Carolyn R. Bertozzi
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Antibody-lectin chimeras for glyco-immune checkpoint blockade Open
Despite the curative potential of checkpoint blockade immunotherapy, many patients remain unresponsive to existing treatments. Glyco-immune checkpoints, which involve interactions of cell-surface glycans with lectin, or glycan-binding, imm…
IMMU-51. Treatment efficacy in murine and human glioma models with brain Compartment-Locked IL-12 does not per se rely on influx of peripheral immune cells and induces distinct transcriptome and secretome changes Open
Glioblastoma (GBM) is widely considered unresponsive to immunotherapy due to its immunosuppressive microenvironment and limited immune cell infiltration. However, recent single-cell transcriptomic studies reveal the presence of exhausted, …
Structure and mechanism of the mitochondrial calcium transporter NCLX Open
As a key mitochondrial Ca 2+ transporter, NCLX regulates intracellular Ca 2+ signalling and vital mitochondrial processes 1–3 . The importance of NCLX in cardiac and nervous-system physiology is reflected by acute heart failure and neurode…
Color-neutral and reversible tissue transparency enables longitudinal deep-tissue imaging in live mice Open
Light scattering in biological tissue presents a significant challenge for deep in vivo imaging. Our previous work demonstrated the ability to achieve optical transparency in live mice using intensely absorbing dye molecules, which created…
Thermal Tracks: A Gaussian process-based framework for universal melting curve analysis enabling unconstrained hit identification in thermal proteome profiling experiments. Open
Thermal Tracks is a Python-based statistical framework for analyzing protein thermal stability data that overcomes key limitations of existing thermal proteome profiling (TPP) workflows. Unlike standard approaches that assume sigmoidal mel…
View article: An Integrin-Targeting Peptide Enables Efficient Lysosomal Delivery of Antibody-Drug Conjugates
An Integrin-Targeting Peptide Enables Efficient Lysosomal Delivery of Antibody-Drug Conjugates Open
Antibody-drug conjugates (ADCs) are standard-of-care therapies for several types of cancer, with an expanding representation in clinical development pipelines. A key determinant of efficacy for most ADCs is their ability to internalize int…
View article: Mechanism of O<sub>2</sub> Activation and Cysteine Oxidation by the Unusual Mononuclear Cu(I) Active Site of the Formylglycine-Generating Enzyme
Mechanism of O<sub>2</sub> Activation and Cysteine Oxidation by the Unusual Mononuclear Cu(I) Active Site of the Formylglycine-Generating Enzyme Open
The formylglycine-generating enzyme (FGE) catalyzes the selective oxidation of a peptidyl-cysteine to form formylglycine, a critical cotranslational modification for type I sulfatase activation and a useful bioconjugation handle. Previous …
Color-neutral and reversible tissue transparency enables longitudinal deep-tissue imaging in live mice Open
Light scattering in biological tissue presents a significant challenge for deep in vivo imaging. Our previous work demonstrated the ability to achieve optical transparency in live mice using intensely absorbing dye molecules, which created…
View article: Glycocalyx dysregulation impairs blood–brain barrier in ageing and disease
Glycocalyx dysregulation impairs blood–brain barrier in ageing and disease Open
The blood–brain barrier (BBB) is highly specialized to protect the brain from harmful circulating factors in the blood and maintain brain homeostasis 1,2 . The brain endothelial glycocalyx layer, a carbohydrate-rich meshwork composed prima…
View article: Mapping the nanoscale organization of the human cell surface proteome reveals new functional associations and surface antigen clusters
Mapping the nanoscale organization of the human cell surface proteome reveals new functional associations and surface antigen clusters Open
The cell surface is a dynamic interface that controls cell-cell communication and signal transduction relevant to organ development, homeostasis and repair, immune reactivity, and pathologies driven by aberrant cell surface phenotypes. The…
Isolation and Imaging of Microvessels From Brain Tissue Open
Proper brain function depends on the integrity of the blood-brain barrier (BBB), which is formed by a specialized network of microvessels in the brain. Reliable isolation of these microvessels is crucial for studying BBB composition and fu…
Luminal Cerebrovascular Proteomics Open
