Kevin C. Wakeman
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View article: Single-Cell Scanning Electron Microscopy (SEM) v1
Single-Cell Scanning Electron Microscopy (SEM) v1 Open
We present an approach for preparation of single cells for scanning electron microscopy to study uncultivated microbial eukaryotes. Our approach for single-cell scanning electron microscopy preparation involves building a small container, …
View article: Non-photosynthetic Plastid Replacement by a Primary Plastid in the Making
Non-photosynthetic Plastid Replacement by a Primary Plastid in the Making Open
The integration of a cyanobacterium into a heterotrophic eukaryote gave rise to the primary plastid ~1.5 Gya. This rare cyanobacterium-to-plastid transition has only been reported one more time in Paulinella chromatophora ~100 Mya. Unfortu…
View article: Single–Cell Transmission Electron Microscopy (TEM) v1
Single–Cell Transmission Electron Microscopy (TEM) v1 Open
We present an approach for preparing uncultivated protists for transmission electron microscopy. This approach requires building a small container, hereby referred to as the “TEM fixation plate”, that houses individually isolated cells.Thi…
View article: Divergent Plastid Genomes in the Deepest-Branching Apicomplexan Parasites
Divergent Plastid Genomes in the Deepest-Branching Apicomplexan Parasites Open
Apicomplexans are widespread and diverse obligate symbionts of animals, and many—like the malaria-causing Plasmodium—are important human parasites. Some of the closest free-living relatives of apicomplexans are photosynthetic, and most api…
View article: Comparative Sensitivity of MRI Indices for Myelin Assessment in Spinal Cord Regions
Comparative Sensitivity of MRI Indices for Myelin Assessment in Spinal Cord Regions Open
Background/Objectives: Although multiple magnetic resonance imaging (MRI) indices are known to be sensitive to the noninvasive assessment of myelin integrity, their relative sensitivities have not been directly compared. This study aimed t…
View article: Ultrastructure of <i>Olkasia polycarbonata</i> (Euglenozoa, Euglenida) demonstrates cytoskeletal innovations associated with the feeding and flagellar apparatuses
Ultrastructure of <i>Olkasia polycarbonata</i> (Euglenozoa, Euglenida) demonstrates cytoskeletal innovations associated with the feeding and flagellar apparatuses Open
Euglenids are flagellates with diverse modes of nutrition, including the photosynthetic Euglenophyceae, which acquired plastids via secondary endosymbiosis with green algae, and a diverse assemblage of predators of bacteria and other micro…
View article: Phylogenomic diversity of archigregarine apicomplexans
Phylogenomic diversity of archigregarine apicomplexans Open
Gregarines are a large and diverse subgroup of Apicomplexa, a lineage of obligate animal symbionts including pathogens such as Plasmodium , the malaria parasite. Unlike Plasmodium , however, gregarines are poorly studied, despite the fact …
View article: Marine microturbellarians from Japan, with descriptions of two new species of Reinhardorhynchus (Platyhelminthes, Rhabdocoela, Koinocystididae)
Marine microturbellarians from Japan, with descriptions of two new species of Reinhardorhynchus (Platyhelminthes, Rhabdocoela, Koinocystididae) Open
Marine microturbellarians are an assemblage of meiofaunal flatworms abundant in sediments and on seaweeds around the world. The diversity and distribution of these animals in Japan are poorly understood. Here, we provide an overview of all…
View article: GINSA: an accumulator for paired locality and next-generation small ribosomal subunit sequence data
GINSA: an accumulator for paired locality and next-generation small ribosomal subunit sequence data Open
Motivation Motivated by the challenges of decentralized genetic data spread across multiple international organizations, GINSA leverages the Global Biodiversity Information Facility infrastructure to automatically retrieve and link small r…
View article: Parallel functional reduction in the mitochondria of apicomplexan parasites
Parallel functional reduction in the mitochondria of apicomplexan parasites Open
Extreme functional reduction of mitochondria has taken place in parallel in many distantly related lineages of eukaryotes, leading to a number of recurring metabolic states with variously lost electron transport chain (ETC) complexes, loss…
View article: Coral geometry and why it matters
Coral geometry and why it matters Open
Clonal organisms like reef building corals exhibit a wide variety of colony morphologies and geometric shapes which can have many physiological and ecological implications. Colony geometry can dictate the relationship between dimensions of…
View article: Taxonomic study of the polyphyletic <scp><i>Dudresnaya</i></scp> (Dumontiaceae, Florideophyceae) with descriptions of <scp><i>Dudresnaya ryukyuensis</i></scp> sp. nov. and two new genera, <scp><i>Himehibirhodia</i></scp> and <scp><i>Nudresdaya</i></scp>
Taxonomic study of the polyphyletic <span><i>Dudresnaya</i></span> (Dumontiaceae, Florideophyceae) with descriptions of <span><i>Dudresnaya ryukyuensis</i></span> sp. nov. and two new genera, <span><i>Himehibirhodia</i></span> and <span><i>Nudresdaya</i></span> Open
