Caitlin E. Randolph
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View article: Lesion-remote astrocytes govern microglia-mediated white matter repair
Lesion-remote astrocytes govern microglia-mediated white matter repair Open
Spared regions of the damaged central nervous system undergo dynamic remodelling and exhibit a remarkable potential for therapeutic exploitation1. Lesion-remote astrocytes (LRAs), which interact with viable neurons and glia, undergo reacti…
View article: Sleep-dependent clearance of brain lipids by peripheral blood cells
Sleep-dependent clearance of brain lipids by peripheral blood cells Open
Sleep is typically viewed through a brain-centric lens, with little known about the role of the periphery. Here, we identify a sleep function for peripheral macrophage-like cells (hemocytes) in the Drosophila circulation, showing that hemo…
View article: Impact Induces Phagocytic Defect in Reactive Microglia
Impact Induces Phagocytic Defect in Reactive Microglia Open
Summary We have developed traumatic brain injury (TBI)-on-a-chip in vitro models using primary microglia and neuronal networks and recorded the molecular and cellular changes following impact to represent impact injury. Using a pH-responsi…
View article: Neutral lipid processing in glia is sexually dimorphic and promotes sleep through diacylglycerol catabolism
Neutral lipid processing in glia is sexually dimorphic and promotes sleep through diacylglycerol catabolism Open
Sleep is thought to have a protective role in clearing toxic waste from the brain, which may include processing of damaged lipids. We recently showed that blocking endocytosis in glia increases sleep and report here that this block is asso…
View article: Glial Biologist's Guide to Mass Spectrometry‐Based Lipidomics: A Tutorial From Sample Preparation to Data Analysis
Glial Biologist's Guide to Mass Spectrometry‐Based Lipidomics: A Tutorial From Sample Preparation to Data Analysis Open
Neurological diseases are associated with disruptions in the brain lipidome that are becoming central to disease pathogenesis. Traditionally perceived as static structural support in membranes, lipids are now known to be actively involved …
View article: Lipids: Emerging Players of Microglial Biology
Lipids: Emerging Players of Microglial Biology Open
Lipids are small molecule immunomodulators that play critical roles in maintaining cellular health and function. Microglia, the resident immune cells of the central nervous system, regulate lipid metabolism both in the extracellular enviro…
View article: Senescent glia link mitochondrial dysfunction and lipid accumulation
Senescent glia link mitochondrial dysfunction and lipid accumulation Open
Senescence is a cellular state linked to ageing and age-onset disease across many mammalian species 1,2 . Acutely, senescent cells promote wound healing 3,4 and prevent tumour formation 5 ; but they are also pro-inflammatory, thus chronica…
View article: Comprehensive Lipidomic Automation Workflow using Large Language Models
Comprehensive Lipidomic Automation Workflow using Large Language Models Open
Lipidomics generates large data that makes manual annotation and interpretation challenging. Lipid chemical and structural diversity with structural isomers further complicates annotation. Although, several commercial and open-source softw…
View article: Lesion-remote astrocytes govern microglia-mediated white matter repair
Lesion-remote astrocytes govern microglia-mediated white matter repair Open
Spared regions of the damaged central nervous system undergo dynamic remodeling and exhibit a remarkable potential for therapeutic exploitation. Here, lesion-remote astrocytes (LRAs), which interact with viable neurons, glia and neural cir…
View article: Recent Developments in Machine Learning for Mass Spectrometry
Recent Developments in Machine Learning for Mass Spectrometry Open
Statistical analysis and modeling of mass spectrometry (MS) data have a long and rich history with several modern MS-based applications using statistical and chemometric methods. Recently, machine learning (ML) has experienced a renaissanc…
View article: Amyloid β Induces Lipid Droplet‐Mediated Microglial Dysfunction in Alzheimer’s Disease
Amyloid β Induces Lipid Droplet‐Mediated Microglial Dysfunction in Alzheimer’s Disease Open
Background Several microglia‐expressed genes have emerged as top risk variants for Alzheimer’s disease (AD) that are involved in lipid droplet (LD) formation and processing. Impaired microglial phagocytosis is one of the main proposed outc…
