible factor 1α affects hypoxia tolerance in larval and adult zebrafish (<i>Danio rerio</i>)
Loss of hypoxia-inducible factor 1α affects hypoxia tolerance in larval and adult zebrafish (<i>Danio rerio</i>) Open
The coordination of the hypoxic response is attributed, in part, to hypoxia-inducible factor 1α (Hif-1α), a regulator of hypoxia-induced transcription. After the teleost-specific genome duplication, most teleost fishes lost the duplicate c…
View article: Respirometry and cutaneous oxygen flux measurements reveal a negligible aerobic cost of ion regulation in larval zebrafish (<i>Danio rerio</i>)
Respirometry and cutaneous oxygen flux measurements reveal a negligible aerobic cost of ion regulation in larval zebrafish (<i>Danio rerio</i>) Open
Fishes living in fresh water counter the passive loss of salts by actively absorbing ions through specialized cells termed ionocytes. Ionocytes contain ATP-dependent transporters, are enriched with mitochondria, and therefore ionic regulat…
View article: Supplementary material from "Hypoxia inducible factor-1<i>α</i> knockout does not impair acute thermal tolerance or heat hardening in zebrafish"
Supplementary material from "Hypoxia inducible factor-1<i>α</i> knockout does not impair acute thermal tolerance or heat hardening in zebrafish" Open
The rapid increase in critical thermal maximum (CTmax) in fish (or other animals), previously exposed to critically high temperature, is termed ‘heat hardening’, which likely represents a key strategy to cope with increasingly extreme envi…
View article: Role of internal convection in respiratory gas transfer and aerobic metabolism in larval zebrafish (<i>Danio rerio</i>)
Role of internal convection in respiratory gas transfer and aerobic metabolism in larval zebrafish (<i>Danio rerio</i>) Open
Purely diffusive O 2 transport typically is insufficient to sustain aerobic metabolism in most multicellular organisms. In animals that are small enough, however, a high surface-to-volume ratio may allow passive diffusion alone to supply s…
View article: Evaluating the physiological significance of hypoxic hyperventilation in larval zebrafish (<i>Danio rerio</i>)
Evaluating the physiological significance of hypoxic hyperventilation in larval zebrafish (<i>Danio rerio</i>) Open
In water-breathing fishes, the hypoxic ventilatory response (HVR) represents an increase in water flow over the gills during exposure to lowered ambient O2 levels. The HVR is a critical defense mechanism that serves to delay the negative c…
View article: Reassessing the contribution of the Na+/H+ exchanger Nhe3b to Na+ uptake in zebrafish (<i>Danio rerio</i>) using CRISPR/Cas9 gene editing
Reassessing the contribution of the Na+/H+ exchanger Nhe3b to Na+ uptake in zebrafish (<i>Danio rerio</i>) using CRISPR/Cas9 gene editing Open
Freshwater fishes absorb Na+ from their dilute environment using ion-transporting cells. In larval zebrafish (Danio rerio), Na+ uptake is coordinated by (1) Na+/H+-exchanger 3b (Nhe3b) and (2) H+-ATPase-powered electrogenic uptake in H+-AT…
View article: Conflict and Compromise: Using Reversible Remodeling to Manage Competing Physiological Demands at the Fish Gill
Conflict and Compromise: Using Reversible Remodeling to Manage Competing Physiological Demands at the Fish Gill Open
The structural features of the fish gill necessary for oxygen uptake also favor undesirable, passive movements of ions and water. Reversible gill remodeling is one solution to this conflict. Cell masses that limit functional surface area a…
View article: Hif-1α paralogs play a role in the hypoxic ventilatory response of larval and adult zebrafish (<i>Danio rerio</i>)
Hif-1α paralogs play a role in the hypoxic ventilatory response of larval and adult zebrafish (<i>Danio rerio</i>) Open
Hypoxia inducible factor (Hif) 1α, an extensively studied transcription factor, is involved in the regulation of many biological processes in hypoxia including the hypoxic ventilatory response. In zebrafish, there are two paralogs of Hif-1…
View article: A role for sodium-chloride cotransporters in the rapid regulation of ion uptake following acute environmental acidosis: new insights from the zebrafish model
A role for sodium-chloride cotransporters in the rapid regulation of ion uptake following acute environmental acidosis: new insights from the zebrafish model Open
The effects of acute exposure to acidic water on Na + and Cl − homeostasis, and the mechanisms underlying their compensatory regulation, were investigated in the larval zebrafish Danio rerio. Exposure to acidic water (pH 4.0; control pH 7.…
View article: Evidence for a role of heme oxygenase-1 in the control of cardiac function in zebrafish (<i>Danio rerio</i>) larvae exposed to hypoxia
Evidence for a role of heme oxygenase-1 in the control of cardiac function in zebrafish (<i>Danio rerio</i>) larvae exposed to hypoxia Open
Carbon monoxide (CO) is a gaseous neurotransmitter produced from the breakdown of heme via heme oxygenase-1 (HO-1; hypoxia inducible isoform) and 2 (HO-2; constitutively expressed isoform). In mammals, CO is involved in modulating cardiac …
View article: Inhibition of calcium uptake during hypoxia in developing zebrafish, <i>Danio rerio,</i> is mediated by hypoxia-inducible factor
Inhibition of calcium uptake during hypoxia in developing zebrafish, <i>Danio rerio,</i> is mediated by hypoxia-inducible factor Open
The present study investigated the potential role of hypoxia-inducible factor (HIF) on calcium homeostasis in developing zebrafish (Danio rerio). It was demonstrated that zebrafish raised in hypoxic water (30 mmHg; control=155 mmHg) until …
View article: The water channel aquaporin-1a1 facilitates movement of CO2 and ammonia in zebrafish (<i>Danio rerio</i>) larvae
The water channel aquaporin-1a1 facilitates movement of CO2 and ammonia in zebrafish (<i>Danio rerio</i>) larvae Open
The present study tested the hypothesis that zebrafish (Danio rerio) aquaporin-1a1 (AQP1a1) serves as a multi-functional channel for the transfer of the small gaseous molecules, CO2 and ammonia, as well as water, across biological membrane…