Callie J. Donahue
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View article: A protein-proximity screen reveals Ebola virus co-opts the mRNA decapping complex through the scaffold protein EDC4
A protein-proximity screen reveals Ebola virus co-opts the mRNA decapping complex through the scaffold protein EDC4 Open
The interaction of host and Ebola virus (EBOV) proteins is required for establishing infection of the cell. To identify protein binding partners, a proximity-dependent protein interaction screen was performed for six EBOV proteins. Hits we…
View article: The Ebola NP0VP35 Complex Phase Separates into Inclusion Body-Like Structures
The Ebola NP0VP35 Complex Phase Separates into Inclusion Body-Like Structures Open
Viral inclusion bodies (IBs) are potential sites of viral replication and assembly.How viral IBs form remains poorly defined.Here we describe a combined biophysical and cellular approach to identify the components necessary for IB formatio…
View article: Structure of the Inmazeb cocktail and resistance to escape against Ebola virus
Structure of the Inmazeb cocktail and resistance to escape against Ebola virus Open
Monoclonal antibodies can provide important pre- or post-exposure protection against disease for those not yet vaccinated or in individuals that fail to mount a protective immune response after vaccination. A key concern in use of monother…
View article: CAPG Is Required for Ebola Virus Infection by Controlling Virus Egress from Infected Cells
CAPG Is Required for Ebola Virus Infection by Controlling Virus Egress from Infected Cells Open
The replication of Ebola virus (EBOV) is dependent upon actin functionality, especially at cell entry through macropinocytosis and at release of virus from cells. Previously, major actin-regulatory factors involved in actin nucleation, suc…
View article: CAPG is required for Ebola virus infection by controlling virus egress from infected cells
CAPG is required for Ebola virus infection by controlling virus egress from infected cells Open
Replication of Ebola virus (EBOV) is dependent upon actin functionality, especially at cell entry through macropinocytosis and at release of virus from cells. Previously, major actin-regulatory factors such as Rac1 and ARP2/3, involved in …
View article: Identification of druggable host targets needed for SARS-CoV-2 infection by combined pharmacological evaluation and cellular network directed prioritization both in vitro and in vivo
Identification of druggable host targets needed for SARS-CoV-2 infection by combined pharmacological evaluation and cellular network directed prioritization both in vitro and in vivo Open
Identification of host factors contributing to replication of viruses and resulting disease progression remains a promising approach for development of new therapeutics. Here, we evaluated 6710 clinical and preclinical compounds targeting …
View article: ORF10–Cullin-2–ZYG11B complex is not required for SARS-CoV-2 infection
ORF10–Cullin-2–ZYG11B complex is not required for SARS-CoV-2 infection Open
Significance Understanding the functions of the genes encoded in the SARS-CoV-2 genome is imperative to understanding its pathogenesis. One unique feature of the SARS-CoV-2 genome is ORF10, a small putative protein that was hypothesized to…
View article: Elucidation of remdesivir cytotoxicity pathways through genome-wide CRISPR-Cas9 screening and transcriptomics
Elucidation of remdesivir cytotoxicity pathways through genome-wide CRISPR-Cas9 screening and transcriptomics Open
The adenosine analogue remdesivir has emerged as a frontline antiviral treatment for SARS-CoV-2, with preliminary evidence that it reduces the duration and severity of illness 1 . Prior clinical studies have identified adverse events 1,2 ,…
View article: A Close-Up Look at PCR
A Close-Up Look at PCR Open
The Polymerase Chain Reaction (PCR) is a fundamental laboratory technique that allows for the amplification of many copies of a desired DNA target sequence. Despite its prevalence, undergraduate students often have poor comprehension about…