View article: Amyloid β Induces Lipid Droplet-Mediated Microglial Dysfunction in Alzheimer’s Disease
Amyloid β Induces Lipid Droplet-Mediated Microglial Dysfunction in Alzheimer’s Disease Open
Summary Several microglia-expressed genes have emerged as top risk variants for Alzheimer’s disease (AD). Impaired microglial phagocytosis is one of the main proposed outcomes by which these AD-risk genes may contribute to neurodegeneratio…
View article: Identification of Monomethyl Branched-Chain Lipids by a Combination of Liquid Chromatography Tandem Mass Spectrometry and Charge-Switching Chemistries
Identification of Monomethyl Branched-Chain Lipids by a Combination of Liquid Chromatography Tandem Mass Spectrometry and Charge-Switching Chemistries Open
While various mass spectrometric approaches have been applied to lipid analysis, unraveling the extensive structural diversity of lipids remains a significant challenge. Notably, these approaches often fail to differentiate between isomeri…
View article: Identification of monomethyl branched chain lipids by a combination of liquid chromatography tandem mass spectrometry and charge-switching chemistries
Identification of monomethyl branched chain lipids by a combination of liquid chromatography tandem mass spectrometry and charge-switching chemistries Open
While various mass spectrometric approaches have been applied to lipid analysis, unraveling the extensive structural diversity of lipids remains a significant challenge. Notably, these approaches often fail to differentiate between isomeri…
View article: Localization of Carbon–Carbon Double Bond and Cyclopropane Sites in Cardiolipins via Gas-Phase Charge Inversion Reactions
Localization of Carbon–Carbon Double Bond and Cyclopropane Sites in Cardiolipins via Gas-Phase Charge Inversion Reactions Open
Cardiolipins (CLs) are comprised of two phosphatic acid moieties bound to a central glycerol backbone and are substituted with four acyl chains. Consequently, a vast number of distinct CL structures are possible in different biological con…
View article: Proton Transfer Reactions for the Gas-Phase Separation, Concentration, and Identification of Cardiolipins
Proton Transfer Reactions for the Gas-Phase Separation, Concentration, and Identification of Cardiolipins Open
Cardiolipin (CL) analysis demands high specificity, due to the extensive diversity of CL structures, and high sensitivity, due to their low relative abundance within the lipidome. While electrospray ionization mass spectrometry (ESI-MS) is…
View article: Structural Elucidation of Ether Glycerophospholipids Using Gas-Phase Ion/Ion Charge Inversion Chemistry
Structural Elucidation of Ether Glycerophospholipids Using Gas-Phase Ion/Ion Charge Inversion Chemistry Open
Ether lipids represent a unique subclass of glycerophospholipid (GPL) that possess a 1-O-alkyl (i.e., plasmanyl subclass) or a 1-O-alk-1’-enyl (i.e., plasmenyl subclass) group linked at the sn-1 position of the glycerol backbone. As change…
View article: GAS-PHASE ION/ION REACTIONS FOR ENHANCED LIPID ANALYSIS
GAS-PHASE ION/ION REACTIONS FOR ENHANCED LIPID ANALYSIS Open
Heightened awareness regarding the implication of disturbances in lipid metabolism with respect to prevalent human-related pathologies demands analytical techniques that provide unambiguous structural characterization and accurate quantita…
View article: Toward Complete Structure Elucidation of Glycerophospholipids in the Gas Phase through Charge Inversion Ion/Ion Chemistry
Toward Complete Structure Elucidation of Glycerophospholipids in the Gas Phase through Charge Inversion Ion/Ion Chemistry Open
Shotgun lipidomics has recently gained popularity for lipid analysis. Conventionally, shotgun analysis of glycerophospholipids via direct electrospray ionization tandem mass spectrometry (ESI-MS/MS) provides glycerophospholipid (GPL) class…
View article: Generating Fatty Acid Profiles in the Gas Phase: Fatty Acid Identification and Relative Quantitation Using Ion/Ion Charge Inversion Chemistry
Generating Fatty Acid Profiles in the Gas Phase: Fatty Acid Identification and Relative Quantitation Using Ion/Ion Charge Inversion Chemistry Open
Representing the most fundamental lipid class, fatty acids (FA) play vital biological roles serving as energy sources, cellular signaling molecules, and key architectural components of complex lipids. Direct infusion electrospray ionizatio…