Caitlin M. Davis
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View article: Conformations and sequence determinants in the lipid binding of an adhesive peptide derived from <i>Vibrio cholerae</i> biofilm
Conformations and sequence determinants in the lipid binding of an adhesive peptide derived from <i>Vibrio cholerae</i> biofilm Open
Surface adhesion is critical to the survival of pathogenic bacteria both in natural niches and during infections, often via forming matrix-embedded communities called biofilms. We previously identified a 57-amino acid peptide (Bap1-57aa) a…
View article: Phase Separation Tunes the Stability and Dynamics of G-quadruplex and i-Motif DNA in the Nuclei of Living Cells
Phase Separation Tunes the Stability and Dynamics of G-quadruplex and i-Motif DNA in the Nuclei of Living Cells Open
G-quadruplexes and i-motifs are non-canonical DNA structures that are important not only as targets of gene regulation in cancer therapy, but also as nanotools with biomedical applications. However, their apparent in vitro stabilities and …
View article: Biguanides antithetically regulate tumor properties by the dose-dependent mitochondrial reprogramming-driven c-Src pathway
Biguanides antithetically regulate tumor properties by the dose-dependent mitochondrial reprogramming-driven c-Src pathway Open
The biguanide metformin attenuates mitochondrial oxidation and is proposed as an anti-cancer therapy. However, recent clinical studies suggest increased proliferation and fatty acid β-oxidation (FAO) in a subgroup of patients with breast c…
View article: Weak, specific chemical interactions dictate barnase stability in diverse cellular environments
Weak, specific chemical interactions dictate barnase stability in diverse cellular environments Open
It is well-established that in vitro measurements do not reflect protein behaviors in-cell, where macromolecular crowding and chemical interactions modulate protein stability and kinetics. Recent work suggests that peptides and small prote…
View article: Optical photothermal infrared imaging using metabolic probes in biological systems
Optical photothermal infrared imaging using metabolic probes in biological systems Open
Infrared spectroscopy is a powerful tool for identifying biomolecules. In biological systems, infrared spectra provide information on structure, reaction mechanisms, and conformational change of biomolecules. However, the promise of applyi…
View article: Identifying the minimal sets of distance restraints for FRET-assisted protein structural modeling.
Identifying the minimal sets of distance restraints for FRET-assisted protein structural modeling. Open
Proteins naturally occur in crowded cellular environments and interact with other proteins, nucleic acids, and organelles. Since most previous experimental protein structure determination techniques require that proteins occur in idealized…
View article: Oleic acid differentially affects lipid droplet storage of <i>de novo</i> synthesized lipids in hepatocytes and adipocytes
Oleic acid differentially affects lipid droplet storage of <i>de novo</i> synthesized lipids in hepatocytes and adipocytes Open
This work introduces a multiplexed sub-micron vibrational imaging technique to monitor differential effects of oleic acid in adipocytes and hepatocytes.
View article: Exfoliation of a metal–organic framework enabled by post-synthetic cleavage of a dipyridyl dianthracene ligand
Exfoliation of a metal–organic framework enabled by post-synthetic cleavage of a dipyridyl dianthracene ligand Open
A three-dimensional MOF featuring an anthracene dimer ligand analogous to 4,4′-bipyridine undergoes selective dimensional reduction into a two-dimensional framework that can be mechanically exfoliated.
View article: Oleic acid differentially affects<i>de novo</i>lipogenesis in adipocytes and hepatocytes
Oleic acid differentially affects<i>de novo</i>lipogenesis in adipocytes and hepatocytes Open
Lipogenesis is a vital but often dysregulated metabolic pathway. We report super-resolution multiplexed vibrational imaging of lipogenesis rates and pathways using isotopically labelled oleic acid and glucose as probes in live adipocytes a…
View article: Site-specific crosslinking reveals Phosphofructokinase-L inhibition drives self-assembly and attenuation of protein interactions
Site-specific crosslinking reveals Phosphofructokinase-L inhibition drives self-assembly and attenuation of protein interactions Open
View article: Site-Specific Crosslinking Reveals Phosphofructokinase-L Inhibition Drives Self-Assembly and Attenuation of Protein Interactions
Site-Specific Crosslinking Reveals Phosphofructokinase-L Inhibition Drives Self-Assembly and Attenuation of Protein Interactions Open
Phosphofructokinase is the central enzyme in glycolysis and constitutes a highly regulated step. The liver isoform (PFKL) compartmentalizes during activation and inhibition in vitro and in vivo respectively. Compartmentalized PFKL is hypot…
View article: Chemical interactions modulate λ<sub>6‐85</sub>stability in cells
Chemical interactions modulate λ<sub>6‐85</sub>stability in cells Open
Because steric crowding is most effective when the crowding agent is similar in size to the molecule that it acts upon and the average macromolecule inside cells is much larger than a small protein or peptide, steric crowding is not predic…
View article: Estimated Costs of Intervening in Health-Related Social Needs Detected in Primary Care
Estimated Costs of Intervening in Health-Related Social Needs Detected in Primary Care Open
Importance Health-related social needs are increasingly being screened for in primary care practices, but it remains unclear how much additional financing is required to address those needs to improve health outcomes. Objective To estimate…