SUMMARY The red algal genus Dudresnaya (Dumontiaceae, Gigartinales) has traditionally been a morphologically well‐defined taxon, but its molecular phylogeny has rarely been studied. To examine the phylogenetic relationships among Dudresnay…
View article: Patterns of host-parasite associations between marine meiofaunal flatworms (Platyhelminthes) and rhytidocystids (Apicomplexa)
Patterns of host-parasite associations between marine meiofaunal flatworms (Platyhelminthes) and rhytidocystids (Apicomplexa) Open
View article: Biodiversity of symbiotic microalgae associated with meiofaunal marine acoels in Southern Japan
Biodiversity of symbiotic microalgae associated with meiofaunal marine acoels in Southern Japan Open
Acoels in the family Convolutidae are commonly found with microalgal symbionts. Convolutids can host green algal Tetraselmis and dinoflagellates within the family Symbiodiniaceae and the genus Amphidinium . The diversity of these microalga…
View article: Reconstruction of Plastid Proteomes of Apicomplexans and Close Relatives Reveals the Major Evolutionary Outcomes of Cryptic Plastids
Reconstruction of Plastid Proteomes of Apicomplexans and Close Relatives Reveals the Major Evolutionary Outcomes of Cryptic Plastids Open
Apicomplexans and related lineages comprise many obligate symbionts of animals; some of which cause notorious diseases such as malaria. They evolved from photosynthetic ancestors and transitioned into a symbiotic lifestyle several times, g…
View article: Peer Review #2 of "Evidence from the resurrected family Polyrhabdinidae Kamm, 1922 (Apicomplexa: Gregarinomorpha) supports the epimerite, an attachment organelle, as a major eugregarine innovation (v0.1)"
Peer Review #2 of "Evidence from the resurrected family Polyrhabdinidae Kamm, 1922 (Apicomplexa: Gregarinomorpha) supports the epimerite, an attachment organelle, as a major eugregarine innovation (v0.1)" Open
Background.Gregarines are a major group of apicomplexan parasites of invertebrates.The gregarine classification is largely incomplete because it relies primarily on light microscopy, while electron microscopy and molecular data in the grou…
View article: Parallel functional reduction in the mitochondria of apicomplexan parasites
Parallel functional reduction in the mitochondria of apicomplexan parasites Open
View article: <i>Grappleria corona</i> gen. et sp. nov. (Platyhelminthes: Rhabdocoela: Jenseniidae fam. nov.) and an updated molecular phylogeny of ‘dalyelliid’ and temnocephalid microturbellarians
<i>Grappleria corona</i> gen. et sp. nov. (Platyhelminthes: Rhabdocoela: Jenseniidae fam. nov.) and an updated molecular phylogeny of ‘dalyelliid’ and temnocephalid microturbellarians Open
Species of rhabdocoels within the family Dalyelliidae (Platyhelminthes) generally inhabit fresh water and are rare in marine and brackish environments. Here we describe a new species of marine dalyelliid, Grappleria corona gen. et s…
View article: Molecular phylogeny and ultrastructure of two novel parasitic dinoflagellates,<i>Haplozoon gracile sp. nov</i>. and<i>H. pugnus sp. nov.</i>
Molecular phylogeny and ultrastructure of two novel parasitic dinoflagellates,<i>Haplozoon gracile sp. nov</i>. and<i>H. pugnus sp. nov.</i> Open
This study describes two novel parasitic dinoflagellates:Haplozoon gracile sp. nov. isolated from a bamboo worm (Maldanidae), 'cf.Petaloclymenesp.'sensuKobayashiet al. 2018; and,H. pugnus sp. nov. isolated fromNicomachesp. andNicomache per…
View article: Validation of a universal set of primers to study animal‐associated microeukaryotic communities
Validation of a universal set of primers to study animal‐associated microeukaryotic communities Open
Summary The application of metabarcoding to study animal‐associated microeukaryotes has been restricted because the universal barcode used to study microeukaryotic ecology and distribution in the environment, the Small Subunit of the Ribos…
View article: Dinoflagellate nucleus contains an extensive endomembrane network, the nuclear net
Dinoflagellate nucleus contains an extensive endomembrane network, the nuclear net Open
View article: A universal set of primers to study animal associated microeukaryotic communities
A universal set of primers to study animal associated microeukaryotic communities Open
Background Unlike the study of bacterial microbiomes, the study of the microeukaryotes associated with animals has largely been restricted to visual identification or molecular targeting of particular groups. The application of high-throug…
View article: Microbial arms race: Ballistic “nematocysts” in dinoflagellates represent a new extreme in organelle complexity
Microbial arms race: Ballistic “nematocysts” in dinoflagellates represent a new extreme in organelle complexity Open
Beyond the potent stings of jellyfish, nematocysts have evolved independently in single-celled phytoplankton.
View article: Molecular Phylogeny of the Marine Planktonic Dinoflagellate Oxytoxum and Corythodinium (Peridiniales, Dinophyceae)
Molecular Phylogeny of the Marine Planktonic Dinoflagellate Oxytoxum and Corythodinium (Peridiniales, Dinophyceae) Open
Gómez, Fernando, C. WAKEMAN, Kevin, YAMAGUCHI, Aika, NOZAKI, Hisayoshi (2016): Molecular Phylogeny of the Marine Planktonic Dinoflagellate Oxytoxum and Corythodinium (Peridiniales, Dinophyceae). Acta Protozoologica 55 (4): 239-248, DOI: 10…