Brain endothelial cells, which constitute the cerebrovasculature, form the first interface between the blood and brain and play essential roles in maintaining central nervous system (CNS) homeostasis. These cells exhibit strong apicobasal …
Genetically-targeted photocatalytic organic dyes for spatiotemporally controlled organic synthesis directed by specific living cells Open
Catalytic reactions of a broad range of abiotic molecules and macromolecules are beyond the native capabilities of mammals. Natural enzymes from prokaryotes or plant-based eukaryotes have limited substrate scopes. Therefore, broadening the…
View article: Sialylated glycoproteins suppress immune cell killing by binding to Siglec-7 and Siglec-9 in prostate cancer
Sialylated glycoproteins suppress immune cell killing by binding to Siglec-7 and Siglec-9 in prostate cancer Open
Prostate cancer is the second leading cause of male cancer death in the U.S. Current immune checkpoint inhibitor-based immunotherapies have improved survival for many malignancies; however, they have failed to prolong survival for prostate…
View article: irCLIP-RNP and Re-CLIP reveal patterns of dynamic protein associations on RNA
irCLIP-RNP and Re-CLIP reveal patterns of dynamic protein associations on RNA Open
RNA binding proteins ( RBPs ) control varied processes, including RNA splicing, stability, transport, and translation 1-3 . Dysfunctional RNA-RBP interactions contribute to the pathogenesis of human disease 1,4,5 , however, characterizing …
Deconvolution of Human Urine across the Transcriptome and Metabolome Open
Background Early detection of the cell type changes underlying several genitourinary tract diseases largely remains an unmet clinical need, where existing assays, if available, lack the cellular resolution afforded by an invasive biopsy. W…
Trogocytosis targeting chimeras (TrogoTACs) for targeted protein transfer Open
Cell surface proteins contribute to cellular identity and mediate a myriad of pathological and normal physiological processes. Multiple induced proximity strategies have emerged to manipulate the cell surface proteome, wherein a protein of…
View article: Cancer–stromal cell interactions in breast cancer brain metastases induce glycocalyx-mediated resistance to HER2-targeting therapies
Cancer–stromal cell interactions in breast cancer brain metastases induce glycocalyx-mediated resistance to HER2-targeting therapies Open
Brain metastatic breast cancer is particularly lethal largely due to therapeutic resistance. Almost half of the patients with metastatic HER2-positive breast cancer develop brain metastases, representing a major clinical challenge. We prev…
Decoding the glycoproteome: a new frontier for biomarker discovery in cancer Open
Cancer early detection and treatment response prediction continue to pose significant challenges. Cancer liquid biopsies focusing on detecting circulating tumor cells (CTCs) and DNA (ctDNA) have shown enormous potential due to their non-in…
Directed evolution of genetically encoded LYTACs for cell-mediated delivery Open
Lysosome-targeting chimeras (LYTACs) are a promising therapeutic modality to drive the degradation of extracellular proteins. However, early versions of LYTAC contain synthetic glycopeptides that cannot be genetically encoded. Here, we pre…
View article: Bioorthogonal Metabolic Labeling of the Virulence Factor Phenolic Glycolipid in Mycobacteria
Bioorthogonal Metabolic Labeling of the Virulence Factor Phenolic Glycolipid in Mycobacteria Open
Surface lipids on pathogenic mycobacteria modulate infection outcomes by regulating host immune responses. Phenolic glycolipid (PGL) is a host-modulating surface lipid that varies among clinical Mycobacterium tuberculosis strains. PGL is a…
Celebrating the 10th Anniversary of <i>ACS Central Science</i> Open
ADVERTISEMENT RETURN TO ISSUEEditorialNEXTCelebrating the 10th Anniversary of ACS Central ScienceCarolyn R. BertozziCarolyn R. BertozziMore by Carolyn R. Bertozzihttps://orcid.org/0000-0003-4482-2754 and Kirk S. SchanzeKirk S. SchanzeMore …
A Genome-Wide CRISPR Screen Identifies Sortilin as the Receptor Responsible for Galectin-1 Lysosomal Trafficking Open
Galectins are a family of mammalian glycan-binding proteins that have been implicated as regulators of myriad cellular processes including cell migration, apoptosis, and immune modulation. Several members of this family, such as galectin-1…
Tumor Immune Cell Targeting Chimeras (TICTACs) reprogram tumor-associated macrophages Open
Immune cells in the tumor microenvironment are not only powerful regulators of immunosuppression and tumorigenesis, but also represent a dominant cell type, with tumor-associated macrophages (TAMs) comprising up to 50% of total cell mass i…