View article: On the Topic of Academic Achievement, Professionalism, and Burnout in Family Medicine Residents
On the Topic of Academic Achievement, Professionalism, and Burnout in Family Medicine Residents Open
We greatly appreciate the thoughtful points raised in the response 1 to our paper 2 on the burnout and professionalism milestone attainment of family medicine resident physicians, and we appreciate the nuanced analysis of unique burnout co…
View article: Evaluating the Teaching Health Center Graduate Medical Education Model at 10 Years: Practice-Based Outcomes and Opportunities
Evaluating the Teaching Health Center Graduate Medical Education Model at 10 Years: Practice-Based Outcomes and Opportunities Open
CONTEXT: Since 2011, the Teaching Health Center Graduate Medical Education (THC GME) program has sought to expand access to care by training residents in safety net settings. OBJECTIVE: We used a unique data set to examine impact of the TH…
View article: Healthcare Workforce Implications of Physician Student Loan Repayment Funding
Healthcare Workforce Implications of Physician Student Loan Repayment Funding Open
CONTEXT: As the average level of medical education indebtedness rises, physicians look to loan repayment programs like Public Service Loan Forgiveness (PSLF). This increase in PSLF uptake translates to growing taxpayer investment, while se…
View article: 1465. A Randomized Double-Blinded Placebo-Controlled Study to Determine Efficacy of Immunoglobulin Therapy to Treat BK Viremia in Renal Transplant Recipients
1465. A Randomized Double-Blinded Placebo-Controlled Study to Determine Efficacy of Immunoglobulin Therapy to Treat BK Viremia in Renal Transplant Recipients Open
Background BK viremia in renal transplant recipients increases risk of BK virus-associated nephropathy (BKVAN), posing a threat to allograft function. Up to 30% of all renal transplant recipients develop BK viremia and 1-10% will develop B…
View article: Spliceosomal SL1 RNA binding to U1-70K: the role of the extended RRM
Spliceosomal SL1 RNA binding to U1-70K: the role of the extended RRM Open
The RNA recognition motif (RRM) occurs widely in RNA-binding proteins, but does not always by itself support full binding. For example, it is known that binding of SL1 RNA to the protein U1-70K in the U1 spliceosomal particle is reduced wh…
View article: Physician‐ versus <scp>practice‐level</scp> primary care continuity and association with outcomes in Medicare beneficiaries
Physician‐ versus <span>practice‐level</span> primary care continuity and association with outcomes in Medicare beneficiaries Open
Objective To compare physician‐level versus practice‐level primary care continuity and their association with expenditure and acute care utilization among Medicare beneficiaries and evaluate whether continuity of outpatient primary care at…
View article: Increased IL-6 expression precedes reliable viral detection in the rhesus macaque brain during acute SIV infection
Increased IL-6 expression precedes reliable viral detection in the rhesus macaque brain during acute SIV infection Open
Knowledge of immune activation in the brain during acute HIV infection is crucial for the prevention and treatment of HIV-associated neurological disorders. We determined regional brain (basal ganglia, thalamus, and frontal cortex) immune …
View article: Mimicking a Cellular Environment for RNA Folding Studies
Mimicking a Cellular Environment for RNA Folding Studies Open
View article: Mapping Internally and Environmentally Stimulated RNA Foci Formation
Mapping Internally and Environmentally Stimulated RNA Foci Formation Open
View article: Quinary Structure Modulates Consensus Protein Sequence Stability in Cells
Quinary Structure Modulates Consensus Protein Sequence Stability in Cells Open
View article: An in vitro mimic of in‐cell solvation for protein folding studies
An in vitro mimic of in‐cell solvation for protein folding studies Open
Ficoll, an inert macromolecule, is a common in vitro crowder, but by itself it does not reproduce in‐cell stability or kinetic trends for protein folding. Lysis buffer, which contains ions, glycerol as a simple kosmotrope, and mimics small…
View article: Quantifying protein dynamics and stability in a living organism
Quantifying protein dynamics and stability in a living organism Open
As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cell…
View article: Changes to the Intracellular Milieu Control the Population and Residual Structure of Unfolded Proteins
Changes to the Intracellular Milieu Control the Population and Residual Structure of Unfolded Proteins Open
View article: A quantitative connection of experimental and simulated folding landscapes by vibrational spectroscopy
A quantitative connection of experimental and simulated folding landscapes by vibrational spectroscopy Open
We break the barrier between simulation and experiment by comparing identical computed and experimental infrared observables.
View article: Parallel folding pathways of Fip35 WW domain explained by infrared spectra and their computer simulation
Parallel folding pathways of Fip35 WW domain explained by infrared spectra and their computer simulation Open
We present a calculation of the amide I′ infrared (IR) spectra of the folded, unfolded, and intermediate states of the WW domain Fip35, a model system for β‐sheet folding. Using an all‐atom molecular dynamics simulation in which multiple f…
View article: Spliceosomal U1A Protein-SL2 RNA Binding Affinity Decreases in Cells
Spliceosomal U1A Protein-SL2 RNA Binding Affinity Decreases in Cells Open
View article: Towards Three-Color Live-Cell Imaging of Spliceosome Assembly
Towards Three-Color Live-Cell Imaging of Spliceosome Assembly Open